{"title":"Chromatography Resins","description":"\u003cp\u003eMarvelgent provides agarose-based and polymer-based chromatography resins designed for efficient capture, purification, and polishing of proteins and biomolecules.\u003cbr\u003eBuilt on reliable base matrices, outperforming via optimized ligand chemistries, and assured by stringent quality control, Marvelgent chromatography resins support a broad range of applications from early-stage research and method development to pilot-scale and full scale-up production.\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ch2\u003eChromatography Resin Technology\u003c\/h2\u003e\n\u003cp\u003eChromatography resins are porous separation media used to purify proteins, antibodies, enzymes, nucleic acids, viral particles, and other biomolecules. By combining a solid base matrix with specific surface chemistries or ligands, chromatography resins separate target molecules based on affinity, charge, hydrophobicity, size, or mixed interaction mechanisms.\u003cbr\u003eModern resin technologies are central to every step in the purification workflows. A typical process may include:\u003cbr\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eCapture \u003c\/strong\u003eto enrich the target molecule from crude sample\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eIntermediate purification\u003c\/strong\u003e to remove major impurities\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePolishing \u003c\/strong\u003eto improve purity, remove aggregates, and reduce contaminants\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBuffer exchange, desalting, or sample preparation\u003c\/strong\u003e for downstream analysis or formulation\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChoosing the right resin chemistry and format is essential for achieving high recovery, purity, reproducibility, and process efficiency. \u003cspan style=\"text-decoration: underline; color: rgb(29, 39, 149);\"\u003eExplore our resin base matrix families and learn more on our resin technologies here.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ch2\u003eMarvelgent Resin Platform\u003c\/h2\u003e\n\u003cp\u003eMarvelgent offers a comprehensive chromatography resin platform centered on reliable agarose resin technology and complemented by polymer-based and specialty resin solutions. Our portfolio includes affinity, ion exchange, hydrophobic interaction, size exclusion, multimodal, and activated resins to support diverse purification needs.\u003cbr\u003eOur platform is designed to help researchers and process-development teams select practical, high-performance purification solutions for both routine and challenging biomolecule purification.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003ePlatform Advantages\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eReliable resin technology:\u003c\/strong\u003e Marvelgent resins are designed to provide dependable binding performance, favorable flow properties, and broad compatibility with common protein purification workflows.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eInnovative solutions for challenging purification: \u003c\/strong\u003eOur resin development focuses on difficult-to-purify proteins, complex samples, and demanding process conditions. By combining optimized base matrices, ligand chemistries, and surface properties, Marvelgent provides specialized solutions—such as chemical tolerant \u003cstrong\u003eNi-Penta\u003c\/strong\u003e resin for His-tagged protein purification and \u003cstrong\u003eGentle-Elution Protein A \u003c\/strong\u003eresin for pH-sensitive antibody purification—for applications where standard purification methods may not be sufficient.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBroad chromatography modes from research to scale-up: \u003c\/strong\u003eThe Marvelgent portfolio covers major chromatography modes. This breadth supports purification workflows from early research and screening to process development and scale-up purification.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eStrong value \/ performance balance: \u003c\/strong\u003eMarvelgent resins are developed to deliver reliable purification performance with practical cost efficiency, helping laboratories and process teams balance quality, productivity, and budget requirements.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eLot consistency: \u003c\/strong\u003eStringent quality control helps ensure consistent resin properties and reproducible performance from lot to lot, supporting reliable method development and process transfer.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eRegulatory and application support: \u003c\/strong\u003eMarvelgent provides product documentation, technical information, and regulatory support documents to assist customers in resin selection, process development, validation, scale-up planning and regulatory submissions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eResponsive technical support: \u003c\/strong\u003eOur technical team works closely with customers to recommend suitable resin types, troubleshoot purification challenges, and support method optimization.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlexible formats: \u003c\/strong\u003eMarvelgent resins are available in flexible formats, including loose resins, pre-packed cartridges, and related magnetic bead formats for screening, method development, and purification workflow optimization.\u003cbr\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003eResin Selection by Purification Workflow\u003c\/h4\u003e\n\u003ctable align=\"center\" width=\"100%\" style=\"height: 274.4px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 39.2px;\"\u003e\n\u003ctd width=\"30%\" style=\"background: rgb(238, 238, 238); text-align: left; height: 39.2px;\"\u003e\u003cstrong\u003eWorkflow Step\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"40%\" style=\"background: rgb(238, 238, 238); text-align: left; height: 39.2px;\"\u003e\u003cstrong\u003eMain Goal\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"30%\" style=\"background: rgb(238, 238, 238); text-align: left; height: 39.2px;\"\u003e\u003cstrong\u003eRecommended Resin Modes\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 58.8px;\"\u003e\n\u003ctd valign=\"top\" style=\"height: 58.8px;\"\u003e\u003cstrong\u003eCapture\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 58.8px;\"\u003eBind and enrich the target molecule from crude or partially clarified samples\u003c\/td\u003e\n\u003ctd valign=\"top\" style=\"text-align: left; height: 58.8px;\"\u003eAffinity, Ion Exchange (IEX)\u003csup\u003e\u003c\/sup\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 58.8px;\"\u003e\n\u003ctd valign=\"top\" style=\"height: 58.8px;\"\u003e\u003cstrong\u003eIntermediate Purification\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 58.8px;\"\u003eRemove major impurities while maintaining target recovery\u003c\/td\u003e\n\u003ctd style=\"text-align: left; height: 58.8px;\"\u003eIEX, Hydrophobic Interaction (HIC), Multimodal \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 58.8px;\"\u003e\n\u003ctd valign=\"top\" style=\"height: 58.8px;\"\u003e\u003cstrong\u003ePolishing\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 58.8px;\"\u003eRemove trace impurities, aggregates, or closely related contaminants\u003c\/td\u003e\n\u003ctd style=\"text-align: left; height: 58.8px;\"\u003eIEX, Size Exclusion (SEC), multimodal \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 58.8px;\"\u003e\n\u003ctd valign=\"top\" style=\"height: 58.8px;\"\u003e\u003cstrong\u003eBuffer Exchange \/ Desalting\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 58.8px;\"\u003eDesalting or sample preparation for downstream analysis\u003c\/td\u003e\n\u003ctd valign=\"top\" style=\"text-align: left; height: 58.8px;\"\u003eSEC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003eResin Selection Guide by Functionality\u003c\/h4\u003e\n\u003ctable align=\"center\" width=\"100%\" style=\"height: 274.4px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 39.2px;\"\u003e\n\u003ctd width=\"30%\" style=\"background: rgb(238, 238, 238); text-align: left; height: 39.2px;\"\u003e\u003cstrong\u003eResin Mode\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"40%\" style=\"background: rgb(238, 238, 238); text-align: left; height: 39.2px;\"\u003e\u003cstrong\u003eSeparation Principle\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"30%\" style=\"background: rgb(238, 238, 238); text-align: left; height: 39.2px;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 58.8px;\"\u003e\n\u003ctd valign=\"top\" style=\"height: 58.8px;\"\u003e\u003cstrong\u003eAffinity Chromatography\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd valign=\"top\" style=\"height: 58.8px;\"\u003eSpecific interaction between target molecule and ligand\u003c\/td\u003e\n\u003ctd valign=\"top\" style=\"text-align: left; height: 58.8px;\"\u003eHigh-selectivity capture of tagged proteins, antibodies, fusion proteins, and specific biomolecules\u003csup\u003e\u003c\/sup\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eIon Exchange Chromatography (IEX)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003eSeparation based on molecular charge\u003c\/td\u003e\n\u003ctd style=\"text-align: left;\"\u003eProtein capture, impurity removal, charge-variant separation, intermediate purification, polishing\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eHydrophobic Interaction Chromatography (HIC)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003eSeparation based on hydrophobicity under salt-controlled conditions\u003c\/td\u003e\n\u003ctd style=\"text-align: left;\"\u003eProtein polishing, aggregate removal, orthogonal purification after affinity or IEX steps\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eSize Exclusion Chromatography (SEC)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003eSeparation based on molecular size\u003c\/td\u003e\n\u003ctd style=\"text-align: left;\"\u003eDesalting, buffer exchange, aggregate analysis, polishing, sample preparation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eMultimodal Chromatography \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eCombined interaction mechanisms such as charge, hydrophobicity, hydrogen bonding, or metal affinity\u003c\/td\u003e\n\u003ctd style=\"text-align: left;\"\u003eChallenging separations, impurity clearance, polishing, process simplification\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eActivated Resins\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003eReactive groups enable custom ligand coupling\u003c\/td\u003e\n\u003ctd style=\"text-align: left;\"\u003eCustom affinity resin preparation, immobilization, assay development, specialty purification\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ch2\u003eExplore Marvelgent Chromatography Resin Families\u003c\/h2\u003e\n\u003ch4\u003e\u003cspan style=\"text-decoration: underline;\"\u003eAffinity Chromatography Resins\u003c\/span\u003e\u003c\/h4\u003e\n\u003cp\u003eMarvelgent affinity resins are designed for high-selectivity purification of tagged proteins, antibodies, fusion proteins, and other target molecules. Our affinity portfolio includes IMAC resins for His-tagged proteins, Protein A\/G\/L resins for antibody purification, and ligand-specific resins for common fusion tags and biomolecular targets.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTypical applications include:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eHis-tagged recombinant protein purification\u003c\/li\u003e\n\u003cli\u003eAntibody and Fc-fusion protein purification\u003c\/li\u003e\n\u003cli\u003eGST, Strep-tag, FLAG-tag, HA-tag, c-Myc-tag, and fluorescent protein purification\u003c\/li\u003e\n\u003cli\u003eCustom affinity purification using activated resin chemistries\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ctable align=\"center\" width=\"100%\" style=\"height: 274.4px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 39.2px;\"\u003e\n\u003ctd width=\"29%\" style=\"background: rgb(238, 238, 238); text-align: left; height: 39.2px;\"\u003eHis–tag fusion protein purification\u003c\/td\u003e\n\u003ctd width=\"33%\" style=\"background: rgb(238, 238, 238); text-align: left; height: 39.2px;\"\u003eAntibody purification\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd width=\"38%\" style=\"background: rgb(238, 238, 238); text-align: left; height: 39.2px;\"\u003eOther fusion Protein and molecules purification\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 58.8px;\"\u003e\n\u003ctd valign=\"top\" style=\"height: 58.8px;\"\u003e\n\u003cul\u003e\n\u003cli\u003eNi-NTA\u003c\/li\u003e\n\u003cli style=\"font-weight: bold;\"\u003e\u003cstrong\u003eNi-Penta\u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003eCo-NTA\u003c\/li\u003e\n\u003cli\u003eNi-IDA\u003c\/li\u003e\n\u003cli\u003eCu-IDA*\u003c\/li\u003e\n\u003cli\u003eZn-IDA*\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/td\u003e\n\u003ctd style=\"height: 58.8px;\" valign=\"top\"\u003e\n\u003cul\u003e\n\u003cli\u003eProtein A\u003c\/li\u003e\n\u003cli\u003eProtein A alkaline stable\u003c\/li\u003e\n\u003cli\u003eProtein A super alkaline stable*\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGentle-Elution Protein A\u003c\/strong\u003e *\u003c\/li\u003e\n\u003cli\u003eProtein G\u003c\/li\u003e\n\u003cli\u003eProtein A\/G\u003c\/li\u003e\n\u003cli\u003eProtein L\u003c\/li\u003e\n\u003cli\u003eSulfhydryl-reactive resin*\u003c\/li\u003e\n\u003cli\u003eNHS-Activated resin*\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" style=\"text-align: left; height: 58.8px;\"\u003e\n\u003cul\u003e\n\u003cli\u003eGlutathione\u003c\/li\u003e\n\u003cli\u003eDextrin\u003c\/li\u003e\n\u003cli\u003eAnti-DYKDDDDK\u003c\/li\u003e\n\u003cli\u003eStreptavidin\u003c\/li\u003e\n\u003cli\u003eStrep-tag and Twin Strep-tag*\u003c\/li\u003e\n\u003cli\u003eAnti-HA*\u003c\/li\u003e\n\u003cli\u003eAnti-c-Myc*\u003c\/li\u003e\n\u003cli\u003eAnti-GFP*\u003c\/li\u003e\n\u003cli\u003eAnti-RFP*\u003c\/li\u003e\n\u003cli\u003eAnti-YFP*\u003c\/li\u003e\n\u003cli\u003eAnti-HSA (Human Serum Albumin)*\u003c\/li\u003e\n\u003cli\u003eAnti- carbohydrates*\u003c\/li\u003e\n\u003cli\u003eAnti-endotoxin*\u003c\/li\u003e\n\u003cli\u003eAnti-LCA*\u003c\/li\u003e\n\u003cli\u003eAnti- Heparin*\u003c\/li\u003e\n\u003cli\u003eAnti-AAV virus*\u003csup\u003e\u003c\/sup\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e* Please request\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"text-decoration: underline;\"\u003eIon Exchange Chromatography Resins\u003c\/span\u003e\u003c\/h4\u003e\n\u003cp\u003eMarvelgent ion exchange resins support protein purification based on charge interactions and are widely used in capture, intermediate purification, and polishing steps. Our anion and cation exchange resin families provide flexible options for method development and scale-up purification.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTypical applications include:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eProtein and enzyme purification\u003c\/li\u003e\n\u003cli\u003eRemoval of host-cell proteins and charged impurities\u003c\/li\u003e\n\u003cli\u003eCharge-variant separation\u003c\/li\u003e\n\u003cli\u003eIntermediate purification and polishing\u003c\/li\u003e\n\u003cli\u003eProcess development for recombinant proteins and biologics\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ctable align=\"center\" width=\"100%\" style=\"height: 274.4px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 39.2px;\"\u003e\n\u003ctd width=\"29%\" style=\"background: rgb(238, 238, 238); text-align: left; height: 39.2px;\"\u003eAnion\u003c\/td\u003e\n\u003ctd width=\"33%\" style=\"background: rgb(238, 238, 238); text-align: left; height: 39.2px;\"\u003eCation\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd width=\"38%\" style=\"background: rgb(238, 238, 238); text-align: left; height: 39.2px;\"\u003ePolymer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cul\u003e\n\u003cli\u003eQ Beads 6FF\u003c\/li\u003e\n\u003cli\u003eMarvarose Q\u003c\/li\u003e\n\u003cli\u003eMarvarose Q 40*\u003c\/li\u003e\n\u003cli\u003eQ Big Beads*\u003c\/li\u003e\n\u003cli\u003eDEAE Beads 6FF\u003c\/li\u003e\n\u003cli\u003eDEAE Dextran G25*\u003cbr\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cul\u003e\n\u003cli\u003eSP Beads 6FF\u003c\/li\u003e\n\u003cli\u003eMarvarose SP\u003c\/li\u003e\n\u003cli\u003eMarvarose SP40*\u003c\/li\u003e\n\u003cli\u003eSP Big Beads*\u003c\/li\u003e\n\u003cli\u003eCM Beads 6FF\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cul\u003e\n\u003cli\u003ePolyAcrylate SP 70S*\u003c\/li\u003e\n\u003cli\u003ePolyAcrylate SP 70M*\u003c\/li\u003e\n\u003cli\u003ePolyAcrylate SP 70L*\u003c\/li\u003e\n\u003cli\u003ePolyAcrylate Q 70S*\u003c\/li\u003e\n\u003cli\u003ePolyAcrylate Q 70M*\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003ePolyAcrylate Q 70L*\u003c\/li\u003e\n\u003cli\u003ePolyAcrylate CM 70S*\u003c\/li\u003e\n\u003cli\u003ePolyAcrylate CM 70M*\u003c\/li\u003e\n\u003cli\u003ePolyAcrylate CM 70L*\u003c\/li\u003e\n\u003cli\u003ePolyAcrylate 15Q*\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e* Please request\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"text-decoration: underline;\"\u003eHydrophobic Interaction Chromatography Resins\u003c\/span\u003e\u003c\/h4\u003e\n\u003cp\u003eMarvelgent HIC resins separate proteins based on hydrophobic interaction and provide an orthogonal purification mechanism to affinity and ion exchange chromatography. They are especially useful for improving purity, removing aggregates, and optimizing downstream purification workflows.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTypical applications include:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eProtein polishing\u003c\/li\u003e\n\u003cli\u003eAggregate and impurity reduction\u003c\/li\u003e\n\u003cli\u003eAntibody and recombinant protein purification\u003c\/li\u003e\n\u003cli\u003eOrthogonal purification after affinity or IEX capture\u003cbr\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ctable align=\"center\" width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" style=\"width: 33%;\"\u003e\n\u003cul\u003e\n\u003cli\u003eButyl Beads 4FF\u003c\/li\u003e\n\u003cli\u003eButyl Beads HiFlow*\u003c\/li\u003e\n\u003cli\u003eMarvorose Butyl 40*\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003eButyl Marvorose Beads*\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" style=\"width: 33%;\"\u003e\n\u003cul\u003e\n\u003cli\u003eOctyl Beads 4FF\u003c\/li\u003e\n\u003cli\u003eOctyl Beads HiFlow*\u003c\/li\u003e\n\u003cli\u003eMarvarose Octyl 40*\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003eOctyl Marvarose Beads*\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" style=\"width: 34%;\"\u003e\n\u003cul\u003e\n\u003cli\u003ePhenyl Beads 6FF (Low Sub)\u003c\/li\u003e\n\u003cli\u003ePhenyl Beads HF (Low Sub)*\u003c\/li\u003e\n\u003cli\u003ePhenyl Beads 6FF (High Sub)\u003c\/li\u003e\n\u003cli\u003ePhenyl Beads HF( High Sub)*\u003cbr\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e* Please request\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"text-decoration: underline;\"\u003eSize Exclusion Chromatography Resins\u003c\/span\u003e\u003c\/h4\u003e\n\u003cp\u003eMarvelgent size exclusion chromatography resins are designed for separation based on molecular size. They support desalting, buffer exchange, group separation, and polishing applications for proteins and other biomolecules.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTypical applications include:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eDesalting and buffer exchange\u003c\/li\u003e\n\u003cli\u003eProtein sample preparation\u003c\/li\u003e\n\u003cli\u003eRemoval of small molecules\u003c\/li\u003e\n\u003cli\u003eAggregate analysis and polishing\u003c\/li\u003e\n\u003cli\u003ePurification of biomolecules by size range\u003cbr\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ctable align=\"center\" width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"33%\"\u003e\n\u003cul\u003e\n\u003cli\u003eG-10*\u003c\/li\u003e\n\u003cli\u003eG-15*\u003c\/li\u003e\n\u003cli\u003eG-25 Coarse\u003c\/li\u003e\n\u003cli\u003eG-25 Medium\u003c\/li\u003e\n\u003cli\u003eG-25 Fine\u003c\/li\u003e\n\u003cli\u003eG-50*\u003c\/li\u003e\n\u003cli\u003eG-75*\u003c\/li\u003e\n\u003cli\u003eG-100*\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"33%\"\u003e\n\u003cul\u003e\n\u003cli\u003e4B*\u003c\/li\u003e\n\u003cli\u003e6B*\u003c\/li\u003e\n\u003cli\u003eCL-4B*\u003c\/li\u003e\n\u003cli\u003eCL-6B*\u003c\/li\u003e\n\u003cli\u003e4 Fast Flow*\u003c\/li\u003e\n\u003cli\u003e6 Fast Flow*\u003c\/li\u003e\n\u003cli\u003eLH-20*\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e* Please request\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"text-decoration: underline;\"\u003eMultimodal Chromatography Resins\u003c\/span\u003e\u003c\/h4\u003e\n\u003cp\u003eMarvelgent multimodal resins provide combined interaction mechanisms to address difficult separations that cannot be efficiently resolved by single-mode chromatography. These resins can help improve impurity clearance, simplify purification workflows, and support challenging protein purification applications.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTypical applications include:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eDifficult protein purification\u003c\/li\u003e\n\u003cli\u003eImpurity and aggregate removal\u003c\/li\u003e\n\u003cli\u003eProcess intensification\u003c\/li\u003e\n\u003cli\u003ePolishing of complex biomolecules\u003c\/li\u003e\n\u003cli\u003eOrthogonal purification method development\u003cbr\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ctable style=\"width: 100%;\" align=\"center\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%;\" valign=\"top\"\u003e\n\u003cul\u003e\n\u003cli\u003eMarvorose 700*\u003c\/li\u003e\n\u003cli\u003eMarvorose 400*\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003eMarvorose MMA*\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003eMarvorose MMA 40*\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003eMarvorose MMC*\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003eMarvorose MMC 40*\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 40%;\" valign=\"top\"\u003e\n\u003cul\u003e\n\u003cli\u003eHydroxyapatite 60（CHT I 60）*\u003c\/li\u003e\n\u003cli\u003eHydroxyapatite 60（CHTII 60）*\u003c\/li\u003e\n\u003cli\u003eHydroxyapatite 40（CHT I 40）*\u003c\/li\u003e\n\u003cli\u003eHydroxyapatite 40（CHTII 40）*\u003c\/li\u003e\n\u003cli\u003eHydroxyapatite 20（CHT I 20）*\u003c\/li\u003e\n\u003cli\u003eHydroxyapatite 20（CHTII 20）*\u003cbr\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e* Please request\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"text-decoration: underline;\"\u003eActivated Resins for Custom Coupling\u003c\/span\u003e\u003c\/h4\u003e\n\u003cp\u003eMarvelgent activated resins provide reactive chemistries for immobilizing proteins, peptides, antibodies, antigens, enzymes, and other ligands. These resins are suitable for custom affinity resin development, assay preparation, and specialty purification workflows.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTypical applications include:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eCustom ligand immobilization\u003c\/li\u003e\n\u003cli\u003eAntibody or antigen coupling\u003c\/li\u003e\n\u003cli\u003eEnzyme immobilization\u003c\/li\u003e\n\u003cli\u003eCustom affinity purification\u003c\/li\u003e\n\u003cli\u003eResearch assay and method development\u003cbr\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ctable align=\"center\" width=\"100%\" style=\"width: 100.036%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" style=\"width: 40%;\"\u003e\n\u003cul\u003e\n\u003cli\u003eSulfhydryl-Reactive  4B\/4FF*\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003eNHS-Activated 4FF*\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003eNHS-LongArm Activated 4FF*\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003eEpoxy-Activated 4FF\/6B*\u003cbr\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" style=\"width: 60%;\"\u003e\n\u003cul\u003e\n\u003cli\u003eCarboxyl-Activated Magnetic Polymer Particles*\u003c\/li\u003e\n\u003cli\u003eEthyl-Activated 4FF*\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003eAldehyde-Activated 4FF*\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003eAmino-Activated 4FF*\u003cbr\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e* Please request\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ch2\u003eNeed Help Choosing a Resin?\u003c\/h2\u003e\n\u003cp\u003eChoosing the right resin depends on the target molecule, sample type, purification goal, buffer conditions, required purity, and scale. Marvelgent provides responsive technical support to help customers select appropriate resin chemistries, compare purification strategies, and optimize workflows.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eContact Marvelgent for:\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eResin selection support\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003eBulk resin inquiries\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003ePre-packed cartridge options\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003eApplication guidance\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003eCustom resin development\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eMarvelgent chromatography resins are built to support reliable purification—from research development to scale-up workflows—with strong performance, consistent quality, and practical value.\u003c\/strong\u003e\u003c\/p\u003e","products":[{"product_id":"proteindex-glutathione-agarose-4-fast-flow","title":"PROTEINDEX™ Glutathione Agarose 4 Fast Flow","description":"\u003cp\u003e\u003cstrong\u003ePROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e\u003c\/strong\u003e™ Glutathione Agarose 4FF is an affinity chromatography medium developed for easy, one-step purification of recombinant glutathione S-transferase (GST) fusion proteins and other glutathione binding proteins expressed in \u003cem\u003eE. coli\u003c\/em\u003e, insect and mammalian cells. The rigid, highly cross-linked support is suitable for purification scale-up applications.\u003c\/p\u003e\n\u003cp\u003eOur proprietary ProteIndex™ coupling technology ensures stable immobilization of active ligand and minimal ligand leakage in a wide range of purification buffer conditions. The resins can be reused multiple times to purify the same target protein.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eProduct Highlights\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eHigh selectivity for high purity\u003c\/li\u003e\n\u003cli\u003e\u0026gt; 10 mg GST\/mL settled resins\u003c\/li\u003e\n\u003cli\u003eStable coupling of ligand, low leakage; can be re-used to purify the same protein\u003c\/li\u003e\n\u003cli\u003eVersatile, ready-to-use with convenient formats\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"10%\"\u003e\u003c\/td\u003e\n\u003ctd width=\"40%\"\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/GST_Beads_4FF2017050703_2.png?v=1508427878\"\u003e\u003c\/td\u003e\n\u003ctd valign=\"bottom\"\u003e\n\u003cp\u003e\u003cstrong\u003ePurification performance of Glutathione 4FF resins.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e50 mL of E.coli lysate containing a GST-tagged fusion protein was loaded on 0.5 mL packed Glutathione 4FF resins at 0.5 mL\/mL. The column was then washed with 10 mL of PBS. The target GST-tagged protein was eluted with 7.5 mL of elution buffer (50 mM Tris-HCl, 10 mM GSH, pH 8.0). Fractions of lysate, flow-through, and eluate were analysed by SDS-PAGE. Lanes: (1) Raw cell lysate; (2) Flow-through; (3) Eluate.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"200\"\u003e\u003cstrong\u003eLigand\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eGlutathione coupled with a 12-atom linker\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eLigand concentration \u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e120 - 320 μmol Glutathione\/mL medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBinding capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;10 mg GST (40 kDa)\/mL medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMatrix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e4% highly cross-linked agarose supplied as 50% slurry\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eParticle size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e45 μm - 165 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaximum pressure\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.3 MPa (3 bar, 43 psi)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eRecommended flow rate\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003e300 - 500 cm\/hour\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003epH stability\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003e3-12\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage buffer\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e20% ethanol as preservative\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProduct is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e   \u003cimg style=\"float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Keep-refrigerated_95fa1867-1f0d-47e6-823a-8a0008076e21_small.png?12332456762384261517\" alt=\"Keep refrigerated\"\u003e   \u003cimg style=\"float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Do-not-freeze_2_small.png?12332456762384261517\" alt=\"Do not freeze\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"10 mL","offer_id":14463117892,"sku":"11-0199-010","price":248.0,"currency_code":"USD","in_stock":true},{"title":"50 mL","offer_id":14463117956,"sku":"11-0199-050","price":935.0,"currency_code":"USD","in_stock":true},{"title":"100 mL","offer_id":14463118084,"sku":"11-0199-100","price":1716.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/11-0198_Glutathione_Agarose.png?v=1491948363"},{"product_id":"proteindex-rprotein-a-agarose-4ff","title":"PROTEINDEX™ rProtein A Agarose 4 Fast Flow","description":"\u003cp\u003e\u003cstrong\u003ePROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e\u003c\/strong\u003e™ rProtein A Agarose 4 Fast Flow is an affinity chromatography medium designed for easy, one-step purification of immunoglobulins, notably IgG isotypes, as well as Fc region-containing fusion proteins.\u003c\/p\u003e\n\u003cp\u003eProtein A, a bacterial cell wall protein isolated from \u003cem\u003eStaphylococcus aureus\u003c\/em\u003e, binds to the Fc region of most immunoglobulins through interactions with the heavy chain. Native Protein A contains five high affinity IgG binding domains and many other domains with unknown functions.\u003c\/p\u003e\n\u003cp\u003eThe recombinant Protein A is a genetically engineered protein that contains only the five high affinity IgG binding domains. The non-essential domains were removed to minimize nonspecific binding. The recombinant Protein A is produced in \u003ci\u003eE. coli, \u003c\/i\u003eand it essentially binds to immunoglobulins with the same degree of affinity as the native Protein A. rProtein A is capable of binding with high affinity to the Fc region from IgG of several species including human and rabbit.\u003c\/p\u003e\n\u003cp\u003eEnabled by proprietary ProteIndex™ technology, immobilization of recombinant Protein A on 4% highly cross-linked agarose resins was very stable and was optimized for high yield in laboratory preparations and scale-up applications.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eProduct Highlights\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eHigh capacity, \u0026gt; 40 mg human IgG \/ mL of settled resins\u003c\/li\u003e\n\u003cli\u003eHigh selectivity for high purity\u003c\/li\u003e\n\u003cli\u003eMechanically stable matrix support, can be used in spin (at up to 1000 g) and automated applications\u003c\/li\u003e\n\u003cli\u003eMinimal ligand leakage, can be reused\u003c\/li\u003e\n\u003cli\u003eVersatile, ready-to-use with convenient formats\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"5%\"\u003e\u003c\/td\u003e\n\u003ctd width=\"35%\"\u003e \u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/ProteinA4FF_purifying_mAscites_fluid_3_0d442212-1ce9-4a05-8106-1ec2848dd3cb.png?v=1508439312\"\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"bottom\"\u003e\n\u003cp\u003e\u003cstrong\u003eHigh purity and high capacity of rProtein A 4FF resin. \u003c\/strong\u003e2 mL mouse ascites fluid was loaded on the column containing 0.5 mL settled rProtein A 4 FF resin. Bound immunoglobulin species were eluted with 100 mM Glycine, pH 3.0. Fractions were analyzed by SDS-PAGE.\u003c\/p\u003e\n\u003cp\u003eLanes: (1) Raw ascites fluid; (2) Flow-through; (3) Elution. Note that, heavy and light chains of IgG were mostly absent from the flow-through, indicating that most immunoglobulins were bound to the column.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"200\"\u003e\u003cstrong\u003eLigand\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eRecombinant protein A with Ig binding domains\u003cem\u003e\u003cbr\u003e\u003c\/em\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBinding capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;40 mg human IgG\/mL medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMatrix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eCross-linked 4% agarose supplied as a 50% slurry\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eParticle size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e45 μm - 165 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaximum pressure\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.3 MPa (3 bar)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eChemical stability\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eAll commonly used aqueous buffers, including 6 M guanidine-HCl and 8 M urea.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eRecommended flow rate\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eGravity flow and medium pressure, 30 - 300 cm\/hour\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003epH stability\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003eLong term: pH 2 - 9;\u003c\/p\u003e\n\u003cp\u003eShort term: pH 2 - 11\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage buffer\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e20% ethanol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProduct is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e   \u003cimg style=\"float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Keep-refrigerated_95fa1867-1f0d-47e6-823a-8a0008076e21_small.png?12332456762384261517\" alt=\"Keep refrigerated\"\u003e   \u003cimg style=\"float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Do-not-freeze_2_small.png?12332456762384261517\" alt=\"Do not freeze\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eDocuments\u003c\/h5\u003e\n\u003ch4\u003eTechnical Note\u003c\/h4\u003e\n\u003cp\u003e🗎    \u003ca rel=\"noopener noreferrer\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Table_Binding_characteristics_of_immunoglobulin-binding_proteins.pdf?6231220717744860621\" title=\"Comparison of binding characteristics of immunoglobulin-binding proteins\" target=\"_blank\"\u003eComparison of Binding Characteristics of Immunoglobulin-Binding Proteins\u003c\/a\u003e\u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"5 mL","offer_id":14675386436,"sku":"11-0203-005","price":379.0,"currency_code":"USD","in_stock":true},{"title":"25 mL","offer_id":14675386500,"sku":"11-0203-025","price":1461.0,"currency_code":"USD","in_stock":true},{"title":"100 mL","offer_id":5933537263655,"sku":"11-0203-100","price":3025.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/11-0203_ProteinA4FF.png?v=1513270183"},{"product_id":"proteindex-rprotein-g-agarose-4ff","title":"PROTEINDEX™ rProtein G Agarose 4 Fast Flow","description":"\u003cp\u003e\u003cstrong\u003ePROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e\u003c\/strong\u003e™ rProtein G Agarose 4FF is an affinity chromatography medium designed for easy, one-step purification of immunoglobulins, notably most mammallian IgG isotypes, as well as Fc region-containing fusion proteins.\u003c\/p\u003e\n\u003cp\u003eProtein G is derived from \u003cem\u003eStreptococcus\u003c\/em\u003e strain G148. It contains binding sites for the Fc portion of mammalian IgG. Native protein G has 3 IgG binding domains as well as the sites for albumin and cell-surface binding. Our recombinant protein G contains only the IgG binding domains but no albumin recognition region, therefore, it is compatible with purification protocols that require the presence of fetal bovine serum (FBS) or serum albumin proteins.\u003c\/p\u003e\n\u003cp\u003eShowing super binding specificity for IgG species, \u003cstrong\u003ePROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e\u003c\/strong\u003e™ rProtein G Agarose 4FF enables single-step purification of monoclonal and polyclonal antibodies with a greater than 95% purity, and is well suited for IgG purification from various sources, including crude samples of tissue cultures and sera. With rigid, highly cross-linked matrix support, this product is versatile and well chemical-tolerant. It is readily adaptable to protocols of various formats over a broad range of chemical conditions.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eProduct Highlights\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eHigh capacity, \u0026gt; 30 mg human IgG \/ mL of settled resins\u003c\/li\u003e\n\u003cli\u003eHigh selectivity for high purity\u003c\/li\u003e\n\u003cli\u003eMechanically stable matrix support, can be used in spin (at up to 1000 g) and automated applications\u003c\/li\u003e\n\u003cli\u003eMinimal ligand leakage, can be reused\u003c\/li\u003e\n\u003cli\u003eVersatile, ready-to-use with convenient formats\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"5%\"\u003e\u003c\/td\u003e\n\u003ctd width=\"45%\"\u003e \u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/ProteinG_4FF_purifying_mAscites_fluid_2.png?v=1508441741\"\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eHigh purity and high capacity of rProtein G 4FF resin. \u003c\/strong\u003e\u003cbr\u003e2 mL mouse ascites fluid was loaded on the column containing 0.5 mL settled rProtein G 4 FF resin. Bound immunoglobulin species were eluted with 100 mM Glycine, pH 3.0. Fractions were analyzed by SDS-PAGE.\u003c\/p\u003e\n\u003cp\u003eLanes: (1) Raw ascites fluid; (2) Flow-through; (3) Elution 1; (4) Elution 2. Note that, heavy and light chains of IgG were mostly absent from the flow-through, indicating that most immunoglobulins were bound to the column.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"200\"\u003e\u003cstrong\u003eLigand\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eRecombinant protein G produced in \u003cem\u003eE.coli\u003c\/em\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBinding capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;30 mg human IgG\/mL medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMatrix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eHighly cross-linked 4% agarose supplied as 50% slurry\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eParticle size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e45 μm - 165 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaximum pressure\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.3 MPa (3 bar)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eChemical stability\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eAll commonly used aqueous buffers, including 6 M guanidine-HCl and 8 M urea.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eRecommended flow rate\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eGravity flow, 100 - 300 cm\/hour\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003epH stability\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003eLong term: pH3 - 9;\u003c\/p\u003e\n\u003cp\u003eShort term: pH2 - 10\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage buffer\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1x PBS containing 20% ethanol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProduct is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e   \u003cimg style=\"float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Keep-refrigerated_95fa1867-1f0d-47e6-823a-8a0008076e21_small.png?12332456762384261517\" alt=\"Keep refrigerated\"\u003e   \u003cimg style=\"float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Do-not-freeze_2_small.png?12332456762384261517\" alt=\"Do not freeze\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eDocuments\u003c\/h5\u003e\n\u003ch4\u003eTechnical Note\u003c\/h4\u003e\n\u003cp\u003e🗎    \u003ca rel=\"noopener noreferrer\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Table_Binding_characteristics_of_immunoglobulin-binding_proteins.pdf?6231220717744860621\" title=\"Comparison of binding characteristics of immunoglobulin-binding proteins\" target=\"_blank\"\u003eComparison of Binding Characteristics of Immunoglobulin-Binding Proteins\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"5 mL","offer_id":14872941252,"sku":"11-0206-005","price":500.0,"currency_code":"USD","in_stock":true},{"title":"25 mL","offer_id":14872941316,"sku":"11-0206-025","price":1842.0,"currency_code":"USD","in_stock":true},{"title":"100 mL","offer_id":13526966468651,"sku":"11-0206-100","price":3173.0,"currency_code":"USD","in_stock":true},{"title":"200 mL","offer_id":14872941380,"sku":"11-0206-200","price":4895.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/11-0205_ProteinG_Agarose_122d73f6-280c-4aa5-b484-b5cfd64a32fe.png?v=1493138855"},{"product_id":"proteindex-ni-penta-agarose-chemical-tolerant","title":"PROTEINDEX™ Ni-Penta™ Agarose, Chemical Stable","description":"\u003cp\u003e\u003cstrong\u003ePROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e\u003c\/strong\u003e™ \u003cstrong\u003eChemical-Tolerant Ni-Penta™ Agarose\u003c\/strong\u003e is a new immobilized metal ion affinity chromatography (IMAC) medium precharged with nickel ions, designed mainly for purification of low abundant proteins or challenging proteins in conditions where EDTA, DTT, β-ME, and other stabilizing agents are necessary for maintaining protein stability and integrity.\u003c\/p\u003e\n\u003cp\u003eThe nickel ion binds very strongly to the matrix support in complex with a proprietary pentadentate chelating ligand, which immobilizes the nickel ion through 5 sites to form a homogeneous,  chemical-stable structure. This characteristic nickel ion bonding on the Ni-Penta™ resin provides excellent resistance to EDTA, NaOH, β-ME, and other reagents that are restricted in the purification protocol using conventional NTA- and IDA- functionalized resins.\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"http:\/\/marvelgent.com\/pages\/high-selectivity-ni-penta-agarose-resins-for-secreted-proteins\" title=\"Ni-Penta resins for high selective capture of polyHis-tagged proteins\"\u003eRead more on Ni-Penta properties contributing to high selective purification of His-tagged proteins ...\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eProduct Highlights\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eEDTA-resistant\u003c\/li\u003e\n\u003cli\u003eHigh selectivity for high purity\u003c\/li\u003e\n\u003cli\u003eRetained high 6xHis affinity in various buffers\u003c\/li\u003e\n\u003cli\u003eWell suited for direct capture of secreted polyHis-tagged proteins from eukaryotic cell culture supernatant\u003c\/li\u003e\n\u003cli\u003eRecharge-free with minimal nickel ion leakage\u003c\/li\u003e\n\u003cli\u003eRequires less imidazole\u003c\/li\u003e\n\u003cli\u003eNaOH-tolerant\u003c\/li\u003e\n\u003cli\u003eVersatile, ready-to-use with convenient formats\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eApplications:\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cstrong\u003ePurification of secreted His-tagged proteins directly from eukaryotic cell culture supernatants\u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003eOwing to its proprietary ligand for nickel ion immobilization, Ni-Penta™ resin tolerates EDTA very well, and its affinity for His-tag can be retained in presence of EDTA up to 10 mM. To purify secreted proteins that are produced from eukaryotic cell cultures (such as insect High Five™ cells* and Sf9 cells, CHO cells, 293 cells etc.), a step of removing incompatible agents to Ni-NTA resins by dialysis is frequently required prior to loading the material on the column. This is tedious, time-consuming, and labor intensive. By using Ni-Penta resins, sample pretreament can be skipped, and the culture medium can be loaded directly to the column for purification.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cstrong\u003ePurification of His-tagged proteins that require presence of EDTA, DTT, β-ME, and other agents for maximum protein stability and integrity\u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003ePROTEINDEX™ Ni-Penta™ Agarose Resin is compatible with a wide range of buffers including chelating agents (e.g. EDTA) and reducing agents (e.g. DTT), which are incompatible and therefore should be avoid in purification applications using conventional NTA or IDA resins. Ni-Penta™ resin helps to simplify your sample preparation process and allows your target proteins be purified at their preferred conditions.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cstrong\u003eRepetitive uses of the column that require clean-in-place with NaOH but no nickel recharge \u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003eAnti-strip power of the Ni-Penta™ enables repetitive uses of the column without the need to recharge nickel ion, even after multiple cycles of CIP with 1 M NaOH.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cstrong\u003eRestricted imidazole amount in the elution buffer due to the requirements in downstream applications or the target protein's sensitivity to imidazole\u003cbr\u003e\u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003eImidazole is general required to minimize non-specific binding on Ni-NTA resins. Ni-Penta™ Agarose Resin is highly selective for His-tag and therefore does not demand imidazole for minimizing non-specific binding throughout purification. No imidazole is required in the binding buffer, and 40 mM to 100 mM of imidazole can efficiently elute most target proteins.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003e Technical Notes\u003c\/span\u003e\u003c\/h4\u003e\n\u003ch4\u003e 1. EDTA-stable Ni-Penta chemistry for minimal nickel ion leakage\u003c\/h4\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Ni-Penta_8_99b871bb-a5a2-4123-8891-2437bac436d6.png?v=1493146106\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eFigure 1. Strong chelate chemistry for immobilization of nickel ion. \u003c\/b\u003eNi-Penta Agarose 6 FF resins were incubated at room temperature for 2 hours in 5 volumes of a serial dilutions of EDTA from 10 mM to 400 mM. The amount of nickel ions released into the buffer was measured.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eResult:\u003c\/strong\u003e During 2 hours of incubation in 50 mM EDTA, less than 5 % of nickel ions leaked from Ni-Penta resins. In comparison, 50 mM EDTA is sufficient to strip off nickel ions from conventional Ni-NTA resins and has been used routinely to regenerate Ni-NTA resin.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e2. Uncompromised His-tag protein binding in EDTA up to 10 mM\u003c\/h4\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"color: #3d85c6;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Ni-Penta_7_ad68e7f9-16be-43e9-a619-dcfe21d2590f.png?v=1493145893\" alt=\"\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFigure 2. Retained His-tagged protein binding capacity in presence of EDTA.\u003c\/strong\u003e Ni-Penta 6FF resins were added to 20 volumes of E.coli lysate (~0.5 mg\/mL containing a His-tagged protein of 26 kDa in size), in the presence of 2 mM, 5 mM, 10 mM EDTA respectively. Protein binding was then performed at room temperature for 2 hours. After washing 5 times with 20mM sodium phosphate, 0.5 M NaCl, 5 mM imidazole, pH7.4, the target His-tagged protein was eluted with 20mM sodium phosphate, 0.5 M NaCl, 250 mM imidazole, pH7.4. Protein concentration was measured by UV absorbance at OD280.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eResult:\u003c\/strong\u003e In the presence of 10 mM EDTA, the amount of His-tag protein being purified from Ni-Penta resin was similar to those being purified in the presence of less or no EDTA.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e3. Tolerance to reducing agents\u003c\/h4\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Ni-Penta_9.png?v=1491964108\" alt=\"Ni-penta Tolerance to reducing agents\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eFigure 3. Tolerance to reducing agents. \u003c\/b\u003eNi-Penta 6FF resins were added to 20 volumes of \u003ci\u003eE.coli \u003c\/i\u003elysate (~0.5 mg\/mL containing a His-tagged protein of 26 kDa in size), in the presence of various dilutions of reducing agents, including TCEP, DTT, and β-ME respectively. Protein binding was then performed at room temperature for 2 hours. After washing 5 times with 20mM sodium phosphate, 0.5 M NaCl, 5 mM imidazole, pH7.4, the target His-tagged protein was eluted with 20mM sodium phosphate, 0.5 M NaCl, 250 mM imidazole, pH7.4. Protein concentration was measured by UV absorbance at OD280.\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eResult: \u003c\/b\u003eIn the presence of up to 5 mM TCEP, 10 mM DTT, or 50 mM β-ME, the amount of His-tag protein being purified from Ni-Penta resin was similar to those being purified in absence of the respective reducing agents.\u003c\/p\u003e\n\u003cp\u003e(A) The percentage of retained protein binding capacity is plotted against the concentration of individual reducing agents. (B) Representative SDS-PAGE analysis of the eluates.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e*\u003c\/strong\u003e High Five™ is a trade mark of Thermo Fisher Scientific.\u003c\/p\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"200\"\u003e\u003cstrong\u003eLigand\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eNickel\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBinding capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;10 mg 6XHis-tagged protein\/mL medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMatrix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e4% agarose supplied as 50% slurry\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eParticle size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e45 μm - 165 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaximum pressure\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.1 MPa (1 bar)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eRecommended flow rate\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003eGravity flow, \u0026lt;75 cm\/hour\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eChemical compatibility\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cul\u003e\n\u003cli\u003e0.01 M HCl ,0.01 M NaOH, for ≥ 1 week;\u003c\/li\u003e\n\u003cli\u003e10 mM EDTA, 1 M NaOH, 5 mM DTT, 5 mM TCEP, 20 mM β-ME, 6 M guanidine-HCl, for ≥ 24 hours\u003c\/li\u003e\n\u003cli\u003e500 mM imidazole，100 mM EDTA, for ≥ 2 hours\u003c\/li\u003e\n\u003cli\u003e30% isopropanol for ≥ 20 mins\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage buffer\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1X PBS containing 20% ethanol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProduct is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e   \u003cimg alt=\"Keep refrigerated\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Keep-refrigerated_95fa1867-1f0d-47e6-823a-8a0008076e21_small.png?12332456762384261517\" style=\"float: none;\"\u003e   \u003cimg alt=\"Do not freeze\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Do-not-freeze_2_small.png?12332456762384261517\" style=\"float: none;\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eDocuments\u003c\/h5\u003e\n\u003ch4\u003eProduct Information Sheet\u003c\/h4\u003e\n\u003cp\u003e🗎    \u003ca title=\"Man_11-0227_Chemical-Tolerant Ni-Penta Agarose_v1603.pdf\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Man_11-0227_Chemical-Tolerant_Ni-Penta_Agarose_v1603.pdf?9251540950763704712\" target=\"_blank\"\u003eNi-Penta™ Agarose\u003c\/a\u003e\u003c\/p\u003e\n\u003ch4 style=\"color: #ee8012;\"\u003e\u003cbr\u003e\u003c\/h4\u003e\n\u003cp style=\"text-align: left;\"\u003e \u003c\/p\u003e\n\u003ch5\u003eAccessories\u003c\/h5\u003e\n\u003cp\u003e\u003ca title=\"Disposable gravity flow columns\" href=\"http:\/\/marvelgent.com\/collections\/empty-gravity-flow-columns\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/Gravity_columns_730e2b1d-64df-4e61-96c2-f2f8c076c2d6_compact.png?v=1468002569\" alt=\"\"\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca title=\"Disposable gravity flow columns\" href=\"http:\/\/marvelgent.com\/collections\/empty-gravity-flow-columns\"\u003eDisposable Gravity Flow Columns\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"10 mL","offer_id":17018319684,"sku":"11-0227-010","price":146.0,"currency_code":"USD","in_stock":true},{"title":"50 mL","offer_id":17018319812,"sku":"11-0227-050","price":586.0,"currency_code":"USD","in_stock":true},{"title":"100 mL","offer_id":17018319876,"sku":"11-0227-100","price":1051.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/11-0227_Ni-Penta.png?v=1490835874"},{"product_id":"proteindex-ni-penta-agarose-6ff-chemical-tolerant","title":"PROTEINDEX™ Ni-Penta™ Agarose 6 Fast Flow, Chemical Stable","description":"\u003cp\u003e\u003cstrong\u003ePROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e\u003c\/strong\u003e™ \u003cstrong\u003eChemical-Tolerant Ni-Penta™ Agarose 6FF\u003c\/strong\u003e is a new immobilized metal ion affinity chromatography (IMAC) medium precharged with nickel ions, designed mainly for purification of low abundant proteins or challenging proteins in conditions where EDTA, DTT, β-ME, and other stabilizing agents are necessary for maintaining protein stability and integrity.\u003c\/p\u003e\n\u003cp\u003eThe nickel ion binds very strongly to the matrix support in complex with a proprietary pentadentate chelating ligand, which immobilizes the nickel ion through 5 sites to form a homogeneous,  chemical-stable structure. This characteristic nickel ion bonding on the Ni-Penta™ resin provides excellent resistance to EDTA, NaOH, β-ME, and other reagents that are restricted in the purification protocol using conventional NTA- and IDA- functionalized resins. In particular, Ni-Penta™ Agarose 6FF is well suited for direct capture of secreted polyHis-tagged proteins from the cell culture supernatant of eukaryotic cells, including CHO cells, insect High Five™ cells* and Sf9 cells.\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"http:\/\/marvelgent.com\/pages\/high-selectivity-ni-penta-agarose-resins-for-secreted-proteins\" title=\"Ni-Penta resins for high selective capture of polyHis-tagged proteins\"\u003eRead more on Ni-Penta properties contributing to high selective purification of His-tagged proteins ...\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003eHighly cross-linked 6% agarose as the base matrix support offers excellent mechanical stability for automated, scale up purification of histidine-tagged proteins.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eProduct Highlights\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eEDTA-resistant\u003c\/li\u003e\n\u003cli\u003eHigh selectivity for high purity\u003c\/li\u003e\n\u003cli\u003eRetained high 6xHis affinity in various buffers\u003c\/li\u003e\n\u003cli\u003eRecharge-free with minimal nickel ion leakage\u003c\/li\u003e\n\u003cli\u003eRequires less imidazole\u003c\/li\u003e\n\u003cli\u003eNaOH-tolerant, allowing CIP with 1 M NaOH\u003c\/li\u003e\n\u003cli\u003eVersatile, ready-to-use with convenient formats\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eApplications:\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cstrong\u003ePurification of secreted His-tagged proteins directly from eukaryotic cell culture supernatants\u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003eOwing to its proprietary ligand for nickel ion immobilization, Ni-Penta™ resin tolerates EDTA very well, and its affinity for His-tag can be retained in presence of EDTA up to 10 mM. To purify secreted proteins that are produced from eukaryotic cell cultures (such as insect cells, CHO cells, 293 cells etc.), a step of removing incompatible agents to Ni-NTA resins by dialysis is frequently required prior to loading the material on the column. This is tedious, time-consuming, and labor intensive. By using Ni-Penta resins, sample pretreament can be skipped, and the culture medium can be loaded directly to the column for purification.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cstrong\u003ePurification of His-tagged proteins that require presence of EDTA, DTT, β-ME, and other agents for maximum protein stability and integrity\u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003ePROTEINDEX™ Ni-Penta™ Agarose Resin is compatible with a wide range of buffers including chelating agents (e.g. EDTA) and reducing agents (e.g. DTT), which are incompatible and therefore should be avoid in purification applications using conventional NTA or IDA resins. Ni-Penta™ resin helps to simplify your sample preparation process and allows your target proteins be purified at their preferred conditions.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cstrong\u003eRepetitive uses of the column that require clean-in-place with NaOH but no nickel recharge \u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003eAnti-strip power of the Ni-Penta™ enables repetitive uses of the column without the need to recharge nickel ion, even after multiple cycles of CIP with 1 M NaOH.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cstrong\u003eRestricted imidazole amount in the elution buffer due to the requirements in downstream applications or the target protein's sensitivity to imidazole\u003cbr\u003e\u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003eImidazole is general required to minimize non-specific binding on Ni-NTA resins. Ni-Penta™ Agarose Resin is highly selective for His-tag and therefore does not demand imidazole for minimizing non-specific binding throughout purification. No imidazole is required in the binding buffer, and 40 mM to 100 mM of imidazole can efficiently elute most target proteins.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003e Technical Notes\u003c\/span\u003e\u003c\/h4\u003e\n\u003ch4\u003e 1. EDTA-stable Ni-Penta chemistry for minimal nickel ion leakage\u003c\/h4\u003e\n\u003cp style=\"text-align: center;\"\u003e \u003cimg alt=\"EDTA-stable chelate chemistry for immobilization of nickel ion.\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Ni-Penta_8_grande.png?v=1491963528\" style=\"float: none;\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eFigure 1. Strong chelate chemistry for immobilization of nickel ion. \u003c\/b\u003e\u003c\/p\u003e\n\u003cp\u003eNi-Penta Agarose 6 FF resins were incubated at room temperature for 2 hours in 5 volumes of a serial dilutions of EDTA from 10 mM to 400 mM. The amount of nickel ions released into the buffer was measured.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eResult:\u003c\/strong\u003e During 2 hours of incubation in 50 mM EDTA, less than 5 % of nickel ions leaked from Ni-Penta resins. In comparison, 50 mM EDTA is sufficient to strip off nickel ions from conventional Ni-NTA resins and has been used routinely to regenerate Ni-NTA resin.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e2. Uncompromised His-tag protein binding in EDTA up to 10 mM\u003c\/h4\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"color: #3d85c6;\"\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Ni-Penta_7_654f089b-e6ea-413d-9b06-abb3ead360a8_grande.png?v=1491962523\" style=\"float: none;\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFigure 2. Retained His-tagged protein binding capacity in presence of EDTA.\u003c\/strong\u003e Ni-Penta 6FF resins were added to 20 volumes of E.coli lysate (~0.5 mg\/mL containing a His-tagged protein of 26 kDa in size), in the presence of 2 mM, 5 mM, 10 mM EDTA respectively. Protein binding was then performed at room temperature for 2 hours. After washing 5 times with 20mM sodium phosphate, 0.5 M NaCl, 5 mM imidazole, pH7.4, the target His-tagged protein was eluted with 20mM sodium phosphate, 0.5 M NaCl, 250 mM imidazole, pH7.4. Protein concentration was measured by UV absorbance at OD280.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eResult:\u003c\/strong\u003e In the presence of 10 mM EDTA, the amount of His-tag protein being purified from Ni-Penta resin was similar to those being purified in the presence of less or no EDTA.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e3. Tolerance to reducing agents\u003c\/h4\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Ni-Penta_9.png?v=1491964108\" alt=\"Ni-penta Tolerance to reducing agents\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eFigure 3. Tolerance to reducing agents. \u003c\/b\u003e\u003c\/p\u003e\n\u003cp\u003eNi-Penta 6FF resins were added to 20 volumes of \u003ci\u003eE.coli \u003c\/i\u003e lysate (~0.5 mg\/mL containing a His-tagged protein of 26 kDa in size), in the presence of various dilutions of reducing agents, including TCEP, DTT, and β-ME respectively. Protein binding was then performed at room temperature for 2 hours. After washing 5 times with 20mM sodium phosphate, 0.5 M NaCl, 5 mM imidazole, pH7.4, the target His-tagged protein was eluted with 20mM sodium phosphate, 0.5 M NaCl, 250 mM imidazole, pH7.4. Protein concentration was measured by UV absorbance at OD280.\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eResult: \u003c\/b\u003eIn the presence of up to 5 mM TCEP, 10 mM DTT, or 50 mM β-ME, the amount of His-tag protein being purified from Ni-Penta resin was similar to those being purified in absence of the respective reducing agents.\u003c\/p\u003e\n\u003cp\u003e(A) The percentage of retained protein binding capacity is plotted against the concentration of individual reducing agents. (B) Representative SDS-PAGE analysis of the eluates.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e*\u003c\/strong\u003e High Five™ is a trade mark of Thermo Fisher Scientific.\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"200\"\u003e\u003cstrong\u003eLigand\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eNickel\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBinding capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;10 mg 6XHis-tagged protein\/mL medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMetal ion capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u0026gt; 50 µmol Ni\u003csup\u003e2+ \u003c\/sup\u003e\/ ml medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMatrix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eHighly cross-linked 6% agarose resin supplied as 50% slurry\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eParticle size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e45 μm - 165 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaximum pressure\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.3 MPa (3 bar)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eChemical compatibility\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cul\u003e\n\u003cli\u003e0.01 M HCl ,0.01 M NaOH, for ≥ 1 week;\u003c\/li\u003e\n\u003cli\u003e10 mM EDTA, 1 M NaOH, 5 mM DTT, 5 mM TCEP, 20 mM β-ME, 6 M guanidine-HCl, for ≥ 24 hours\u003c\/li\u003e\n\u003cli\u003e500 mM imidazole，100 mM EDTA, for ≥ 2 hours\u003c\/li\u003e\n\u003cli\u003e30% isopropanol for ≥ 20 mins\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage buffer\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1X PBS containing 20% ethanol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProduct is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e   \u003cimg alt=\"Keep refrigerated\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Keep-refrigerated_95fa1867-1f0d-47e6-823a-8a0008076e21_small.png?12332456762384261517\" style=\"float: none;\"\u003e   \u003cimg alt=\"Do not freeze\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Do-not-freeze_2_small.png?12332456762384261517\" style=\"float: none;\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eDocuments\u003c\/h5\u003e\n\u003ch4\u003eProduct Information Sheet\u003c\/h4\u003e\n\u003cp\u003e🗎    \u003ca title=\"Man_11-0228_Chemical-Tolerant Ni-Penta Agarose6FF_v1603.pdf\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Man_11-0228_Chemical-Tolerant_Ni-Penta_Agarose_6_Fast_Flow_v1603.pdf?4531069748310472707\" target=\"_blank\"\u003eNi-Penta™ Agarose 6FF\u003c\/a\u003e\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"10 mL","offer_id":17033312580,"sku":"11-0228-010","price":146.0,"currency_code":"USD","in_stock":true},{"title":"50 mL","offer_id":17033312644,"sku":"11-0228-050","price":586.0,"currency_code":"USD","in_stock":true},{"title":"100 mL","offer_id":17033312708,"sku":"11-0228-100","price":1051.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/Ni-Penta.png?v=1483497352"},{"product_id":"proteindex-ni-penta-agarose-6-fast-flow-chemical-stable-br-prepacked-cartridge","title":"PROTEINDEX™ Ni-Penta™ Agarose 6 Fast Flow, Chemical Stable, Prepacked Cartridge","description":"\u003cp\u003e\u003cstrong\u003eROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e\u003c\/strong\u003e™ \u003cstrong\u003eChemical-Tolerant Ni-Penta™ Agarose 6FF\u003c\/strong\u003e is a new immobilized metal ion affinity chromatography (IMAC) medium precharged with nickel ions, designed mainly for purification of low abundant proteins or challenging proteins in conditions where EDTA, DTT, β-ME, and other stabilizing agents are necessary for maintaining protein stability and integrity.\u003c\/p\u003e\n\u003cp\u003eThe nickel ion binds very strongly to the matrix support in complex with a proprietary pentadentate chelating ligand, which immobilizes the nickel ion through 5 sites to form a homogeneous,  chemical-stable structure. This characteristic nickel ion bonding on the Ni-Penta™ resin provides excellent resistance to EDTA, NaOH, β-ME, and other reagents that are restricted in the purification protocol using conventional NTA- and IDA- functionalized resins. In particular, Ni-Penta™ Agarose 6FF is well suited for direct capture of secreted polyHis-tagged proteins from the cell culture supernatant of eukaryotic cells, including CHO cells, insect High Five™ cells* and Sf9 cells.\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"http:\/\/marvelgent.com\/pages\/high-selectivity-ni-penta-agarose-resins-for-secreted-proteins\" title=\"Ni-Penta resins for high selective capture of polyHis-tagged proteins\"\u003eRead more on Ni-Penta properties contributing to high selective purification of His-tagged proteins ...\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003eNi-Penta™ Agarose 6 FF Prepacked Cartridge are packed with 1 ml and 5 ml of Ni-Penta Agarose 6 Fast Flow. The columns have standard interface that can be adapted to most commonly used chromatography systems, such as ÄKTA.\u003c\/p\u003e\n\u003cp\u003ePrepacked 16\/10 columns and 7\/10 columns offer a bed height of 10 cm that is convenient for process scale-up and development.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eValue bundle with a \u003ca rel=\"noopener\" title=\"Connector Luer Female \/ 10-32 Male in PEEK\" href=\"https:\/\/marvelgent.com\/products\/connector-1-16-luer-female-10-32-male-peek\" target=\"_blank\"\u003eLuer Female\/10-32 Male Connector in PEEK\u003c\/a\u003e (Cat# 20-0315) is now available for all pack sizes, providing convenient operation of the pre-packed column with a syringe at an excellent value.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eProduct Highlights\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eEDTA-resistant\u003c\/li\u003e\n\u003cli\u003eHigh selectivity for high purity\u003c\/li\u003e\n\u003cli\u003eRetained high 6xHis affinity in various buffers\u003c\/li\u003e\n\u003cli\u003eRecharge-free with minimal nickel ion leakage\u003c\/li\u003e\n\u003cli\u003eRequires less imidazole\u003c\/li\u003e\n\u003cli\u003eNaOH-tolerant, allowing CIP with 1 M NaOH\u003c\/li\u003e\n\u003cli\u003eReady-to-use, convenient formats: 1 mL and 5 mL\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eApplications:\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cstrong\u003ePurification of secreted His-tagged proteins directly from eukaryotic cell culture supernatants\u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003eOwing to its proprietary ligand for nickel ion immobilization, Ni-Penta™ resin tolerates EDTA very well, and its affinity for His-tag can be retained in presence of EDTA up to 10 mM. To purify secreted proteins that are produced from eukaryotic cell cultures (such as insect cells, CHO cells, 293 cells etc.), a step of removing incompatible agents to Ni-NTA resins by dialysis is frequently required prior to loading the material on the column. This is tedious, time-consuming, and labor intensive. By using Ni-Penta resins, sample pretreament can be skipped, and the culture medium can be loaded directly to the column for purification.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cstrong\u003ePurification of His-tagged proteins that require presence of EDTA, DTT, β-ME, and other agents for maximum protein stability and integrity\u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003ePROTEINDEX™ Ni-Penta™ Agarose Resin is compatible with a wide range of buffers including chelating agents (e.g. EDTA) and reducing agents (e.g. DTT), which are incompatible and therefore should be avoid in purification applications using conventional NTA or IDA resins. Ni-Penta™ resin helps to simplify your sample preparation process and allows your target proteins be purified at their preferred conditions.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cstrong\u003eRepetitive uses of the column that require clean-in-place with NaOH but no nickel recharge \u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003eAnti-strip power of the Ni-Penta™ enables repetitive uses of the column without the need to recharge nickel ion, even after multiple cycles of CIP with 1 M NaOH.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cstrong\u003eRestricted imidazole amount in the elution buffer due to the requirements in downstream applications or the target protein's sensitivity to imidazole\u003cbr\u003e\u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003eImidazole is general required to minimize non-specific binding on Ni-NTA resins. Ni-Penta™ Agarose Resin is highly selective for His-tag and therefore does not demand imidazole for minimizing non-specific binding throughout purification. No imidazole is required in the binding buffer, and 40 mM to 100 mM of imidazole can efficiently elute most target proteins.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\n\u003cspan style=\"color: #3d85c6;\"\u003e Technical Notes\u003c\/span\u003e \u003c\/h4\u003e\n\u003ch4\u003e1. EDTA-stable Ni-Penta chemistry for minimal nickel ion leakage\u003c\/h4\u003e\n\u003cp style=\"text-align: center;\"\u003e \u003cimg alt=\"EDTA-stable chelate chemistry for immobilization of nickel ion.\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Ni-Penta_8_grande.png?v=1491963528\" style=\"float: none;\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eFigure 1. Strong chelate chemistry for immobilization of nickel ion. \u003c\/b\u003eNi-Penta Agarose 6 FF resins were incubated at room temperature for 2 hours in 5 volumes of a serial dilutions of EDTA from 10 mM to 400 mM. The amount of nickel ions released into the buffer was measured.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eResult:\u003c\/strong\u003e During 2 hours of incubation in 50 mM EDTA, less than 5 % of nickel ions leaked from Ni-Penta resins. In comparison, 50 mM EDTA is sufficient to strip off nickel ions from conventional Ni-NTA resins and has been used routinely to regenerate Ni-NTA resin.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e2. Uncompromised His-tag protein binding in EDTA up to 10 mM\u003c\/h4\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"color: #3d85c6;\"\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Ni-Penta_7_654f089b-e6ea-413d-9b06-abb3ead360a8_grande.png?v=1491962523\" style=\"float: none;\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFigure 2. Retained His-tagged protein binding capacity in presence of EDTA.\u003c\/strong\u003e Ni-Penta 6FF resins were added to 20 volumes of E.coli lysate (~0.5 mg\/mL containing a His-tagged protein of 26 kDa in size), in the presence of 2 mM, 5 mM, 10 mM EDTA respectively. Protein binding was then performed at room temperature for 2 hours. After washing 5 times with 20mM sodium phosphate, 0.5 M NaCl, 5 mM imidazole, pH7.4, the target His-tagged protein was eluted with 20mM sodium phosphate, 0.5 M NaCl, 250 mM imidazole, pH7.4. Protein concentration was measured by UV absorbance at OD280.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eResult:\u003c\/strong\u003e In the presence of 10 mM EDTA, the amount of His-tag protein being purified from Ni-Penta resin was similar to those being purified in the presence of less or no EDTA.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e3. Tolerance to reducing agents\u003c\/h4\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Ni-Penta_9.png?v=1491964108\" alt=\"Ni-penta Tolerance to reducing agents\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eFigure 3. Tolerance to reducing agents. \u003c\/b\u003eNi-Penta 6FF resins were added to 20 volumes of \u003ci\u003eE.coli \u003c\/i\u003elysate (~0.5 mg\/mL containing a His-tagged protein of 26 kDa in size), in the presence of various dilutions of reducing agents, including TCEP, DTT, and β-ME respectively. Protein binding was then performed at room temperature for 2 hours. After washing 5 times with 20mM sodium phosphate, 0.5 M NaCl, 5 mM imidazole, pH7.4, the target His-tagged protein was eluted with 20mM sodium phosphate, 0.5 M NaCl, 250 mM imidazole, pH7.4. Protein concentration was measured by UV absorbance at OD280.\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eResult: \u003c\/b\u003eIn the presence of up to 5 mM TCEP, 10 mM DTT, or 50 mM β-ME, the amount of His-tag protein being purified from Ni-Penta resin was similar to those being purified in absence of the respective reducing agents.\u003c\/p\u003e\n\u003cp\u003e(A) The percentage of retained protein binding capacity is plotted against the concentration of individual reducing agents. (B) Representative SDS-PAGE analysis of the eluates.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e*\u003c\/strong\u003e High Five™ is a trade mark of Thermo Fisher Scientific.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"200\"\u003e\u003cstrong\u003eLigand\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eNickel\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBinding capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;10 mg 6XHis-tagged protein\/mL medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMatrix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eHighly cross-linked 6% agarose resin\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eParticle size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e45 μm - 165 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaximum pressure\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.3 MPa (3 bar)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eChemical compatibility\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cul\u003e\n\u003cli\u003e0.01 M HCl ,0.01 M NaOH, for ≥ 1 week;\u003c\/li\u003e\n\u003cli\u003e10 mM EDTA, 1 M NaOH, 5 mM DTT, 5 mM TCEP, 20 mM β-ME, 6 M guanidine-HCl, for ≥ 24 hours\u003c\/li\u003e\n\u003cli\u003e500 mM imidazole，100 mM EDTA, for ≥ 2 hours\u003c\/li\u003e\n\u003cli\u003e30% isopropanol for ≥ 20 mins\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage buffer\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1x PBS containing 20% ethanol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProduct is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e   \u003cimg alt=\"Keep refrigerated\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Keep-refrigerated_95fa1867-1f0d-47e6-823a-8a0008076e21_small.png?12332456762384261517\" style=\"float: none;\"\u003e   \u003cimg alt=\"Do not freeze\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Do-not-freeze_2_small.png?12332456762384261517\" style=\"float: none;\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eDocuments\u003c\/h5\u003e\n\u003ch4\u003eProduct Information Sheet\u003c\/h4\u003e\n\u003cp\u003e🗎    \u003ca title=\"Man_11-0228_Chemical-Tolerant Ni-Penta Agarose6FF Prepacked Cartridges_v1603.pdf\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Man_11-0229_Chemical-Tolerant_Ni-Penta_Agarose_6_FF_Prepacked_Cartridge_v1603.pdf?9251540950763704712\" rel=\"noopener noreferrer\" target=\"_blank\"\u003eNi-Penta™ Agarose 6FF, Prepacked Cartridges\u003c\/a\u003e\u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"1 mL Columns, Pack of 5","offer_id":41405565042731,"sku":"11-0229-5x1ML","price":177.0,"currency_code":"USD","in_stock":true},{"title":"1 mL Columns, Pack of 5 with 1 Connector","offer_id":41405559537707,"sku":"11-0229-5x1MLB","price":204.0,"currency_code":"USD","in_stock":true},{"title":"5 mL Column, Pack of 1","offer_id":41405388554283,"sku":"11-0229-1x5ML","price":151.0,"currency_code":"USD","in_stock":true},{"title":"5 mL Column, Pack of 1,  with 1 Connector","offer_id":41405562585131,"sku":"11-0229-1x5MLB","price":179.0,"currency_code":"USD","in_stock":true},{"title":"5 mL Columns, Pack of 5","offer_id":41405565173803,"sku":"11-0229-5x5ML","price":586.0,"currency_code":"USD","in_stock":true},{"title":"5 mL Columns, Pack of 5, with 1 Connector","offer_id":41405388619819,"sku":"11-0229-5x5MLB","price":613.0,"currency_code":"USD","in_stock":true},{"title":"16\/10 Column, 20 mL","offer_id":42112519569451,"sku":"11-0229-C20","price":709.0,"currency_code":"USD","in_stock":true},{"title":"7\/10 Columns, 4.7 mL, Pack of 2","offer_id":42112598048811,"sku":"11-0229-C47","price":544.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/11-0229_Ni-Penta_cartridges.png?v=1493997900"},{"product_id":"proteindex-ni-nta-agarose","title":"PROTEINDEX™ HiBond™ Ni-NTA Agarose","description":"\u003cp\u003e\u003cstrong\u003ePROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e™ HiBond™ Ni-NTA Agarose \u003c\/strong\u003eutilizes 4% agarose as the matrix support, and is functionalized with the chelate ligand Nitilotriacetic Acid (NTA), which is charged with nickel ions (Ni\u003csup\u003e2+\u003c\/sup\u003e) to form a very stable octahedral structure with the nickel ion in the center. The structure of Ni-NTA complex is compatible with solutions that contain certain concentration of reducing agents, denaturing agents, detergents and other additives.\u003c\/p\u003e\n\u003cp\u003ePROTEINDEX™ HiBond™ Ni-NTA Agarose can be used to purify 6xHis-tagged proteins expressed in various hosts, such as\u003cem\u003e E.coli\u003c\/em\u003e., yeast, insect cells and mammalian cells.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eProduct Highlights\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eSuited for routine purification of histidine-tagged proteins\u003c\/li\u003e\n\u003cli\u003eHigh yield (typically \u0026gt; 40 mg protein \/mL resins)\u003c\/li\u003e\n\u003cli\u003eLow leakage of nickel ions. Can be re-used for up to 8 preps*\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cspan style=\"color: #666666;\"\u003e*Note: No significant decrease in yield after 8 repetitive uses of resin when recommended buffers are used. Refer to Product Details for compatible reagents.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eApplications\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eManual batch or gravity flow purification\u003c\/li\u003e\n\u003cli\u003eRoutine His tag protein purification for structural biology and functional assays\u003c\/li\u003e\n\u003cli\u003eProtein-protein and protein-nucleic acid interaction studies\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003e\u003c\/span\u003e\u003c\/h4\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eTech Notes\u003c\/span\u003e\u003c\/h4\u003e\n\u003cp\u003e\u003cb\u003eOne-step purification using \u003c\/b\u003e\u003cb\u003eP\u003c\/b\u003e\u003cb\u003eroteIndex\u003c\/b\u003e\u003cb\u003e™ \u003c\/b\u003e\u003cb\u003eHiBond\u003c\/b\u003e\u003cb\u003e™ Ni-NTA \u003c\/b\u003e\u003cb\u003eAgarose 6 Fast Flow resins \u003c\/b\u003e\u003c\/p\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 0px; border-color: #ffffff;\" width=\"30%\"\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Ni-NTA_2.png?v=1496932129\" alt=\"\"\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 0px; border-color: #ffffff;\" width=\"30%\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cstrong\u003eFigure 1. \u003c\/strong\u003eE.coli lysate containing a 50 kDa target protein was directly loaded on HiBond™ Ni-NTA Agarose 6 FF column. The column was then washed sequentially with 0 mM, 5 mM, and 20 mM imidazole solution before elution with 300 mM imidazole. The target protein was analyzed by SDS-PAGE. \u003cb\u003eC\u003c\/b\u003e\u003cb\u003eL\u003c\/b\u003e: raw lysate; \u003cb\u003eFT\u003c\/b\u003e: unbound flow-through; \u003cb\u003eW1\u003c\/b\u003e,\u003cb\u003e W2 \u003c\/b\u003eand \u003cb\u003eW3: \u003c\/b\u003ewashes; \u003cb\u003eE1\u003c\/b\u003e and \u003cb\u003eE2\u003c\/b\u003e: eluate fractions.\n\u003cul\u003e\u003c\/ul\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"200\"\u003e\u003cstrong\u003eLigand\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eNickel\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBinding capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;40 mg 6xHis-tagged protein\/mL medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMatrix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e4% agarose supplied as 50% slurry\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eParticle size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e45 μm - 165 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaximum pressure\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.1 MPa (1 bar)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eRecommended flow rate\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003eGravity flow, \u0026lt;75 cm\/hour\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eChemical compatibility\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003ctable width=\"93%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"37%\" valign=\"top\"\u003eReducing agents\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e5 mM DTE\u003cbr\u003e0.5-1mM DTT\u003cbr\u003e20 mM β-mercaptoethanol\u003cbr\u003e5 mM TCEP\u003cbr\u003e10 mM reduced glutathione (GSH)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"37%\" valign=\"top\"\u003eDenaturing agents\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e8 M urea, 6 M Gua-HCl \u003cbr\u003e(Tested for one week at 40°C)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"37%\" valign=\"top\"\u003eDetergents\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e2% Triton™ X-100 (nonionic)\u003cbr\u003e2% Tween™ 20 (nonionic)\u003cbr\u003e2% NP-40 (nonionic)\u003cbr\u003e2% cholate (anionic)\u003cbr\u003e1% CHAPS (zwitterionic)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"37%\" valign=\"top\"\u003eOther additives\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e500 mM imidazole\u003cbr\u003e20% ethanol\u003cbr\u003e50% glycerol\u003cbr\u003e100 mM Na\u003csub\u003e2\u003c\/sub\u003eSO4\u003cbr\u003e1.5 M NaCl\u003cbr\u003e1 mM EDTA\u003cbr\u003e60 mM citrate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"37%\" valign=\"top\"\u003eCommonly used buffer\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e50 mM sodium phosphate, pH 7.4\u003cbr\u003e100 mM Tris-HCl, pH 7.4\u003cbr\u003e100 mM Tris-acetate, pH 7.4\u003cbr\u003e100 mM HEPES, pH 7.4\u003cbr\u003e100 mM MOPS, pH 7.4\u003cbr\u003e100 mM sodium acetate, pH 4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage buffer\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1x PBS containing 20% ethanol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProduct is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e   \u003cimg alt=\"Keep refrigerated\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Keep-refrigerated_95fa1867-1f0d-47e6-823a-8a0008076e21_small.png?12332456762384261517\" style=\"float: none;\"\u003e   \u003cimg alt=\"Do not freeze\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Do-not-freeze_2_small.png?12332456762384261517\" style=\"float: none;\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003e\u003c\/h5\u003e\n\u003ch4 style=\"color: #ee8012;\"\u003e\u003c\/h4\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"10 mL","offer_id":18927593668,"sku":"11-0224-010","price":115.0,"currency_code":"USD","in_stock":true},{"title":"50 mL","offer_id":18927593732,"sku":"11-0224-050","price":412.0,"currency_code":"USD","in_stock":true},{"title":"100 mL","offer_id":18927593796,"sku":"11-0224-100","price":759.0,"currency_code":"USD","in_stock":true},{"title":"500 mL","offer_id":39291740979243,"sku":"11-0224-500","price":2733.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/11-0224_Ni-NTA.png?v=1483498777"},{"product_id":"proteindex-ni-nta-agarose-6ff","title":"PROTEINDEX™ HiBond™ Ni-NTA Agarose 6FF","description":"\u003cp\u003e\u003cstrong\u003ePROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e\u003c\/strong\u003e™ \u003cb\u003eHiBond\u003c\/b\u003e\u003cb\u003e™ \u003c\/b\u003e\u003cstrong\u003eNi-NTA Agarose\u003c\/strong\u003e \u003cstrong\u003e6FF\u003c\/strong\u003e has been optimized for automated purification applications. It has been widely used in scale-up and preparative purification of histidine-tagged proteins that are expressed in various hosts, such as \u003cem\u003eE.coli\u003c\/em\u003e, yeast, insect cells, and mammalian cells.\u003c\/p\u003e\n\u003cp\u003e        The base matrix of PROTEINDEX™ HiBond™ Ni-NTA Agarose 6FF is highly crosslinked 6% agarose, offering excellent chemical-, and mechanical stability for automated applications. The matrix support is functionalized with the chelate ligand Nitilotriacetic Acid (NTA) in complex with the nickel ions (Ni\u003csup\u003e2+\u003c\/sup\u003e) in a very stable octahedral structure. The structure of Ni-NTA complex is compatible solutions that contain certain concentration of reducing agents, denaturing agents, detergents and other additives.\u003c\/p\u003e\n\u003cp\u003e        The NTA ligand coordinates the Ni\u003csup\u003e2+\u003c\/sup\u003e with four valencies (tetradentate, coordination number 4), and two valencies are available for interaction with imidazole rings of histidine residues. The number of available valencies seems to provide the best combination of binding effectiveness and chemical stability for binding with the His tag. The Ni-NTA ion-chelate complex on our ProteIndex™ HiBond™ Ni-NTA resins has been delicately optimized to ensure the most effective capture for His tag purification of His-tagged proteins. \u003c\/p\u003e\n\u003cp\u003e       \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eProduct Highlights\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eMechanical stable, designed for scaling up His tag purification\u003c\/li\u003e\n\u003cli\u003eHigh yield (typically \u0026gt; 40 mg protein \/mL resins)\u003c\/li\u003e\n\u003cli\u003eLow leakage of nickel ions. Can be re-used for up to 8 preps*\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cspan style=\"color: #666666;\"\u003e*Note: No significant decrease in yield after 8 repetitive uses of resin when recommended buffers are used. Refer to Product Details for compatible reagents.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eApplications\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eAutomated or syringe operations; and manual batch or gravity flow\u003c\/li\u003e\n\u003cli\u003eRoutine His tag protein purification for structural biology and functional assays\u003c\/li\u003e\n\u003cli\u003eScaling up and preparative applications\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eTech Notes\u003c\/span\u003e\u003c\/h4\u003e\n\u003cp\u003e\u003cb\u003eOne-step purification using \u003c\/b\u003e\u003cb\u003eP\u003c\/b\u003e\u003cb\u003eroteIndex\u003c\/b\u003e\u003cb\u003e™ \u003c\/b\u003e\u003cb\u003eHiBond\u003c\/b\u003e\u003cb\u003e™ Ni-NTA \u003c\/b\u003e\u003cb\u003eAgarose 6 Fast Flow resins \u003c\/b\u003e\u003c\/p\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 0px; border-color: #ffffff;\" width=\"35%\"\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Ni-NTA_6FF_2.png?v=1496201543\" alt=\"\" style=\"display: block; margin-left: auto; margin-right: auto;\"\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 0px; border-color: #ffffff;\" width=\"35%\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cstrong\u003eFigure 1.\u003c\/strong\u003e A 15 kDa protein was expressed in E.coli and purified on an automated system using \u003cb\u003eP\u003c\/b\u003e\u003cb\u003eroteIndex\u003c\/b\u003e\u003cb\u003e™ \u003c\/b\u003e\u003cb\u003eHiBond\u003c\/b\u003e\u003cb\u003e™ Ni-NTA \u003c\/b\u003e\u003cb\u003eAgarose 6 Fast Flow resin \u003c\/b\u003eunder native conditions. 50 mL E.coli lysate in 50 mM NaH\u003csub\u003e2\u003c\/sub\u003ePO\u003csub\u003e4\u003c\/sub\u003e, 0.3 M NaCl, 10 mM imidazole was loaded on the column packed with 0.5 mL resins at the speed of 1 mL\/min. The column was then washed with 100 mL 50 mM NaH\u003csub\u003e2\u003c\/sub\u003ePO\u003csub\u003e4\u003c\/sub\u003e, 0.3 M NaCl, 20 mM imidazole at the speed of 1~2 mL\/min. The target protein was eluted with 50 mM NaH\u003csub\u003e2\u003c\/sub\u003ePO\u003csub\u003e4\u003c\/sub\u003e, 0.3 M NaCl, 250 mM imidazole. Proteins were visualized by coomassie blue staining after separation on SDS-PAGE. \u003cb\u003eL\u003c\/b\u003e: raw lysate; \u003cb\u003eF\u003c\/b\u003e: unbound flow-through; \u003cb\u003eE1\u003c\/b\u003e and \u003cb\u003eE2\u003c\/b\u003e: eluate fractions.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 0px; border-color: #ffffff;\" width=\"37%\"\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Ni-NTA_6FF_comparison_with_Ni_sepharose_4.png?v=1497317795\" alt=\"\"\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 0px; border-color: #ffffff;\" width=\"37%\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cstrong\u003eFigure 2.\u003c\/strong\u003e A 25 kDa protein was expressed in E.coli and purified on an automated system using a one-step automated protocol with \u003cb\u003eP\u003c\/b\u003e\u003cb\u003eroteIndex\u003c\/b\u003e\u003cb\u003e™ \u003c\/b\u003e\u003cb\u003eHiBond\u003c\/b\u003e\u003cb\u003e™ Ni-NTA \u003c\/b\u003e\u003cb\u003eAgarose 6 Fast Flow resin \u003c\/b\u003eunder native conditions. 50 mL E.coli lysate in 50 mM NaH\u003csub\u003e2\u003c\/sub\u003ePO\u003csub\u003e4\u003c\/sub\u003e, 0.3 M NaCl, 10 mM imidazole was loaded on the column packed with 0.5 mL resins at the speed of 1 mL\/min. The column was then washed with 100 mL 50 mM NaH\u003csub\u003e2\u003c\/sub\u003ePO\u003csub\u003e4\u003c\/sub\u003e, 0.3 M NaCl, 20 mM imidazole at the speed of 1~2 mL\/min. The target protein was eluted with 50 mM NaH\u003csub\u003e2\u003c\/sub\u003ePO\u003csub\u003e4\u003c\/sub\u003e, 0.3 M NaCl, 250 mM imidazole. A fraction of eluate was analyzed on a SDS-PAGE. Shown were coomassie blue stains of the target proteins purified from columns of Marvelgent’s ProteIndex Ni-NTA 6FF (\u003cstrong\u003eM\u003c\/strong\u003e) and Ni-Sepharose (\u003cstrong\u003eG\u003c\/strong\u003e) resins.\u003cbr\u003e\u003c\/p\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"200\"\u003e\u003cstrong\u003eLigand\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eNickel\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBinding capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u0026gt; 40 mg 6xHis-tagged protein\/mL medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMatrix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e6% highly cross-linked agarose supplied as 50% slurry\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eParticle size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e45 μm - 165 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaximum pressure\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.3 MPa (3 bar)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eChemical compatibility\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003ctable width=\"93%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"37%\" valign=\"top\"\u003eReducing agents\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e5 mM DTE\u003cbr\u003e0.5-1 mM DTT\u003cbr\u003e20 mM β-mercaptoethanol\u003cbr\u003e5 mM TCEP\u003cbr\u003e10 mM reduced glutathione (GSH)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"37%\" valign=\"top\"\u003eDenaturing agents\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e8 M urea, 6 M Gua-HCl \u003cbr\u003e(Tested for one week at 40°C)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"37%\" valign=\"top\"\u003eDetergents\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e2% Triton™ X-100 (nonionic)\u003cbr\u003e2% Tween™ 20 (nonionic)\u003cbr\u003e2% NP-40 (nonionic)\u003cbr\u003e2% cholate (anionic)\u003cbr\u003e1% CHAPS (zwitterionic)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"37%\" valign=\"top\"\u003eOther additives\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e500 mM imidazole\u003cbr\u003e20% ethanol\u003cbr\u003e50% glycerol\u003cbr\u003e100 mM Na\u003csub\u003e2\u003c\/sub\u003eSO4\u003cbr\u003e1.5 M NaCl\u003cbr\u003e1 mM EDTA\u003cbr\u003e60 mM citrate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"37%\" valign=\"top\"\u003eCommonly used buffer\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e50 mM sodium phosphate, pH 7.4\u003cbr\u003e100 mM Tris-HCl, pH 7.4\u003cbr\u003e100 mM Tris-acetate, pH 7.4\u003cbr\u003e100 mM HEPES, pH 7.4\u003cbr\u003e100 mM MOPS, pH 7.4\u003cbr\u003e100 mM sodium acetate, pH 4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage buffer\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1x PBS containing 20% ethanol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProduct is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e   \u003cimg alt=\"Keep refrigerated\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Keep-refrigerated_95fa1867-1f0d-47e6-823a-8a0008076e21_small.png?12332456762384261517\" style=\"float: none;\"\u003e   \u003cimg alt=\"Do not freeze\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Do-not-freeze_2_small.png?12332456762384261517\" style=\"float: none;\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003e\u003cbr\u003e\u003c\/h5\u003e\n\u003ch4 style=\"color: #ee8012;\"\u003e\u003cbr\u003e\u003c\/h4\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"10 mL","offer_id":19000297540,"sku":"11-0225-010","price":126.0,"currency_code":"USD","in_stock":true},{"title":"50 mL","offer_id":19000297604,"sku":"11-0225-050","price":506.0,"currency_code":"USD","in_stock":true},{"title":"100 mL","offer_id":19000297668,"sku":"11-0225-100","price":825.0,"currency_code":"USD","in_stock":true},{"title":"500 mL","offer_id":5704109129767,"sku":"11-0225-500","price":3366.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/11-0225_Ni-NTA_6FF.png?v=1496933028"},{"product_id":"proteindex-ni-nta-agarose-6ff-prepacked-cartridge","title":"PROTEINDEX™ HiBond™ Ni-NTA Agarose 6FF, Prepacked Cartridge","description":"\u003cp\u003e\u003cstrong\u003ePROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e\u003c\/strong\u003e™ \u003cb\u003eHiBond\u003c\/b\u003e\u003cb\u003e™ \u003c\/b\u003e\u003cstrong\u003eNi-NTA Agarose\u003c\/strong\u003e \u003cstrong\u003e6FF\u003c\/strong\u003e has been optimized for automated purification applications. It has been widely used in scale-up and preparative purification of histidine-tagged proteins that are expressed in various hosts, such as \u003cem\u003eE.coli\u003c\/em\u003e, yeast, insect cells, and mammalian cells.\u003c\/p\u003e\n\u003cp\u003e        The base matrix of PROTEINDEX™ HiBond™ Ni-NTA Agarose 6FF is highly crosslinked 6% agarose, offering excellent chemical-, and mechanical stability for automated applications. The matrix support is functionalized with the chelate ligand Nitilotriacetic Acid (NTA) in complex with the nickel ions (Ni\u003csup\u003e2+\u003c\/sup\u003e) in a very stable octahedral structure. The structure of Ni-NTA complex is compatible solutions that contain certain concentration of reducing agents, denaturing agents, detergents and other additives.\u003c\/p\u003e\n\u003cp\u003e        The NTA ligand coordinates the Ni\u003csup\u003e2+\u003c\/sup\u003e with four valencies (tetradentate, coordination number 4), and two valencies are available for interaction with imidazole rings of histidine residues. The number of available valencies seems to provide the best combination of binding effectiveness and chemical stability for binding with the His tag. The Ni-NTA ion-chelate complex on our ProteIndex™ HiBond™ Ni-NTA resins has been delicately optimized to ensure the most effective capture for His tag purification of His-tagged proteins. \u003c\/p\u003e\n\u003cp\u003e        The Prepacked Cartridge has standard interface and can be adapted to most commonly used chromatography systems, such as ÄKTA systems.\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e        Value bundle with a \u003c\/span\u003e\u003ca rel=\"noopener\" title=\"Connector Luer Female \/ 10-32 Male in PEEK\" href=\"https:\/\/marvelgent.com\/products\/connector-1-16-luer-female-10-32-male-peek\" target=\"_blank\"\u003eLuer Female\/10-32 Male Connector in PEEK\u003c\/a\u003e\u003cspan\u003e (Cat# 20-0315) is now available for all pack sizes, providing convenient operation of the pre-packed column with a syringe at an excellent value.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eProduct Highlights\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eConvenient, easy, ready-to-use\u003c\/li\u003e\n\u003cli\u003eMechanical stable, designed for large scale purification of histidine-tagged proteins\u003c\/li\u003e\n\u003cli\u003eHigh yield (typically \u0026gt; 40 mg protein \/mL resins)\u003c\/li\u003e\n\u003cli\u003eLow leakage of nickel ions. Can be re-used for up to 8 preps*\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cspan style=\"color: #666666;\"\u003e*Note: No significant decrease in yield after 8 repetitive uses of resin when recommended buffers are used. Refer to Product Details for compatible reagents.\u003c\/span\u003e\u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eApplications\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eAutomated purifications\u003c\/li\u003e\n\u003cli\u003eRoutine His tag protein purification for structural biology and functional assays\u003c\/li\u003e\n\u003cli\u003eScaling up and preparative applications\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eTech Notes\u003c\/span\u003e\u003c\/h4\u003e\n\u003cp\u003e\u003cb\u003eOne-step purification using \u003c\/b\u003e\u003cb\u003eP\u003c\/b\u003e\u003cb\u003eroteIndex\u003c\/b\u003e\u003cb\u003e™ \u003c\/b\u003e\u003cb\u003eHiBond\u003c\/b\u003e\u003cb\u003e™ Ni-NTA \u003c\/b\u003e\u003cb\u003eAgarose 6 Fast Flow resins \u003c\/b\u003e\u003c\/p\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"35%\" style=\"border: 0px; border-color: #ffffff;\"\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Ni-NTA_6FF_2.png?v=1496201543\"\u003e\u003c\/td\u003e\n\u003ctd width=\"35%\" style=\"border: 0px; border-color: #ffffff;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cstrong\u003eFigure 1.\u003c\/strong\u003e A 15 kDa protein was expressed in E.coli and purified on an automated system using \u003cb\u003eP\u003c\/b\u003e\u003cb\u003eroteIndex\u003c\/b\u003e\u003cb\u003e™ \u003c\/b\u003e\u003cb\u003eHiBond\u003c\/b\u003e\u003cb\u003e™ Ni-NTA \u003c\/b\u003e\u003cb\u003eAgarose 6 Fast Flow resin \u003c\/b\u003eunder native conditions. 50 mL E.coli lysate in 50 mM NaH\u003csub\u003e2\u003c\/sub\u003ePO\u003csub\u003e4\u003c\/sub\u003e, 0.3 M NaCl, 10 mM imidazole was loaded on the column packed with 0.5 mL resins at the speed of 1 mL\/min. The column was then washed with 100 mL 50 mM NaH\u003csub\u003e2\u003c\/sub\u003ePO\u003csub\u003e4\u003c\/sub\u003e, 0.3 M NaCl, 20 mM imidazole at the speed of 1~2 mL\/min. The target protein was eluted with 50 mM NaH\u003csub\u003e2\u003c\/sub\u003ePO\u003csub\u003e4\u003c\/sub\u003e, 0.3 M NaCl, 250 mM imidazole. Proteins were visualized by coomassie blue staining after separation on SDS-PAGE. \u003cb\u003eL\u003c\/b\u003e: raw lysate; \u003cb\u003eF\u003c\/b\u003e: unbound flow-through; \u003cb\u003eE1\u003c\/b\u003e and \u003cb\u003eE2\u003c\/b\u003e: eluate fractions.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"37%\" style=\"border: 0px; border-color: #ffffff;\"\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Ni-NTA_6FF_comparison_with_Ni_sepharose_4.png?v=1497317795\"\u003e\u003c\/td\u003e\n\u003ctd width=\"37%\" style=\"border: 0px; border-color: #ffffff;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cstrong\u003eFigure 2.\u003c\/strong\u003e A 25 kDa protein was expressed in E.coli and purified on an automated system using a one-step automated protocol with \u003cb\u003eP\u003c\/b\u003e\u003cb\u003eroteIndex\u003c\/b\u003e\u003cb\u003e™ \u003c\/b\u003e\u003cb\u003eHiBond\u003c\/b\u003e\u003cb\u003e™ Ni-NTA \u003c\/b\u003e\u003cb\u003eAgarose 6 Fast Flow resin \u003c\/b\u003eunder native conditions. 50 mL E.coli lysate in 50 mM NaH\u003csub\u003e2\u003c\/sub\u003ePO\u003csub\u003e4\u003c\/sub\u003e, 0.3 M NaCl, 10 mM imidazole was loaded on the column packed with 0.5 mL resins at the speed of 1 mL\/min. The column was then washed with 100 mL 50 mM NaH\u003csub\u003e2\u003c\/sub\u003ePO\u003csub\u003e4\u003c\/sub\u003e, 0.3 M NaCl, 20 mM imidazole at the speed of 1~2 mL\/min. The target protein was eluted with 50 mM NaH\u003csub\u003e2\u003c\/sub\u003ePO\u003csub\u003e4\u003c\/sub\u003e, 0.3 M NaCl, 250 mM imidazole. A fraction of eluate was analyzed on a SDS-PAGE. Shown were coomassie blue stains of the target proteins purified from columns of Marvelgent’s ProteIndex Ni-NTA 6FF (\u003cstrong\u003eM\u003c\/strong\u003e) and Ni-Sepharose (\u003cstrong\u003eG\u003c\/strong\u003e) resins.\u003c\/p\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"200\"\u003e\u003cstrong\u003eLigand\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eNickel\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBinding capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;40 mg 6xHis-tagged protein\/mL medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMatrix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e6% highly cross-linked agarose\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eParticle size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e45 μm - 165 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eColumn volume\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1 mL or 5 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaximum pressure\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.3 MPa (3 bar)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eChemical compatibility\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003ctable width=\"93%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"37%\"\u003eReducing agents\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e5 mM DTE\u003cbr\u003e0.5-1 mM DTT\u003cbr\u003e20 mM β-mercaptoethanol\u003cbr\u003e5 mM TCEP\u003cbr\u003e10 mM reduced glutathione (GSH)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"37%\"\u003eDenaturing agents\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e8 M urea, 6 M Gua-HCl \u003cbr\u003e(Tested for one week at 40°C)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"37%\"\u003eDetergents\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e2% Triton™ X-100 (nonionic)\u003cbr\u003e2% Tween™ 20 (nonionic)\u003cbr\u003e2% NP-40 (nonionic)\u003cbr\u003e2% cholate (anionic)\u003cbr\u003e1% CHAPS (zwitterionic)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"37%\"\u003eOther additives\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e500 mM imidazole\u003cbr\u003e20% ethanol\u003cbr\u003e50% glycerol\u003cbr\u003e100 mM Na\u003csub\u003e2\u003c\/sub\u003eSO4\u003cbr\u003e1.5 M NaCl\u003cbr\u003e1 mM EDTA\u003cbr\u003e60 mM citrate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"37%\"\u003eCommonly used buffer\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e50 mM sodium phosphate, pH 7.4\u003cbr\u003e100 mM Tris-HCl, pH 7.4\u003cbr\u003e100 mM Tris-acetate, pH 7.4\u003cbr\u003e100 mM HEPES, pH 7.4\u003cbr\u003e100 mM MOPS, pH 7.4\u003cbr\u003e100 mM sodium acetate, pH 4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eColumn hardware pressure limit\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003e5 bar (0.5 MPa)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage buffer\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1x PBS containing 20% ethanol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProduct is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e   \u003cimg style=\"float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Keep-refrigerated_95fa1867-1f0d-47e6-823a-8a0008076e21_small.png?12332456762384261517\" alt=\"Keep refrigerated\"\u003e   \u003cimg style=\"float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Do-not-freeze_2_small.png?12332456762384261517\" alt=\"Do not freeze\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"5x 1 mL Columns","offer_id":19098127684,"sku":"11-0230-5x1ML","price":172.0,"currency_code":"USD","in_stock":true},{"title":"5x 1 mL Columns with 1 Connector","offer_id":41405616259115,"sku":"11-0230-5x1MLB","price":200.0,"currency_code":"USD","in_stock":true},{"title":"1x 5 mL Column","offer_id":19098127748,"sku":"11-0230-1x5ML","price":149.0,"currency_code":"USD","in_stock":true},{"title":"1x 5 mL Column with 1 Connector","offer_id":41405616291883,"sku":"11-0230-1x5MLB","price":177.0,"currency_code":"USD","in_stock":true},{"title":"5x 5mL Columns","offer_id":19098127812,"sku":"11-0230-5x5ML","price":539.0,"currency_code":"USD","in_stock":true},{"title":"5x 5 mL Columns with 1 Connector","offer_id":41405616324651,"sku":"11-0230-5x5MLB","price":566.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/Ni-NTA_6FF_prepacked_columns.png?v=1496933949"},{"product_id":"proteindex-co-nta-agarose-6-ff","title":"PROTEINDEX™ PureBind™ Co-NTA Agarose 6 Fast Flow","description":"\u003cp\u003e\u003cstrong\u003ePROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e\u003c\/strong\u003e™\u003cstrong\u003e\u003cstrong\u003e\u003c\/strong\u003e Co-NTA Agarose 6FF\u003c\/strong\u003e is a  cobalt-based immobilized metal ion affinity chromatography (IMAC) medium. It offers greater specificity and therefore enhanced selectivity for polyhistidine-tagged proteins under both native and denaturing conditions, compared to Nickel-charged media. Co-NTA Agarose 6 Fast Flow resins can be used for small-scale and preparative large-scale purification of histidine-tagged recombinant proteins from prokaryotic and eukaryotic expression systems.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eProduct Highlights\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cstrong\u003eMechanical stable, suited for preparative and large scale purification of histidine-tagged proteins\u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cstrong\u003eHigher specificity and selectivity for His-tagged proteins, compared with Nickel-based IMAC. Can be used to purify challenging proteins that are expressed in low levels or when the use of nickel is not desired.\u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cstrong\u003eSuitable for purifying proteins from prokaryotic and eukaryotic expression systems\u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cstrong\u003eCompatible with applications under native and denaturing conditions \u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cstrong\u003eLow cobalt ion leakage.\u003c\/strong\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"200\"\u003e\u003cstrong\u003eLigand\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eCobalt ion\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBinding capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;20 mg 6XHis-tagged protein\/mL medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMatrix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eHighly cross-linked 6% agarose resins supplied as 50% slurry\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eParticle size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e45 μm - 165 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaximum pressure\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.3 MPa (3 bar)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eChemical compatibility\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003ctable width=\"93%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"37%\" valign=\"top\"\u003eReducing agents\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e10 mM β-mercaptoethanol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"37%\" valign=\"top\"\u003eDenaturing agents\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e8 M urea, 6 M Gua-HCl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"37%\" valign=\"top\"\u003eDetergents\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e\u0026lt;1% Triton™ X-100 (nonionic)\u003cbr\u003e1% NP-40 (nonionic)\u003cbr\u003e1% CHAPS (zwitterionic)\u003cbr\u003e1% SDS\u003cbr\u003e1% sarcosyl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"37%\" valign=\"top\"\u003eOther additives\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e≤500 mM imidazole at pH7.0 ~ 8.0 for elution\u003cbr\u003e30% ethanol\u003cbr\u003e20% glycerol\u003cbr\u003e500 mM KCl\u003cbr\u003e1.0 M NaCl\u003cbr\u003e20 mM MES\u003cbr\u003e50 mM Tris\u003cbr\u003e50 mM HEPES\u003cbr\u003e60 mM MOPS\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage buffer\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1X PBS containing 20% ethanol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProduct is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e   \u003cimg alt=\"Keep refrigerated\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Keep-refrigerated_95fa1867-1f0d-47e6-823a-8a0008076e21_small.png?12332456762384261517\" style=\"float: none;\"\u003e   \u003cimg alt=\"Do not freeze\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Do-not-freeze_2_small.png?12332456762384261517\" style=\"float: none;\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eDocuments\u003c\/h5\u003e\n\u003ch4\u003eProduct Information Sheet\u003c\/h4\u003e\n\u003cp\u003e🗎    \u003ca title=\"Man_11-0231_Co-NTA_Agarose_6_Fast_Flow_v1602.pdf\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Man_11-0231_Co-NTA_Agarose_6_Fast_Flow_v1602.pdf?3603577300140654506\" target=\"_blank\"\u003eCobalt NTA Agarose 6 Fast Flow\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"10 mL","offer_id":19152276164,"sku":"11-0231-010","price":126.0,"currency_code":"USD","in_stock":true},{"title":"50 mL","offer_id":19152276228,"sku":"11-0231-050","price":495.0,"currency_code":"USD","in_stock":true},{"title":"100 mL","offer_id":19152276356,"sku":"11-0231-100","price":874.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/11-0231_Co-NTA_6FF_842a62a0-6b68-40ab-89ca-5988b601f578.png?v=1483590410"},{"product_id":"proteindex-dextrin-agarose-6-fast-flow","title":"PROTEINDEX™ Dextrin Agarose 6 Fast Flow","description":"\u003cp\u003e\u003cstrong\u003ePROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e\u003c\/strong\u003e™ \u003cstrong\u003eDextrin Agarose 6FF\u003c\/strong\u003e is a chromatography medium for purifying proteins fused to maltose binding protein (MBP-tagged protein). Recombinant proteins with the MBP-tag often have increased expression levels and higher solubility. Proper folding of the attached protein has also been shown to be promoted by the MBP tag. Since MBP increase the solubility, the tag is extremely useful for recombinant proteins that are prone to be accumulated in an insoluble form (inclusion bodies).\u003c\/p\u003e\n\u003cp\u003e\u003cspan class=\"st\" data-hveid=\"99\" data-ved=\"0ahUKEwifgtvOqo3bAhVozlkKHfsAAt8Q4EUIYzAG\"\u003eDextrin is the polymeric product resulted by enzymatic hydrolysis of amylose with beta amylase. Compared to Amylose that is susceptible to amylase degradation, Dextrin is very stable. When used as the ligand substrate immobilized on the resin for purification of the MBP tag, Dextrin offers consistently strong affinity absorption even after repeated uses. \u003cbr\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan class=\"st\" data-hveid=\"99\" data-ved=\"0ahUKEwifgtvOqo3bAhVozlkKHfsAAt8Q4EUIYzAG\"\u003eAffinity purification using Dextrin Agarose 6 Fast Flow take place under physiological conditions and mild elution is performed using maltose which preserve target protein activity. These mild conditions may even allow purification of intact protein complexes.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eThe base matrix of Dextrin Agarose 6 Fast Flow is a robust, highly cross- linked 6% agarose. The crosslinking of the base matrix has been optimized to give high physical and chemical stability, high specificity of the binding.\u003c\/p\u003e\n\u003cp\u003eOur proprietary ProteIndex™ coupling technology ensures stable immobilization of active ligand and minimal ligand leakage in a wide range of purification buffer conditions. The resins can be reused multiple times to purify the same target protein.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eProduct Highlights\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eHigh selectivity for high purity\u003c\/li\u003e\n\u003cli\u003e\u0026gt; 10 mg MBP tagged protein (80 kDa)\/mL medium\u003c\/li\u003e\n\u003cli\u003eStable coupling of ligand, low leakage; can be re-used to purify the same protein\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003e Technical Notes\u003c\/span\u003e\u003c\/h4\u003e\n\u003cp\u003e\u003cstrong\u003e \u003c\/strong\u003e \u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Dextran_6FF_3.png?v=1561146279\" alt=\"\" style=\"display: block; margin-left: auto; margin-right: auto;\" width=\"324\" height=\"250\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFigure 1. Purification performance of Dextrin 6FF resins.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e50 mL of \u003ci\u003eE.coli \u003c\/i\u003elysate containing a MBP-tagged fusion protein (~ 80 kDa) was loaded on 0.5 mL packed Dextrin 6FF resins at 0.5 mL\/mL. The column was then washed with 20 mL of Binding Buffer (20 mM Tris-HCl, 200 mM NaCl, 1 mM EDTA, pH7.4). The target MBP-tagged protein was eluted with 9.5 mL of elution buffer (20 mM Tris-HCl, 200 mM NaCl, 1 mM EDTA, 10 mM maltose, pH7.4). Fractions of lysate, flow-through, and eluate were analysed by SDS-PAGE. Lanes: (1) Raw cell lysate;  (2) Flow-through; (3) Eluate.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"200\"\u003e\u003cstrong\u003eLigand\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eDextrin\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBinding capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;10 mg MBP tagged protein (80 kDa)\/mL medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMatrix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e6% highly cross-linked agarose supplied as 50% slurry\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eParticle size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e45 μm - 165 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaximum pressure\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.3 MPa (3 bar, 43 psi)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eRecommended flow rate\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003e300 - 500 cm\/hour\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003epH stability\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003e3-12\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage buffer\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e20% ethanol as preservative\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProduct is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e   \u003cimg alt=\"Keep refrigerated\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Keep-refrigerated_95fa1867-1f0d-47e6-823a-8a0008076e21_small.png?12332456762384261517\" style=\"float: none;\"\u003e   \u003cimg alt=\"Do not freeze\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Do-not-freeze_2_small.png?12332456762384261517\" style=\"float: none;\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"10 mL","offer_id":12143023816747,"sku":"11-0218-010","price":147.0,"currency_code":"USD","in_stock":true},{"title":"25 mL","offer_id":12143023882283,"sku":"11-0218-025","price":317.0,"currency_code":"USD","in_stock":true},{"title":"100 mL","offer_id":12143023915051,"sku":"11-0218-100","price":1062.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/Dextran_6FF.png?v=1559231595"},{"product_id":"proteindex-anti-dykddddk-agarose-4-fast-flow","title":"PROTEINDEX™ Anti-DYKDDDDK Agarose 4 Fast Flow","description":"\u003cp\u003e\u003cstrong\u003ePROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e\u003c\/strong\u003e™ Anti-DYKDDDDK Agarose 4 Fast Flow is an affinity chromatography medium for the purification of proteins that contain the peptide sequence DYKDDDDK (N-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-C). The ligand covalently immobilized on the matrix support is a recombinant mouse monoclonal IgG1 species that binds the same epitope as Sigma's Anti-FLAG® M2 Antibody, and it is highly specific for the peptide DYKDDDDK in a calcium-independent manner. Commonly known as the FLAG® epitope, the amino acid sequence DYKDDDDK has been used as an affinity tag to fuse at the N-terminal, C-terminal, or an internal location of a recombinant protein.\u003c\/p\u003e\n\u003cp\u003eFor protein purification, the ProteIndex™ Anti-DYKDDDDK Agarose 4FF resin is added to the sample. Following capture of the DYKDDDDK-tagged protein, the unbound and non-specifically bound proteins can be washed away. The bound target protein can then be eluted either with the competitive peptide under mild conditions or with the acidic glycine-HCl buffer.  \u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eBecause the binding between the epitope and the antibody can occur under the native conditions, any proteins or other macromolecules that are able to interact with the tagged fusion protein will also be captured during the binding step. Therefore, the anti-DYKDDDDK 4FF affinity resin can be used in co-immunoprecipitation to analyze the protein complex that the target fusion protein is associated with.\u003c\/p\u003e\n\u003cp\u003eThe base matrix of ProteIndex™ Anti-DYKDDDDK Agarose 4FF is highly cross-linked 4% agarose, a robust support offering low background and high physical stability for chromatography procedures at various scales. The affinity resin is recommended for use in gravity\/batch applications, spin purification columns, or cartridges for FPLC instruments.  \u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eProduct Highlights\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eHigh selectivity for high purity (\u0026gt;90%)\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003e\u0026gt; 3 mg DYKDDDDK-tagged protein (50 kDa)\/mL medium\u003c\/li\u003e\n\u003cli\u003eStable coupling of ligand, low leakage; can be re-used to purify the same protein (3~5 repeated uses when acid elution is employed)\u003c\/li\u003e\n\u003cli\u003eVersatile - ready for gravity\/batch, spin columns, and FPLC applications\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003e Technical Notes\u003c\/span\u003e\u003c\/h4\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e \u003cstrong\u003eA.\u003c\/strong\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003eB.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cdiv style=\"text-align: left;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Anti-DYKDDDDK_4FF_peptide_elution_730c0dc2-7077-4c4d-b82a-83fef6e52ed4_480x480.png?v=1622772785\" alt=\"\"\u003e\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cdiv style=\"text-align: left;\"\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Anti-DYKDDDDK_4FF_glycin_elution_e2c690ae-2c1d-4d94-b884-abdfb05a1c35_480x480.png?v=1622772895\"\u003e\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003cstrong\u003eFigure 1. \u003c\/strong\u003e\u003cb\u003ePurification performance of ProteIndex™ Anti-\u003c\/b\u003e\u003cb\u003eDYKDDDDK \u003c\/b\u003e\u003cb\u003e4\u003c\/b\u003e\u003cb\u003eFF affinity resins.\u003c\/b\u003e\u003c\/p\u003e\n\u003cp\u003eWhole cell lysate containing a DYKDDDDK-tagged fusion protein (~ 50 kDa) was loaded on the column packed with the anti-DYKDDDDK 4FF affinity resin. The column was washed with Binding\/Wash Buffer (50 mM Tris，0.15 M NaCl，pH7.4). The target DYKDDDDK-tagged protein was partially eluted with 2 column volumes of 3x DYKDDDDK peptide (0.1 mg\/mL, A) or the glycine-HCl buffer (B).\u003c\/p\u003e\n\u003cp\u003eShown in the SDS-PAGE gel images, from left to right fractions are: lysate (CL), flow-through (FT), eluate (E), and the supernatant of the boiled resin in the SDS-sample buffer (B).\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eThis product can be packed into column for FPLC on automated systems. If you are interested in the pre-packed cartridge formats, talk to \u003ca href=\"mailto:cust.support@marvelgent.com\"\u003eour customer support\u003c\/a\u003e for further assistance.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"200\"\u003e\u003cstrong\u003eLigand\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMouse monoclonal anti-DYKDDDDK IgG1, calcium-independent\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBinding capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e≥ 3 mg DYKDDDDK-tagged protein (~ 50 kDa) per mL medium\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003ePurity \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e≥ 90%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMatrix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e4% highly cross-linked agarose supplied as 50% slurry\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eParticle size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e45 μm - 165 μm with the average particle size being 90 μm\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaximum pressure\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.3 MPa (3 bar) \u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eRecommended flow rate\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cspan data-mce-fragment=\"1\" class=\"ng-binding ng-scope\" ng-bind-html=\"main.bindDemiliter($last, value + ' ' + feature.unitSymbol, '; ')\" ng-repeat=\"value in feature.values\"\u003e30 - 250\u003c\/span\u003e cm\/hour\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003epH stability\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003e3-12\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage buffer\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1x PBS with 0.02% NaN\u003csub data-mce-fragment=\"1\"\u003e3\u003c\/sub\u003e\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProduct is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e   \u003cimg style=\"float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Keep-refrigerated_95fa1867-1f0d-47e6-823a-8a0008076e21_small.png?12332456762384261517\" alt=\"Keep refrigerated\"\u003e   \u003cimg style=\"float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Do-not-freeze_2_small.png?12332456762384261517\" alt=\"Do not freeze\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"2 mL","offer_id":39336067137579,"sku":"18-0270-002","price":451.0,"currency_code":"USD","in_stock":true},{"title":"10 mL","offer_id":39336067170347,"sku":"18-0270-010","price":1668.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/18-0270Anti-DYKDDDDK4FF_3.png?v=1623432010"}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/collections\/Marvelgent_website_icon_-_beads2.png?v=1781630186","url":"https:\/\/marvelgent.com\/collections\/marvelgent-chromatography-resins-technologies\/ligand-functional-group_anti-dykddddk-mab.oembed","provider":"Marvelgent Biosciences","version":"1.0","type":"link"}