{"title":"Featured Products","description":"","products":[{"product_id":"proteindex-rprotein-a-agarose","title":"PROTEINDEX™ rProtein A Agarose, Settled Resin","description":"\u003cp\u003e\u003cstrong\u003ePROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e\u003c\/strong\u003e™ rProtein A Agarose 4 Fast Flow is an affinity chromatography medium designed for easy, one-step purification of immunoglobulins, notably IgG isotypes, as well as Fc region-containing fusion proteins.\u003c\/p\u003e\n\u003cp\u003eProtein A, a bacterial cell wall protein isolated from \u003cem\u003eStaphylococcus aureus\u003c\/em\u003e, binds to the Fc region of most immunoglobulins through interactions with the heavy chain. Native Protein A contains five high affinity IgG binding domains and many other domains with unknown functions.\u003c\/p\u003e\n\u003cp\u003eThe recombinant Protein A is a genetically engineered protein that contains only the five high affinity IgG binding domains. The non-essential domains were removed to minimize nonspecific binding. The recombinant Protein A is produced in \u003ci\u003eE. coli, \u003c\/i\u003eand it essentially binds to immunoglobulins with the same degree of affinity as the native Protein A. rProtein A is capable of binding with high affinity to the Fc region from IgG of several species including human and rabbit.\u003c\/p\u003e\n\u003cp\u003eEnabled by proprietary ProteIndex™ technology, immobilization of recombinant Protein A on 4% agarose resins was very stable and was optimized for high yield in laboratory preparations and scale-up applications.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eProduct Highlights\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eHigh capacity, \u0026gt; 40 mg human IgG \/ mL of settled resins\u003c\/li\u003e\n\u003cli\u003eHigh selectivity for high purity\u003c\/li\u003e\n\u003cli\u003eMinimal ligand leakage, can be reused\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"5%\"\u003e\u003c\/td\u003e\n\u003ctd width=\"35%\"\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/ProteinA4FF_purifying_mAscites_fluid_3_0d442212-1ce9-4a05-8106-1ec2848dd3cb.png?v=1508439312\"\u003e\u003c\/td\u003e\n\u003ctd valign=\"bottom\"\u003e\n\u003cp\u003e\u003cstrong\u003eHigh purity and high capacity of rProtein A 4FF resin. \u003c\/strong\u003e2 mL mouse ascites fluid was loaded on the column containing 0.5 mL settled rProtein A 4 FF resin. Bound immunoglobulin species were eluted with 100 mM Glycine, pH 3.0. Fractions were analyzed by SDS-PAGE.\u003c\/p\u003e\n\u003cp\u003eLanes: (1) Raw ascites fluid; (2) Flow-through; (3) Elution. Note that, heavy and light chains of IgG were mostly absent from the flow-through, indicating that most immunoglobulins were bound to the column.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"200\"\u003e\u003cstrong\u003eLigand\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eRecombinant protein A produced in \u003cem\u003eE.coli\u003c\/em\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBinding capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;40 mg human IgG\/mL medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMatrix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e4% agarose supplied as 50% slurry\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eParticle size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e45 μm - 165 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaximum pressure\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.1 MPa (1 bar)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eChemical stability\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eAll commonly used aqueous buffers, including 6 M guanidine-HCl and 8 M urea.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eRecommended flow rate\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eGravity flow, 100 - 300 cm\/hour\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003epH stability\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003eLong term: pH3 - 9;\u003c\/p\u003e\n\u003cp\u003eShort term: pH2 - 10\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage buffer\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e20% ethanol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProduct is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e   \u003cimg style=\"float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Keep-refrigerated_95fa1867-1f0d-47e6-823a-8a0008076e21_small.png?12332456762384261517\" alt=\"Keep refrigerated\"\u003e   \u003cimg style=\"float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Do-not-freeze_2_small.png?12332456762384261517\" alt=\"Do not freeze\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eDocuments\u003c\/h5\u003e\n\u003ch4\u003eTechnical Note\u003c\/h4\u003e\n\u003cp\u003e🗎    \u003ca rel=\"noopener noreferrer\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Table_Binding_characteristics_of_immunoglobulin-binding_proteins.pdf?6231220717744860621\" title=\"Comparison of binding characteristics of immunoglobulin-binding proteins\" target=\"_blank\"\u003eComparison of Binding Characteristics of Immunoglobulin-Binding Proteins\u003c\/a\u003e\u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"5 mL","offer_id":14587446980,"sku":"11-0202-005","price":159.0,"currency_code":"USD","in_stock":true},{"title":"25 mL","offer_id":14587447044,"sku":"11-0202-025","price":638.0,"currency_code":"USD","in_stock":true},{"title":"100 mL","offer_id":14587447108,"sku":"11-0202-100","price":1716.0,"currency_code":"USD","in_stock":true},{"title":"200 mL","offer_id":5934033633319,"sku":"11-0202-200","price":3190.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/11-0202_ProteinA.png?v=1508438549"},{"product_id":"proteindex-rprotein-a-agarose-4ff-prepacked-cartridge","title":"PROTEINDEX™ rProtein A Agarose 4FF, Prepacked Cartridge","description":"\u003cp\u003e\u003cstrong\u003ePROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e\u003c\/strong\u003e™ rProtein A Agarose 4 Fast Flow is an affinity chromatography medium designed for easy, one-step purification of immunoglobulins, notably IgG isotypes, as well as Fc region-containing fusion proteins.\u003c\/p\u003e\n\u003cp\u003eProtein A, a bacterial cell wall protein isolated from \u003cem\u003eStaphylococcus aureus\u003c\/em\u003e, binds to the Fc region of most immunoglobulins through interactions with the heavy chain. Native Protein A contains five high affinity IgG binding domains and many other domains with unknown functions. The recombinant Protein A coupled to the agarose resin is produced in \u003ci\u003eE. coli\u003c\/i\u003e and contains five high affinity IgG binding domains with other non-essential domains removed to reduce nonspecific binding. Essentially the same as the native Protein A, rProtein A is capable of binding with high affinity to the Fc region from IgG of several species including human and rabbit.\u003c\/p\u003e\n\u003cp\u003eEnabled by proprietary ProteIndex™ technology, immobilization of recombinant Protein A on 4% highly cross-linked agarose resins was very stable and was optimized for high yield in laboratory preparations and scale-up applications.\u003c\/p\u003e\n\u003cp\u003eThe Prepacked Cartridge has standard interface and can be adapted to most commonly used chromatography systems, such as ÄKTA systems.\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eValue bundle with a \u003c\/span\u003e\u003ca rel=\"noopener\" title=\"Connector Luer Female \/ 10-32 Male in PEEK\" href=\"https:\/\/marvelgent.com\/products\/connector-1-16-luer-female-10-32-male-peek\" target=\"_blank\"\u003eLuer Female\/10-32 Male Connector in PEEK\u003c\/a\u003e\u003cspan\u003e (Cat# 20-0315) is now available for all pack sizes, providing convenient operation of the pre-packed column with a syringe at an excellent value.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eProduct Highlights\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eHigh capacity, \u0026gt; 40 mg human IgG \/ mL of settled resins\u003c\/li\u003e\n\u003cli\u003eHigh selectivity for high purity\u003c\/li\u003e\n\u003cli\u003eMinimal ligand leakage, can be reused\u003c\/li\u003e\n\u003cli\u003eReady-to-use, compatible with standard automated systems\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"5%\"\u003e\u003c\/td\u003e\n\u003ctd width=\"35%\"\u003e \u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/ProteinA4FF_purifying_mAscites_fluid_3_0d442212-1ce9-4a05-8106-1ec2848dd3cb.png?v=1508439312\"\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"bottom\"\u003e\n\u003cp\u003e\u003cstrong\u003eHigh purity and high capacity of rProtein A 4FF resin. \u003c\/strong\u003e2 mL mouse ascites fluid was loaded on the column containing 0.5 mL settled rProtein A 4 FF resin. Bound immunoglobulin species were eluted with 100 mM Glycine, pH 3.0. Fractions were analyzed by SDS-PAGE.\u003c\/p\u003e\n\u003cp\u003eLanes: (1) Raw ascites fluid; (2) Flow-through; (3) Elution. Note that, heavy and light chains of IgG were mostly absent from the flow-through, indicating that most immunoglobulins were bound to the column.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"200\"\u003e\u003cstrong\u003eLigand\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eRecombinant protein A produced in \u003cem\u003eE.coli\u003c\/em\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBinding capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;40 mg human IgG\/mL medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMatrix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eHighly cross-linked 4% agarose\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eParticle size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e45 μm - 165 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaximum pressure\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.3 MPa (3 bar)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eChemical stability\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eAll commonly used aqueous buffers, including 6 M guanidine-HCl and 8 M urea.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eRecommended flow rate\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eGravity flow and medium pressure, 30 - 300 cm\/hour\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003epH stability\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003eLong term: pH 3 - 10;\u003c\/p\u003e\n\u003cp\u003eShort term: pH 2 - 11.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage buffer\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e20% ethanol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProduct is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e   \u003cimg style=\"float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Keep-refrigerated_95fa1867-1f0d-47e6-823a-8a0008076e21_small.png?12332456762384261517\" alt=\"Keep refrigerated\"\u003e   \u003cimg style=\"float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Do-not-freeze_2_small.png?12332456762384261517\" alt=\"Do not freeze\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eDocuments\u003c\/h5\u003e\n\u003ch4\u003eTechnical Note\u003c\/h4\u003e\n\u003cp\u003e🗎    \u003ca rel=\"noopener noreferrer\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Table_Binding_characteristics_of_immunoglobulin-binding_proteins.pdf?6231220717744860621\" title=\"Comparison of binding characteristics of immunoglobulin-binding proteins\" target=\"_blank\"\u003eComparison of Binding Characteristics of Immunoglobulin-Binding Proteins\u003c\/a\u003e\u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"5x 1 mL Columns","offer_id":14710543492,"sku":"11-0204-5x1ML","price":572.0,"currency_code":"USD","in_stock":true},{"title":"5x 1 mL Columns with 1 Connector","offer_id":41405719740459,"sku":"11-0204-5x1MLB","price":599.0,"currency_code":"USD","in_stock":true},{"title":"1x 5 mL Column","offer_id":14710543556,"sku":"11-0204-1x5ML","price":555.0,"currency_code":"USD","in_stock":true},{"title":"1x 5 mL Column with 1 Connector","offer_id":41405719773227,"sku":"11-0204-1x5MLB","price":583.0,"currency_code":"USD","in_stock":true},{"title":"5x 5 mL Columns","offer_id":14710543620,"sku":"11-0204-5x5ML","price":2348.0,"currency_code":"USD","in_stock":true},{"title":"5x 5 mL Columns with 1 Connector","offer_id":41405719805995,"sku":"11-0204-5x5MLB","price":2376.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/Marvelgent_pre-packed_cartridges.png?v=1459891864"},{"product_id":"proteindex-rprotein-g-agarose","title":"PROTEINDEX™ rProtein G Agarose","description":"\u003cp\u003e\u003cstrong\u003ePROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e\u003c\/strong\u003e™ rProtein G Agarose is an affinity chromatography medium designed for easy, one-step purification of immunoglobulins, notably most mammallian IgG isotypes, as well as Fc region-containing fusion proteins.\u003c\/p\u003e\n\u003cp\u003eProtein G is derived from \u003cem\u003eStreptococcus\u003c\/em\u003e strain G148. It contains binding sites for the Fc portion of mammalian IgG. Native protein G has 3 IgG binding domains as well as the sites for albumin and cell-surface binding. Our recombinant protein G contains only the IgG binding domains but no albumin recognition region, therefore, it is compatible with purification protocols that require the presence of fetal bovine serum (FBS) or serum albumin proteins.\u003c\/p\u003e\n\u003cp\u003eShowing super binding specificity for IgG species, \u003cstrong\u003ePROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e\u003c\/strong\u003e™ rProtein G Agarose enables single-step purification of monoclonal and polyclonal antibodies with a greater than 95% purity, and is well suited for IgG purification from various sources, including crude samples of tissue cultures and sera.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eProduct Highlights\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eHigh capacity, \u0026gt; 30 mg human IgG \/ mL of settled resins\u003c\/li\u003e\n\u003cli\u003eHigh selectivity for high purity\u003c\/li\u003e\n\u003cli\u003eMinimal ligand leakage, can be reused\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"5%\"\u003e\u003c\/td\u003e\n\u003ctd width=\"45%\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/ProteinG_4FF_purifying_mAscites_fluid_2.png?v=1508441741\" alt=\"\"\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eHigh purity and high capacity of rProtein G 4FF resin. \u003c\/strong\u003e\u003cbr\u003e2 mL mouse ascites fluid was loaded on the column containing 0.5 mL settled rProtein G 4 FF resin. Bound immunoglobulin species were eluted with 100 mM Glycine, pH 3.0. Fractions were analyzed by SDS-PAGE.\u003c\/p\u003e\n\u003cp\u003eLanes: (1) Raw ascites fluid; (2) Flow-through; (3) Elution 1; (4) Elution 2. Note that, heavy and light chains of IgG were mostly absent from the flow-through, indicating that most immunoglobulins were bound to the column.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"200\"\u003e\u003cstrong\u003eLigand\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eRecombinant protein G produced in \u003cem\u003eE.coli\u003c\/em\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBinding capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;30 mg human IgG\/mL medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMatrix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e4% agarose supplied as 50% slurry\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eParticle size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e45 μm - 165 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaximum pressure\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.1 MPa (1 bar)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eChemical stability\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eAll commonly used aqueous buffers, including 6 M guanidine-HCl and 8 M urea.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eRecommended flow rate\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eGravity flow, 100 - 300 cm\/hour\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003epH stability\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003eLong term: pH3 - 9;\u003c\/p\u003e\n\u003cp\u003eShort term: pH2 - 10\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage buffer\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1x PBS containing 20% ethanol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProduct is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e   \u003cimg alt=\"Keep refrigerated\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Keep-refrigerated_95fa1867-1f0d-47e6-823a-8a0008076e21_small.png?12332456762384261517\" style=\"float: none;\"\u003e   \u003cimg alt=\"Do not freeze\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Do-not-freeze_2_small.png?12332456762384261517\" style=\"float: none;\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eDocuments\u003c\/h5\u003e\n\u003ch4\u003eTechnical Note\u003c\/h4\u003e\n\u003cp\u003e🗎    \u003ca title=\"Comparison of binding characteristics of immunoglobulin-binding proteins\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Table_Binding_characteristics_of_immunoglobulin-binding_proteins.pdf?6231220717744860621\" rel=\"noopener noreferrer\" target=\"_blank\"\u003eComparison of Binding Characteristics of Immunoglobulin-Binding Proteins\u003c\/a\u003e\u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"5 mL","offer_id":14711244548,"sku":"11-0205-005","price":159.0,"currency_code":"USD","in_stock":true},{"title":"25 mL","offer_id":14711244612,"sku":"11-0205-025","price":638.0,"currency_code":"USD","in_stock":true},{"title":"100 mL","offer_id":14711244676,"sku":"11-0205-100","price":1716.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/11-0205_ProteinG_Agarose.png?v=1491951858"},{"product_id":"proteindex-rprotein-g-agarose-4-ff-br-prepacked-cartridge","title":"PROTEINDEX™ rProtein G Agarose 4 FF, Prepacked Cartridge","description":"\u003cp\u003e\u003cstrong\u003ePROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e\u003c\/strong\u003e™ rProtein G Agarose 4FF is an affinity chromatography medium designed for easy, one-step purification of immunoglobulins, notably most mammallian IgG isotypes, as well as Fc region-containing fusion proteins.\u003c\/p\u003e\n\u003cp\u003eProtein G is derived from \u003cem\u003eStreptococcus\u003c\/em\u003e strain G148. It contains binding sites for the Fc portion of mammalian IgG. Native protein G has 3 IgG binding domains as well as the sites for albumin and cell-surface binding. Our recombinant protein G contains only the IgG binding domains but no albumin recognition region, therefore, it is compatible with purification protocols that require the presence of fetal bovine serum (FBS) or serum albumin proteins.\u003c\/p\u003e\n\u003cp\u003eShowing super binding specificity for IgG species, \u003cstrong\u003ePROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e\u003c\/strong\u003e™ rProtein G Agarose 4FF enables single-step purification of monoclonal and polyclonal antibodies with a greater than 95% purity, and is well suited for IgG purification from various sources, including crude samples of tissue cultures and sera. With rigid, highly cross-linked matrix support, this product is versatile and well chemical-tolerant. It is readily adaptable to protocols of various formats over a broad range of chemical conditions.\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eValue bundle with a \u003c\/span\u003e\u003ca rel=\"noopener\" title=\"Connector Luer Female \/ 10-32 Male in PEEK\" href=\"https:\/\/marvelgent.com\/products\/connector-1-16-luer-female-10-32-male-peek\" target=\"_blank\"\u003eLuer Female\/10-32 Male Connector in PEEK\u003c\/a\u003e\u003cspan\u003e (Cat# 20-0315) is now available for all pack sizes, providing convenient operation of the pre-packed column with a syringe at an excellent value.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"200\"\u003e\u003cstrong\u003eLigand\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eRecombinant protein G produced in \u003cem\u003eE.coli\u003c\/em\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBinding capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;30 mg human IgG\/mL medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMatrix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eHighly cross-linked 4% agarose\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eParticle size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e45 μm - 165 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaximum pressure\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.3 MPa (3 bar)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eChemical stability\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eAll commonly used aqueous buffers, including 6 M guanidine-HCl and 8 M urea.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eRecommended flow rate\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eGravity flow, 100 - 300 cm\/hour\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003epH stability\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003eLong term: pH3 - 9;\u003c\/p\u003e\n\u003cp\u003eShort term: pH2 - 10\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage buffer\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1x PBS containing 20% ethanol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProduct is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e   \u003cimg style=\"float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Keep-refrigerated_95fa1867-1f0d-47e6-823a-8a0008076e21_small.png?12332456762384261517\" alt=\"Keep refrigerated\"\u003e   \u003cimg style=\"float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Do-not-freeze_2_small.png?12332456762384261517\" alt=\"Do not freeze\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eDocuments\u003c\/h5\u003e\n\u003ch4\u003eTechnical Note\u003c\/h4\u003e\n\u003cp\u003e🗎    \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Table_Binding_characteristics_of_immunoglobulin-binding_proteins.pdf?6231220717744860621\" title=\"Comparison of binding characteristics of immunoglobulin-binding proteins\" target=\"_blank\"\u003eComparison of Binding Characteristics of Immunoglobulin-Binding Proteins\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"1 mL Columns, Pack of 5","offer_id":14876203012,"sku":"11-0209-5x1ML","price":643.0,"currency_code":"USD","in_stock":true},{"title":"1 mL Columns, Pack of 5 with 1 Connector","offer_id":41405714661419,"sku":"11-0209-5x1MLB","price":671.0,"currency_code":"USD","in_stock":true},{"title":"5 mL Column, Pack of 1","offer_id":14876203076,"sku":"11-0209-1x5ML","price":610.0,"currency_code":"USD","in_stock":true},{"title":"5 mL Column, Pack of 1 with 1 Connector","offer_id":41405715906603,"sku":"11-0209-1x5MLB","price":638.0,"currency_code":"USD","in_stock":true},{"title":"5 mL Columns, Pack of 5","offer_id":14876203140,"sku":"11-0209-5x5ML","price":2637.0,"currency_code":"USD","in_stock":true},{"title":"5 mL Columns, Pack of 5 with 1 Connector","offer_id":41405716037675,"sku":"11-0209-5x5MLB","price":2665.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/Marvelgent_pre-packed_cartridges_0aa3b5cc-ca63-442e-8a8c-e8f8548ca6ff.png?v=1459891896"},{"product_id":"proteindex-rprotein-a-alkali-tolerant-agarose-4ff","title":"PROTEINDEX™ Alkali-Tolerant Protein A Agarose 4 Fast Flow","description":"\u003cp\u003e\u003cstrong\u003ePROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e\u003c\/strong\u003e™ rProtein A Agarose 4 Fast Flow is a proprietary affinity chromatography medium designed for easy, one-step purification of immunoglobulins, notably monoclonal antibodies, from samples of larger volumes.\u003c\/p\u003e\n\u003cp\u003eAlkali-tolerant protein A is engineered by gene recombination technology on the base of native protein A. Our Alkali-Tolerant Protein A Agarose 4FF retains ≥95% capacity after 200 times repeated clean-in-place (CIP) cycles with 0.1M NaOH, and approximately 80% of the initial capacity with 0.5M NaOH after 100 times repeated CIP cycles. Alkali-Tolerant Protein A is immobilized onto the cross-linked 4% agarose resin with an orientation that is optimal for immunoglobulin binding. The level of leakage of the ligand during elution is very low.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"200\"\u003e\u003cstrong\u003eLigand\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eRecombinant alkali-tolerant protein A produced in \u003cem\u003eE.coli\u003c\/em\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBinding capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;40 mg rabbit IgG\/mL medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMatrix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eCross-linked 4% agarose supplied as a 50% slurry\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eParticle size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e45 μm - 165 μm, with an average of ~ 90 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaximum pressure\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.3 MPa (3 bar)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eRecommended flow rate\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eGravity flow and medium pressure, 30 - 300 cm\/hour\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eChemical stability\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eAll commonly used aqueous buffers and reagents in the process of antibody purification, including 6 M guanidine-HCl and 8 M urea.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eCleaning-in-place (CIP)\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.1 M ~ 0.5 M NaOH\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage buffer\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e20% ethanol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProduct is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e   \u003cimg alt=\"Keep refrigerated\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Keep-refrigerated_95fa1867-1f0d-47e6-823a-8a0008076e21_small.png?12332456762384261517\" style=\"float: none;\"\u003e   \u003cimg alt=\"Do not freeze\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Do-not-freeze_2_small.png?12332456762384261517\" style=\"float: none;\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eDocuments\u003c\/h5\u003e\n\u003ch4\u003eTechnical Note\u003c\/h4\u003e\n\u003cp\u003e🗎    \u003ca title=\"Comparison of binding characteristics of immunoglobulin-binding proteins\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Table_Binding_characteristics_of_immunoglobulin-binding_proteins.pdf?6231220717744860621\" target=\"_blank\"\u003eComparison of Binding Characteristics of Immunoglobulin-Binding Proteins\u003c\/a\u003e\u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"10 mL","offer_id":16984774020,"sku":"11-0211-010","price":1207.0,"currency_code":"USD","in_stock":true},{"title":"50 mL","offer_id":16984774084,"sku":"11-0211-050","price":3638.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/Protein_At_agarose4FF.png?v=1490749970"},{"product_id":"proteindex-protein-a-alkali-tolerant-agarose-4ff-prepacked-cartridge","title":"PROTEINDEX™ Alkali-Tolerant Protein A Agarose 4FF, Prepacked Cartridge","description":"\u003cp\u003e\u003cstrong\u003ePROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e\u003c\/strong\u003e™ rProtein A Agarose 4 Fast Flow is a proprietary affinity chromatography medium designed for easy, one-step purification of immunoglobulins, notably monoclonal antibodies, from samples of larger volumes.\u003c\/p\u003e\n\u003cp\u003eAlkali-tolerant protein A is engineered by gene recombination technology on the base of native protein A. Our Alkali-Tolerant Protein A Agarose 4FF retains ≥95% capacity after 200 times repeated clean-in-place (CIP) cycles with 0.1M NaOH, and approximately 80% of the initial capacity with 0.5M NaOH after 100 times repeated CIP cycles. Alkali-Tolerant Protein A is immobilized onto the cross-linked 4% agarose resin with an orientation that is optimal for immunoglobulin binding. The level of leakage of the ligand during elution is very low.\u003c\/p\u003e\n\u003cp\u003eThe Prepacked Cartridge has standard interface and can be adapted to most commonly used chromatography systems, such as ÄKTA systems.\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eValue bundle with a \u003c\/span\u003e\u003ca href=\"https:\/\/marvelgent.com\/products\/connector-1-16-luer-female-10-32-male-peek\" title=\"Connector Luer Female \/ 10-32 Male in PEEK\" rel=\"noopener\" target=\"_blank\"\u003eLuer Female\/10-32 Male Connector in PEEK\u003c\/a\u003e\u003cspan\u003e (Cat# 20-0315) is now available for all pack sizes, providing convenient operation of the pre-packed column with a syringe at an excellent value.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"200\"\u003e\u003cstrong\u003eLigand\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eRecombinant alkali-tolerant protein A produced in \u003cem\u003eE.coli\u003c\/em\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBinding capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;40 mg rabbit IgG\/mL medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMatrix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eCross-linked 4% agarose\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eParticle size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e45 μm - 165 μm, with an average size of ~ 90 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaximum pressure\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.3 MPa (3 bar)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eRecommended flow rate\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eGravity flow and medium pressure, 30 - 300 cm\/hour\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eChemical stability\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eAll commonly used aqueous buffers and reagents in the process of antibody purification, including 6 M guanidine-HCl and 8 M urea.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eCleaning-in-place (CIP)\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.1 M ~ 0.5 M NaOH\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage buffer\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e20% ethanol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProduct is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e   \u003cimg style=\"float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Keep-refrigerated_95fa1867-1f0d-47e6-823a-8a0008076e21_small.png?12332456762384261517\" alt=\"Keep refrigerated\"\u003e   \u003cimg style=\"float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Do-not-freeze_2_small.png?12332456762384261517\" alt=\"Do not freeze\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eDocuments\u003c\/h5\u003e\n\u003ch4\u003eTechnical Note\u003c\/h4\u003e\n\u003cp\u003e🗎    \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Table_Binding_characteristics_of_immunoglobulin-binding_proteins.pdf?6231220717744860621\" title=\"Comparison of binding characteristics of immunoglobulin-binding proteins\" target=\"_blank\"\u003eComparison of Binding Characteristics of Immunoglobulin-Binding Proteins\u003c\/a\u003e\u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"5x 1 mL Columns","offer_id":16990807748,"sku":"11-0212-5x1ML","price":643.0,"currency_code":"USD","in_stock":true},{"title":"5x 1 mL Columns with 1 Connector","offer_id":41405722329131,"sku":"11-0212-5x1MLB","price":671.0,"currency_code":"USD","in_stock":true},{"title":"1x 5 mL Column","offer_id":16990807812,"sku":"11-0212-1x5ML","price":610.0,"currency_code":"USD","in_stock":true},{"title":"1x 5 mL Column with 1 Connector","offer_id":41405722361899,"sku":"11-0212-1x5MLB","price":638.0,"currency_code":"USD","in_stock":true},{"title":"5x 5 mL Columns","offer_id":16990807876,"sku":"11-0212-5x5ML","price":2637.0,"currency_code":"USD","in_stock":true},{"title":"5x 5 mL Columns with 1 Connector","offer_id":41405722394667,"sku":"11-0212-5x5MLB","price":2665.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/ProteinAt_column2.png?v=1493134475"},{"product_id":"proteindex-ni-penta-agarose-chemical-tolerant","title":"PROTEINDEX™ Ni-Penta™ Agarose, Chemical Stable","description":"\u003cp\u003e\u003cstrong\u003ePROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e\u003c\/strong\u003e™ \u003cstrong\u003eChemical-Tolerant Ni-Penta™ Agarose\u003c\/strong\u003e is a new immobilized metal ion affinity chromatography (IMAC) medium precharged with nickel ions, designed mainly for purification of low abundant proteins or challenging proteins in conditions where EDTA, DTT, β-ME, and other stabilizing agents are necessary for maintaining protein stability and integrity.\u003c\/p\u003e\n\u003cp\u003eThe nickel ion binds very strongly to the matrix support in complex with a proprietary pentadentate chelating ligand, which immobilizes the nickel ion through 5 sites to form a homogeneous,  chemical-stable structure. This characteristic nickel ion bonding on the Ni-Penta™ resin provides excellent resistance to EDTA, NaOH, β-ME, and other reagents that are restricted in the purification protocol using conventional NTA- and IDA- functionalized resins.\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"http:\/\/marvelgent.com\/pages\/high-selectivity-ni-penta-agarose-resins-for-secreted-proteins\" title=\"Ni-Penta resins for high selective capture of polyHis-tagged proteins\"\u003eRead more on Ni-Penta properties contributing to high selective purification of His-tagged proteins ...\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eProduct Highlights\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eEDTA-resistant\u003c\/li\u003e\n\u003cli\u003eHigh selectivity for high purity\u003c\/li\u003e\n\u003cli\u003eRetained high 6xHis affinity in various buffers\u003c\/li\u003e\n\u003cli\u003eWell suited for direct capture of secreted polyHis-tagged proteins from eukaryotic cell culture supernatant\u003c\/li\u003e\n\u003cli\u003eRecharge-free with minimal nickel ion leakage\u003c\/li\u003e\n\u003cli\u003eRequires less imidazole\u003c\/li\u003e\n\u003cli\u003eNaOH-tolerant\u003c\/li\u003e\n\u003cli\u003eVersatile, ready-to-use with convenient formats\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eApplications:\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cstrong\u003ePurification of secreted His-tagged proteins directly from eukaryotic cell culture supernatants\u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003eOwing to its proprietary ligand for nickel ion immobilization, Ni-Penta™ resin tolerates EDTA very well, and its affinity for His-tag can be retained in presence of EDTA up to 10 mM. To purify secreted proteins that are produced from eukaryotic cell cultures (such as insect High Five™ cells* and Sf9 cells, CHO cells, 293 cells etc.), a step of removing incompatible agents to Ni-NTA resins by dialysis is frequently required prior to loading the material on the column. This is tedious, time-consuming, and labor intensive. By using Ni-Penta resins, sample pretreament can be skipped, and the culture medium can be loaded directly to the column for purification.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cstrong\u003ePurification of His-tagged proteins that require presence of EDTA, DTT, β-ME, and other agents for maximum protein stability and integrity\u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003ePROTEINDEX™ Ni-Penta™ Agarose Resin is compatible with a wide range of buffers including chelating agents (e.g. EDTA) and reducing agents (e.g. DTT), which are incompatible and therefore should be avoid in purification applications using conventional NTA or IDA resins. Ni-Penta™ resin helps to simplify your sample preparation process and allows your target proteins be purified at their preferred conditions.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cstrong\u003eRepetitive uses of the column that require clean-in-place with NaOH but no nickel recharge \u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003eAnti-strip power of the Ni-Penta™ enables repetitive uses of the column without the need to recharge nickel ion, even after multiple cycles of CIP with 1 M NaOH.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cstrong\u003eRestricted imidazole amount in the elution buffer due to the requirements in downstream applications or the target protein's sensitivity to imidazole\u003cbr\u003e\u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003eImidazole is general required to minimize non-specific binding on Ni-NTA resins. Ni-Penta™ Agarose Resin is highly selective for His-tag and therefore does not demand imidazole for minimizing non-specific binding throughout purification. No imidazole is required in the binding buffer, and 40 mM to 100 mM of imidazole can efficiently elute most target proteins.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003e Technical Notes\u003c\/span\u003e\u003c\/h4\u003e\n\u003ch4\u003e 1. EDTA-stable Ni-Penta chemistry for minimal nickel ion leakage\u003c\/h4\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Ni-Penta_8_99b871bb-a5a2-4123-8891-2437bac436d6.png?v=1493146106\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eFigure 1. Strong chelate chemistry for immobilization of nickel ion. \u003c\/b\u003eNi-Penta Agarose 6 FF resins were incubated at room temperature for 2 hours in 5 volumes of a serial dilutions of EDTA from 10 mM to 400 mM. The amount of nickel ions released into the buffer was measured.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eResult:\u003c\/strong\u003e During 2 hours of incubation in 50 mM EDTA, less than 5 % of nickel ions leaked from Ni-Penta resins. In comparison, 50 mM EDTA is sufficient to strip off nickel ions from conventional Ni-NTA resins and has been used routinely to regenerate Ni-NTA resin.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e2. Uncompromised His-tag protein binding in EDTA up to 10 mM\u003c\/h4\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"color: #3d85c6;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Ni-Penta_7_ad68e7f9-16be-43e9-a619-dcfe21d2590f.png?v=1493145893\" alt=\"\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFigure 2. Retained His-tagged protein binding capacity in presence of EDTA.\u003c\/strong\u003e Ni-Penta 6FF resins were added to 20 volumes of E.coli lysate (~0.5 mg\/mL containing a His-tagged protein of 26 kDa in size), in the presence of 2 mM, 5 mM, 10 mM EDTA respectively. Protein binding was then performed at room temperature for 2 hours. After washing 5 times with 20mM sodium phosphate, 0.5 M NaCl, 5 mM imidazole, pH7.4, the target His-tagged protein was eluted with 20mM sodium phosphate, 0.5 M NaCl, 250 mM imidazole, pH7.4. Protein concentration was measured by UV absorbance at OD280.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eResult:\u003c\/strong\u003e In the presence of 10 mM EDTA, the amount of His-tag protein being purified from Ni-Penta resin was similar to those being purified in the presence of less or no EDTA.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e3. Tolerance to reducing agents\u003c\/h4\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Ni-Penta_9.png?v=1491964108\" alt=\"Ni-penta Tolerance to reducing agents\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eFigure 3. Tolerance to reducing agents. \u003c\/b\u003eNi-Penta 6FF resins were added to 20 volumes of \u003ci\u003eE.coli \u003c\/i\u003elysate (~0.5 mg\/mL containing a His-tagged protein of 26 kDa in size), in the presence of various dilutions of reducing agents, including TCEP, DTT, and β-ME respectively. Protein binding was then performed at room temperature for 2 hours. After washing 5 times with 20mM sodium phosphate, 0.5 M NaCl, 5 mM imidazole, pH7.4, the target His-tagged protein was eluted with 20mM sodium phosphate, 0.5 M NaCl, 250 mM imidazole, pH7.4. Protein concentration was measured by UV absorbance at OD280.\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eResult: \u003c\/b\u003eIn the presence of up to 5 mM TCEP, 10 mM DTT, or 50 mM β-ME, the amount of His-tag protein being purified from Ni-Penta resin was similar to those being purified in absence of the respective reducing agents.\u003c\/p\u003e\n\u003cp\u003e(A) The percentage of retained protein binding capacity is plotted against the concentration of individual reducing agents. (B) Representative SDS-PAGE analysis of the eluates.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e*\u003c\/strong\u003e High Five™ is a trade mark of Thermo Fisher Scientific.\u003c\/p\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"200\"\u003e\u003cstrong\u003eLigand\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eNickel\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBinding capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;10 mg 6XHis-tagged protein\/mL medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMatrix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e4% agarose supplied as 50% slurry\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eParticle size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e45 μm - 165 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaximum pressure\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.1 MPa (1 bar)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eRecommended flow rate\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003eGravity flow, \u0026lt;75 cm\/hour\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eChemical compatibility\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cul\u003e\n\u003cli\u003e0.01 M HCl ,0.01 M NaOH, for ≥ 1 week;\u003c\/li\u003e\n\u003cli\u003e10 mM EDTA, 1 M NaOH, 5 mM DTT, 5 mM TCEP, 20 mM β-ME, 6 M guanidine-HCl, for ≥ 24 hours\u003c\/li\u003e\n\u003cli\u003e500 mM imidazole，100 mM EDTA, for ≥ 2 hours\u003c\/li\u003e\n\u003cli\u003e30% isopropanol for ≥ 20 mins\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage buffer\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1X PBS containing 20% ethanol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProduct is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e   \u003cimg alt=\"Keep refrigerated\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Keep-refrigerated_95fa1867-1f0d-47e6-823a-8a0008076e21_small.png?12332456762384261517\" style=\"float: none;\"\u003e   \u003cimg alt=\"Do not freeze\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Do-not-freeze_2_small.png?12332456762384261517\" style=\"float: none;\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eDocuments\u003c\/h5\u003e\n\u003ch4\u003eProduct Information Sheet\u003c\/h4\u003e\n\u003cp\u003e🗎    \u003ca title=\"Man_11-0227_Chemical-Tolerant Ni-Penta Agarose_v1603.pdf\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Man_11-0227_Chemical-Tolerant_Ni-Penta_Agarose_v1603.pdf?9251540950763704712\" target=\"_blank\"\u003eNi-Penta™ Agarose\u003c\/a\u003e\u003c\/p\u003e\n\u003ch4 style=\"color: #ee8012;\"\u003e\u003cbr\u003e\u003c\/h4\u003e\n\u003cp style=\"text-align: left;\"\u003e \u003c\/p\u003e\n\u003ch5\u003eAccessories\u003c\/h5\u003e\n\u003cp\u003e\u003ca title=\"Disposable gravity flow columns\" href=\"http:\/\/marvelgent.com\/collections\/empty-gravity-flow-columns\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/Gravity_columns_730e2b1d-64df-4e61-96c2-f2f8c076c2d6_compact.png?v=1468002569\" alt=\"\"\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca title=\"Disposable gravity flow columns\" href=\"http:\/\/marvelgent.com\/collections\/empty-gravity-flow-columns\"\u003eDisposable Gravity Flow Columns\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"10 mL","offer_id":17018319684,"sku":"11-0227-010","price":146.0,"currency_code":"USD","in_stock":true},{"title":"50 mL","offer_id":17018319812,"sku":"11-0227-050","price":586.0,"currency_code":"USD","in_stock":true},{"title":"100 mL","offer_id":17018319876,"sku":"11-0227-100","price":1051.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/11-0227_Ni-Penta.png?v=1490835874"},{"product_id":"proteindex-ni-penta-agarose-6-fast-flow-chemical-stable-br-prepacked-cartridge","title":"PROTEINDEX™ Ni-Penta™ Agarose 6 Fast Flow, Chemical Stable, Prepacked Cartridge","description":"\u003cp\u003e\u003cstrong\u003eROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e\u003c\/strong\u003e™ \u003cstrong\u003eChemical-Tolerant Ni-Penta™ Agarose 6FF\u003c\/strong\u003e is a new immobilized metal ion affinity chromatography (IMAC) medium precharged with nickel ions, designed mainly for purification of low abundant proteins or challenging proteins in conditions where EDTA, DTT, β-ME, and other stabilizing agents are necessary for maintaining protein stability and integrity.\u003c\/p\u003e\n\u003cp\u003eThe nickel ion binds very strongly to the matrix support in complex with a proprietary pentadentate chelating ligand, which immobilizes the nickel ion through 5 sites to form a homogeneous,  chemical-stable structure. This characteristic nickel ion bonding on the Ni-Penta™ resin provides excellent resistance to EDTA, NaOH, β-ME, and other reagents that are restricted in the purification protocol using conventional NTA- and IDA- functionalized resins. In particular, Ni-Penta™ Agarose 6FF is well suited for direct capture of secreted polyHis-tagged proteins from the cell culture supernatant of eukaryotic cells, including CHO cells, insect High Five™ cells* and Sf9 cells.\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"http:\/\/marvelgent.com\/pages\/high-selectivity-ni-penta-agarose-resins-for-secreted-proteins\" title=\"Ni-Penta resins for high selective capture of polyHis-tagged proteins\"\u003eRead more on Ni-Penta properties contributing to high selective purification of His-tagged proteins ...\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003eNi-Penta™ Agarose 6 FF Prepacked Cartridge are packed with 1 ml and 5 ml of Ni-Penta Agarose 6 Fast Flow. The columns have standard interface that can be adapted to most commonly used chromatography systems, such as ÄKTA.\u003c\/p\u003e\n\u003cp\u003ePrepacked 16\/10 columns and 7\/10 columns offer a bed height of 10 cm that is convenient for process scale-up and development.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eValue bundle with a \u003ca rel=\"noopener\" title=\"Connector Luer Female \/ 10-32 Male in PEEK\" href=\"https:\/\/marvelgent.com\/products\/connector-1-16-luer-female-10-32-male-peek\" target=\"_blank\"\u003eLuer Female\/10-32 Male Connector in PEEK\u003c\/a\u003e (Cat# 20-0315) is now available for all pack sizes, providing convenient operation of the pre-packed column with a syringe at an excellent value.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eProduct Highlights\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eEDTA-resistant\u003c\/li\u003e\n\u003cli\u003eHigh selectivity for high purity\u003c\/li\u003e\n\u003cli\u003eRetained high 6xHis affinity in various buffers\u003c\/li\u003e\n\u003cli\u003eRecharge-free with minimal nickel ion leakage\u003c\/li\u003e\n\u003cli\u003eRequires less imidazole\u003c\/li\u003e\n\u003cli\u003eNaOH-tolerant, allowing CIP with 1 M NaOH\u003c\/li\u003e\n\u003cli\u003eReady-to-use, convenient formats: 1 mL and 5 mL\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eApplications:\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cstrong\u003ePurification of secreted His-tagged proteins directly from eukaryotic cell culture supernatants\u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003eOwing to its proprietary ligand for nickel ion immobilization, Ni-Penta™ resin tolerates EDTA very well, and its affinity for His-tag can be retained in presence of EDTA up to 10 mM. To purify secreted proteins that are produced from eukaryotic cell cultures (such as insect cells, CHO cells, 293 cells etc.), a step of removing incompatible agents to Ni-NTA resins by dialysis is frequently required prior to loading the material on the column. This is tedious, time-consuming, and labor intensive. By using Ni-Penta resins, sample pretreament can be skipped, and the culture medium can be loaded directly to the column for purification.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cstrong\u003ePurification of His-tagged proteins that require presence of EDTA, DTT, β-ME, and other agents for maximum protein stability and integrity\u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003ePROTEINDEX™ Ni-Penta™ Agarose Resin is compatible with a wide range of buffers including chelating agents (e.g. EDTA) and reducing agents (e.g. DTT), which are incompatible and therefore should be avoid in purification applications using conventional NTA or IDA resins. Ni-Penta™ resin helps to simplify your sample preparation process and allows your target proteins be purified at their preferred conditions.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cstrong\u003eRepetitive uses of the column that require clean-in-place with NaOH but no nickel recharge \u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003eAnti-strip power of the Ni-Penta™ enables repetitive uses of the column without the need to recharge nickel ion, even after multiple cycles of CIP with 1 M NaOH.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cstrong\u003eRestricted imidazole amount in the elution buffer due to the requirements in downstream applications or the target protein's sensitivity to imidazole\u003cbr\u003e\u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003eImidazole is general required to minimize non-specific binding on Ni-NTA resins. Ni-Penta™ Agarose Resin is highly selective for His-tag and therefore does not demand imidazole for minimizing non-specific binding throughout purification. No imidazole is required in the binding buffer, and 40 mM to 100 mM of imidazole can efficiently elute most target proteins.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\n\u003cspan style=\"color: #3d85c6;\"\u003e Technical Notes\u003c\/span\u003e \u003c\/h4\u003e\n\u003ch4\u003e1. EDTA-stable Ni-Penta chemistry for minimal nickel ion leakage\u003c\/h4\u003e\n\u003cp style=\"text-align: center;\"\u003e \u003cimg alt=\"EDTA-stable chelate chemistry for immobilization of nickel ion.\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Ni-Penta_8_grande.png?v=1491963528\" style=\"float: none;\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eFigure 1. Strong chelate chemistry for immobilization of nickel ion. \u003c\/b\u003eNi-Penta Agarose 6 FF resins were incubated at room temperature for 2 hours in 5 volumes of a serial dilutions of EDTA from 10 mM to 400 mM. The amount of nickel ions released into the buffer was measured.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eResult:\u003c\/strong\u003e During 2 hours of incubation in 50 mM EDTA, less than 5 % of nickel ions leaked from Ni-Penta resins. In comparison, 50 mM EDTA is sufficient to strip off nickel ions from conventional Ni-NTA resins and has been used routinely to regenerate Ni-NTA resin.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e2. Uncompromised His-tag protein binding in EDTA up to 10 mM\u003c\/h4\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"color: #3d85c6;\"\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Ni-Penta_7_654f089b-e6ea-413d-9b06-abb3ead360a8_grande.png?v=1491962523\" style=\"float: none;\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFigure 2. Retained His-tagged protein binding capacity in presence of EDTA.\u003c\/strong\u003e Ni-Penta 6FF resins were added to 20 volumes of E.coli lysate (~0.5 mg\/mL containing a His-tagged protein of 26 kDa in size), in the presence of 2 mM, 5 mM, 10 mM EDTA respectively. Protein binding was then performed at room temperature for 2 hours. After washing 5 times with 20mM sodium phosphate, 0.5 M NaCl, 5 mM imidazole, pH7.4, the target His-tagged protein was eluted with 20mM sodium phosphate, 0.5 M NaCl, 250 mM imidazole, pH7.4. Protein concentration was measured by UV absorbance at OD280.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eResult:\u003c\/strong\u003e In the presence of 10 mM EDTA, the amount of His-tag protein being purified from Ni-Penta resin was similar to those being purified in the presence of less or no EDTA.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e3. Tolerance to reducing agents\u003c\/h4\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Ni-Penta_9.png?v=1491964108\" alt=\"Ni-penta Tolerance to reducing agents\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eFigure 3. Tolerance to reducing agents. \u003c\/b\u003eNi-Penta 6FF resins were added to 20 volumes of \u003ci\u003eE.coli \u003c\/i\u003elysate (~0.5 mg\/mL containing a His-tagged protein of 26 kDa in size), in the presence of various dilutions of reducing agents, including TCEP, DTT, and β-ME respectively. Protein binding was then performed at room temperature for 2 hours. After washing 5 times with 20mM sodium phosphate, 0.5 M NaCl, 5 mM imidazole, pH7.4, the target His-tagged protein was eluted with 20mM sodium phosphate, 0.5 M NaCl, 250 mM imidazole, pH7.4. Protein concentration was measured by UV absorbance at OD280.\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eResult: \u003c\/b\u003eIn the presence of up to 5 mM TCEP, 10 mM DTT, or 50 mM β-ME, the amount of His-tag protein being purified from Ni-Penta resin was similar to those being purified in absence of the respective reducing agents.\u003c\/p\u003e\n\u003cp\u003e(A) The percentage of retained protein binding capacity is plotted against the concentration of individual reducing agents. (B) Representative SDS-PAGE analysis of the eluates.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e*\u003c\/strong\u003e High Five™ is a trade mark of Thermo Fisher Scientific.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"200\"\u003e\u003cstrong\u003eLigand\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eNickel\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBinding capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;10 mg 6XHis-tagged protein\/mL medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMatrix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eHighly cross-linked 6% agarose resin\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eParticle size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e45 μm - 165 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaximum pressure\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.3 MPa (3 bar)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eChemical compatibility\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cul\u003e\n\u003cli\u003e0.01 M HCl ,0.01 M NaOH, for ≥ 1 week;\u003c\/li\u003e\n\u003cli\u003e10 mM EDTA, 1 M NaOH, 5 mM DTT, 5 mM TCEP, 20 mM β-ME, 6 M guanidine-HCl, for ≥ 24 hours\u003c\/li\u003e\n\u003cli\u003e500 mM imidazole，100 mM EDTA, for ≥ 2 hours\u003c\/li\u003e\n\u003cli\u003e30% isopropanol for ≥ 20 mins\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage buffer\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1x PBS containing 20% ethanol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProduct is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e   \u003cimg alt=\"Keep refrigerated\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Keep-refrigerated_95fa1867-1f0d-47e6-823a-8a0008076e21_small.png?12332456762384261517\" style=\"float: none;\"\u003e   \u003cimg alt=\"Do not freeze\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Do-not-freeze_2_small.png?12332456762384261517\" style=\"float: none;\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eDocuments\u003c\/h5\u003e\n\u003ch4\u003eProduct Information Sheet\u003c\/h4\u003e\n\u003cp\u003e🗎    \u003ca title=\"Man_11-0228_Chemical-Tolerant Ni-Penta Agarose6FF Prepacked Cartridges_v1603.pdf\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Man_11-0229_Chemical-Tolerant_Ni-Penta_Agarose_6_FF_Prepacked_Cartridge_v1603.pdf?9251540950763704712\" rel=\"noopener noreferrer\" target=\"_blank\"\u003eNi-Penta™ Agarose 6FF, Prepacked Cartridges\u003c\/a\u003e\u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"1 mL Columns, Pack of 5","offer_id":41405565042731,"sku":"11-0229-5x1ML","price":177.0,"currency_code":"USD","in_stock":true},{"title":"1 mL Columns, Pack of 5 with 1 Connector","offer_id":41405559537707,"sku":"11-0229-5x1MLB","price":204.0,"currency_code":"USD","in_stock":true},{"title":"5 mL Column, Pack of 1","offer_id":41405388554283,"sku":"11-0229-1x5ML","price":151.0,"currency_code":"USD","in_stock":true},{"title":"5 mL Column, Pack of 1,  with 1 Connector","offer_id":41405562585131,"sku":"11-0229-1x5MLB","price":179.0,"currency_code":"USD","in_stock":true},{"title":"5 mL Columns, Pack of 5","offer_id":41405565173803,"sku":"11-0229-5x5ML","price":586.0,"currency_code":"USD","in_stock":true},{"title":"5 mL Columns, Pack of 5, with 1 Connector","offer_id":41405388619819,"sku":"11-0229-5x5MLB","price":613.0,"currency_code":"USD","in_stock":true},{"title":"16\/10 Column, 20 mL","offer_id":42112519569451,"sku":"11-0229-C20","price":709.0,"currency_code":"USD","in_stock":true},{"title":"7\/10 Columns, 4.7 mL, Pack of 2","offer_id":42112598048811,"sku":"11-0229-C47","price":544.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/11-0229_Ni-Penta_cartridges.png?v=1493997900"},{"product_id":"proteindex-ni-nta-agarose","title":"PROTEINDEX™ HiBond™ Ni-NTA Agarose","description":"\u003cp\u003e\u003cstrong\u003ePROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e™ HiBond™ Ni-NTA Agarose \u003c\/strong\u003eutilizes 4% agarose as the matrix support, and is functionalized with the chelate ligand Nitilotriacetic Acid (NTA), which is charged with nickel ions (Ni\u003csup\u003e2+\u003c\/sup\u003e) to form a very stable octahedral structure with the nickel ion in the center. The structure of Ni-NTA complex is compatible with solutions that contain certain concentration of reducing agents, denaturing agents, detergents and other additives.\u003c\/p\u003e\n\u003cp\u003ePROTEINDEX™ HiBond™ Ni-NTA Agarose can be used to purify 6xHis-tagged proteins expressed in various hosts, such as\u003cem\u003e E.coli\u003c\/em\u003e., yeast, insect cells and mammalian cells.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eProduct Highlights\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eSuited for routine purification of histidine-tagged proteins\u003c\/li\u003e\n\u003cli\u003eHigh yield (typically \u0026gt; 40 mg protein \/mL resins)\u003c\/li\u003e\n\u003cli\u003eLow leakage of nickel ions. Can be re-used for up to 8 preps*\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cspan style=\"color: #666666;\"\u003e*Note: No significant decrease in yield after 8 repetitive uses of resin when recommended buffers are used. Refer to Product Details for compatible reagents.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eApplications\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eManual batch or gravity flow purification\u003c\/li\u003e\n\u003cli\u003eRoutine His tag protein purification for structural biology and functional assays\u003c\/li\u003e\n\u003cli\u003eProtein-protein and protein-nucleic acid interaction studies\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003e\u003c\/span\u003e\u003c\/h4\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eTech Notes\u003c\/span\u003e\u003c\/h4\u003e\n\u003cp\u003e\u003cb\u003eOne-step purification using \u003c\/b\u003e\u003cb\u003eP\u003c\/b\u003e\u003cb\u003eroteIndex\u003c\/b\u003e\u003cb\u003e™ \u003c\/b\u003e\u003cb\u003eHiBond\u003c\/b\u003e\u003cb\u003e™ Ni-NTA \u003c\/b\u003e\u003cb\u003eAgarose 6 Fast Flow resins \u003c\/b\u003e\u003c\/p\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 0px; border-color: #ffffff;\" width=\"30%\"\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Ni-NTA_2.png?v=1496932129\" alt=\"\"\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 0px; border-color: #ffffff;\" width=\"30%\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cstrong\u003eFigure 1. \u003c\/strong\u003eE.coli lysate containing a 50 kDa target protein was directly loaded on HiBond™ Ni-NTA Agarose 6 FF column. The column was then washed sequentially with 0 mM, 5 mM, and 20 mM imidazole solution before elution with 300 mM imidazole. The target protein was analyzed by SDS-PAGE. \u003cb\u003eC\u003c\/b\u003e\u003cb\u003eL\u003c\/b\u003e: raw lysate; \u003cb\u003eFT\u003c\/b\u003e: unbound flow-through; \u003cb\u003eW1\u003c\/b\u003e,\u003cb\u003e W2 \u003c\/b\u003eand \u003cb\u003eW3: \u003c\/b\u003ewashes; \u003cb\u003eE1\u003c\/b\u003e and \u003cb\u003eE2\u003c\/b\u003e: eluate fractions.\n\u003cul\u003e\u003c\/ul\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"200\"\u003e\u003cstrong\u003eLigand\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eNickel\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBinding capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;40 mg 6xHis-tagged protein\/mL medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMatrix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e4% agarose supplied as 50% slurry\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eParticle size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e45 μm - 165 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaximum pressure\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.1 MPa (1 bar)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eRecommended flow rate\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003eGravity flow, \u0026lt;75 cm\/hour\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eChemical compatibility\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003ctable width=\"93%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"37%\" valign=\"top\"\u003eReducing agents\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e5 mM DTE\u003cbr\u003e0.5-1mM DTT\u003cbr\u003e20 mM β-mercaptoethanol\u003cbr\u003e5 mM TCEP\u003cbr\u003e10 mM reduced glutathione (GSH)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"37%\" valign=\"top\"\u003eDenaturing agents\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e8 M urea, 6 M Gua-HCl \u003cbr\u003e(Tested for one week at 40°C)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"37%\" valign=\"top\"\u003eDetergents\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e2% Triton™ X-100 (nonionic)\u003cbr\u003e2% Tween™ 20 (nonionic)\u003cbr\u003e2% NP-40 (nonionic)\u003cbr\u003e2% cholate (anionic)\u003cbr\u003e1% CHAPS (zwitterionic)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"37%\" valign=\"top\"\u003eOther additives\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e500 mM imidazole\u003cbr\u003e20% ethanol\u003cbr\u003e50% glycerol\u003cbr\u003e100 mM Na\u003csub\u003e2\u003c\/sub\u003eSO4\u003cbr\u003e1.5 M NaCl\u003cbr\u003e1 mM EDTA\u003cbr\u003e60 mM citrate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"37%\" valign=\"top\"\u003eCommonly used buffer\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e50 mM sodium phosphate, pH 7.4\u003cbr\u003e100 mM Tris-HCl, pH 7.4\u003cbr\u003e100 mM Tris-acetate, pH 7.4\u003cbr\u003e100 mM HEPES, pH 7.4\u003cbr\u003e100 mM MOPS, pH 7.4\u003cbr\u003e100 mM sodium acetate, pH 4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage buffer\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1x PBS containing 20% ethanol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProduct is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e   \u003cimg alt=\"Keep refrigerated\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Keep-refrigerated_95fa1867-1f0d-47e6-823a-8a0008076e21_small.png?12332456762384261517\" style=\"float: none;\"\u003e   \u003cimg alt=\"Do not freeze\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Do-not-freeze_2_small.png?12332456762384261517\" style=\"float: none;\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003e\u003c\/h5\u003e\n\u003ch4 style=\"color: #ee8012;\"\u003e\u003c\/h4\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"10 mL","offer_id":18927593668,"sku":"11-0224-010","price":115.0,"currency_code":"USD","in_stock":true},{"title":"50 mL","offer_id":18927593732,"sku":"11-0224-050","price":412.0,"currency_code":"USD","in_stock":true},{"title":"100 mL","offer_id":18927593796,"sku":"11-0224-100","price":759.0,"currency_code":"USD","in_stock":true},{"title":"500 mL","offer_id":39291740979243,"sku":"11-0224-500","price":2733.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/11-0224_Ni-NTA.png?v=1483498777"},{"product_id":"proteindex-ni-nta-agarose-6ff","title":"PROTEINDEX™ HiBond™ Ni-NTA Agarose 6FF","description":"\u003cp\u003e\u003cstrong\u003ePROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e\u003c\/strong\u003e™ \u003cb\u003eHiBond\u003c\/b\u003e\u003cb\u003e™ \u003c\/b\u003e\u003cstrong\u003eNi-NTA Agarose\u003c\/strong\u003e \u003cstrong\u003e6FF\u003c\/strong\u003e has been optimized for automated purification applications. It has been widely used in scale-up and preparative purification of histidine-tagged proteins that are expressed in various hosts, such as \u003cem\u003eE.coli\u003c\/em\u003e, yeast, insect cells, and mammalian cells.\u003c\/p\u003e\n\u003cp\u003e        The base matrix of PROTEINDEX™ HiBond™ Ni-NTA Agarose 6FF is highly crosslinked 6% agarose, offering excellent chemical-, and mechanical stability for automated applications. The matrix support is functionalized with the chelate ligand Nitilotriacetic Acid (NTA) in complex with the nickel ions (Ni\u003csup\u003e2+\u003c\/sup\u003e) in a very stable octahedral structure. The structure of Ni-NTA complex is compatible solutions that contain certain concentration of reducing agents, denaturing agents, detergents and other additives.\u003c\/p\u003e\n\u003cp\u003e        The NTA ligand coordinates the Ni\u003csup\u003e2+\u003c\/sup\u003e with four valencies (tetradentate, coordination number 4), and two valencies are available for interaction with imidazole rings of histidine residues. The number of available valencies seems to provide the best combination of binding effectiveness and chemical stability for binding with the His tag. The Ni-NTA ion-chelate complex on our ProteIndex™ HiBond™ Ni-NTA resins has been delicately optimized to ensure the most effective capture for His tag purification of His-tagged proteins. \u003c\/p\u003e\n\u003cp\u003e       \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eProduct Highlights\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eMechanical stable, designed for scaling up His tag purification\u003c\/li\u003e\n\u003cli\u003eHigh yield (typically \u0026gt; 40 mg protein \/mL resins)\u003c\/li\u003e\n\u003cli\u003eLow leakage of nickel ions. Can be re-used for up to 8 preps*\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cspan style=\"color: #666666;\"\u003e*Note: No significant decrease in yield after 8 repetitive uses of resin when recommended buffers are used. Refer to Product Details for compatible reagents.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eApplications\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eAutomated or syringe operations; and manual batch or gravity flow\u003c\/li\u003e\n\u003cli\u003eRoutine His tag protein purification for structural biology and functional assays\u003c\/li\u003e\n\u003cli\u003eScaling up and preparative applications\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eTech Notes\u003c\/span\u003e\u003c\/h4\u003e\n\u003cp\u003e\u003cb\u003eOne-step purification using \u003c\/b\u003e\u003cb\u003eP\u003c\/b\u003e\u003cb\u003eroteIndex\u003c\/b\u003e\u003cb\u003e™ \u003c\/b\u003e\u003cb\u003eHiBond\u003c\/b\u003e\u003cb\u003e™ Ni-NTA \u003c\/b\u003e\u003cb\u003eAgarose 6 Fast Flow resins \u003c\/b\u003e\u003c\/p\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 0px; border-color: #ffffff;\" width=\"35%\"\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Ni-NTA_6FF_2.png?v=1496201543\" alt=\"\" style=\"display: block; margin-left: auto; margin-right: auto;\"\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 0px; border-color: #ffffff;\" width=\"35%\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cstrong\u003eFigure 1.\u003c\/strong\u003e A 15 kDa protein was expressed in E.coli and purified on an automated system using \u003cb\u003eP\u003c\/b\u003e\u003cb\u003eroteIndex\u003c\/b\u003e\u003cb\u003e™ \u003c\/b\u003e\u003cb\u003eHiBond\u003c\/b\u003e\u003cb\u003e™ Ni-NTA \u003c\/b\u003e\u003cb\u003eAgarose 6 Fast Flow resin \u003c\/b\u003eunder native conditions. 50 mL E.coli lysate in 50 mM NaH\u003csub\u003e2\u003c\/sub\u003ePO\u003csub\u003e4\u003c\/sub\u003e, 0.3 M NaCl, 10 mM imidazole was loaded on the column packed with 0.5 mL resins at the speed of 1 mL\/min. The column was then washed with 100 mL 50 mM NaH\u003csub\u003e2\u003c\/sub\u003ePO\u003csub\u003e4\u003c\/sub\u003e, 0.3 M NaCl, 20 mM imidazole at the speed of 1~2 mL\/min. The target protein was eluted with 50 mM NaH\u003csub\u003e2\u003c\/sub\u003ePO\u003csub\u003e4\u003c\/sub\u003e, 0.3 M NaCl, 250 mM imidazole. Proteins were visualized by coomassie blue staining after separation on SDS-PAGE. \u003cb\u003eL\u003c\/b\u003e: raw lysate; \u003cb\u003eF\u003c\/b\u003e: unbound flow-through; \u003cb\u003eE1\u003c\/b\u003e and \u003cb\u003eE2\u003c\/b\u003e: eluate fractions.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 0px; border-color: #ffffff;\" width=\"37%\"\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Ni-NTA_6FF_comparison_with_Ni_sepharose_4.png?v=1497317795\" alt=\"\"\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 0px; border-color: #ffffff;\" width=\"37%\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cstrong\u003eFigure 2.\u003c\/strong\u003e A 25 kDa protein was expressed in E.coli and purified on an automated system using a one-step automated protocol with \u003cb\u003eP\u003c\/b\u003e\u003cb\u003eroteIndex\u003c\/b\u003e\u003cb\u003e™ \u003c\/b\u003e\u003cb\u003eHiBond\u003c\/b\u003e\u003cb\u003e™ Ni-NTA \u003c\/b\u003e\u003cb\u003eAgarose 6 Fast Flow resin \u003c\/b\u003eunder native conditions. 50 mL E.coli lysate in 50 mM NaH\u003csub\u003e2\u003c\/sub\u003ePO\u003csub\u003e4\u003c\/sub\u003e, 0.3 M NaCl, 10 mM imidazole was loaded on the column packed with 0.5 mL resins at the speed of 1 mL\/min. The column was then washed with 100 mL 50 mM NaH\u003csub\u003e2\u003c\/sub\u003ePO\u003csub\u003e4\u003c\/sub\u003e, 0.3 M NaCl, 20 mM imidazole at the speed of 1~2 mL\/min. The target protein was eluted with 50 mM NaH\u003csub\u003e2\u003c\/sub\u003ePO\u003csub\u003e4\u003c\/sub\u003e, 0.3 M NaCl, 250 mM imidazole. A fraction of eluate was analyzed on a SDS-PAGE. Shown were coomassie blue stains of the target proteins purified from columns of Marvelgent’s ProteIndex Ni-NTA 6FF (\u003cstrong\u003eM\u003c\/strong\u003e) and Ni-Sepharose (\u003cstrong\u003eG\u003c\/strong\u003e) resins.\u003cbr\u003e\u003c\/p\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"200\"\u003e\u003cstrong\u003eLigand\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eNickel\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBinding capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u0026gt; 40 mg 6xHis-tagged protein\/mL medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMatrix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e6% highly cross-linked agarose supplied as 50% slurry\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eParticle size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e45 μm - 165 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaximum pressure\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.3 MPa (3 bar)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eChemical compatibility\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003ctable width=\"93%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"37%\" valign=\"top\"\u003eReducing agents\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e5 mM DTE\u003cbr\u003e0.5-1 mM DTT\u003cbr\u003e20 mM β-mercaptoethanol\u003cbr\u003e5 mM TCEP\u003cbr\u003e10 mM reduced glutathione (GSH)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"37%\" valign=\"top\"\u003eDenaturing agents\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e8 M urea, 6 M Gua-HCl \u003cbr\u003e(Tested for one week at 40°C)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"37%\" valign=\"top\"\u003eDetergents\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e2% Triton™ X-100 (nonionic)\u003cbr\u003e2% Tween™ 20 (nonionic)\u003cbr\u003e2% NP-40 (nonionic)\u003cbr\u003e2% cholate (anionic)\u003cbr\u003e1% CHAPS (zwitterionic)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"37%\" valign=\"top\"\u003eOther additives\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e500 mM imidazole\u003cbr\u003e20% ethanol\u003cbr\u003e50% glycerol\u003cbr\u003e100 mM Na\u003csub\u003e2\u003c\/sub\u003eSO4\u003cbr\u003e1.5 M NaCl\u003cbr\u003e1 mM EDTA\u003cbr\u003e60 mM citrate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"37%\" valign=\"top\"\u003eCommonly used buffer\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e50 mM sodium phosphate, pH 7.4\u003cbr\u003e100 mM Tris-HCl, pH 7.4\u003cbr\u003e100 mM Tris-acetate, pH 7.4\u003cbr\u003e100 mM HEPES, pH 7.4\u003cbr\u003e100 mM MOPS, pH 7.4\u003cbr\u003e100 mM sodium acetate, pH 4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage buffer\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1x PBS containing 20% ethanol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProduct is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e   \u003cimg alt=\"Keep refrigerated\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Keep-refrigerated_95fa1867-1f0d-47e6-823a-8a0008076e21_small.png?12332456762384261517\" style=\"float: none;\"\u003e   \u003cimg alt=\"Do not freeze\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Do-not-freeze_2_small.png?12332456762384261517\" style=\"float: none;\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003e\u003cbr\u003e\u003c\/h5\u003e\n\u003ch4 style=\"color: #ee8012;\"\u003e\u003cbr\u003e\u003c\/h4\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"10 mL","offer_id":19000297540,"sku":"11-0225-010","price":126.0,"currency_code":"USD","in_stock":true},{"title":"50 mL","offer_id":19000297604,"sku":"11-0225-050","price":506.0,"currency_code":"USD","in_stock":true},{"title":"100 mL","offer_id":19000297668,"sku":"11-0225-100","price":825.0,"currency_code":"USD","in_stock":true},{"title":"500 mL","offer_id":5704109129767,"sku":"11-0225-500","price":3366.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/11-0225_Ni-NTA_6FF.png?v=1496933028"},{"product_id":"proteindex-ni-nta-agarose-6ff-prepacked-cartridge","title":"PROTEINDEX™ HiBond™ Ni-NTA Agarose 6FF, Prepacked Cartridge","description":"\u003cp\u003e\u003cstrong\u003ePROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e\u003c\/strong\u003e™ \u003cb\u003eHiBond\u003c\/b\u003e\u003cb\u003e™ \u003c\/b\u003e\u003cstrong\u003eNi-NTA Agarose\u003c\/strong\u003e \u003cstrong\u003e6FF\u003c\/strong\u003e has been optimized for automated purification applications. It has been widely used in scale-up and preparative purification of histidine-tagged proteins that are expressed in various hosts, such as \u003cem\u003eE.coli\u003c\/em\u003e, yeast, insect cells, and mammalian cells.\u003c\/p\u003e\n\u003cp\u003e        The base matrix of PROTEINDEX™ HiBond™ Ni-NTA Agarose 6FF is highly crosslinked 6% agarose, offering excellent chemical-, and mechanical stability for automated applications. The matrix support is functionalized with the chelate ligand Nitilotriacetic Acid (NTA) in complex with the nickel ions (Ni\u003csup\u003e2+\u003c\/sup\u003e) in a very stable octahedral structure. The structure of Ni-NTA complex is compatible solutions that contain certain concentration of reducing agents, denaturing agents, detergents and other additives.\u003c\/p\u003e\n\u003cp\u003e        The NTA ligand coordinates the Ni\u003csup\u003e2+\u003c\/sup\u003e with four valencies (tetradentate, coordination number 4), and two valencies are available for interaction with imidazole rings of histidine residues. The number of available valencies seems to provide the best combination of binding effectiveness and chemical stability for binding with the His tag. The Ni-NTA ion-chelate complex on our ProteIndex™ HiBond™ Ni-NTA resins has been delicately optimized to ensure the most effective capture for His tag purification of His-tagged proteins. \u003c\/p\u003e\n\u003cp\u003e        The Prepacked Cartridge has standard interface and can be adapted to most commonly used chromatography systems, such as ÄKTA systems.\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e        Value bundle with a \u003c\/span\u003e\u003ca rel=\"noopener\" title=\"Connector Luer Female \/ 10-32 Male in PEEK\" href=\"https:\/\/marvelgent.com\/products\/connector-1-16-luer-female-10-32-male-peek\" target=\"_blank\"\u003eLuer Female\/10-32 Male Connector in PEEK\u003c\/a\u003e\u003cspan\u003e (Cat# 20-0315) is now available for all pack sizes, providing convenient operation of the pre-packed column with a syringe at an excellent value.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eProduct Highlights\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eConvenient, easy, ready-to-use\u003c\/li\u003e\n\u003cli\u003eMechanical stable, designed for large scale purification of histidine-tagged proteins\u003c\/li\u003e\n\u003cli\u003eHigh yield (typically \u0026gt; 40 mg protein \/mL resins)\u003c\/li\u003e\n\u003cli\u003eLow leakage of nickel ions. Can be re-used for up to 8 preps*\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cspan style=\"color: #666666;\"\u003e*Note: No significant decrease in yield after 8 repetitive uses of resin when recommended buffers are used. Refer to Product Details for compatible reagents.\u003c\/span\u003e\u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eApplications\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eAutomated purifications\u003c\/li\u003e\n\u003cli\u003eRoutine His tag protein purification for structural biology and functional assays\u003c\/li\u003e\n\u003cli\u003eScaling up and preparative applications\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eTech Notes\u003c\/span\u003e\u003c\/h4\u003e\n\u003cp\u003e\u003cb\u003eOne-step purification using \u003c\/b\u003e\u003cb\u003eP\u003c\/b\u003e\u003cb\u003eroteIndex\u003c\/b\u003e\u003cb\u003e™ \u003c\/b\u003e\u003cb\u003eHiBond\u003c\/b\u003e\u003cb\u003e™ Ni-NTA \u003c\/b\u003e\u003cb\u003eAgarose 6 Fast Flow resins \u003c\/b\u003e\u003c\/p\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"35%\" style=\"border: 0px; border-color: #ffffff;\"\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Ni-NTA_6FF_2.png?v=1496201543\"\u003e\u003c\/td\u003e\n\u003ctd width=\"35%\" style=\"border: 0px; border-color: #ffffff;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cstrong\u003eFigure 1.\u003c\/strong\u003e A 15 kDa protein was expressed in E.coli and purified on an automated system using \u003cb\u003eP\u003c\/b\u003e\u003cb\u003eroteIndex\u003c\/b\u003e\u003cb\u003e™ \u003c\/b\u003e\u003cb\u003eHiBond\u003c\/b\u003e\u003cb\u003e™ Ni-NTA \u003c\/b\u003e\u003cb\u003eAgarose 6 Fast Flow resin \u003c\/b\u003eunder native conditions. 50 mL E.coli lysate in 50 mM NaH\u003csub\u003e2\u003c\/sub\u003ePO\u003csub\u003e4\u003c\/sub\u003e, 0.3 M NaCl, 10 mM imidazole was loaded on the column packed with 0.5 mL resins at the speed of 1 mL\/min. The column was then washed with 100 mL 50 mM NaH\u003csub\u003e2\u003c\/sub\u003ePO\u003csub\u003e4\u003c\/sub\u003e, 0.3 M NaCl, 20 mM imidazole at the speed of 1~2 mL\/min. The target protein was eluted with 50 mM NaH\u003csub\u003e2\u003c\/sub\u003ePO\u003csub\u003e4\u003c\/sub\u003e, 0.3 M NaCl, 250 mM imidazole. Proteins were visualized by coomassie blue staining after separation on SDS-PAGE. \u003cb\u003eL\u003c\/b\u003e: raw lysate; \u003cb\u003eF\u003c\/b\u003e: unbound flow-through; \u003cb\u003eE1\u003c\/b\u003e and \u003cb\u003eE2\u003c\/b\u003e: eluate fractions.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"37%\" style=\"border: 0px; border-color: #ffffff;\"\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Ni-NTA_6FF_comparison_with_Ni_sepharose_4.png?v=1497317795\"\u003e\u003c\/td\u003e\n\u003ctd width=\"37%\" style=\"border: 0px; border-color: #ffffff;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cstrong\u003eFigure 2.\u003c\/strong\u003e A 25 kDa protein was expressed in E.coli and purified on an automated system using a one-step automated protocol with \u003cb\u003eP\u003c\/b\u003e\u003cb\u003eroteIndex\u003c\/b\u003e\u003cb\u003e™ \u003c\/b\u003e\u003cb\u003eHiBond\u003c\/b\u003e\u003cb\u003e™ Ni-NTA \u003c\/b\u003e\u003cb\u003eAgarose 6 Fast Flow resin \u003c\/b\u003eunder native conditions. 50 mL E.coli lysate in 50 mM NaH\u003csub\u003e2\u003c\/sub\u003ePO\u003csub\u003e4\u003c\/sub\u003e, 0.3 M NaCl, 10 mM imidazole was loaded on the column packed with 0.5 mL resins at the speed of 1 mL\/min. The column was then washed with 100 mL 50 mM NaH\u003csub\u003e2\u003c\/sub\u003ePO\u003csub\u003e4\u003c\/sub\u003e, 0.3 M NaCl, 20 mM imidazole at the speed of 1~2 mL\/min. The target protein was eluted with 50 mM NaH\u003csub\u003e2\u003c\/sub\u003ePO\u003csub\u003e4\u003c\/sub\u003e, 0.3 M NaCl, 250 mM imidazole. A fraction of eluate was analyzed on a SDS-PAGE. Shown were coomassie blue stains of the target proteins purified from columns of Marvelgent’s ProteIndex Ni-NTA 6FF (\u003cstrong\u003eM\u003c\/strong\u003e) and Ni-Sepharose (\u003cstrong\u003eG\u003c\/strong\u003e) resins.\u003c\/p\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"200\"\u003e\u003cstrong\u003eLigand\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eNickel\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBinding capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;40 mg 6xHis-tagged protein\/mL medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMatrix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e6% highly cross-linked agarose\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eParticle size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e45 μm - 165 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eColumn volume\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1 mL or 5 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaximum pressure\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.3 MPa (3 bar)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eChemical compatibility\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003ctable width=\"93%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"37%\"\u003eReducing agents\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e5 mM DTE\u003cbr\u003e0.5-1 mM DTT\u003cbr\u003e20 mM β-mercaptoethanol\u003cbr\u003e5 mM TCEP\u003cbr\u003e10 mM reduced glutathione (GSH)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"37%\"\u003eDenaturing agents\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e8 M urea, 6 M Gua-HCl \u003cbr\u003e(Tested for one week at 40°C)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"37%\"\u003eDetergents\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e2% Triton™ X-100 (nonionic)\u003cbr\u003e2% Tween™ 20 (nonionic)\u003cbr\u003e2% NP-40 (nonionic)\u003cbr\u003e2% cholate (anionic)\u003cbr\u003e1% CHAPS (zwitterionic)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"37%\"\u003eOther additives\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e500 mM imidazole\u003cbr\u003e20% ethanol\u003cbr\u003e50% glycerol\u003cbr\u003e100 mM Na\u003csub\u003e2\u003c\/sub\u003eSO4\u003cbr\u003e1.5 M NaCl\u003cbr\u003e1 mM EDTA\u003cbr\u003e60 mM citrate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"37%\"\u003eCommonly used buffer\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e50 mM sodium phosphate, pH 7.4\u003cbr\u003e100 mM Tris-HCl, pH 7.4\u003cbr\u003e100 mM Tris-acetate, pH 7.4\u003cbr\u003e100 mM HEPES, pH 7.4\u003cbr\u003e100 mM MOPS, pH 7.4\u003cbr\u003e100 mM sodium acetate, pH 4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eColumn hardware pressure limit\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003e5 bar (0.5 MPa)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage buffer\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1x PBS containing 20% ethanol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProduct is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e   \u003cimg style=\"float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Keep-refrigerated_95fa1867-1f0d-47e6-823a-8a0008076e21_small.png?12332456762384261517\" alt=\"Keep refrigerated\"\u003e   \u003cimg style=\"float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Do-not-freeze_2_small.png?12332456762384261517\" alt=\"Do not freeze\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"5x 1 mL Columns","offer_id":19098127684,"sku":"11-0230-5x1ML","price":172.0,"currency_code":"USD","in_stock":true},{"title":"5x 1 mL Columns with 1 Connector","offer_id":41405616259115,"sku":"11-0230-5x1MLB","price":200.0,"currency_code":"USD","in_stock":true},{"title":"1x 5 mL Column","offer_id":19098127748,"sku":"11-0230-1x5ML","price":149.0,"currency_code":"USD","in_stock":true},{"title":"1x 5 mL Column with 1 Connector","offer_id":41405616291883,"sku":"11-0230-1x5MLB","price":177.0,"currency_code":"USD","in_stock":true},{"title":"5x 5mL Columns","offer_id":19098127812,"sku":"11-0230-5x5ML","price":539.0,"currency_code":"USD","in_stock":true},{"title":"5x 5 mL Columns with 1 Connector","offer_id":41405616324651,"sku":"11-0230-5x5MLB","price":566.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/Ni-NTA_6FF_prepacked_columns.png?v=1496933949"},{"product_id":"proteindex-co-nta-agarose-6ff-prepacked-cartridge","title":"PROTEINDEX™ PureBind™ Co-NTA Agarose 6 Fast Flow, Prepacked Cartridge","description":"\u003cp\u003e\u003cstrong\u003eROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e\u003c\/strong\u003e™\u003cstrong\u003e\u003cstrong\u003e\u003c\/strong\u003e Co-NTA Agarose 6FF\u003c\/strong\u003e is a  cobalt-based immobilized metal ion affinity chromatography (IMAC) medium. It offers greater specificity and therefore enhanced selectivity for polyhistidine-tagged proteins under both native and denaturing conditions, compared to Nickel-charged media. Co-NTA Agarose 6 Fast Flow resins can be used for small-scale and preparative large-scale purification of histidine-tagged recombinant proteins from prokaryotic and eukaryotic expression systems.\u003c\/p\u003e\n\u003cp\u003eThe Prepacked Cartridge has standard interface and can be adapted to most commonly used chromatography systems, such as ÄKTA systems.\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eValue bundle with a \u003c\/span\u003e\u003ca rel=\"noopener\" title=\"Connector Luer Female \/ 10-32 Male in PEEK\" href=\"https:\/\/marvelgent.com\/products\/connector-1-16-luer-female-10-32-male-peek\" target=\"_blank\"\u003eLuer Female\/10-32 Male Connector in PEEK\u003c\/a\u003e\u003cspan\u003e (Cat# 20-0315) is now available for all pack sizes, providing convenient operation of the pre-packed column with a syringe at an excellent value.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eProduct Highlights\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cstrong\u003eReady-to-use, convenient formats: 1 mL and 5 mL\u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cstrong\u003eMechanical stable, suited for preparative and large scale purification of histidine-tagged proteins\u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cstrong\u003eHigher specificity and selectivity for His-tagged proteins, compared with Nickel-based IMAC. Can be used to purify challenging proteins that are expressed in low levels or when the use of nickel is not desired.\u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cstrong\u003eSuitable for purifying proteins from prokaryotic and eukaryotic expression systems\u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cstrong\u003eCompatible with applications under native and denaturing conditions \u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cstrong\u003eLow cobalt ion leakage.\u003c\/strong\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"200\"\u003e\u003cstrong\u003eLigand\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eCobalt ion\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBinding capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;20 mg 6XHis-tagged protein\/mL medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMatrix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eHighly cross-linked 6% agarose resins\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eParticle size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e45 μm - 165 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaximum pressure\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.3 MPa (3 bar)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eChemical compatibility\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003ctable width=\"93%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"37%\" valign=\"top\"\u003eReducing agents\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e10 mM β-mercaptoethanol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"37%\" valign=\"top\"\u003eDenaturing agents\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e8 M urea, 6 M Gua-HCl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"37%\" valign=\"top\"\u003eDetergents\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e\u0026lt;1% Triton™ X-100 (nonionic)\u003cbr\u003e1% NP-40 (nonionic)\u003cbr\u003e1% CHAPS (zwitterionic)\u003cbr\u003e1% SDS\u003cbr\u003e1% sarcosyl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"37%\" valign=\"top\"\u003eOther additives\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e≤500 mM imidazoleat pH7.0 ~ 8.0 for elution\u003cbr\u003e30% ethanol\u003cbr\u003e20% glycerol\u003cbr\u003e500 mM KCl\u003cbr\u003e1.0 M NaCl\u003cbr\u003e20 mM MES\u003cbr\u003e50 mM Tris\u003cbr\u003e50 mM HEPES\u003cbr\u003e60 mM MOPS\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage buffer\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1X PBS containing 20% ethanol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProduct is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e   \u003cimg alt=\"Keep refrigerated\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Keep-refrigerated_95fa1867-1f0d-47e6-823a-8a0008076e21_small.png?12332456762384261517\" style=\"float: none;\"\u003e   \u003cimg alt=\"Do not freeze\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Do-not-freeze_2_small.png?12332456762384261517\" style=\"float: none;\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"5x 1 mL Columns","offer_id":19255095236,"sku":"11-0253-5x1ML","price":181.0,"currency_code":"USD","in_stock":true},{"title":"5x 1 mL Columns with 1 Connector","offer_id":41405694279723,"sku":"11-0253-5x1MLB","price":209.0,"currency_code":"USD","in_stock":true},{"title":"1x 5 mL Column","offer_id":19255095300,"sku":"11-0253-1x5ML","price":152.0,"currency_code":"USD","in_stock":true},{"title":"1x 5 mL Column with 1 Connector","offer_id":41405694312491,"sku":"11-0253-1x5MLB","price":180.0,"currency_code":"USD","in_stock":true},{"title":"5x 5 mL Columns","offer_id":19255095364,"sku":"11-0253-5x5ML","price":592.0,"currency_code":"USD","in_stock":true},{"title":"5x 5 mL Columns with 1 Connector","offer_id":41405694345259,"sku":"11-0253-5x5MLB","price":620.0,"currency_code":"USD","in_stock":true},{"title":"16\/10 Column, 20 mL","offer_id":42061409845291,"sku":"11-0253-C20","price":629.0,"currency_code":"USD","in_stock":true},{"title":"7\/10 Columns, 4.7 mL, Pack of 2","offer_id":42061614022699,"sku":"11-0253-C47","price":478.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/Co-NTA_6FF_prepacked_columns.png?v=1483557966"},{"product_id":"empty-gravity-flow-columns-12-ml","title":"Empty Disposable Gravity Flow Columns, Fritted, 12 mL, with 20 µm frits","description":"\u003cp\u003eThese disposable columns are economical, versatile columns for single-use purification applications using gravity flow.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eSimple, cost-effective, readily adaptable for a wide range of gravity flow applications\u003c\/li\u003e\n\u003cli\u003eMade of high purity medical grade polypropylene, stable in commonly used chemicals and reagents\u003c\/li\u003e\n\u003cli\u003eAvailable in various sizes to suit for different experiments\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eIn the complete set, each fritted column comes with one top plug and one end cap. An extra filter disc is also provided and can be used as upper bed support. Placing a filter disc on top of the gravity-settled medium bed helps prevent drying of the medium.\u003c\/p\u003e\n\u003cp\u003eThe fritted column can be purchased separately as the SE version, providing a more cost-effective option for applications where no column caps nor extra frits are needed.\u003c\/p\u003e\n\u003ch4\u003e \u003c\/h4\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eProduct Details\u003c\/span\u003e\u003c\/h4\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"190\"\u003e\u003cstrong\u003eMaximum capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e12 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBed volume\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0 - 10 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eColumn inner diameter\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e15.6 mm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eColumn height\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e83 mm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaterial (column)\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003ePolypropylene (PP)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaterial (filter disc)\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003ePolyethylene (PE)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eFilter pore size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e20 µm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1 empty column\u003c\/p\u003e\n\u003cp\u003e1 top plug\u003c\/p\u003e\n\u003cp\u003e1 end cap\u003c\/p\u003e\n\u003cp\u003e2 filter discs (one is inserted and the other is provided as optional extra)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eChemical stability\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStable in all commonly used reagents\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"190\" valign=\"top\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFor fast, general-purpose purification by gravity flow or low pressure:\n\u003cul style=\"padding-left: 60px;\"\u003e\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003eAffinity chromatography of protein or other macromolecules\u003c\/li\u003e\n\u003cli\u003eImmunodepletion of immune reactive complexes\u003c\/li\u003e\n\u003cli\u003eSize-exclusion chromatography\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul style=\"padding-left: 60px;\"\u003e\u003c\/ul\u003e\n\u003cstrong\u003e\u003cem\u003eFor research use only. Not intended for any human or animal therapeutic or diagnostic uses.\u003c\/em\u003e\u003c\/strong\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eWe provide a variety of columns to fit your application needs. \u003ca href=\"https:\/\/marvelgent.com\/collections\/empty-disposable-gravity-flow-columns#top\" title=\"Empty Disposable Gravity Flow Columns\"\u003eView our collection and selection disposable gravity columns for your application \u0026gt;\u0026gt;\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"Pack of 50","offer_id":20503530244,"sku":"11-0257-050","price":147.0,"currency_code":"USD","in_stock":true},{"title":"Pack of 100","offer_id":20503530308,"sku":"11-0257-100","price":259.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/12_ml_disposable_columns_1.png?v=1543442209"},{"product_id":"proteindex-co-nta-agarose","title":"PROTEINDEX™ PureBind™ Co-NTA Agarose","description":"\u003cp\u003e\u003cstrong\u003ePROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e\u003c\/strong\u003e™\u003cstrong\u003e\u003cstrong\u003e\u003c\/strong\u003e PureBind™ Co-NTA Agarose \u003c\/strong\u003eis a  cobalt-based immobilized metal ion affinity chromatography (IMAC) medium. It offers greater specificity and therefore enhanced selectivity for polyhistidine-tagged proteins under both native and denaturing conditions, compared to Ni-NTA media.     \u003c\/p\u003e\n\u003cp\u003eCo-NTA Agarose resins can be used for batch\/gravity flow purification of polyhistidine-tagged recombinant proteins.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eProduct Highlights\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cstrong\u003eSuited for batch or gravity flow purification of histidine-tagged proteins\u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cstrong\u003eHigher specificity and selectivity for His-tagged proteins, compared with Ni-NTA IMAC. Can be used to purify challenging proteins that are expressed in low levels or when the use of nickel is not desired. \u003cbr\u003e\u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cstrong\u003eSuitable for purifying proteins from prokaryotic and eukaryotic expression systems\u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cstrong\u003eCompatible with applications under native and denaturing conditions \u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cstrong\u003eLow cobalt ion leakage.\u003c\/strong\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"200\"\u003e\u003cstrong\u003eLigand\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eCobalt ion\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBinding capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;20 mg 6XHis-tagged protein\/mL medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMatrix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e4% agarose resins supplied as 50% slurry\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eParticle size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e45 μm - 165 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaximum pressure\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.1 MPa (1 bar)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eChemical compatibility\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003ctable width=\"93%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"37%\"\u003eReducing agents\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e10 mM β-mercaptoethanol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"37%\"\u003eDenaturing agents\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e8 M urea, 6 M Gua-HCl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"37%\"\u003eDetergents\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e\u0026lt;1% Triton™ X-100 (nonionic)\u003cbr\u003e1% NP-40 (nonionic)\u003cbr\u003e1% CHAPS (zwitterionic)\u003cbr\u003e1% SDS\u003cbr\u003e1% sarcosyl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"37%\"\u003eOther additives\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e≤500 mM imidazole at pH7.0 ~ 8.0 for elution\u003cbr\u003e30% ethanol\u003cbr\u003e20% glycerol\u003cbr\u003e500 mM KCl\u003cbr\u003e1.0 M NaCl\u003cbr\u003e20 mM MES\u003cbr\u003e50 mM Tris\u003cbr\u003e50 mM HEPES\u003cbr\u003e60 mM MOPS\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage buffer\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1X PBS containing 20% ethanol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProduct is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e   \u003cimg style=\"float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Keep-refrigerated_95fa1867-1f0d-47e6-823a-8a0008076e21_small.png?12332456762384261517\" alt=\"Keep refrigerated\"\u003e   \u003cimg style=\"float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Do-not-freeze_2_small.png?12332456762384261517\" alt=\"Do not freeze\"\u003e\u003c\/p\u003e\n\u003ch5\u003e\u003cbr\u003e\u003c\/h5\u003e\n\u003ch5\u003eDocuments\u003c\/h5\u003e\n\u003ch4\u003eProduct Information Sheet\u003c\/h4\u003e\n\u003cp\u003e🗎    \u003ca rel=\"noopener noreferrer\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Man_11-0252_Co-NTA_Agarose_v1605.pdf?16279790217106739219\" title=\"Man_11-0252_Co-NTA_Agarose_v1605.pdf\" target=\"_blank\"\u003eCobalt NTA Agarose \u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003e\u003cbr\u003e\u003c\/h5\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"10 mL","offer_id":26309510600,"sku":"11-0252-010","price":121.0,"currency_code":"USD","in_stock":true},{"title":"50 mL","offer_id":26309510728,"sku":"11-0252-050","price":498.0,"currency_code":"USD","in_stock":true},{"title":"100 mL","offer_id":26309510792,"sku":"11-0252-100","price":874.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/11-0252_Co-NTA.png?v=1483557922"},{"product_id":"proteindex-ni-ida-agarose-6ff","title":"PROTEINDEX™ Ni-IDA Agarose 6FF","description":"\u003cp\u003e\u003cstrong\u003ePROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e\u003c\/strong\u003e™ \u003cstrong\u003eNi-IDA Agarose\u003c\/strong\u003e \u003cstrong\u003e6FF\u003c\/strong\u003e is designed mainly for scaling up purification of histidine-tagged proteins that are expressed in various hosts, such as \u003cem\u003eE.coli\u003c\/em\u003e, yeast, insect cells, and mammalian cells.\u003c\/p\u003e\n\u003cp\u003eHighly cross-linked 6% agarose as the base matrix support offers excellent mechanical stability for centrifugation, and automated, scale up purification of histidine-tagged proteins.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eProduct Highlights\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cstrong\u003eMechanical stable, designed for high yield, scaling up purification of histidine-tagged proteins\u003cbr\u003e\u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cstrong\u003eHigher binding capacity\u003cbr\u003e\u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cstrong\u003eLow leakage of nickel ions\u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cstrong\u003eVersatile, ready for gravity\/batch, spin, or FPLC \u003cspan data-mce-fragment=\"1\"\u003esystems\u003c\/span\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"200\"\u003e\u003cstrong\u003eLigand\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eNickel\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBinding capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;40 mg 6xHis-tagged protein\/mL medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMatrix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e6% highly cross-linked agarose supplied as 50% slurry\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eParticle size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e45 μm - 165 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaximum pressure\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.3 MPa (3 bar)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage buffer\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1x PBS containing 20% ethanol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProduct is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not freeze.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e   \u003cimg alt=\"Keep refrigerated\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Keep-refrigerated_95fa1867-1f0d-47e6-823a-8a0008076e21_small.png?12332456762384261517\" style=\"float: none;\"\u003e   \u003cimg alt=\"Do not freeze\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Do-not-freeze_2_small.png?12332456762384261517\" style=\"float: none;\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003e\u003cbr\u003e\u003c\/h5\u003e\n\u003ch4 style=\"color: #ee8012;\"\u003e\u003cbr\u003e\u003c\/h4\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"25 mL","offer_id":39321581060139,"sku":"11-0222-025","price":302.0,"currency_code":"USD","in_stock":true},{"title":"50 mL","offer_id":28520239697,"sku":"11-0222-050","price":479.0,"currency_code":"USD","in_stock":true},{"title":"100 mL","offer_id":39321581879339,"sku":"11-0222-100","price":830.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/11-0222Ni-IDA-1.png?v=1694786697"},{"product_id":"proteindex-rprotein-a-agarose-gravity-flow-column","title":"PROTEINDEX™ Recombinant Protein A Prepacked Gravity Flow Columns, 1 mL, Pack of 5 Columns","description":"\u003cp\u003e\u003cstrong\u003ePROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e\u003c\/strong\u003e™ recombinant Protein A Agarose Gravity Flow Column is ready-to-use affinity chromatography column for purification of immunoglobulins, notably IgG isotypes, as well as Fc region-containing fusion proteins.\u003c\/p\u003e\n\u003cp\u003eProtein A, a bacterial cell wall protein isolated from \u003cem\u003eStaphylococcus aureus\u003c\/em\u003e, binds to the Fc region of most immunoglobulins through interactions with the heavy chain. Native Protein A contains five high affinity IgG binding domains and many other domains with unknown functions.\u003c\/p\u003e\n\u003cp\u003eThe recombinant Protein A is a genetically engineered protein that contains only the five high affinity IgG binding domains. The non-essential domains were removed to minimize nonspecific binding. The recombinant Protein A is produced in \u003ci\u003eE. coli, \u003c\/i\u003eand it essentially binds to immunoglobulins with the same degree of affinity as the native Protein A. rProtein A is capable of binding with high affinity to the Fc region from IgG of several species including human and rabbit.\u003c\/p\u003e\n\u003cp\u003eEnabled by proprietary ProteIndex™ technology, immobilization of recombinant Protein A on 4% agarose resins was very stable and was optimized for high yield in laboratory preparations and scale-up applications.\u003c\/p\u003e\n\u003cp\u003eEach gravity column contains 1 mL of recombinant Protein A agarose resin, and is individually wrapped.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eProduct Highlights\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eHigh capacity, \u0026gt; 40 mg human IgG per column\u003c\/li\u003e\n\u003cli\u003eHigh selectivity for high purity\u003c\/li\u003e\n\u003cli\u003eMinimal ligand leakage, can be reused\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"5%\"\u003e\u003c\/td\u003e\n\u003ctd width=\"35%\"\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/ProteinA4FF_purifying_mAscites_fluid_3_0d442212-1ce9-4a05-8106-1ec2848dd3cb.png?v=1508439312\"\u003e\u003c\/td\u003e\n\u003ctd valign=\"bottom\"\u003e\n\u003cp\u003e\u003cstrong\u003eHigh purity and high capacity of recombinant Protein A ligand. \u003c\/strong\u003e2 mL mouse ascites fluid was loaded on the column containing 0.5 mL settled rProtein A 4 FF resin. Bound immunoglobulin species were eluted with 100 mM Glycine, pH 3.0. Fractions were analyzed by SDS-PAGE.\u003c\/p\u003e\n\u003cp\u003eLanes: (1) Raw ascites fluid; (2) Flow-through; (3) Elution. Note that, heavy and light chains of IgG were mostly absent from the flow-through, indicating that most immunoglobulins were bound to the column.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"200\"\u003e\u003cstrong\u003eProduct package size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e5 columns\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"200\"\u003e\u003cstrong\u003eColumn volume\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1 mL settled Protein A resins\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eLigand\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eRecombinant protein A produced in \u003cem\u003eE.coli\u003c\/em\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBinding capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;40 mg rabbit IgG\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMatrix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e4% agarose\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eParticle size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e45 μm - 165 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaximum pressure\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.1 MPa (1 bar)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eChemical stability\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eAll commonly used aqueous buffers, including 6 M guanidine-HCl and 8 M urea.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eRecommended flow rate\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eGravity flow, 100 - 300 cm\/hour\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003epH stability\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eLong term: pH 3 - 9; \u003cbr\u003eShort term: pH 2 - 10\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage buffer\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e20% ethanol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eColumn material\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003epolypropylene (body)\u003cbr\u003eLow density polyethylene (top snap cap and bottom tip cap)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eFrit\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003ePolyethylene, with an average pore size of 30 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProduct is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e   \u003cimg style=\"float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Keep-refrigerated_95fa1867-1f0d-47e6-823a-8a0008076e21_small.png?12332456762384261517\" alt=\"Keep refrigerated\"\u003e   \u003cimg style=\"float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Do-not-freeze_2_small.png?12332456762384261517\" alt=\"Do not freeze\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eDocuments\u003c\/h5\u003e\n\u003ch4\u003eTechnical Note\u003c\/h4\u003e\n\u003cp\u003e🗎    \u003ca rel=\"noopener noreferrer\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Table_Binding_characteristics_of_immunoglobulin-binding_proteins.pdf?6231220717744860621\" title=\"Comparison of binding characteristics of immunoglobulin-binding proteins\" target=\"_blank\"\u003eComparison of Binding Characteristics of Immunoglobulin-Binding Proteins\u003c\/a\u003e\u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"5 Columns","offer_id":5915590983719,"sku":"18-0268","price":247.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/ProteinA_gravity_column.png?v=1512006816"},{"product_id":"empty-disposable-gravity-flow-columns-fritted-12-ml-with-50-um-frits","title":"Empty Disposable Gravity Flow Columns, Fritted, 12 mL, with 50 µm frits","description":"\u003cp\u003eThese disposable columns are economical, versatile columns for single-use purification applications using gravity flow.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eSimple, cost-effective, readily adaptable for a wide range of gravity flow applications\u003c\/li\u003e\n\u003cli\u003eMade of high purity medical grade polypropylene, stable in commonly used chemicals and reagents\u003c\/li\u003e\n\u003cli\u003eAvailable in various sizes to suit for different experiments\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eIn the complete set, each fritted column comes with one top plug and one end cap. An extra filter disc is also provided and can be used as upper bed support. Placing a filter disc on top of the gravity-settled medium bed helps prevent drying of the medium.\u003c\/p\u003e\n\u003cp\u003eThe fritted column can be purchased separately as the SE version, providing a more cost-effective option for applications where no column caps nor extra frits are needed.\u003c\/p\u003e\n\u003ch4\u003e \u003c\/h4\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eProduct Details\u003c\/span\u003e\u003c\/h4\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"190\"\u003e\u003cstrong\u003eMaximum capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e12 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBed volume\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0 - 10 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eColumn inner diameter\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e15.6 mm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eColumn height\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e83 mm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaterial (column)\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003ePolypropylene (PP)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaterial (filter disc)\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003ePolyethylene (PE)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eFilter pore size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e50 µm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1 empty column\u003c\/p\u003e\n\u003cp\u003e1 top plug\u003c\/p\u003e\n\u003cp\u003e1 end cap\u003c\/p\u003e\n\u003cp\u003e2 filter discs (one is inserted and the other is provided as optional extra)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eChemical stability\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStable in all commonly used reagents\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"190\" valign=\"top\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFor fast, general-purpose purification by gravity flow or low pressure:\n\u003cul style=\"padding-left: 60px;\"\u003e\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003eAffinity chromatography of protein or other macromolecules\u003c\/li\u003e\n\u003cli\u003eImmunodepletion of immune reactive complexes\u003c\/li\u003e\n\u003cli\u003eSize-exclusion chromatography\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul style=\"padding-left: 60px;\"\u003e\u003c\/ul\u003e\n\u003cstrong\u003e\u003cem\u003eFor research use only. Not intended for any human or animal therapeutic or diagnostic uses.\u003c\/em\u003e\u003c\/strong\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eWe provide a variety of columns to fit your application needs. \u003ca href=\"https:\/\/marvelgent.com\/collections\/empty-disposable-gravity-flow-columns#top\" title=\"Empty Disposable Gravity Flow Columns\"\u003eView our collection and selection disposable gravity columns for your application \u0026gt;\u0026gt;\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"Pack of 50","offer_id":39629927186475,"sku":"12-0294-050","price":148.0,"currency_code":"USD","in_stock":true},{"title":"Pack of 100","offer_id":39629927219243,"sku":"12-0294-100","price":261.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/12_ml_disposable_columns_1_3f2a0653-6f55-45b1-8166-85284f263673.png?v=1637805104"},{"product_id":"proteindex-alkali-tolerant-protein-a-plus","title":"PROTEINDEX™ Alkali-Tolerant Protein A Plus Agarose Resin, High Capacity","description":"\u003cp\u003e\u003cstrong\u003ePROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e\u003c\/strong\u003e™ rProtein A Plus resin is an agarose-based, proprietary affinity chromatography medium designed for easy, one-step purification of immunoglobulins, notably monoclonal antibodies. \u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eAlkali-tolerant protein A is engineered by gene recombination technology on the base of native protein A.  The Alkali-Tolerant Protein A Plus resin retains ≥95% capacity after 200 times repeated clean-in-place (CIP) cycles with 0.1M NaOH, and approximately 80% of the initial capacity with 0.5M NaOH after 100 times repeated CIP cycles. Alkali-Tolerant Protein A is immobilized onto the resin with an orientation that is optimal for immunoglobulin binding. The level of leakage of the ligand during elution is very low.\u003c\/p\u003e\n\u003cp\u003eThe resin is highly cross-linked agarose based resin. The bead size is smaller and is optimized for higher binding capacity. The matrix is more rigid and offers higher mechanical stability for faster flow rates between 50 - 500 cm\/hour.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 36.6906%;\" width=\"200\"\u003e\u003cstrong\u003eLigand\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 63.1295%;\"\u003eRecombinant alkali-tolerant protein A produced in \u003cem\u003eE.coli\u003c\/em\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 36.6906%;\"\u003e\u003cstrong\u003eBinding capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 63.1295%;\"\u003e\u0026gt;60 mg rabbit IgG\/mL medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 36.6906%;\"\u003e\u003cstrong\u003eMatrix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 63.1295%;\"\u003eHighly cross-linked agarose based resin, supplied as a 50% slurry\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 36.6906%;\"\u003e\u003cstrong\u003eParticle size, d\u003csub\u003e50V\u003c\/sub\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 63.1295%;\"\u003e~ 85 μm \u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 36.6906%;\"\u003e\u003cstrong\u003eMaximum pressure\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 63.1295%;\"\u003e0.3 MPa (3 bar)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 36.6906%;\"\u003e\u003cstrong\u003eRecommended flow rate\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 63.1295%;\"\u003e100 - 500 cm\/hour\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 36.6906%;\" valign=\"top\"\u003e\u003cstrong\u003eChemical stability\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 63.1295%;\"\u003eAll commonly used aqueous buffers and reagents in the process of antibody purification, including 6 M guanidine-HCl and 8 M urea.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 36.6906%;\" valign=\"top\"\u003e\u003cstrong\u003epH Stability\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 63.1295%;\"\u003e\n\u003cp\u003eLong term: pH 3 - 12\u003c\/p\u003e\n\u003cp\u003eShort term: pH 2 - 14\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 36.6906%;\"\u003e\u003cstrong\u003eCleaning-in-place (CIP)\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 63.1295%;\"\u003e0.1 M ~ 0.5 M NaOH\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 36.6906%;\"\u003e\u003cstrong\u003eStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 63.1295%;\"\u003e2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 36.6906%;\"\u003e\u003cstrong\u003eStorage buffer\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 63.1295%;\"\u003e20% ethanol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProduct is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e   \u003cimg style=\"float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Keep-refrigerated_95fa1867-1f0d-47e6-823a-8a0008076e21_small.png?12332456762384261517\" alt=\"Keep refrigerated\"\u003e   \u003cimg style=\"float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Do-not-freeze_2_small.png?12332456762384261517\" alt=\"Do not freeze\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eDocuments\u003c\/h5\u003e\n\u003ch4\u003eTechnical Note\u003c\/h4\u003e\n\u003cp\u003e🗎    \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Table_Binding_characteristics_of_immunoglobulin-binding_proteins.pdf?6231220717744860621\" title=\"Comparison of binding characteristics of immunoglobulin-binding proteins\" target=\"_blank\"\u003eComparison of Binding Characteristics of Immunoglobulin-Binding Proteins\u003c\/a\u003e\u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"10 mL","offer_id":40634725892139,"sku":"11-0297-010","price":1438.0,"currency_code":"USD","in_stock":true},{"title":"50 mL","offer_id":40634725924907,"sku":"11-0297-050","price":3748.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/11-0211ProteinAt.png?v=1678811997"},{"product_id":"proteindex-grav70-desalting-columns-with-g-25-medium-size-exclusive-resin","title":"GraviPur™15 Desalting Column with G-25 Medium Size Exclusion Resin for cleanup of 15 mL sample","description":"\u003cp\u003eGraviPur™ Desalting Columns are prepacked with PROTEINDEX™ SEC G-25 Cross-linked Dextran Resin, a molecular-weight–based selection resin designed for rapid and efficient removal of small molecules. With an effective cutoff of approximately Mr 5,000, these columns enable biomolecules larger than the exclusion limit—such as proteins, peptides, nucleic acids, and other macromolecules—to be cleanly separated from salts, detergents, and other low–molecular-weight contaminants.\u003c\/p\u003e\n\u003cp\u003eOptimized for convenience and reliability, GraviPur™ column body is made of bio-compatible polypropylene (PP), and the frits are hydrophilic, enabling fast flow through driven by gravity. A stopcock was provided with each column for better flow control during operation. The integrated Luer-lock outlet provides secure, direct connection to female Luer ports on standard vacuum manifolds. When used with the optional adapter (\u003ca rel=\"noopener\" title=\"Column adapter for additional column capacity and tubing connection\" href=\"https:\/\/marvelgent.com\/products\/column-connector-female-luer-to-30-60-70-column-polypropylene-pp\/\" target=\"_blank\"\u003eCat# 21-0336\u003c\/a\u003e, designed for 1\/8\" O.D. tubing, sold separately), the column can also be operated with peristaltic pumps or medium-pressure automated systems, offering enhanced versatility for different workflows.\u003c\/p\u003e\n\u003cp\u003eWith high sample-loading capacity and ready-to-use design, GraviPur™ Desalting Columns deliver a cost-effective, reliable, and easy-to-integrate solution for buffer exchange, desalting, and sample cleanup, ideal for routine laboratory applications or seamless incorporation into existing purification systems.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eProduct Highlights\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eEconomical, ready-to-use format for routine desalting and buffer exchange\u003c\/li\u003e\n\u003cli\u003eGentle, reliable separation of macromolecules from lower–molecular weight impurities\u003c\/li\u003e\n\u003cli\u003eSupports larger sample volumes for greater workflow flexibility\u003c\/li\u003e\n\u003cli\u003eSuitable for gravity-flow and vacuum-assisted operation\u003c\/li\u003e\n\u003cli\u003eCompatible with peristaltic pumps and medium-pressure automated systems when used with an optional tubing adapter\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Grav_desalting_columns_bf0e182b-bf31-49f1-98c8-165a0e1b42c5.png?v=1749519913\" alt=\"\" width=\"493\" height=\"415\" style=\"display: block; margin-left: auto; margin-right: auto;\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\" style=\"height: 321.2px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd width=\"200\" style=\"height: 19.6px; width: 36.6906%;\"\u003e\u003cstrong\u003eProduct package size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19.6px; width: 63.1295%;\"\u003e5 columns\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"height: 19.6px; width: 36.6906%;\"\u003e\u003cstrong\u003eMedium\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19.6px; width: 63.1295%;\"\u003eProteIndex™ SEC G-25 Medium Cross-Linked Dextran\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd width=\"200\" style=\"height: 19.6px; width: 36.6906%;\"\u003e\u003cstrong\u003eColumn bed volume\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19.6px; width: 63.1295%;\"\u003e50 mL \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"height: 19.6px; width: 36.6906%;\"\u003e\u003cstrong\u003eMaximum loading volume\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19.6px; width: 63.1295%;\"\u003e15 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"height: 19.6px; width: 36.6906%;\"\u003e\u003cstrong\u003eDesalination efficiency\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19.6px; width: 63.1295%;\"\u003e\u0026gt;90%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 16.9px;\"\u003e\n\u003ctd style=\"height: 16.9px; width: 36.6906%;\"\u003e\u003cstrong\u003eExclusion limit\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 16.9px; width: 63.1295%;\"\u003eMr 5000\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"height: 19.6px; width: 36.6906%;\"\u003e\u003cstrong\u003eGravity flow rate range \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19.6px; width: 63.1295%;\"\u003e10 – 20 mL\/min\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"height: 19.6px; width: 36.6906%;\"\u003e\u003cstrong\u003eMaximum pressure\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19.6px; width: 63.1295%;\"\u003e0.1 MPa (1 bar)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"height: 19.6px; width: 36.6906%;\"\u003e\u003cstrong\u003epH stability range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19.6px; width: 63.1295%;\"\u003e2 – 13 \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"height: 19.6px; width: 36.6906%;\"\u003e\u003cstrong\u003eStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19.6px; width: 63.1295%;\"\u003e2 – 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"height: 19.6px; width: 36.6906%;\"\u003e\u003cstrong\u003eStorage buffer\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19.6px; width: 63.1295%;\"\u003e0.1% ProClin 300 as preservative\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"height: 19.6px; width: 36.6906%;\"\u003e\u003cstrong\u003eColumn material\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19.6px; width: 63.1295%;\"\u003epolypropylene\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"height: 19.6px; width: 36.6906%;\"\u003e\u003cstrong\u003eFrit\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19.6px; width: 63.1295%;\"\u003ePolyethylene\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProduct is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e   \u003cimg style=\"float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Keep-refrigerated_95fa1867-1f0d-47e6-823a-8a0008076e21_small.png?12332456762384261517\" alt=\"Keep refrigerated\"\u003e   \u003cimg style=\"float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Do-not-freeze_2_small.png?12332456762384261517\" alt=\"Do not freeze\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"5 Columns","offer_id":42535987740715,"sku":"18-0323-15","price":495.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Emptycolumn-70ML_1_2s.png?v=1765404218"},{"product_id":"gravipur-6-desalting-column-with-g-25-medium-size-exclusion-resin-for-cleanup-of-6-ml-sample","title":"GraviPur™6 Desalting Column with G-25 Medium Size Exclusion Resin for cleanup of 6 mL sample","description":"\u003cp\u003eGraviPur™ Desalting Columns are prepacked with PROTEINDEX™ SEC G-25 Cross-linked Dextran Resin, a molecular-weight–based selection resin designed for rapid and efficient removal of small molecules. With an effective cutoff of approximately Mr 5,000, these columns enable biomolecules larger than the exclusion limit—such as proteins, peptides, nucleic acids, and other macromolecules—to be cleanly separated from salts, detergents, and other low–molecular-weight contaminants.\u003c\/p\u003e\n\u003cp\u003eOptimized for convenience and reliability, GraviPur™ column body is made of bio-compatible polypropylene (PP), and the frits are hydrophilic, enabling fast flow through driven by gravity. A stopcock was provided with each column for better flow control during operation. The integrated Luer-lock outlet provides secure, direct connection to female Luer ports on standard vacuum manifolds. When used with the optional adapter (Cat# \u003ca rel=\"noopener\" title=\"Column adapter for additional column capacity and tubing connection\" href=\"https:\/\/marvelgent.com\/products\/column-connector-female-luer-to-30-60-70-column-polypropylene-pp\" target=\"_blank\"\u003e21-0336\u003c\/a\u003e, designed for 1\/8\" O.D. tubing, sold separately), the column can also be operated with peristaltic pumps or medium-pressure automated systems, offering enhanced versatility for different workflows.\u003c\/p\u003e\n\u003cp\u003eWith their high sample-loading capacity and ready-to-use design, GraviPur™ Desalting Columns deliver a cost-effective, reliable, and easy-to-integrate solution for buffer exchange, desalting, and sample cleanup, ideal for routine laboratory applications or seamless incorporation into existing purification systems.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eProduct Highlights\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eEconomical, ready-to-use format for routine desalting and buffer exchange\u003c\/li\u003e\n\u003cli\u003eSupports larger sample volumes for greater workflow flexibility\u003c\/li\u003e\n\u003cli\u003eSuitable for gravity-flow and vacuum-assisted operation\u003c\/li\u003e\n\u003cli\u003eAdaptable to peristaltic pumps and medium-pressure automated systems with optional tubing adapter\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cimg height=\"415\" width=\"493\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Grav_desalting_columns_bf0e182b-bf31-49f1-98c8-165a0e1b42c5.png?v=1749519913\" style=\"display: block; margin-left: auto; margin-right: auto;\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\" style=\"height: 352.8px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd width=\"200\" style=\"height: 19.6px;\"\u003e\u003cstrong\u003eProduct package size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19.6px;\"\u003e10 columns\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd width=\"200\" style=\"height: 19.6px;\"\u003e\u003cstrong\u003eMedium\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19.6px;\"\u003eProteIndex™ SEC G-25 Medium Cross-Linked Dextran\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"height: 19.6px;\"\u003e\u003cstrong\u003eColumn bed volume\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19.6px;\"\u003e20 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"height: 19.6px;\"\u003e\u003cstrong\u003eMaximum loading volume\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19.6px;\"\u003e6 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"height: 19.6px;\"\u003e\u003cstrong\u003eDesalination efficiency\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19.6px;\"\u003e\u0026gt;90%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eExclusion limit\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMr 5000\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"height: 19.6px;\"\u003e\u003cstrong\u003eGravity flow rate range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19.6px;\"\u003e10 mL \/ min\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"height: 19.6px;\"\u003e\u003cstrong\u003eMaximum pressure\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19.6px;\"\u003e0.1 MPa (1 bar)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39.2px;\"\u003e\n\u003ctd valign=\"top\" style=\"height: 39.2px;\"\u003e\u003cstrong\u003epH stability range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 39.2px;\"\u003e2 – 13\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"height: 19.6px;\"\u003e\u003cstrong\u003eRStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19.6px;\"\u003eGravity flow, 100 - 300 cm\/hour\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd valign=\"top\" style=\"height: 19.6px;\"\u003e\u003cstrong\u003epH stability\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19.6px;\"\u003eLong term: pH 3 - 9; \u003cbr\u003eShort term: pH 2 - 10\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"height: 19.6px;\"\u003e\u003cstrong\u003eStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19.6px;\"\u003e2 – 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"height: 19.6px;\"\u003e\u003cstrong\u003eStorage buffer\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19.6px;\"\u003e0.1% ProClin 300 as preservative\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 58.8px;\"\u003e\n\u003ctd valign=\"top\" style=\"height: 58.8px;\"\u003e\u003cstrong\u003eColumn material\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 58.8px;\"\u003e\n\u003cp\u003ePolypropylene (body)\u003cbr\u003eLow density polyethylene (top snap cap and bottom tip cap)\u003cbr\u003ePolyethylene (frits)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProduct is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e   \u003cimg alt=\"Keep refrigerated\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Keep-refrigerated_95fa1867-1f0d-47e6-823a-8a0008076e21_small.png?12332456762384261517\" style=\"float: none;\"\u003e   \u003cimg alt=\"Do not freeze\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Do-not-freeze_2_small.png?12332456762384261517\" style=\"float: none;\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"10 Columns","offer_id":42536060289067,"sku":"18-0323-06","price":412.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Emptycolumn-70ML_1_2s.png?v=1765404218"},{"product_id":"magindex-ni-nta-magnetic-agarose-settled-resin","title":"MagINDEX™ Ni NTA Magnetic Agarose, Settled Resin","description":"\u003cp\u003e\u003cstrong\u003eMagINDEX™ Ni-NTA Magnetic Agarose Beads\u003c\/strong\u003e are high-capacity affinity beads engineered for the rapid purification and screening of 6xHis-tagged recombinant proteins. Built on Marvelgent’s proprietary agarose microsphere and surface chemistry platform, these beads combine the high-performance binding of Ni-NTA resin with the speed and simplicity of magnetic separation.\u003cbr\u003eMagnetic bead-based purification streamlines sample processing by eliminating column packing, centrifugation, and gravity-flow handling. Target proteins are captured directly from clarified lysates—whether from bacterial, insect, or mammalian sources—then rapidly separated, washed, and eluted. This format is ideal for:\u003cbr\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eRapid purification\u003c\/strong\u003e of His-tagged proteins (complete in under an hour).\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eParallel processing\u003c\/strong\u003e of multiple samples.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSmall sample volumes \u003c\/strong\u003ewhere loss must be minimized.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAutomation-compatible workflows\u003c\/strong\u003e where easy handling and reproducibility are essential.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eProduct Highlights\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eProprietary Agarose Coating\u003c\/strong\u003e \u003cbr\u003eOur unique coating technology minimizes magnetic particle shedding and leaching. This ensures maximum bead integrity without compromising the rapid magnetic responsiveness required for efficient capture and elution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eOptimized Surface Chemistry \u003c\/strong\u003e\u003cbr\u003eEngineered for efficient ligand coupling and robust Ni-NTA presentation, our surface chemistry provides enhanced capacity for His-tagged proteins.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eRapid Superparamagnetic Response \u003c\/strong\u003e\u003cbr\u003eThe beads respond to magnetic fields in \u0026lt; 2 seconds. This enables quick, clean separation, reduced hands-on time, and efficient processing of multiple samples.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSuperior Purity \u003c\/strong\u003e\u003cbr\u003eBy significantly reducing non-specific binding, the optimized MagINDEX™ surface ensures high-purity results in every eluate.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eVersatile Workflow Compatibility \u003c\/strong\u003e\u003cbr\u003eCompatible with standard tubes, multi-well plates, and automated magnetic separation platforms. This flexibility supports everything from individual research to high-throughput screening.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBroad Buffer Compatibility \u003c\/strong\u003e\u003cbr\u003eStable in a wide range of common purification buffers, including conditions for both native and denaturing His-tag purification.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eStringent QC \u0026amp; Lot-to-Lot Consistency\u003c\/strong\u003e \u003cbr\u003eEach lot is manufactured under highly controlled conditions and tested against rigorous quality standards to ensure reproducible performance across every experiment.\u003cbr\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ctable style=\"width: 100%; background-color: rgb(230, 230, 230);\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%;\"\u003e\u003ciframe src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/MagIndex_Magnetic_Agarose_Resins-3.mp4?14800512398908436554\"\u003e\u003c\/iframe\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 60%;\"\u003eThe superparamagnetic core provides anexceptionally fast response to magnetic fields, ensuring rapid separation with no residual magnetism once the field is removed. Our proprietary agarose-based encapsulation provides a robust shield that protects the magnetic core across a wide range of common protein purification reagents. This specialized design ensures high chemical stability while minimizing non-specific binding for superior sample purity.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eApplications\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eRapid purification \u003c\/strong\u003eof His-tagged recombinant proteins.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSmall-scale expression screening \u003c\/strong\u003eto identify high-yield clones.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh-throughput purification \u003c\/strong\u003efrom bacterial, insect, or mammalian lysates.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePull-down assays\u003c\/strong\u003e using His-tagged bait proteins to study molecular interactions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eNative or denaturing purification\u003c\/strong\u003e (refer to specifications for compatible agent ranges).\u003cbr\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cspan style=\"color: #666666;\"\u003e*Note: No significant decrease in yield after 8 repetitive uses of resin when recommended buffers are used. Refer to Product Details for compatible reagents.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Ni_NTA_magnetic_agarose2_255d0ddd-ed6f-43c0-8a90-139f30da1ffd.png?v=1778436974\" alt=\"\" width=\"237\" height=\"342\" style=\"display: block; margin-left: auto; margin-right: auto;\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFigure 1. Manual purification of an 80 kDa monomeric recombinant protein. \u003c\/strong\u003eAn 80 kDa monomeric His-tagged protein was purified from 50 mL of eukaryotic cell culture spent media using 4 mL of MagINDEX™ Ni-NTA Magnetic Agarose Beads. The sample was incubated for 30 minutes at room temperature with manual magnetic separation. The purified product was eluted with 250 mM imidazole and analyzed by SDS-PAGE under both reducing (+DTT) and non-reducing conditions. The results confirm high-yield recovery and the expected monomeric state of the target protein. Lane 1: Spent medium; Lane 2: Flow-through; Lane 3: Eluate (+DTT); Lane 4: Eluate (non-reduced).\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Ni_NTA_magnetic_agarose3.png?v=1778436977\" style=\"display: block; margin-left: auto; margin-right: auto;\" width=\"423\" height=\"288\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFigure 2. Automated high-throughput purification of His-tagged nanobodies. \u003c\/strong\u003eHis6-tagged nanobody clones (1–10; ~15 kDa) were expressed in HEK293 cells. Spent media (2 mL) were incubated with 800 µL of MagINDEX™ Ni-NTA Magnetic Agarose Beads for 30 minutes at room temperature using an automated magnetic separation platform. Samples were eluted with 250 mM imidazole and analyzed by SDS-PAGE. The gel demonstrates consistent recovery and high purity across all ten clones.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 25%;\"\u003e\u003cstrong\u003eLigand\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 75%;\"\u003eNickel\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 25%;\"\u003e\u003cstrong\u003eBinding capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 75%;\"\u003e40 mg 6xHis-tagged protein\/mL medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 25%;\"\u003e\u003cstrong\u003eMatrix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 75%;\"\u003eSuperparamagnetic agarose beads\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 25%;\"\u003e\u003cstrong\u003eParticle size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 75%;\"\u003e30 μm - 100 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 25%;\"\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 75%;\"\u003e20% suspension\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 25%;\" valign=\"top\"\u003e\u003cstrong\u003eChemical compatibility\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 75%;\"\u003e\n\u003cp\u003e\u003cspan style=\"text-decoration: underline;\"\u003eReducing agents\u003c\/span\u003e:\u003cbr\u003e5 mM DTE, 0.5-1 mM DTT, 20 mM β-mercaptoethanol, 5 mM TCEP, 10 mM reduced glutathione (GSH)\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"text-decoration: underline;\"\u003eDenaturing agents\u003c\/span\u003e:\u003cbr\u003e8 M urea, 6 M Gua-HCl (Tested for one week at 40°C)\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"text-decoration: underline;\"\u003eDetergents\u003c\/span\u003e:\u003cbr\u003e2% Triton™ X-100 (nonionic), 2% Tween™ 20 (nonionic), 2% NP-40 (nonionic), 2% cholate (anionic), 1% CHAPS (zwitterionic)\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"text-decoration: underline;\"\u003eOther additives\u003c\/span\u003e:\u003cbr\u003e500 mM imidazole, 20% ethanol, 50% glycerol, 100 mM Na\u003csub\u003e2\u003c\/sub\u003eSO4, 1.5 M NaCl, 1 mM EDTA, 60 mM citrate\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"text-decoration: underline;\"\u003eCommonly used buffer:\u003c\/span\u003e\u003cbr\u003e50 mM sodium phosphate (pH 7.4), 100 mM Tris-HCl (pH 7.4), 100 mM Tris-acetate (pH 7.4), 100 mM HEPES (pH 7.4), 100 mM MOPS (pH 7.4), 100 mM sodium acetate (pH 4)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 25%;\"\u003e\u003cstrong\u003eStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 75%;\"\u003e2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 25%;\"\u003e\u003cstrong\u003eStorage buffer\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 75%;\"\u003e1x PBS containing 20% ethanol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProduct is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e   \u003cimg style=\"float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Keep-refrigerated_95fa1867-1f0d-47e6-823a-8a0008076e21_small.png?12332456762384261517\" alt=\"Keep refrigerated\" width=\"52\" height=\"50\"\u003e   \u003cimg height=\"30\" width=\"52\" style=\"float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Do-not-freeze_2_small.png?12332456762384261517\" alt=\"Do not freeze\"\u003e\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e \u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"2 mL","offer_id":42661596266539,"sku":"11-0226-002","price":165.0,"currency_code":"USD","in_stock":true},{"title":"10 mL","offer_id":42661497176107,"sku":"11-0226-010","price":750.0,"currency_code":"USD","in_stock":true},{"title":"100 mL","offer_id":42661497241643,"sku":"11-0226-100","price":5250.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Ni-NTA_magnetic_beads-4.png?v=1774387549"},{"product_id":"gravipur-50-desalting-column-with-g-25-medium-size-exclusion-resin-for-cleanup-of-50-ml-sample","title":"GraviPur™50 Desalting Column with G-25 Medium Size Exclusion Resin for cleanup of 50 mL sample","description":"\u003cp\u003eGraviPur™ Desalting Columns are prepacked with PROTEINDEX™ SEC G-25 Cross-linked Dextran Resin, a molecular-weight–based selection resin designed for rapid and efficient removal of small molecules. With an effective cutoff of approximately Mr 5,000, these columns enable biomolecules larger than the exclusion limit—such as proteins, peptides, nucleic acids, and other macromolecules—to be cleanly separated from salts, detergents, and other low–molecular-weight contaminants.\u003c\/p\u003e\n\u003cp\u003eThe column body is made of bio-compatible polypropylene (PP), and the frits are hydrophilic, enabling fast flow through driven by gravity. The integrated Luer-lock outlet provides secure, direct connection to female Luer ports on standard vacuum manifolds. A stopcock was provided with each column for better flow control during operation.\u003c\/p\u003e\n\u003cp\u003eWith high sample-loading capacity and ready-to-use design, GraviPur™ Desalting Columns deliver a cost-effective, reliable, and easy-to-integrate solution for buffer exchange, desalting, and sample cleanup, ideal for routine laboratory applications or seamless incorporation into existing purification systems.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eProduct Highlights\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eEconomical, ready-to-use format for routine desalting and buffer exchange\u003c\/li\u003e\n\u003cli\u003eGentle, reliable separation of macromolecules from lower–molecular weight impurities\u003c\/li\u003e\n\u003cli\u003eSupports larger sample volumes for greater workflow flexibility\u003c\/li\u003e\n\u003cli\u003eSuitable for gravity-flow and vacuum-assisted operation\u003cbr\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" height=\"415\" width=\"493\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Grav_desalting_columns_bf0e182b-bf31-49f1-98c8-165a0e1b42c5.png?v=1749519913\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable style=\"height: 321.2px;\" width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"height: 19.6px; width: 36.6906%;\" width=\"200\"\u003e\u003cstrong\u003eProduct package size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19.6px; width: 63.1295%;\"\u003e2 columns\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"height: 19.6px; width: 36.6906%;\"\u003e\u003cstrong\u003eMedium\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19.6px; width: 63.1295%;\"\u003eProteIndex™ SEC G-25 Medium Cross-Linked Dextran\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"height: 19.6px; width: 36.6906%;\" width=\"200\"\u003e\u003cstrong\u003eColumn bed volume\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19.6px; width: 63.1295%;\"\u003e166 mL \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"height: 19.6px; width: 36.6906%;\"\u003e\u003cstrong\u003eMaximum loading volume\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19.6px; width: 63.1295%;\"\u003e50 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"height: 19.6px; width: 36.6906%;\"\u003e\u003cstrong\u003eDesalination efficiency\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19.6px; width: 63.1295%;\"\u003e\u0026gt;90%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 16.9px;\"\u003e\n\u003ctd style=\"height: 16.9px; width: 36.6906%;\"\u003e\u003cstrong\u003eExclusion limit\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 16.9px; width: 63.1295%;\"\u003eMr 5000\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"height: 19.6px; width: 36.6906%;\"\u003e\u003cstrong\u003eGravity flow rate range \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19.6px; width: 63.1295%;\"\u003e10 – 20 mL\/min\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"height: 19.6px; width: 36.6906%;\"\u003e\u003cstrong\u003eMaximum pressure\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19.6px; width: 63.1295%;\"\u003e0.1 MPa (1 bar)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"height: 19.6px; width: 36.6906%;\"\u003e\u003cstrong\u003epH stability range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19.6px; width: 63.1295%;\"\u003e2 – 13 \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"height: 19.6px; width: 36.6906%;\"\u003e\u003cstrong\u003eStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19.6px; width: 63.1295%;\"\u003e2 – 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"height: 19.6px; width: 36.6906%;\"\u003e\u003cstrong\u003eStorage buffer\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19.6px; width: 63.1295%;\"\u003e0.1% ProClin 300 as preservative\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"height: 19.6px; width: 36.6906%;\"\u003e\u003cstrong\u003eColumn material\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19.6px; width: 63.1295%;\"\u003epolypropylene\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd style=\"height: 19.6px; width: 36.6906%;\"\u003e\u003cstrong\u003eFrit\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19.6px; width: 63.1295%;\"\u003ePolyethylene\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProduct is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e   \u003cimg alt=\"Keep refrigerated\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Keep-refrigerated_95fa1867-1f0d-47e6-823a-8a0008076e21_small.png?12332456762384261517\" style=\"float: none;\"\u003e   \u003cimg alt=\"Do not freeze\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Do-not-freeze_2_small.png?12332456762384261517\" style=\"float: none;\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"2 Columns","offer_id":42700656246827,"sku":"18-0323-50","price":395.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/Marvelgent_Biosciences_products_3_2ec02dde-fe72-481b-bf07-eb84610c5b01.png?v=1677085522"}],"url":"https:\/\/marvelgent.com\/collections\/featured-products.oembed?page=2","provider":"Marvelgent Biosciences","version":"1.0","type":"link"}