{"title":"Current Promotion","description":"\u003cp\u003eNi-NTA resins offer reproducible high binding capacity, single-step chromatography for routine \u0026amp; scale up purification of polyhistidine tagged fusion proteins. ~40 mg his-tagged protein\/mL medium.\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003e","products":[{"product_id":"proteindex-ni-nta-agarose","title":"PROTEINDEX™ HiBond™ Ni-NTA Agarose","description":"\u003cp\u003e\u003cstrong\u003ePROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e™ HiBond™ Ni-NTA Agarose \u003c\/strong\u003eutilizes 4% agarose as the matrix support, and is functionalized with the chelate ligand Nitilotriacetic Acid (NTA), which is charged with nickel ions (Ni\u003csup\u003e2+\u003c\/sup\u003e) to form a very stable octahedral structure with the nickel ion in the center. The structure of Ni-NTA complex is compatible with solutions that contain certain concentration of reducing agents, denaturing agents, detergents and other additives.\u003c\/p\u003e\n\u003cp\u003ePROTEINDEX™ HiBond™ Ni-NTA Agarose can be used to purify 6xHis-tagged proteins expressed in various hosts, such as\u003cem\u003e E.coli\u003c\/em\u003e., yeast, insect cells and mammalian cells.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eProduct Highlights\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eSuited for routine purification of histidine-tagged proteins\u003c\/li\u003e\n\u003cli\u003eHigh yield (typically \u0026gt; 40 mg protein \/mL resins)\u003c\/li\u003e\n\u003cli\u003eLow leakage of nickel ions. Can be re-used for up to 8 preps*\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cspan style=\"color: #666666;\"\u003e*Note: No significant decrease in yield after 8 repetitive uses of resin when recommended buffers are used. Refer to Product Details for compatible reagents.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eApplications\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eManual batch or gravity flow purification\u003c\/li\u003e\n\u003cli\u003eRoutine His tag protein purification for structural biology and functional assays\u003c\/li\u003e\n\u003cli\u003eProtein-protein and protein-nucleic acid interaction studies\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003e\u003c\/span\u003e\u003c\/h4\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eTech Notes\u003c\/span\u003e\u003c\/h4\u003e\n\u003cp\u003e\u003cb\u003eOne-step purification using \u003c\/b\u003e\u003cb\u003eP\u003c\/b\u003e\u003cb\u003eroteIndex\u003c\/b\u003e\u003cb\u003e™ \u003c\/b\u003e\u003cb\u003eHiBond\u003c\/b\u003e\u003cb\u003e™ Ni-NTA \u003c\/b\u003e\u003cb\u003eAgarose 6 Fast Flow resins \u003c\/b\u003e\u003c\/p\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 0px; border-color: #ffffff;\" width=\"30%\"\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Ni-NTA_2.png?v=1496932129\" alt=\"\"\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 0px; border-color: #ffffff;\" width=\"30%\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cstrong\u003eFigure 1. \u003c\/strong\u003eE.coli lysate containing a 50 kDa target protein was directly loaded on HiBond™ Ni-NTA Agarose 6 FF column. The column was then washed sequentially with 0 mM, 5 mM, and 20 mM imidazole solution before elution with 300 mM imidazole. The target protein was analyzed by SDS-PAGE. \u003cb\u003eC\u003c\/b\u003e\u003cb\u003eL\u003c\/b\u003e: raw lysate; \u003cb\u003eFT\u003c\/b\u003e: unbound flow-through; \u003cb\u003eW1\u003c\/b\u003e,\u003cb\u003e W2 \u003c\/b\u003eand \u003cb\u003eW3: \u003c\/b\u003ewashes; \u003cb\u003eE1\u003c\/b\u003e and \u003cb\u003eE2\u003c\/b\u003e: eluate fractions.\n\u003cul\u003e\u003c\/ul\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"200\"\u003e\u003cstrong\u003eLigand\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eNickel\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBinding capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;40 mg 6xHis-tagged protein\/mL medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMatrix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e4% agarose supplied as 50% slurry\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eParticle size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e45 μm - 165 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaximum pressure\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.1 MPa (1 bar)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eRecommended flow rate\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003eGravity flow, \u0026lt;75 cm\/hour\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eChemical compatibility\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003ctable width=\"93%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"37%\" valign=\"top\"\u003eReducing agents\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e5 mM DTE\u003cbr\u003e0.5-1mM DTT\u003cbr\u003e20 mM β-mercaptoethanol\u003cbr\u003e5 mM TCEP\u003cbr\u003e10 mM reduced glutathione (GSH)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"37%\" valign=\"top\"\u003eDenaturing agents\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e8 M urea, 6 M Gua-HCl \u003cbr\u003e(Tested for one week at 40°C)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"37%\" valign=\"top\"\u003eDetergents\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e2% Triton™ X-100 (nonionic)\u003cbr\u003e2% Tween™ 20 (nonionic)\u003cbr\u003e2% NP-40 (nonionic)\u003cbr\u003e2% cholate (anionic)\u003cbr\u003e1% CHAPS (zwitterionic)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"37%\" valign=\"top\"\u003eOther additives\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e500 mM imidazole\u003cbr\u003e20% ethanol\u003cbr\u003e50% glycerol\u003cbr\u003e100 mM Na\u003csub\u003e2\u003c\/sub\u003eSO4\u003cbr\u003e1.5 M NaCl\u003cbr\u003e1 mM EDTA\u003cbr\u003e60 mM citrate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"37%\" valign=\"top\"\u003eCommonly used buffer\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e50 mM sodium phosphate, pH 7.4\u003cbr\u003e100 mM Tris-HCl, pH 7.4\u003cbr\u003e100 mM Tris-acetate, pH 7.4\u003cbr\u003e100 mM HEPES, pH 7.4\u003cbr\u003e100 mM MOPS, pH 7.4\u003cbr\u003e100 mM sodium acetate, pH 4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage buffer\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1x PBS containing 20% ethanol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProduct is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e   \u003cimg alt=\"Keep refrigerated\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Keep-refrigerated_95fa1867-1f0d-47e6-823a-8a0008076e21_small.png?12332456762384261517\" style=\"float: none;\"\u003e   \u003cimg alt=\"Do not freeze\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Do-not-freeze_2_small.png?12332456762384261517\" style=\"float: none;\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003e\u003c\/h5\u003e\n\u003ch4 style=\"color: #ee8012;\"\u003e\u003c\/h4\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"10 mL","offer_id":18927593668,"sku":"11-0224-010","price":115.0,"currency_code":"USD","in_stock":true},{"title":"50 mL","offer_id":18927593732,"sku":"11-0224-050","price":412.0,"currency_code":"USD","in_stock":true},{"title":"100 mL","offer_id":18927593796,"sku":"11-0224-100","price":759.0,"currency_code":"USD","in_stock":true},{"title":"500 mL","offer_id":39291740979243,"sku":"11-0224-500","price":2733.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/11-0224_Ni-NTA.png?v=1483498777"},{"product_id":"proteindex-ni-nta-agarose-6ff","title":"PROTEINDEX™ HiBond™ Ni-NTA Agarose 6FF","description":"\u003cp\u003e\u003cstrong\u003ePROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e\u003c\/strong\u003e™ \u003cb\u003eHiBond\u003c\/b\u003e\u003cb\u003e™ \u003c\/b\u003e\u003cstrong\u003eNi-NTA Agarose\u003c\/strong\u003e \u003cstrong\u003e6FF\u003c\/strong\u003e has been optimized for automated purification applications. It has been widely used in scale-up and preparative purification of histidine-tagged proteins that are expressed in various hosts, such as \u003cem\u003eE.coli\u003c\/em\u003e, yeast, insect cells, and mammalian cells.\u003c\/p\u003e\n\u003cp\u003e        The base matrix of PROTEINDEX™ HiBond™ Ni-NTA Agarose 6FF is highly crosslinked 6% agarose, offering excellent chemical-, and mechanical stability for automated applications. The matrix support is functionalized with the chelate ligand Nitilotriacetic Acid (NTA) in complex with the nickel ions (Ni\u003csup\u003e2+\u003c\/sup\u003e) in a very stable octahedral structure. The structure of Ni-NTA complex is compatible solutions that contain certain concentration of reducing agents, denaturing agents, detergents and other additives.\u003c\/p\u003e\n\u003cp\u003e        The NTA ligand coordinates the Ni\u003csup\u003e2+\u003c\/sup\u003e with four valencies (tetradentate, coordination number 4), and two valencies are available for interaction with imidazole rings of histidine residues. The number of available valencies seems to provide the best combination of binding effectiveness and chemical stability for binding with the His tag. The Ni-NTA ion-chelate complex on our ProteIndex™ HiBond™ Ni-NTA resins has been delicately optimized to ensure the most effective capture for His tag purification of His-tagged proteins. \u003c\/p\u003e\n\u003cp\u003e       \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eProduct Highlights\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eMechanical stable, designed for scaling up His tag purification\u003c\/li\u003e\n\u003cli\u003eHigh yield (typically \u0026gt; 40 mg protein \/mL resins)\u003c\/li\u003e\n\u003cli\u003eLow leakage of nickel ions. Can be re-used for up to 8 preps*\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cspan style=\"color: #666666;\"\u003e*Note: No significant decrease in yield after 8 repetitive uses of resin when recommended buffers are used. Refer to Product Details for compatible reagents.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eApplications\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eAutomated or syringe operations; and manual batch or gravity flow\u003c\/li\u003e\n\u003cli\u003eRoutine His tag protein purification for structural biology and functional assays\u003c\/li\u003e\n\u003cli\u003eScaling up and preparative applications\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eTech Notes\u003c\/span\u003e\u003c\/h4\u003e\n\u003cp\u003e\u003cb\u003eOne-step purification using \u003c\/b\u003e\u003cb\u003eP\u003c\/b\u003e\u003cb\u003eroteIndex\u003c\/b\u003e\u003cb\u003e™ \u003c\/b\u003e\u003cb\u003eHiBond\u003c\/b\u003e\u003cb\u003e™ Ni-NTA \u003c\/b\u003e\u003cb\u003eAgarose 6 Fast Flow resins \u003c\/b\u003e\u003c\/p\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 0px; border-color: #ffffff;\" width=\"35%\"\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Ni-NTA_6FF_2.png?v=1496201543\" alt=\"\" style=\"display: block; margin-left: auto; margin-right: auto;\"\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 0px; border-color: #ffffff;\" width=\"35%\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cstrong\u003eFigure 1.\u003c\/strong\u003e A 15 kDa protein was expressed in E.coli and purified on an automated system using \u003cb\u003eP\u003c\/b\u003e\u003cb\u003eroteIndex\u003c\/b\u003e\u003cb\u003e™ \u003c\/b\u003e\u003cb\u003eHiBond\u003c\/b\u003e\u003cb\u003e™ Ni-NTA \u003c\/b\u003e\u003cb\u003eAgarose 6 Fast Flow resin \u003c\/b\u003eunder native conditions. 50 mL E.coli lysate in 50 mM NaH\u003csub\u003e2\u003c\/sub\u003ePO\u003csub\u003e4\u003c\/sub\u003e, 0.3 M NaCl, 10 mM imidazole was loaded on the column packed with 0.5 mL resins at the speed of 1 mL\/min. The column was then washed with 100 mL 50 mM NaH\u003csub\u003e2\u003c\/sub\u003ePO\u003csub\u003e4\u003c\/sub\u003e, 0.3 M NaCl, 20 mM imidazole at the speed of 1~2 mL\/min. The target protein was eluted with 50 mM NaH\u003csub\u003e2\u003c\/sub\u003ePO\u003csub\u003e4\u003c\/sub\u003e, 0.3 M NaCl, 250 mM imidazole. Proteins were visualized by coomassie blue staining after separation on SDS-PAGE. \u003cb\u003eL\u003c\/b\u003e: raw lysate; \u003cb\u003eF\u003c\/b\u003e: unbound flow-through; \u003cb\u003eE1\u003c\/b\u003e and \u003cb\u003eE2\u003c\/b\u003e: eluate fractions.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 0px; border-color: #ffffff;\" width=\"37%\"\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Ni-NTA_6FF_comparison_with_Ni_sepharose_4.png?v=1497317795\" alt=\"\"\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 0px; border-color: #ffffff;\" width=\"37%\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cstrong\u003eFigure 2.\u003c\/strong\u003e A 25 kDa protein was expressed in E.coli and purified on an automated system using a one-step automated protocol with \u003cb\u003eP\u003c\/b\u003e\u003cb\u003eroteIndex\u003c\/b\u003e\u003cb\u003e™ \u003c\/b\u003e\u003cb\u003eHiBond\u003c\/b\u003e\u003cb\u003e™ Ni-NTA \u003c\/b\u003e\u003cb\u003eAgarose 6 Fast Flow resin \u003c\/b\u003eunder native conditions. 50 mL E.coli lysate in 50 mM NaH\u003csub\u003e2\u003c\/sub\u003ePO\u003csub\u003e4\u003c\/sub\u003e, 0.3 M NaCl, 10 mM imidazole was loaded on the column packed with 0.5 mL resins at the speed of 1 mL\/min. The column was then washed with 100 mL 50 mM NaH\u003csub\u003e2\u003c\/sub\u003ePO\u003csub\u003e4\u003c\/sub\u003e, 0.3 M NaCl, 20 mM imidazole at the speed of 1~2 mL\/min. The target protein was eluted with 50 mM NaH\u003csub\u003e2\u003c\/sub\u003ePO\u003csub\u003e4\u003c\/sub\u003e, 0.3 M NaCl, 250 mM imidazole. A fraction of eluate was analyzed on a SDS-PAGE. Shown were coomassie blue stains of the target proteins purified from columns of Marvelgent’s ProteIndex Ni-NTA 6FF (\u003cstrong\u003eM\u003c\/strong\u003e) and Ni-Sepharose (\u003cstrong\u003eG\u003c\/strong\u003e) resins.\u003cbr\u003e\u003c\/p\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"200\"\u003e\u003cstrong\u003eLigand\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eNickel\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBinding capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u0026gt; 40 mg 6xHis-tagged protein\/mL medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMatrix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e6% highly cross-linked agarose supplied as 50% slurry\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eParticle size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e45 μm - 165 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaximum pressure\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.3 MPa (3 bar)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eChemical compatibility\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003ctable width=\"93%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"37%\" valign=\"top\"\u003eReducing agents\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e5 mM DTE\u003cbr\u003e0.5-1 mM DTT\u003cbr\u003e20 mM β-mercaptoethanol\u003cbr\u003e5 mM TCEP\u003cbr\u003e10 mM reduced glutathione (GSH)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"37%\" valign=\"top\"\u003eDenaturing agents\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e8 M urea, 6 M Gua-HCl \u003cbr\u003e(Tested for one week at 40°C)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"37%\" valign=\"top\"\u003eDetergents\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e2% Triton™ X-100 (nonionic)\u003cbr\u003e2% Tween™ 20 (nonionic)\u003cbr\u003e2% NP-40 (nonionic)\u003cbr\u003e2% cholate (anionic)\u003cbr\u003e1% CHAPS (zwitterionic)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"37%\" valign=\"top\"\u003eOther additives\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e500 mM imidazole\u003cbr\u003e20% ethanol\u003cbr\u003e50% glycerol\u003cbr\u003e100 mM Na\u003csub\u003e2\u003c\/sub\u003eSO4\u003cbr\u003e1.5 M NaCl\u003cbr\u003e1 mM EDTA\u003cbr\u003e60 mM citrate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"37%\" valign=\"top\"\u003eCommonly used buffer\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e50 mM sodium phosphate, pH 7.4\u003cbr\u003e100 mM Tris-HCl, pH 7.4\u003cbr\u003e100 mM Tris-acetate, pH 7.4\u003cbr\u003e100 mM HEPES, pH 7.4\u003cbr\u003e100 mM MOPS, pH 7.4\u003cbr\u003e100 mM sodium acetate, pH 4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage buffer\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1x PBS containing 20% ethanol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProduct is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e   \u003cimg alt=\"Keep refrigerated\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Keep-refrigerated_95fa1867-1f0d-47e6-823a-8a0008076e21_small.png?12332456762384261517\" style=\"float: none;\"\u003e   \u003cimg alt=\"Do not freeze\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Do-not-freeze_2_small.png?12332456762384261517\" style=\"float: none;\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003e\u003cbr\u003e\u003c\/h5\u003e\n\u003ch4 style=\"color: #ee8012;\"\u003e\u003cbr\u003e\u003c\/h4\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"10 mL","offer_id":19000297540,"sku":"11-0225-010","price":126.0,"currency_code":"USD","in_stock":true},{"title":"50 mL","offer_id":19000297604,"sku":"11-0225-050","price":506.0,"currency_code":"USD","in_stock":true},{"title":"100 mL","offer_id":19000297668,"sku":"11-0225-100","price":825.0,"currency_code":"USD","in_stock":true},{"title":"500 mL","offer_id":5704109129767,"sku":"11-0225-500","price":3366.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/11-0225_Ni-NTA_6FF.png?v=1496933028"},{"product_id":"proteindex-ni-nta-agarose-6ff-prepacked-cartridge","title":"PROTEINDEX™ HiBond™ Ni-NTA Agarose 6FF, Prepacked Cartridge","description":"\u003cp\u003e\u003cstrong\u003ePROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e\u003c\/strong\u003e™ \u003cb\u003eHiBond\u003c\/b\u003e\u003cb\u003e™ \u003c\/b\u003e\u003cstrong\u003eNi-NTA Agarose\u003c\/strong\u003e \u003cstrong\u003e6FF\u003c\/strong\u003e has been optimized for automated purification applications. It has been widely used in scale-up and preparative purification of histidine-tagged proteins that are expressed in various hosts, such as \u003cem\u003eE.coli\u003c\/em\u003e, yeast, insect cells, and mammalian cells.\u003c\/p\u003e\n\u003cp\u003e        The base matrix of PROTEINDEX™ HiBond™ Ni-NTA Agarose 6FF is highly crosslinked 6% agarose, offering excellent chemical-, and mechanical stability for automated applications. The matrix support is functionalized with the chelate ligand Nitilotriacetic Acid (NTA) in complex with the nickel ions (Ni\u003csup\u003e2+\u003c\/sup\u003e) in a very stable octahedral structure. The structure of Ni-NTA complex is compatible solutions that contain certain concentration of reducing agents, denaturing agents, detergents and other additives.\u003c\/p\u003e\n\u003cp\u003e        The NTA ligand coordinates the Ni\u003csup\u003e2+\u003c\/sup\u003e with four valencies (tetradentate, coordination number 4), and two valencies are available for interaction with imidazole rings of histidine residues. The number of available valencies seems to provide the best combination of binding effectiveness and chemical stability for binding with the His tag. The Ni-NTA ion-chelate complex on our ProteIndex™ HiBond™ Ni-NTA resins has been delicately optimized to ensure the most effective capture for His tag purification of His-tagged proteins. \u003c\/p\u003e\n\u003cp\u003e        The Prepacked Cartridge has standard interface and can be adapted to most commonly used chromatography systems, such as ÄKTA systems.\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e        Value bundle with a \u003c\/span\u003e\u003ca rel=\"noopener\" title=\"Connector Luer Female \/ 10-32 Male in PEEK\" href=\"https:\/\/marvelgent.com\/products\/connector-1-16-luer-female-10-32-male-peek\" target=\"_blank\"\u003eLuer Female\/10-32 Male Connector in PEEK\u003c\/a\u003e\u003cspan\u003e (Cat# 20-0315) is now available for all pack sizes, providing convenient operation of the pre-packed column with a syringe at an excellent value.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eProduct Highlights\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eConvenient, easy, ready-to-use\u003c\/li\u003e\n\u003cli\u003eMechanical stable, designed for large scale purification of histidine-tagged proteins\u003c\/li\u003e\n\u003cli\u003eHigh yield (typically \u0026gt; 40 mg protein \/mL resins)\u003c\/li\u003e\n\u003cli\u003eLow leakage of nickel ions. Can be re-used for up to 8 preps*\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cspan style=\"color: #666666;\"\u003e*Note: No significant decrease in yield after 8 repetitive uses of resin when recommended buffers are used. Refer to Product Details for compatible reagents.\u003c\/span\u003e\u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eApplications\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eAutomated purifications\u003c\/li\u003e\n\u003cli\u003eRoutine His tag protein purification for structural biology and functional assays\u003c\/li\u003e\n\u003cli\u003eScaling up and preparative applications\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eTech Notes\u003c\/span\u003e\u003c\/h4\u003e\n\u003cp\u003e\u003cb\u003eOne-step purification using \u003c\/b\u003e\u003cb\u003eP\u003c\/b\u003e\u003cb\u003eroteIndex\u003c\/b\u003e\u003cb\u003e™ \u003c\/b\u003e\u003cb\u003eHiBond\u003c\/b\u003e\u003cb\u003e™ Ni-NTA \u003c\/b\u003e\u003cb\u003eAgarose 6 Fast Flow resins \u003c\/b\u003e\u003c\/p\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"35%\" style=\"border: 0px; border-color: #ffffff;\"\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Ni-NTA_6FF_2.png?v=1496201543\"\u003e\u003c\/td\u003e\n\u003ctd width=\"35%\" style=\"border: 0px; border-color: #ffffff;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cstrong\u003eFigure 1.\u003c\/strong\u003e A 15 kDa protein was expressed in E.coli and purified on an automated system using \u003cb\u003eP\u003c\/b\u003e\u003cb\u003eroteIndex\u003c\/b\u003e\u003cb\u003e™ \u003c\/b\u003e\u003cb\u003eHiBond\u003c\/b\u003e\u003cb\u003e™ Ni-NTA \u003c\/b\u003e\u003cb\u003eAgarose 6 Fast Flow resin \u003c\/b\u003eunder native conditions. 50 mL E.coli lysate in 50 mM NaH\u003csub\u003e2\u003c\/sub\u003ePO\u003csub\u003e4\u003c\/sub\u003e, 0.3 M NaCl, 10 mM imidazole was loaded on the column packed with 0.5 mL resins at the speed of 1 mL\/min. The column was then washed with 100 mL 50 mM NaH\u003csub\u003e2\u003c\/sub\u003ePO\u003csub\u003e4\u003c\/sub\u003e, 0.3 M NaCl, 20 mM imidazole at the speed of 1~2 mL\/min. The target protein was eluted with 50 mM NaH\u003csub\u003e2\u003c\/sub\u003ePO\u003csub\u003e4\u003c\/sub\u003e, 0.3 M NaCl, 250 mM imidazole. Proteins were visualized by coomassie blue staining after separation on SDS-PAGE. \u003cb\u003eL\u003c\/b\u003e: raw lysate; \u003cb\u003eF\u003c\/b\u003e: unbound flow-through; \u003cb\u003eE1\u003c\/b\u003e and \u003cb\u003eE2\u003c\/b\u003e: eluate fractions.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"37%\" style=\"border: 0px; border-color: #ffffff;\"\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Ni-NTA_6FF_comparison_with_Ni_sepharose_4.png?v=1497317795\"\u003e\u003c\/td\u003e\n\u003ctd width=\"37%\" style=\"border: 0px; border-color: #ffffff;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cstrong\u003eFigure 2.\u003c\/strong\u003e A 25 kDa protein was expressed in E.coli and purified on an automated system using a one-step automated protocol with \u003cb\u003eP\u003c\/b\u003e\u003cb\u003eroteIndex\u003c\/b\u003e\u003cb\u003e™ \u003c\/b\u003e\u003cb\u003eHiBond\u003c\/b\u003e\u003cb\u003e™ Ni-NTA \u003c\/b\u003e\u003cb\u003eAgarose 6 Fast Flow resin \u003c\/b\u003eunder native conditions. 50 mL E.coli lysate in 50 mM NaH\u003csub\u003e2\u003c\/sub\u003ePO\u003csub\u003e4\u003c\/sub\u003e, 0.3 M NaCl, 10 mM imidazole was loaded on the column packed with 0.5 mL resins at the speed of 1 mL\/min. The column was then washed with 100 mL 50 mM NaH\u003csub\u003e2\u003c\/sub\u003ePO\u003csub\u003e4\u003c\/sub\u003e, 0.3 M NaCl, 20 mM imidazole at the speed of 1~2 mL\/min. The target protein was eluted with 50 mM NaH\u003csub\u003e2\u003c\/sub\u003ePO\u003csub\u003e4\u003c\/sub\u003e, 0.3 M NaCl, 250 mM imidazole. A fraction of eluate was analyzed on a SDS-PAGE. Shown were coomassie blue stains of the target proteins purified from columns of Marvelgent’s ProteIndex Ni-NTA 6FF (\u003cstrong\u003eM\u003c\/strong\u003e) and Ni-Sepharose (\u003cstrong\u003eG\u003c\/strong\u003e) resins.\u003c\/p\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"200\"\u003e\u003cstrong\u003eLigand\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eNickel\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBinding capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;40 mg 6xHis-tagged protein\/mL medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMatrix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e6% highly cross-linked agarose\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eParticle size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e45 μm - 165 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eColumn volume\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1 mL or 5 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaximum pressure\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.3 MPa (3 bar)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eChemical compatibility\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003ctable width=\"93%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"37%\"\u003eReducing agents\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e5 mM DTE\u003cbr\u003e0.5-1 mM DTT\u003cbr\u003e20 mM β-mercaptoethanol\u003cbr\u003e5 mM TCEP\u003cbr\u003e10 mM reduced glutathione (GSH)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"37%\"\u003eDenaturing agents\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e8 M urea, 6 M Gua-HCl \u003cbr\u003e(Tested for one week at 40°C)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"37%\"\u003eDetergents\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e2% Triton™ X-100 (nonionic)\u003cbr\u003e2% Tween™ 20 (nonionic)\u003cbr\u003e2% NP-40 (nonionic)\u003cbr\u003e2% cholate (anionic)\u003cbr\u003e1% CHAPS (zwitterionic)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"37%\"\u003eOther additives\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e500 mM imidazole\u003cbr\u003e20% ethanol\u003cbr\u003e50% glycerol\u003cbr\u003e100 mM Na\u003csub\u003e2\u003c\/sub\u003eSO4\u003cbr\u003e1.5 M NaCl\u003cbr\u003e1 mM EDTA\u003cbr\u003e60 mM citrate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"37%\"\u003eCommonly used buffer\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e50 mM sodium phosphate, pH 7.4\u003cbr\u003e100 mM Tris-HCl, pH 7.4\u003cbr\u003e100 mM Tris-acetate, pH 7.4\u003cbr\u003e100 mM HEPES, pH 7.4\u003cbr\u003e100 mM MOPS, pH 7.4\u003cbr\u003e100 mM sodium acetate, pH 4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eColumn hardware pressure limit\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003e5 bar (0.5 MPa)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage buffer\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1x PBS containing 20% ethanol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProduct is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e   \u003cimg style=\"float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Keep-refrigerated_95fa1867-1f0d-47e6-823a-8a0008076e21_small.png?12332456762384261517\" alt=\"Keep refrigerated\"\u003e   \u003cimg style=\"float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Do-not-freeze_2_small.png?12332456762384261517\" alt=\"Do not freeze\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"5x 1 mL Columns","offer_id":19098127684,"sku":"11-0230-5x1ML","price":172.0,"currency_code":"USD","in_stock":true},{"title":"5x 1 mL Columns with 1 Connector","offer_id":41405616259115,"sku":"11-0230-5x1MLB","price":200.0,"currency_code":"USD","in_stock":true},{"title":"1x 5 mL Column","offer_id":19098127748,"sku":"11-0230-1x5ML","price":149.0,"currency_code":"USD","in_stock":true},{"title":"1x 5 mL Column with 1 Connector","offer_id":41405616291883,"sku":"11-0230-1x5MLB","price":177.0,"currency_code":"USD","in_stock":true},{"title":"5x 5mL Columns","offer_id":19098127812,"sku":"11-0230-5x5ML","price":539.0,"currency_code":"USD","in_stock":true},{"title":"5x 5 mL Columns with 1 Connector","offer_id":41405616324651,"sku":"11-0230-5x5MLB","price":566.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/Ni-NTA_6FF_prepacked_columns.png?v=1496933949"},{"product_id":"magindex-ni-nta-magnetic-agarose-settled-resin","title":"MagINDEX™ Ni NTA Magnetic Agarose, Settled Resin","description":"\u003cp\u003e\u003cstrong\u003eMagINDEX™ Ni-NTA Magnetic Agarose Beads\u003c\/strong\u003e are high-capacity affinity beads engineered for the rapid purification and screening of 6xHis-tagged recombinant proteins. Built on Marvelgent’s proprietary agarose microsphere and surface chemistry platform, these beads combine the high-performance binding of Ni-NTA resin with the speed and simplicity of magnetic separation.\u003cbr\u003eMagnetic bead-based purification streamlines sample processing by eliminating column packing, centrifugation, and gravity-flow handling. Target proteins are captured directly from clarified lysates—whether from bacterial, insect, or mammalian sources—then rapidly separated, washed, and eluted. This format is ideal for:\u003cbr\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eRapid purification\u003c\/strong\u003e of His-tagged proteins (complete in under an hour).\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eParallel processing\u003c\/strong\u003e of multiple samples.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSmall sample volumes \u003c\/strong\u003ewhere loss must be minimized.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAutomation-compatible workflows\u003c\/strong\u003e where easy handling and reproducibility are essential.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eProduct Highlights\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eProprietary Agarose Coating\u003c\/strong\u003e \u003cbr\u003eOur unique coating technology minimizes magnetic particle shedding and leaching. This ensures maximum bead integrity without compromising the rapid magnetic responsiveness required for efficient capture and elution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eOptimized Surface Chemistry \u003c\/strong\u003e\u003cbr\u003eEngineered for efficient ligand coupling and robust Ni-NTA presentation, our surface chemistry provides enhanced capacity for His-tagged proteins.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eRapid Superparamagnetic Response \u003c\/strong\u003e\u003cbr\u003eThe beads respond to magnetic fields in \u0026lt; 2 seconds. This enables quick, clean separation, reduced hands-on time, and efficient processing of multiple samples.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSuperior Purity \u003c\/strong\u003e\u003cbr\u003eBy significantly reducing non-specific binding, the optimized MagINDEX™ surface ensures high-purity results in every eluate.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eVersatile Workflow Compatibility \u003c\/strong\u003e\u003cbr\u003eCompatible with standard tubes, multi-well plates, and automated magnetic separation platforms. This flexibility supports everything from individual research to high-throughput screening.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBroad Buffer Compatibility \u003c\/strong\u003e\u003cbr\u003eStable in a wide range of common purification buffers, including conditions for both native and denaturing His-tag purification.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eStringent QC \u0026amp; Lot-to-Lot Consistency\u003c\/strong\u003e \u003cbr\u003eEach lot is manufactured under highly controlled conditions and tested against rigorous quality standards to ensure reproducible performance across every experiment.\u003cbr\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ctable style=\"width: 100%; background-color: rgb(230, 230, 230);\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%;\"\u003e\u003ciframe src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/MagIndex_Magnetic_Agarose_Resins-3.mp4?14800512398908436554\"\u003e\u003c\/iframe\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 60%;\"\u003eThe superparamagnetic core provides an exceptionally fast response to magnetic fields, ensuring rapid separation with no residual magnetism once the field is removed. Our proprietary agarose-based encapsulation provides a robust shield that protects the magnetic core across a wide range of common protein purification reagents. This specialized design ensures high chemical stability while minimizing non-specific binding for superior sample purity.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eApplications\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eRapid purification \u003c\/strong\u003eof His-tagged recombinant proteins.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSmall-scale expression screening \u003c\/strong\u003eto identify high-yield clones.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh-throughput purification \u003c\/strong\u003efrom bacterial, insect, or mammalian lysates.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePull-down assays\u003c\/strong\u003e using His-tagged bait proteins to study molecular interactions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eNative or denaturing purification\u003c\/strong\u003e (refer to specifications for compatible agent ranges).\u003cbr\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cspan style=\"color: #666666;\"\u003e*Note: No significant decrease in yield after 8 repetitive uses of resin when recommended buffers are used. Refer to Product Details for compatible reagents.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Ni_NTA_magnetic_agarose2_255d0ddd-ed6f-43c0-8a90-139f30da1ffd.png?v=1778436974\" alt=\"\" width=\"237\" height=\"342\" style=\"display: block; margin-left: auto; margin-right: auto;\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFigure 1. Manual purification of an 80 kDa monomeric recombinant protein. \u003c\/strong\u003eAn 80 kDa monomeric His-tagged protein was purified from 50 mL of eukaryotic cell culture spent media using 4 mL of MagINDEX™ Ni-NTA Magnetic Agarose Beads. The sample was incubated for 30 minutes at room temperature with manual magnetic separation. The purified product was eluted with 250 mM imidazole and analyzed by SDS-PAGE under both reducing (+DTT) and non-reducing conditions. The results confirm high-yield recovery and the expected monomeric state of the target protein. Lane 1: Spent medium; Lane 2: Flow-through; Lane 3: Eluate (+DTT); Lane 4: Eluate (non-reduced).\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Ni_NTA_magnetic_agarose3.png?v=1778436977\" style=\"display: block; margin-left: auto; margin-right: auto;\" width=\"423\" height=\"288\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFigure 2. Automated high-throughput purification of His-tagged nanobodies. \u003c\/strong\u003eHis6-tagged nanobody clones (1–10; ~15 kDa) were expressed in HEK293 cells. Spent media (2 mL) were incubated with 800 µL of MagINDEX™ Ni-NTA Magnetic Agarose Beads for 30 minutes at room temperature using an automated magnetic separation platform. Samples were eluted with 250 mM imidazole and analyzed by SDS-PAGE. The gel demonstrates consistent recovery and high purity across all ten clones.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 25%;\"\u003e\u003cstrong\u003eLigand\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 75%;\"\u003eNickel\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 25%;\"\u003e\u003cstrong\u003eBinding capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 75%;\"\u003e40 mg 6xHis-tagged protein\/mL medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 25%;\"\u003e\u003cstrong\u003eMatrix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 75%;\"\u003eSuperparamagnetic agarose beads\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 25%;\"\u003e\u003cstrong\u003eParticle size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 75%;\"\u003e30 μm - 100 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 25%;\"\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 75%;\"\u003e20% suspension\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 25%;\" valign=\"top\"\u003e\u003cstrong\u003eChemical compatibility\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 75%;\"\u003e\n\u003cp\u003e\u003cspan style=\"text-decoration: underline;\"\u003eReducing agents\u003c\/span\u003e:\u003cbr\u003e5 mM DTE, 0.5-1 mM DTT, 20 mM β-mercaptoethanol, 5 mM TCEP, 10 mM reduced glutathione (GSH)\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"text-decoration: underline;\"\u003eDenaturing agents\u003c\/span\u003e:\u003cbr\u003e8 M urea, 6 M Gua-HCl (Tested for one week at 40°C)\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"text-decoration: underline;\"\u003eDetergents\u003c\/span\u003e:\u003cbr\u003e2% Triton™ X-100 (nonionic), 2% Tween™ 20 (nonionic), 2% NP-40 (nonionic), 2% cholate (anionic), 1% CHAPS (zwitterionic)\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"text-decoration: underline;\"\u003eOther additives\u003c\/span\u003e:\u003cbr\u003e500 mM imidazole, 20% ethanol, 50% glycerol, 100 mM Na\u003csub\u003e2\u003c\/sub\u003eSO4, 1.5 M NaCl, 1 mM EDTA, 60 mM citrate\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"text-decoration: underline;\"\u003eCommonly used buffer:\u003c\/span\u003e\u003cbr\u003e50 mM sodium phosphate (pH 7.4), 100 mM Tris-HCl (pH 7.4), 100 mM Tris-acetate (pH 7.4), 100 mM HEPES (pH 7.4), 100 mM MOPS (pH 7.4), 100 mM sodium acetate (pH 4)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 25%;\"\u003e\u003cstrong\u003eStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 75%;\"\u003e2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 25%;\"\u003e\u003cstrong\u003eStorage buffer\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 75%;\"\u003e1x PBS containing 20% ethanol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProduct is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e   \u003cimg style=\"float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Keep-refrigerated_95fa1867-1f0d-47e6-823a-8a0008076e21_small.png?12332456762384261517\" alt=\"Keep refrigerated\" width=\"52\" height=\"50\"\u003e   \u003cimg height=\"30\" width=\"52\" style=\"float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Do-not-freeze_2_small.png?12332456762384261517\" alt=\"Do not freeze\"\u003e\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e \u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"2 mL","offer_id":42661596266539,"sku":"11-0226-002","price":165.0,"currency_code":"USD","in_stock":true},{"title":"10 mL","offer_id":42661497176107,"sku":"11-0226-010","price":750.0,"currency_code":"USD","in_stock":true},{"title":"100 mL","offer_id":42661497241643,"sku":"11-0226-100","price":5250.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Ni-NTA_magnetic_beads-4.png?v=1774387549"}],"url":"https:\/\/marvelgent.com\/collections\/current-promotion\/separation-method_magnet.oembed","provider":"Marvelgent Biosciences","version":"1.0","type":"link"}