{"title":"Popular Products","description":"","products":[{"product_id":"proteindex-ni-penta-agarose-chemical-tolerant","title":"PROTEINDEX™ Ni-Penta™ Agarose, Chemical Stable","description":"\u003cp\u003e\u003cstrong\u003ePROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e\u003c\/strong\u003e™ \u003cstrong\u003eChemical-Tolerant Ni-Penta™ Agarose\u003c\/strong\u003e is a new immobilized metal ion affinity chromatography (IMAC) medium precharged with nickel ions, designed mainly for purification of low abundant proteins or challenging proteins in conditions where EDTA, DTT, β-ME, and other stabilizing agents are necessary for maintaining protein stability and integrity.\u003c\/p\u003e\n\u003cp\u003eThe nickel ion binds very strongly to the matrix support in complex with a proprietary pentadentate chelating ligand, which immobilizes the nickel ion through 5 sites to form a homogeneous,  chemical-stable structure. This characteristic nickel ion bonding on the Ni-Penta™ resin provides excellent resistance to EDTA, NaOH, β-ME, and other reagents that are restricted in the purification protocol using conventional NTA- and IDA- functionalized resins.\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"http:\/\/marvelgent.com\/pages\/high-selectivity-ni-penta-agarose-resins-for-secreted-proteins\" title=\"Ni-Penta resins for high selective capture of polyHis-tagged proteins\"\u003eRead more on Ni-Penta properties contributing to high selective purification of His-tagged proteins ...\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eProduct Highlights\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eEDTA-resistant\u003c\/li\u003e\n\u003cli\u003eHigh selectivity for high purity\u003c\/li\u003e\n\u003cli\u003eRetained high 6xHis affinity in various buffers\u003c\/li\u003e\n\u003cli\u003eWell suited for direct capture of secreted polyHis-tagged proteins from eukaryotic cell culture supernatant\u003c\/li\u003e\n\u003cli\u003eRecharge-free with minimal nickel ion leakage\u003c\/li\u003e\n\u003cli\u003eRequires less imidazole\u003c\/li\u003e\n\u003cli\u003eNaOH-tolerant\u003c\/li\u003e\n\u003cli\u003eVersatile, ready-to-use with convenient formats\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eApplications:\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cstrong\u003ePurification of secreted His-tagged proteins directly from eukaryotic cell culture supernatants\u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003eOwing to its proprietary ligand for nickel ion immobilization, Ni-Penta™ resin tolerates EDTA very well, and its affinity for His-tag can be retained in presence of EDTA up to 10 mM. To purify secreted proteins that are produced from eukaryotic cell cultures (such as insect High Five™ cells* and Sf9 cells, CHO cells, 293 cells etc.), a step of removing incompatible agents to Ni-NTA resins by dialysis is frequently required prior to loading the material on the column. This is tedious, time-consuming, and labor intensive. By using Ni-Penta resins, sample pretreament can be skipped, and the culture medium can be loaded directly to the column for purification.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cstrong\u003ePurification of His-tagged proteins that require presence of EDTA, DTT, β-ME, and other agents for maximum protein stability and integrity\u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003ePROTEINDEX™ Ni-Penta™ Agarose Resin is compatible with a wide range of buffers including chelating agents (e.g. EDTA) and reducing agents (e.g. DTT), which are incompatible and therefore should be avoid in purification applications using conventional NTA or IDA resins. Ni-Penta™ resin helps to simplify your sample preparation process and allows your target proteins be purified at their preferred conditions.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cstrong\u003eRepetitive uses of the column that require clean-in-place with NaOH but no nickel recharge \u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003eAnti-strip power of the Ni-Penta™ enables repetitive uses of the column without the need to recharge nickel ion, even after multiple cycles of CIP with 1 M NaOH.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cstrong\u003eRestricted imidazole amount in the elution buffer due to the requirements in downstream applications or the target protein's sensitivity to imidazole\u003cbr\u003e\u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003eImidazole is general required to minimize non-specific binding on Ni-NTA resins. Ni-Penta™ Agarose Resin is highly selective for His-tag and therefore does not demand imidazole for minimizing non-specific binding throughout purification. No imidazole is required in the binding buffer, and 40 mM to 100 mM of imidazole can efficiently elute most target proteins.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003e Technical Notes\u003c\/span\u003e\u003c\/h4\u003e\n\u003ch4\u003e 1. EDTA-stable Ni-Penta chemistry for minimal nickel ion leakage\u003c\/h4\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Ni-Penta_8_99b871bb-a5a2-4123-8891-2437bac436d6.png?v=1493146106\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eFigure 1. Strong chelate chemistry for immobilization of nickel ion. \u003c\/b\u003eNi-Penta Agarose 6 FF resins were incubated at room temperature for 2 hours in 5 volumes of a serial dilutions of EDTA from 10 mM to 400 mM. The amount of nickel ions released into the buffer was measured.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eResult:\u003c\/strong\u003e During 2 hours of incubation in 50 mM EDTA, less than 5 % of nickel ions leaked from Ni-Penta resins. In comparison, 50 mM EDTA is sufficient to strip off nickel ions from conventional Ni-NTA resins and has been used routinely to regenerate Ni-NTA resin.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e2. Uncompromised His-tag protein binding in EDTA up to 10 mM\u003c\/h4\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"color: #3d85c6;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Ni-Penta_7_ad68e7f9-16be-43e9-a619-dcfe21d2590f.png?v=1493145893\" alt=\"\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFigure 2. Retained His-tagged protein binding capacity in presence of EDTA.\u003c\/strong\u003e Ni-Penta 6FF resins were added to 20 volumes of E.coli lysate (~0.5 mg\/mL containing a His-tagged protein of 26 kDa in size), in the presence of 2 mM, 5 mM, 10 mM EDTA respectively. Protein binding was then performed at room temperature for 2 hours. After washing 5 times with 20mM sodium phosphate, 0.5 M NaCl, 5 mM imidazole, pH7.4, the target His-tagged protein was eluted with 20mM sodium phosphate, 0.5 M NaCl, 250 mM imidazole, pH7.4. Protein concentration was measured by UV absorbance at OD280.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eResult:\u003c\/strong\u003e In the presence of 10 mM EDTA, the amount of His-tag protein being purified from Ni-Penta resin was similar to those being purified in the presence of less or no EDTA.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e3. Tolerance to reducing agents\u003c\/h4\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Ni-Penta_9.png?v=1491964108\" alt=\"Ni-penta Tolerance to reducing agents\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eFigure 3. Tolerance to reducing agents. \u003c\/b\u003eNi-Penta 6FF resins were added to 20 volumes of \u003ci\u003eE.coli \u003c\/i\u003elysate (~0.5 mg\/mL containing a His-tagged protein of 26 kDa in size), in the presence of various dilutions of reducing agents, including TCEP, DTT, and β-ME respectively. Protein binding was then performed at room temperature for 2 hours. After washing 5 times with 20mM sodium phosphate, 0.5 M NaCl, 5 mM imidazole, pH7.4, the target His-tagged protein was eluted with 20mM sodium phosphate, 0.5 M NaCl, 250 mM imidazole, pH7.4. Protein concentration was measured by UV absorbance at OD280.\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eResult: \u003c\/b\u003eIn the presence of up to 5 mM TCEP, 10 mM DTT, or 50 mM β-ME, the amount of His-tag protein being purified from Ni-Penta resin was similar to those being purified in absence of the respective reducing agents.\u003c\/p\u003e\n\u003cp\u003e(A) The percentage of retained protein binding capacity is plotted against the concentration of individual reducing agents. (B) Representative SDS-PAGE analysis of the eluates.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e*\u003c\/strong\u003e High Five™ is a trade mark of Thermo Fisher Scientific.\u003c\/p\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"200\"\u003e\u003cstrong\u003eLigand\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eNickel\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBinding capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;10 mg 6XHis-tagged protein\/mL medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMatrix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e4% agarose supplied as 50% slurry\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eParticle size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e45 μm - 165 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaximum pressure\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.1 MPa (1 bar)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eRecommended flow rate\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003eGravity flow, \u0026lt;75 cm\/hour\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eChemical compatibility\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cul\u003e\n\u003cli\u003e0.01 M HCl ,0.01 M NaOH, for ≥ 1 week;\u003c\/li\u003e\n\u003cli\u003e10 mM EDTA, 1 M NaOH, 5 mM DTT, 5 mM TCEP, 20 mM β-ME, 6 M guanidine-HCl, for ≥ 24 hours\u003c\/li\u003e\n\u003cli\u003e500 mM imidazole，100 mM EDTA, for ≥ 2 hours\u003c\/li\u003e\n\u003cli\u003e30% isopropanol for ≥ 20 mins\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage buffer\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1X PBS containing 20% ethanol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProduct is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e   \u003cimg alt=\"Keep refrigerated\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Keep-refrigerated_95fa1867-1f0d-47e6-823a-8a0008076e21_small.png?12332456762384261517\" style=\"float: none;\"\u003e   \u003cimg alt=\"Do not freeze\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Do-not-freeze_2_small.png?12332456762384261517\" style=\"float: none;\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eDocuments\u003c\/h5\u003e\n\u003ch4\u003eProduct Information Sheet\u003c\/h4\u003e\n\u003cp\u003e🗎    \u003ca title=\"Man_11-0227_Chemical-Tolerant Ni-Penta Agarose_v1603.pdf\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Man_11-0227_Chemical-Tolerant_Ni-Penta_Agarose_v1603.pdf?9251540950763704712\" target=\"_blank\"\u003eNi-Penta™ Agarose\u003c\/a\u003e\u003c\/p\u003e\n\u003ch4 style=\"color: #ee8012;\"\u003e\u003cbr\u003e\u003c\/h4\u003e\n\u003cp style=\"text-align: left;\"\u003e \u003c\/p\u003e\n\u003ch5\u003eAccessories\u003c\/h5\u003e\n\u003cp\u003e\u003ca title=\"Disposable gravity flow columns\" href=\"http:\/\/marvelgent.com\/collections\/empty-gravity-flow-columns\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/Gravity_columns_730e2b1d-64df-4e61-96c2-f2f8c076c2d6_compact.png?v=1468002569\" alt=\"\"\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca title=\"Disposable gravity flow columns\" href=\"http:\/\/marvelgent.com\/collections\/empty-gravity-flow-columns\"\u003eDisposable Gravity Flow Columns\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"10 mL","offer_id":17018319684,"sku":"11-0227-010","price":146.0,"currency_code":"USD","in_stock":true},{"title":"50 mL","offer_id":17018319812,"sku":"11-0227-050","price":586.0,"currency_code":"USD","in_stock":true},{"title":"100 mL","offer_id":17018319876,"sku":"11-0227-100","price":1051.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/11-0227_Ni-Penta.png?v=1490835874"},{"product_id":"proteindex-ni-penta-agarose-6ff-chemical-tolerant","title":"PROTEINDEX™ Ni-Penta™ Agarose 6 Fast Flow, Chemical Stable","description":"\u003cp\u003e\u003cstrong\u003ePROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e\u003c\/strong\u003e™ \u003cstrong\u003eChemical-Tolerant Ni-Penta™ Agarose 6FF\u003c\/strong\u003e is a new immobilized metal ion affinity chromatography (IMAC) medium precharged with nickel ions, designed mainly for purification of low abundant proteins or challenging proteins in conditions where EDTA, DTT, β-ME, and other stabilizing agents are necessary for maintaining protein stability and integrity.\u003c\/p\u003e\n\u003cp\u003eThe nickel ion binds very strongly to the matrix support in complex with a proprietary pentadentate chelating ligand, which immobilizes the nickel ion through 5 sites to form a homogeneous,  chemical-stable structure. This characteristic nickel ion bonding on the Ni-Penta™ resin provides excellent resistance to EDTA, NaOH, β-ME, and other reagents that are restricted in the purification protocol using conventional NTA- and IDA- functionalized resins. In particular, Ni-Penta™ Agarose 6FF is well suited for direct capture of secreted polyHis-tagged proteins from the cell culture supernatant of eukaryotic cells, including CHO cells, insect High Five™ cells* and Sf9 cells.\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"http:\/\/marvelgent.com\/pages\/high-selectivity-ni-penta-agarose-resins-for-secreted-proteins\" title=\"Ni-Penta resins for high selective capture of polyHis-tagged proteins\"\u003eRead more on Ni-Penta properties contributing to high selective purification of His-tagged proteins ...\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003eHighly cross-linked 6% agarose as the base matrix support offers excellent mechanical stability for automated, scale up purification of histidine-tagged proteins.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eProduct Highlights\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eEDTA-resistant\u003c\/li\u003e\n\u003cli\u003eHigh selectivity for high purity\u003c\/li\u003e\n\u003cli\u003eRetained high 6xHis affinity in various buffers\u003c\/li\u003e\n\u003cli\u003eRecharge-free with minimal nickel ion leakage\u003c\/li\u003e\n\u003cli\u003eRequires less imidazole\u003c\/li\u003e\n\u003cli\u003eNaOH-tolerant, allowing CIP with 1 M NaOH\u003c\/li\u003e\n\u003cli\u003eVersatile, ready-to-use with convenient formats\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eApplications:\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cstrong\u003ePurification of secreted His-tagged proteins directly from eukaryotic cell culture supernatants\u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003eOwing to its proprietary ligand for nickel ion immobilization, Ni-Penta™ resin tolerates EDTA very well, and its affinity for His-tag can be retained in presence of EDTA up to 10 mM. To purify secreted proteins that are produced from eukaryotic cell cultures (such as insect cells, CHO cells, 293 cells etc.), a step of removing incompatible agents to Ni-NTA resins by dialysis is frequently required prior to loading the material on the column. This is tedious, time-consuming, and labor intensive. By using Ni-Penta resins, sample pretreament can be skipped, and the culture medium can be loaded directly to the column for purification.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cstrong\u003ePurification of His-tagged proteins that require presence of EDTA, DTT, β-ME, and other agents for maximum protein stability and integrity\u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003ePROTEINDEX™ Ni-Penta™ Agarose Resin is compatible with a wide range of buffers including chelating agents (e.g. EDTA) and reducing agents (e.g. DTT), which are incompatible and therefore should be avoid in purification applications using conventional NTA or IDA resins. Ni-Penta™ resin helps to simplify your sample preparation process and allows your target proteins be purified at their preferred conditions.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cstrong\u003eRepetitive uses of the column that require clean-in-place with NaOH but no nickel recharge \u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003eAnti-strip power of the Ni-Penta™ enables repetitive uses of the column without the need to recharge nickel ion, even after multiple cycles of CIP with 1 M NaOH.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cstrong\u003eRestricted imidazole amount in the elution buffer due to the requirements in downstream applications or the target protein's sensitivity to imidazole\u003cbr\u003e\u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003eImidazole is general required to minimize non-specific binding on Ni-NTA resins. Ni-Penta™ Agarose Resin is highly selective for His-tag and therefore does not demand imidazole for minimizing non-specific binding throughout purification. No imidazole is required in the binding buffer, and 40 mM to 100 mM of imidazole can efficiently elute most target proteins.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003e Technical Notes\u003c\/span\u003e\u003c\/h4\u003e\n\u003ch4\u003e 1. EDTA-stable Ni-Penta chemistry for minimal nickel ion leakage\u003c\/h4\u003e\n\u003cp style=\"text-align: center;\"\u003e \u003cimg alt=\"EDTA-stable chelate chemistry for immobilization of nickel ion.\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Ni-Penta_8_grande.png?v=1491963528\" style=\"float: none;\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eFigure 1. Strong chelate chemistry for immobilization of nickel ion. \u003c\/b\u003e\u003c\/p\u003e\n\u003cp\u003eNi-Penta Agarose 6 FF resins were incubated at room temperature for 2 hours in 5 volumes of a serial dilutions of EDTA from 10 mM to 400 mM. The amount of nickel ions released into the buffer was measured.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eResult:\u003c\/strong\u003e During 2 hours of incubation in 50 mM EDTA, less than 5 % of nickel ions leaked from Ni-Penta resins. In comparison, 50 mM EDTA is sufficient to strip off nickel ions from conventional Ni-NTA resins and has been used routinely to regenerate Ni-NTA resin.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e2. Uncompromised His-tag protein binding in EDTA up to 10 mM\u003c\/h4\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"color: #3d85c6;\"\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Ni-Penta_7_654f089b-e6ea-413d-9b06-abb3ead360a8_grande.png?v=1491962523\" style=\"float: none;\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFigure 2. Retained His-tagged protein binding capacity in presence of EDTA.\u003c\/strong\u003e Ni-Penta 6FF resins were added to 20 volumes of E.coli lysate (~0.5 mg\/mL containing a His-tagged protein of 26 kDa in size), in the presence of 2 mM, 5 mM, 10 mM EDTA respectively. Protein binding was then performed at room temperature for 2 hours. After washing 5 times with 20mM sodium phosphate, 0.5 M NaCl, 5 mM imidazole, pH7.4, the target His-tagged protein was eluted with 20mM sodium phosphate, 0.5 M NaCl, 250 mM imidazole, pH7.4. Protein concentration was measured by UV absorbance at OD280.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eResult:\u003c\/strong\u003e In the presence of 10 mM EDTA, the amount of His-tag protein being purified from Ni-Penta resin was similar to those being purified in the presence of less or no EDTA.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e3. Tolerance to reducing agents\u003c\/h4\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Ni-Penta_9.png?v=1491964108\" alt=\"Ni-penta Tolerance to reducing agents\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eFigure 3. Tolerance to reducing agents. \u003c\/b\u003e\u003c\/p\u003e\n\u003cp\u003eNi-Penta 6FF resins were added to 20 volumes of \u003ci\u003eE.coli \u003c\/i\u003e lysate (~0.5 mg\/mL containing a His-tagged protein of 26 kDa in size), in the presence of various dilutions of reducing agents, including TCEP, DTT, and β-ME respectively. Protein binding was then performed at room temperature for 2 hours. After washing 5 times with 20mM sodium phosphate, 0.5 M NaCl, 5 mM imidazole, pH7.4, the target His-tagged protein was eluted with 20mM sodium phosphate, 0.5 M NaCl, 250 mM imidazole, pH7.4. Protein concentration was measured by UV absorbance at OD280.\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eResult: \u003c\/b\u003eIn the presence of up to 5 mM TCEP, 10 mM DTT, or 50 mM β-ME, the amount of His-tag protein being purified from Ni-Penta resin was similar to those being purified in absence of the respective reducing agents.\u003c\/p\u003e\n\u003cp\u003e(A) The percentage of retained protein binding capacity is plotted against the concentration of individual reducing agents. (B) Representative SDS-PAGE analysis of the eluates.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e*\u003c\/strong\u003e High Five™ is a trade mark of Thermo Fisher Scientific.\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"200\"\u003e\u003cstrong\u003eLigand\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eNickel\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBinding capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;10 mg 6XHis-tagged protein\/mL medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMetal ion capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u0026gt; 50 µmol Ni\u003csup\u003e2+ \u003c\/sup\u003e\/ ml medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMatrix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eHighly cross-linked 6% agarose resin supplied as 50% slurry\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eParticle size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e45 μm - 165 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaximum pressure\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.3 MPa (3 bar)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eChemical compatibility\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cul\u003e\n\u003cli\u003e0.01 M HCl ,0.01 M NaOH, for ≥ 1 week;\u003c\/li\u003e\n\u003cli\u003e10 mM EDTA, 1 M NaOH, 5 mM DTT, 5 mM TCEP, 20 mM β-ME, 6 M guanidine-HCl, for ≥ 24 hours\u003c\/li\u003e\n\u003cli\u003e500 mM imidazole，100 mM EDTA, for ≥ 2 hours\u003c\/li\u003e\n\u003cli\u003e30% isopropanol for ≥ 20 mins\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage buffer\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1X PBS containing 20% ethanol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProduct is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e   \u003cimg alt=\"Keep refrigerated\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Keep-refrigerated_95fa1867-1f0d-47e6-823a-8a0008076e21_small.png?12332456762384261517\" style=\"float: none;\"\u003e   \u003cimg alt=\"Do not freeze\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Do-not-freeze_2_small.png?12332456762384261517\" style=\"float: none;\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eDocuments\u003c\/h5\u003e\n\u003ch4\u003eProduct Information Sheet\u003c\/h4\u003e\n\u003cp\u003e🗎    \u003ca title=\"Man_11-0228_Chemical-Tolerant Ni-Penta Agarose6FF_v1603.pdf\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Man_11-0228_Chemical-Tolerant_Ni-Penta_Agarose_6_Fast_Flow_v1603.pdf?4531069748310472707\" target=\"_blank\"\u003eNi-Penta™ Agarose 6FF\u003c\/a\u003e\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"10 mL","offer_id":17033312580,"sku":"11-0228-010","price":146.0,"currency_code":"USD","in_stock":true},{"title":"50 mL","offer_id":17033312644,"sku":"11-0228-050","price":586.0,"currency_code":"USD","in_stock":true},{"title":"100 mL","offer_id":17033312708,"sku":"11-0228-100","price":1051.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/Ni-Penta.png?v=1483497352"},{"product_id":"proteindex-ni-penta-agarose-6-fast-flow-chemical-stable-br-prepacked-cartridge","title":"PROTEINDEX™ Ni-Penta™ Agarose 6 Fast Flow, Chemical Stable, Prepacked Cartridge","description":"\u003cp\u003e\u003cstrong\u003eROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e\u003c\/strong\u003e™ \u003cstrong\u003eChemical-Tolerant Ni-Penta™ Agarose 6FF\u003c\/strong\u003e is a new immobilized metal ion affinity chromatography (IMAC) medium precharged with nickel ions, designed mainly for purification of low abundant proteins or challenging proteins in conditions where EDTA, DTT, β-ME, and other stabilizing agents are necessary for maintaining protein stability and integrity.\u003c\/p\u003e\n\u003cp\u003eThe nickel ion binds very strongly to the matrix support in complex with a proprietary pentadentate chelating ligand, which immobilizes the nickel ion through 5 sites to form a homogeneous,  chemical-stable structure. This characteristic nickel ion bonding on the Ni-Penta™ resin provides excellent resistance to EDTA, NaOH, β-ME, and other reagents that are restricted in the purification protocol using conventional NTA- and IDA- functionalized resins. In particular, Ni-Penta™ Agarose 6FF is well suited for direct capture of secreted polyHis-tagged proteins from the cell culture supernatant of eukaryotic cells, including CHO cells, insect High Five™ cells* and Sf9 cells.\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"http:\/\/marvelgent.com\/pages\/high-selectivity-ni-penta-agarose-resins-for-secreted-proteins\" title=\"Ni-Penta resins for high selective capture of polyHis-tagged proteins\"\u003eRead more on Ni-Penta properties contributing to high selective purification of His-tagged proteins ...\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003eNi-Penta™ Agarose 6 FF Prepacked Cartridge are packed with 1 ml and 5 ml of Ni-Penta Agarose 6 Fast Flow. The columns have standard interface that can be adapted to most commonly used chromatography systems, such as ÄKTA.\u003c\/p\u003e\n\u003cp\u003ePrepacked 16\/10 columns and 7\/10 columns offer a bed height of 10 cm that is convenient for process scale-up and development.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eValue bundle with a \u003ca rel=\"noopener\" title=\"Connector Luer Female \/ 10-32 Male in PEEK\" href=\"https:\/\/marvelgent.com\/products\/connector-1-16-luer-female-10-32-male-peek\" target=\"_blank\"\u003eLuer Female\/10-32 Male Connector in PEEK\u003c\/a\u003e (Cat# 20-0315) is now available for all pack sizes, providing convenient operation of the pre-packed column with a syringe at an excellent value.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eProduct Highlights\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eEDTA-resistant\u003c\/li\u003e\n\u003cli\u003eHigh selectivity for high purity\u003c\/li\u003e\n\u003cli\u003eRetained high 6xHis affinity in various buffers\u003c\/li\u003e\n\u003cli\u003eRecharge-free with minimal nickel ion leakage\u003c\/li\u003e\n\u003cli\u003eRequires less imidazole\u003c\/li\u003e\n\u003cli\u003eNaOH-tolerant, allowing CIP with 1 M NaOH\u003c\/li\u003e\n\u003cli\u003eReady-to-use, convenient formats: 1 mL and 5 mL\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eApplications:\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cstrong\u003ePurification of secreted His-tagged proteins directly from eukaryotic cell culture supernatants\u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003eOwing to its proprietary ligand for nickel ion immobilization, Ni-Penta™ resin tolerates EDTA very well, and its affinity for His-tag can be retained in presence of EDTA up to 10 mM. To purify secreted proteins that are produced from eukaryotic cell cultures (such as insect cells, CHO cells, 293 cells etc.), a step of removing incompatible agents to Ni-NTA resins by dialysis is frequently required prior to loading the material on the column. This is tedious, time-consuming, and labor intensive. By using Ni-Penta resins, sample pretreament can be skipped, and the culture medium can be loaded directly to the column for purification.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cstrong\u003ePurification of His-tagged proteins that require presence of EDTA, DTT, β-ME, and other agents for maximum protein stability and integrity\u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003ePROTEINDEX™ Ni-Penta™ Agarose Resin is compatible with a wide range of buffers including chelating agents (e.g. EDTA) and reducing agents (e.g. DTT), which are incompatible and therefore should be avoid in purification applications using conventional NTA or IDA resins. Ni-Penta™ resin helps to simplify your sample preparation process and allows your target proteins be purified at their preferred conditions.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cstrong\u003eRepetitive uses of the column that require clean-in-place with NaOH but no nickel recharge \u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003eAnti-strip power of the Ni-Penta™ enables repetitive uses of the column without the need to recharge nickel ion, even after multiple cycles of CIP with 1 M NaOH.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cstrong\u003eRestricted imidazole amount in the elution buffer due to the requirements in downstream applications or the target protein's sensitivity to imidazole\u003cbr\u003e\u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003eImidazole is general required to minimize non-specific binding on Ni-NTA resins. Ni-Penta™ Agarose Resin is highly selective for His-tag and therefore does not demand imidazole for minimizing non-specific binding throughout purification. No imidazole is required in the binding buffer, and 40 mM to 100 mM of imidazole can efficiently elute most target proteins.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\n\u003cspan style=\"color: #3d85c6;\"\u003e Technical Notes\u003c\/span\u003e \u003c\/h4\u003e\n\u003ch4\u003e1. EDTA-stable Ni-Penta chemistry for minimal nickel ion leakage\u003c\/h4\u003e\n\u003cp style=\"text-align: center;\"\u003e \u003cimg alt=\"EDTA-stable chelate chemistry for immobilization of nickel ion.\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Ni-Penta_8_grande.png?v=1491963528\" style=\"float: none;\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eFigure 1. Strong chelate chemistry for immobilization of nickel ion. \u003c\/b\u003eNi-Penta Agarose 6 FF resins were incubated at room temperature for 2 hours in 5 volumes of a serial dilutions of EDTA from 10 mM to 400 mM. The amount of nickel ions released into the buffer was measured.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eResult:\u003c\/strong\u003e During 2 hours of incubation in 50 mM EDTA, less than 5 % of nickel ions leaked from Ni-Penta resins. In comparison, 50 mM EDTA is sufficient to strip off nickel ions from conventional Ni-NTA resins and has been used routinely to regenerate Ni-NTA resin.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e2. Uncompromised His-tag protein binding in EDTA up to 10 mM\u003c\/h4\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"color: #3d85c6;\"\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Ni-Penta_7_654f089b-e6ea-413d-9b06-abb3ead360a8_grande.png?v=1491962523\" style=\"float: none;\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFigure 2. Retained His-tagged protein binding capacity in presence of EDTA.\u003c\/strong\u003e Ni-Penta 6FF resins were added to 20 volumes of E.coli lysate (~0.5 mg\/mL containing a His-tagged protein of 26 kDa in size), in the presence of 2 mM, 5 mM, 10 mM EDTA respectively. Protein binding was then performed at room temperature for 2 hours. After washing 5 times with 20mM sodium phosphate, 0.5 M NaCl, 5 mM imidazole, pH7.4, the target His-tagged protein was eluted with 20mM sodium phosphate, 0.5 M NaCl, 250 mM imidazole, pH7.4. Protein concentration was measured by UV absorbance at OD280.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eResult:\u003c\/strong\u003e In the presence of 10 mM EDTA, the amount of His-tag protein being purified from Ni-Penta resin was similar to those being purified in the presence of less or no EDTA.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e3. Tolerance to reducing agents\u003c\/h4\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Ni-Penta_9.png?v=1491964108\" alt=\"Ni-penta Tolerance to reducing agents\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eFigure 3. Tolerance to reducing agents. \u003c\/b\u003eNi-Penta 6FF resins were added to 20 volumes of \u003ci\u003eE.coli \u003c\/i\u003elysate (~0.5 mg\/mL containing a His-tagged protein of 26 kDa in size), in the presence of various dilutions of reducing agents, including TCEP, DTT, and β-ME respectively. Protein binding was then performed at room temperature for 2 hours. After washing 5 times with 20mM sodium phosphate, 0.5 M NaCl, 5 mM imidazole, pH7.4, the target His-tagged protein was eluted with 20mM sodium phosphate, 0.5 M NaCl, 250 mM imidazole, pH7.4. Protein concentration was measured by UV absorbance at OD280.\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eResult: \u003c\/b\u003eIn the presence of up to 5 mM TCEP, 10 mM DTT, or 50 mM β-ME, the amount of His-tag protein being purified from Ni-Penta resin was similar to those being purified in absence of the respective reducing agents.\u003c\/p\u003e\n\u003cp\u003e(A) The percentage of retained protein binding capacity is plotted against the concentration of individual reducing agents. (B) Representative SDS-PAGE analysis of the eluates.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e*\u003c\/strong\u003e High Five™ is a trade mark of Thermo Fisher Scientific.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"200\"\u003e\u003cstrong\u003eLigand\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eNickel\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBinding capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;10 mg 6XHis-tagged protein\/mL medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMatrix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eHighly cross-linked 6% agarose resin\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eParticle size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e45 μm - 165 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaximum pressure\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.3 MPa (3 bar)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eChemical compatibility\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cul\u003e\n\u003cli\u003e0.01 M HCl ,0.01 M NaOH, for ≥ 1 week;\u003c\/li\u003e\n\u003cli\u003e10 mM EDTA, 1 M NaOH, 5 mM DTT, 5 mM TCEP, 20 mM β-ME, 6 M guanidine-HCl, for ≥ 24 hours\u003c\/li\u003e\n\u003cli\u003e500 mM imidazole，100 mM EDTA, for ≥ 2 hours\u003c\/li\u003e\n\u003cli\u003e30% isopropanol for ≥ 20 mins\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage buffer\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1x PBS containing 20% ethanol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProduct is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e   \u003cimg alt=\"Keep refrigerated\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Keep-refrigerated_95fa1867-1f0d-47e6-823a-8a0008076e21_small.png?12332456762384261517\" style=\"float: none;\"\u003e   \u003cimg alt=\"Do not freeze\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Do-not-freeze_2_small.png?12332456762384261517\" style=\"float: none;\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eDocuments\u003c\/h5\u003e\n\u003ch4\u003eProduct Information Sheet\u003c\/h4\u003e\n\u003cp\u003e🗎    \u003ca title=\"Man_11-0228_Chemical-Tolerant Ni-Penta Agarose6FF Prepacked Cartridges_v1603.pdf\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Man_11-0229_Chemical-Tolerant_Ni-Penta_Agarose_6_FF_Prepacked_Cartridge_v1603.pdf?9251540950763704712\" rel=\"noopener noreferrer\" target=\"_blank\"\u003eNi-Penta™ Agarose 6FF, Prepacked Cartridges\u003c\/a\u003e\u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"1 mL Columns, Pack of 5","offer_id":41405565042731,"sku":"11-0229-5x1ML","price":177.0,"currency_code":"USD","in_stock":true},{"title":"1 mL Columns, Pack of 5 with 1 Connector","offer_id":41405559537707,"sku":"11-0229-5x1MLB","price":204.0,"currency_code":"USD","in_stock":true},{"title":"5 mL Column, Pack of 1","offer_id":41405388554283,"sku":"11-0229-1x5ML","price":151.0,"currency_code":"USD","in_stock":true},{"title":"5 mL Column, Pack of 1,  with 1 Connector","offer_id":41405562585131,"sku":"11-0229-1x5MLB","price":179.0,"currency_code":"USD","in_stock":true},{"title":"5 mL Columns, Pack of 5","offer_id":41405565173803,"sku":"11-0229-5x5ML","price":586.0,"currency_code":"USD","in_stock":true},{"title":"5 mL Columns, Pack of 5, with 1 Connector","offer_id":41405388619819,"sku":"11-0229-5x5MLB","price":613.0,"currency_code":"USD","in_stock":true},{"title":"16\/10 Column, 20 mL","offer_id":42112519569451,"sku":"11-0229-C20","price":709.0,"currency_code":"USD","in_stock":true},{"title":"7\/10 Columns, 4.7 mL, Pack of 2","offer_id":42112598048811,"sku":"11-0229-C47","price":544.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/11-0229_Ni-Penta_cartridges.png?v=1493997900"},{"product_id":"proteindex-ni-nta-agarose","title":"PROTEINDEX™ HiBond™ Ni-NTA Agarose","description":"\u003cp\u003e\u003cstrong\u003ePROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e™ HiBond™ Ni-NTA Agarose \u003c\/strong\u003eutilizes 4% agarose as the matrix support, and is functionalized with the chelate ligand Nitilotriacetic Acid (NTA), which is charged with nickel ions (Ni\u003csup\u003e2+\u003c\/sup\u003e) to form a very stable octahedral structure with the nickel ion in the center. The structure of Ni-NTA complex is compatible with solutions that contain certain concentration of reducing agents, denaturing agents, detergents and other additives.\u003c\/p\u003e\n\u003cp\u003ePROTEINDEX™ HiBond™ Ni-NTA Agarose can be used to purify 6xHis-tagged proteins expressed in various hosts, such as\u003cem\u003e E.coli\u003c\/em\u003e., yeast, insect cells and mammalian cells.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eProduct Highlights\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eSuited for routine purification of histidine-tagged proteins\u003c\/li\u003e\n\u003cli\u003eHigh yield (typically \u0026gt; 40 mg protein \/mL resins)\u003c\/li\u003e\n\u003cli\u003eLow leakage of nickel ions. Can be re-used for up to 8 preps*\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cspan style=\"color: #666666;\"\u003e*Note: No significant decrease in yield after 8 repetitive uses of resin when recommended buffers are used. Refer to Product Details for compatible reagents.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eApplications\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eManual batch or gravity flow purification\u003c\/li\u003e\n\u003cli\u003eRoutine His tag protein purification for structural biology and functional assays\u003c\/li\u003e\n\u003cli\u003eProtein-protein and protein-nucleic acid interaction studies\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003e\u003c\/span\u003e\u003c\/h4\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eTech Notes\u003c\/span\u003e\u003c\/h4\u003e\n\u003cp\u003e\u003cb\u003eOne-step purification using \u003c\/b\u003e\u003cb\u003eP\u003c\/b\u003e\u003cb\u003eroteIndex\u003c\/b\u003e\u003cb\u003e™ \u003c\/b\u003e\u003cb\u003eHiBond\u003c\/b\u003e\u003cb\u003e™ Ni-NTA \u003c\/b\u003e\u003cb\u003eAgarose 6 Fast Flow resins \u003c\/b\u003e\u003c\/p\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 0px; border-color: #ffffff;\" width=\"30%\"\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Ni-NTA_2.png?v=1496932129\" alt=\"\"\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 0px; border-color: #ffffff;\" width=\"30%\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cstrong\u003eFigure 1. \u003c\/strong\u003eE.coli lysate containing a 50 kDa target protein was directly loaded on HiBond™ Ni-NTA Agarose 6 FF column. The column was then washed sequentially with 0 mM, 5 mM, and 20 mM imidazole solution before elution with 300 mM imidazole. The target protein was analyzed by SDS-PAGE. \u003cb\u003eC\u003c\/b\u003e\u003cb\u003eL\u003c\/b\u003e: raw lysate; \u003cb\u003eFT\u003c\/b\u003e: unbound flow-through; \u003cb\u003eW1\u003c\/b\u003e,\u003cb\u003e W2 \u003c\/b\u003eand \u003cb\u003eW3: \u003c\/b\u003ewashes; \u003cb\u003eE1\u003c\/b\u003e and \u003cb\u003eE2\u003c\/b\u003e: eluate fractions.\n\u003cul\u003e\u003c\/ul\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"200\"\u003e\u003cstrong\u003eLigand\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eNickel\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBinding capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;40 mg 6xHis-tagged protein\/mL medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMatrix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e4% agarose supplied as 50% slurry\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eParticle size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e45 μm - 165 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaximum pressure\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.1 MPa (1 bar)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eRecommended flow rate\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003eGravity flow, \u0026lt;75 cm\/hour\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eChemical compatibility\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003ctable width=\"93%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"37%\" valign=\"top\"\u003eReducing agents\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e5 mM DTE\u003cbr\u003e0.5-1mM DTT\u003cbr\u003e20 mM β-mercaptoethanol\u003cbr\u003e5 mM TCEP\u003cbr\u003e10 mM reduced glutathione (GSH)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"37%\" valign=\"top\"\u003eDenaturing agents\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e8 M urea, 6 M Gua-HCl \u003cbr\u003e(Tested for one week at 40°C)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"37%\" valign=\"top\"\u003eDetergents\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e2% Triton™ X-100 (nonionic)\u003cbr\u003e2% Tween™ 20 (nonionic)\u003cbr\u003e2% NP-40 (nonionic)\u003cbr\u003e2% cholate (anionic)\u003cbr\u003e1% CHAPS (zwitterionic)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"37%\" valign=\"top\"\u003eOther additives\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e500 mM imidazole\u003cbr\u003e20% ethanol\u003cbr\u003e50% glycerol\u003cbr\u003e100 mM Na\u003csub\u003e2\u003c\/sub\u003eSO4\u003cbr\u003e1.5 M NaCl\u003cbr\u003e1 mM EDTA\u003cbr\u003e60 mM citrate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"37%\" valign=\"top\"\u003eCommonly used buffer\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e50 mM sodium phosphate, pH 7.4\u003cbr\u003e100 mM Tris-HCl, pH 7.4\u003cbr\u003e100 mM Tris-acetate, pH 7.4\u003cbr\u003e100 mM HEPES, pH 7.4\u003cbr\u003e100 mM MOPS, pH 7.4\u003cbr\u003e100 mM sodium acetate, pH 4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage buffer\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1x PBS containing 20% ethanol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProduct is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e   \u003cimg alt=\"Keep refrigerated\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Keep-refrigerated_95fa1867-1f0d-47e6-823a-8a0008076e21_small.png?12332456762384261517\" style=\"float: none;\"\u003e   \u003cimg alt=\"Do not freeze\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Do-not-freeze_2_small.png?12332456762384261517\" style=\"float: none;\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003e\u003c\/h5\u003e\n\u003ch4 style=\"color: #ee8012;\"\u003e\u003c\/h4\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"10 mL","offer_id":18927593668,"sku":"11-0224-010","price":115.0,"currency_code":"USD","in_stock":true},{"title":"50 mL","offer_id":18927593732,"sku":"11-0224-050","price":412.0,"currency_code":"USD","in_stock":true},{"title":"100 mL","offer_id":18927593796,"sku":"11-0224-100","price":759.0,"currency_code":"USD","in_stock":true},{"title":"500 mL","offer_id":39291740979243,"sku":"11-0224-500","price":2733.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/11-0224_Ni-NTA.png?v=1483498777"},{"product_id":"proteindex-ni-nta-agarose-6ff","title":"PROTEINDEX™ HiBond™ Ni-NTA Agarose 6FF","description":"\u003cp\u003e\u003cstrong\u003ePROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e\u003c\/strong\u003e™ \u003cb\u003eHiBond\u003c\/b\u003e\u003cb\u003e™ \u003c\/b\u003e\u003cstrong\u003eNi-NTA Agarose\u003c\/strong\u003e \u003cstrong\u003e6FF\u003c\/strong\u003e has been optimized for automated purification applications. It has been widely used in scale-up and preparative purification of histidine-tagged proteins that are expressed in various hosts, such as \u003cem\u003eE.coli\u003c\/em\u003e, yeast, insect cells, and mammalian cells.\u003c\/p\u003e\n\u003cp\u003e        The base matrix of PROTEINDEX™ HiBond™ Ni-NTA Agarose 6FF is highly crosslinked 6% agarose, offering excellent chemical-, and mechanical stability for automated applications. The matrix support is functionalized with the chelate ligand Nitilotriacetic Acid (NTA) in complex with the nickel ions (Ni\u003csup\u003e2+\u003c\/sup\u003e) in a very stable octahedral structure. The structure of Ni-NTA complex is compatible solutions that contain certain concentration of reducing agents, denaturing agents, detergents and other additives.\u003c\/p\u003e\n\u003cp\u003e        The NTA ligand coordinates the Ni\u003csup\u003e2+\u003c\/sup\u003e with four valencies (tetradentate, coordination number 4), and two valencies are available for interaction with imidazole rings of histidine residues. The number of available valencies seems to provide the best combination of binding effectiveness and chemical stability for binding with the His tag. The Ni-NTA ion-chelate complex on our ProteIndex™ HiBond™ Ni-NTA resins has been delicately optimized to ensure the most effective capture for His tag purification of His-tagged proteins. \u003c\/p\u003e\n\u003cp\u003e       \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eProduct Highlights\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eMechanical stable, designed for scaling up His tag purification\u003c\/li\u003e\n\u003cli\u003eHigh yield (typically \u0026gt; 40 mg protein \/mL resins)\u003c\/li\u003e\n\u003cli\u003eLow leakage of nickel ions. Can be re-used for up to 8 preps*\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cspan style=\"color: #666666;\"\u003e*Note: No significant decrease in yield after 8 repetitive uses of resin when recommended buffers are used. Refer to Product Details for compatible reagents.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eApplications\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eAutomated or syringe operations; and manual batch or gravity flow\u003c\/li\u003e\n\u003cli\u003eRoutine His tag protein purification for structural biology and functional assays\u003c\/li\u003e\n\u003cli\u003eScaling up and preparative applications\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eTech Notes\u003c\/span\u003e\u003c\/h4\u003e\n\u003cp\u003e\u003cb\u003eOne-step purification using \u003c\/b\u003e\u003cb\u003eP\u003c\/b\u003e\u003cb\u003eroteIndex\u003c\/b\u003e\u003cb\u003e™ \u003c\/b\u003e\u003cb\u003eHiBond\u003c\/b\u003e\u003cb\u003e™ Ni-NTA \u003c\/b\u003e\u003cb\u003eAgarose 6 Fast Flow resins \u003c\/b\u003e\u003c\/p\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 0px; border-color: #ffffff;\" width=\"35%\"\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Ni-NTA_6FF_2.png?v=1496201543\" alt=\"\" style=\"display: block; margin-left: auto; margin-right: auto;\"\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 0px; border-color: #ffffff;\" width=\"35%\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cstrong\u003eFigure 1.\u003c\/strong\u003e A 15 kDa protein was expressed in E.coli and purified on an automated system using \u003cb\u003eP\u003c\/b\u003e\u003cb\u003eroteIndex\u003c\/b\u003e\u003cb\u003e™ \u003c\/b\u003e\u003cb\u003eHiBond\u003c\/b\u003e\u003cb\u003e™ Ni-NTA \u003c\/b\u003e\u003cb\u003eAgarose 6 Fast Flow resin \u003c\/b\u003eunder native conditions. 50 mL E.coli lysate in 50 mM NaH\u003csub\u003e2\u003c\/sub\u003ePO\u003csub\u003e4\u003c\/sub\u003e, 0.3 M NaCl, 10 mM imidazole was loaded on the column packed with 0.5 mL resins at the speed of 1 mL\/min. The column was then washed with 100 mL 50 mM NaH\u003csub\u003e2\u003c\/sub\u003ePO\u003csub\u003e4\u003c\/sub\u003e, 0.3 M NaCl, 20 mM imidazole at the speed of 1~2 mL\/min. The target protein was eluted with 50 mM NaH\u003csub\u003e2\u003c\/sub\u003ePO\u003csub\u003e4\u003c\/sub\u003e, 0.3 M NaCl, 250 mM imidazole. Proteins were visualized by coomassie blue staining after separation on SDS-PAGE. \u003cb\u003eL\u003c\/b\u003e: raw lysate; \u003cb\u003eF\u003c\/b\u003e: unbound flow-through; \u003cb\u003eE1\u003c\/b\u003e and \u003cb\u003eE2\u003c\/b\u003e: eluate fractions.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 0px; border-color: #ffffff;\" width=\"37%\"\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Ni-NTA_6FF_comparison_with_Ni_sepharose_4.png?v=1497317795\" alt=\"\"\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 0px; border-color: #ffffff;\" width=\"37%\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cstrong\u003eFigure 2.\u003c\/strong\u003e A 25 kDa protein was expressed in E.coli and purified on an automated system using a one-step automated protocol with \u003cb\u003eP\u003c\/b\u003e\u003cb\u003eroteIndex\u003c\/b\u003e\u003cb\u003e™ \u003c\/b\u003e\u003cb\u003eHiBond\u003c\/b\u003e\u003cb\u003e™ Ni-NTA \u003c\/b\u003e\u003cb\u003eAgarose 6 Fast Flow resin \u003c\/b\u003eunder native conditions. 50 mL E.coli lysate in 50 mM NaH\u003csub\u003e2\u003c\/sub\u003ePO\u003csub\u003e4\u003c\/sub\u003e, 0.3 M NaCl, 10 mM imidazole was loaded on the column packed with 0.5 mL resins at the speed of 1 mL\/min. The column was then washed with 100 mL 50 mM NaH\u003csub\u003e2\u003c\/sub\u003ePO\u003csub\u003e4\u003c\/sub\u003e, 0.3 M NaCl, 20 mM imidazole at the speed of 1~2 mL\/min. The target protein was eluted with 50 mM NaH\u003csub\u003e2\u003c\/sub\u003ePO\u003csub\u003e4\u003c\/sub\u003e, 0.3 M NaCl, 250 mM imidazole. A fraction of eluate was analyzed on a SDS-PAGE. Shown were coomassie blue stains of the target proteins purified from columns of Marvelgent’s ProteIndex Ni-NTA 6FF (\u003cstrong\u003eM\u003c\/strong\u003e) and Ni-Sepharose (\u003cstrong\u003eG\u003c\/strong\u003e) resins.\u003cbr\u003e\u003c\/p\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"200\"\u003e\u003cstrong\u003eLigand\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eNickel\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBinding capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u0026gt; 40 mg 6xHis-tagged protein\/mL medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMatrix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e6% highly cross-linked agarose supplied as 50% slurry\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eParticle size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e45 μm - 165 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaximum pressure\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.3 MPa (3 bar)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eChemical compatibility\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003ctable width=\"93%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"37%\" valign=\"top\"\u003eReducing agents\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e5 mM DTE\u003cbr\u003e0.5-1 mM DTT\u003cbr\u003e20 mM β-mercaptoethanol\u003cbr\u003e5 mM TCEP\u003cbr\u003e10 mM reduced glutathione (GSH)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"37%\" valign=\"top\"\u003eDenaturing agents\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e8 M urea, 6 M Gua-HCl \u003cbr\u003e(Tested for one week at 40°C)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"37%\" valign=\"top\"\u003eDetergents\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e2% Triton™ X-100 (nonionic)\u003cbr\u003e2% Tween™ 20 (nonionic)\u003cbr\u003e2% NP-40 (nonionic)\u003cbr\u003e2% cholate (anionic)\u003cbr\u003e1% CHAPS (zwitterionic)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"37%\" valign=\"top\"\u003eOther additives\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e500 mM imidazole\u003cbr\u003e20% ethanol\u003cbr\u003e50% glycerol\u003cbr\u003e100 mM Na\u003csub\u003e2\u003c\/sub\u003eSO4\u003cbr\u003e1.5 M NaCl\u003cbr\u003e1 mM EDTA\u003cbr\u003e60 mM citrate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"37%\" valign=\"top\"\u003eCommonly used buffer\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e50 mM sodium phosphate, pH 7.4\u003cbr\u003e100 mM Tris-HCl, pH 7.4\u003cbr\u003e100 mM Tris-acetate, pH 7.4\u003cbr\u003e100 mM HEPES, pH 7.4\u003cbr\u003e100 mM MOPS, pH 7.4\u003cbr\u003e100 mM sodium acetate, pH 4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage buffer\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1x PBS containing 20% ethanol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProduct is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e   \u003cimg alt=\"Keep refrigerated\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Keep-refrigerated_95fa1867-1f0d-47e6-823a-8a0008076e21_small.png?12332456762384261517\" style=\"float: none;\"\u003e   \u003cimg alt=\"Do not freeze\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Do-not-freeze_2_small.png?12332456762384261517\" style=\"float: none;\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003e\u003cbr\u003e\u003c\/h5\u003e\n\u003ch4 style=\"color: #ee8012;\"\u003e\u003cbr\u003e\u003c\/h4\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"10 mL","offer_id":19000297540,"sku":"11-0225-010","price":126.0,"currency_code":"USD","in_stock":true},{"title":"50 mL","offer_id":19000297604,"sku":"11-0225-050","price":506.0,"currency_code":"USD","in_stock":true},{"title":"100 mL","offer_id":19000297668,"sku":"11-0225-100","price":825.0,"currency_code":"USD","in_stock":true},{"title":"500 mL","offer_id":5704109129767,"sku":"11-0225-500","price":3366.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/11-0225_Ni-NTA_6FF.png?v=1496933028"},{"product_id":"proteindex-ni-nta-agarose-6ff-prepacked-cartridge","title":"PROTEINDEX™ HiBond™ Ni-NTA Agarose 6FF, Prepacked Cartridge","description":"\u003cp\u003e\u003cstrong\u003ePROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e\u003c\/strong\u003e™ \u003cb\u003eHiBond\u003c\/b\u003e\u003cb\u003e™ \u003c\/b\u003e\u003cstrong\u003eNi-NTA Agarose\u003c\/strong\u003e \u003cstrong\u003e6FF\u003c\/strong\u003e has been optimized for automated purification applications. It has been widely used in scale-up and preparative purification of histidine-tagged proteins that are expressed in various hosts, such as \u003cem\u003eE.coli\u003c\/em\u003e, yeast, insect cells, and mammalian cells.\u003c\/p\u003e\n\u003cp\u003e        The base matrix of PROTEINDEX™ HiBond™ Ni-NTA Agarose 6FF is highly crosslinked 6% agarose, offering excellent chemical-, and mechanical stability for automated applications. The matrix support is functionalized with the chelate ligand Nitilotriacetic Acid (NTA) in complex with the nickel ions (Ni\u003csup\u003e2+\u003c\/sup\u003e) in a very stable octahedral structure. The structure of Ni-NTA complex is compatible solutions that contain certain concentration of reducing agents, denaturing agents, detergents and other additives.\u003c\/p\u003e\n\u003cp\u003e        The NTA ligand coordinates the Ni\u003csup\u003e2+\u003c\/sup\u003e with four valencies (tetradentate, coordination number 4), and two valencies are available for interaction with imidazole rings of histidine residues. The number of available valencies seems to provide the best combination of binding effectiveness and chemical stability for binding with the His tag. The Ni-NTA ion-chelate complex on our ProteIndex™ HiBond™ Ni-NTA resins has been delicately optimized to ensure the most effective capture for His tag purification of His-tagged proteins. \u003c\/p\u003e\n\u003cp\u003e        The Prepacked Cartridge has standard interface and can be adapted to most commonly used chromatography systems, such as ÄKTA systems.\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e        Value bundle with a \u003c\/span\u003e\u003ca rel=\"noopener\" title=\"Connector Luer Female \/ 10-32 Male in PEEK\" href=\"https:\/\/marvelgent.com\/products\/connector-1-16-luer-female-10-32-male-peek\" target=\"_blank\"\u003eLuer Female\/10-32 Male Connector in PEEK\u003c\/a\u003e\u003cspan\u003e (Cat# 20-0315) is now available for all pack sizes, providing convenient operation of the pre-packed column with a syringe at an excellent value.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eProduct Highlights\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eConvenient, easy, ready-to-use\u003c\/li\u003e\n\u003cli\u003eMechanical stable, designed for large scale purification of histidine-tagged proteins\u003c\/li\u003e\n\u003cli\u003eHigh yield (typically \u0026gt; 40 mg protein \/mL resins)\u003c\/li\u003e\n\u003cli\u003eLow leakage of nickel ions. Can be re-used for up to 8 preps*\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cspan style=\"color: #666666;\"\u003e*Note: No significant decrease in yield after 8 repetitive uses of resin when recommended buffers are used. Refer to Product Details for compatible reagents.\u003c\/span\u003e\u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eApplications\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eAutomated purifications\u003c\/li\u003e\n\u003cli\u003eRoutine His tag protein purification for structural biology and functional assays\u003c\/li\u003e\n\u003cli\u003eScaling up and preparative applications\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eTech Notes\u003c\/span\u003e\u003c\/h4\u003e\n\u003cp\u003e\u003cb\u003eOne-step purification using \u003c\/b\u003e\u003cb\u003eP\u003c\/b\u003e\u003cb\u003eroteIndex\u003c\/b\u003e\u003cb\u003e™ \u003c\/b\u003e\u003cb\u003eHiBond\u003c\/b\u003e\u003cb\u003e™ Ni-NTA \u003c\/b\u003e\u003cb\u003eAgarose 6 Fast Flow resins \u003c\/b\u003e\u003c\/p\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"35%\" style=\"border: 0px; border-color: #ffffff;\"\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Ni-NTA_6FF_2.png?v=1496201543\"\u003e\u003c\/td\u003e\n\u003ctd width=\"35%\" style=\"border: 0px; border-color: #ffffff;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cstrong\u003eFigure 1.\u003c\/strong\u003e A 15 kDa protein was expressed in E.coli and purified on an automated system using \u003cb\u003eP\u003c\/b\u003e\u003cb\u003eroteIndex\u003c\/b\u003e\u003cb\u003e™ \u003c\/b\u003e\u003cb\u003eHiBond\u003c\/b\u003e\u003cb\u003e™ Ni-NTA \u003c\/b\u003e\u003cb\u003eAgarose 6 Fast Flow resin \u003c\/b\u003eunder native conditions. 50 mL E.coli lysate in 50 mM NaH\u003csub\u003e2\u003c\/sub\u003ePO\u003csub\u003e4\u003c\/sub\u003e, 0.3 M NaCl, 10 mM imidazole was loaded on the column packed with 0.5 mL resins at the speed of 1 mL\/min. The column was then washed with 100 mL 50 mM NaH\u003csub\u003e2\u003c\/sub\u003ePO\u003csub\u003e4\u003c\/sub\u003e, 0.3 M NaCl, 20 mM imidazole at the speed of 1~2 mL\/min. The target protein was eluted with 50 mM NaH\u003csub\u003e2\u003c\/sub\u003ePO\u003csub\u003e4\u003c\/sub\u003e, 0.3 M NaCl, 250 mM imidazole. Proteins were visualized by coomassie blue staining after separation on SDS-PAGE. \u003cb\u003eL\u003c\/b\u003e: raw lysate; \u003cb\u003eF\u003c\/b\u003e: unbound flow-through; \u003cb\u003eE1\u003c\/b\u003e and \u003cb\u003eE2\u003c\/b\u003e: eluate fractions.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"37%\" style=\"border: 0px; border-color: #ffffff;\"\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Ni-NTA_6FF_comparison_with_Ni_sepharose_4.png?v=1497317795\"\u003e\u003c\/td\u003e\n\u003ctd width=\"37%\" style=\"border: 0px; border-color: #ffffff;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cstrong\u003eFigure 2.\u003c\/strong\u003e A 25 kDa protein was expressed in E.coli and purified on an automated system using a one-step automated protocol with \u003cb\u003eP\u003c\/b\u003e\u003cb\u003eroteIndex\u003c\/b\u003e\u003cb\u003e™ \u003c\/b\u003e\u003cb\u003eHiBond\u003c\/b\u003e\u003cb\u003e™ Ni-NTA \u003c\/b\u003e\u003cb\u003eAgarose 6 Fast Flow resin \u003c\/b\u003eunder native conditions. 50 mL E.coli lysate in 50 mM NaH\u003csub\u003e2\u003c\/sub\u003ePO\u003csub\u003e4\u003c\/sub\u003e, 0.3 M NaCl, 10 mM imidazole was loaded on the column packed with 0.5 mL resins at the speed of 1 mL\/min. The column was then washed with 100 mL 50 mM NaH\u003csub\u003e2\u003c\/sub\u003ePO\u003csub\u003e4\u003c\/sub\u003e, 0.3 M NaCl, 20 mM imidazole at the speed of 1~2 mL\/min. The target protein was eluted with 50 mM NaH\u003csub\u003e2\u003c\/sub\u003ePO\u003csub\u003e4\u003c\/sub\u003e, 0.3 M NaCl, 250 mM imidazole. A fraction of eluate was analyzed on a SDS-PAGE. Shown were coomassie blue stains of the target proteins purified from columns of Marvelgent’s ProteIndex Ni-NTA 6FF (\u003cstrong\u003eM\u003c\/strong\u003e) and Ni-Sepharose (\u003cstrong\u003eG\u003c\/strong\u003e) resins.\u003c\/p\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"200\"\u003e\u003cstrong\u003eLigand\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eNickel\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBinding capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;40 mg 6xHis-tagged protein\/mL medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMatrix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e6% highly cross-linked agarose\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eParticle size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e45 μm - 165 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eColumn volume\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1 mL or 5 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaximum pressure\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.3 MPa (3 bar)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eChemical compatibility\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003ctable width=\"93%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"37%\"\u003eReducing agents\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e5 mM DTE\u003cbr\u003e0.5-1 mM DTT\u003cbr\u003e20 mM β-mercaptoethanol\u003cbr\u003e5 mM TCEP\u003cbr\u003e10 mM reduced glutathione (GSH)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"37%\"\u003eDenaturing agents\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e8 M urea, 6 M Gua-HCl \u003cbr\u003e(Tested for one week at 40°C)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"37%\"\u003eDetergents\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e2% Triton™ X-100 (nonionic)\u003cbr\u003e2% Tween™ 20 (nonionic)\u003cbr\u003e2% NP-40 (nonionic)\u003cbr\u003e2% cholate (anionic)\u003cbr\u003e1% CHAPS (zwitterionic)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"37%\"\u003eOther additives\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e500 mM imidazole\u003cbr\u003e20% ethanol\u003cbr\u003e50% glycerol\u003cbr\u003e100 mM Na\u003csub\u003e2\u003c\/sub\u003eSO4\u003cbr\u003e1.5 M NaCl\u003cbr\u003e1 mM EDTA\u003cbr\u003e60 mM citrate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"37%\"\u003eCommonly used buffer\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e50 mM sodium phosphate, pH 7.4\u003cbr\u003e100 mM Tris-HCl, pH 7.4\u003cbr\u003e100 mM Tris-acetate, pH 7.4\u003cbr\u003e100 mM HEPES, pH 7.4\u003cbr\u003e100 mM MOPS, pH 7.4\u003cbr\u003e100 mM sodium acetate, pH 4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eColumn hardware pressure limit\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003e5 bar (0.5 MPa)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage buffer\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1x PBS containing 20% ethanol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProduct is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e   \u003cimg style=\"float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Keep-refrigerated_95fa1867-1f0d-47e6-823a-8a0008076e21_small.png?12332456762384261517\" alt=\"Keep refrigerated\"\u003e   \u003cimg style=\"float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Do-not-freeze_2_small.png?12332456762384261517\" alt=\"Do not freeze\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"5x 1 mL Columns","offer_id":19098127684,"sku":"11-0230-5x1ML","price":172.0,"currency_code":"USD","in_stock":true},{"title":"5x 1 mL Columns with 1 Connector","offer_id":41405616259115,"sku":"11-0230-5x1MLB","price":200.0,"currency_code":"USD","in_stock":true},{"title":"1x 5 mL Column","offer_id":19098127748,"sku":"11-0230-1x5ML","price":149.0,"currency_code":"USD","in_stock":true},{"title":"1x 5 mL Column with 1 Connector","offer_id":41405616291883,"sku":"11-0230-1x5MLB","price":177.0,"currency_code":"USD","in_stock":true},{"title":"5x 5mL Columns","offer_id":19098127812,"sku":"11-0230-5x5ML","price":539.0,"currency_code":"USD","in_stock":true},{"title":"5x 5 mL Columns with 1 Connector","offer_id":41405616324651,"sku":"11-0230-5x5MLB","price":566.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/Ni-NTA_6FF_prepacked_columns.png?v=1496933949"},{"product_id":"proteindex-co-penta-agarose-6-fast-flow-chemical-stable","title":"PROTEINDEX™ Co-Penta™ Agarose 6 Fast Flow, Chemical Stable","description":"\u003cp\u003e\u003cstrong\u003ePROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e\u003c\/strong\u003e™ \u003cstrong\u003eChemical-Tolerant Ni-Penta™ Agarose 6FF\u003c\/strong\u003e is a new immobilized metal ion affinity chromatography (IMAC) medium precharged with cobalt ions, designed mainly for purification of low abundant proteins or challenging proteins in conditions where EDTA, DTT, β-ME, and other stabilizing agents are necessary for maintaining protein stability and integrity.\u003c\/p\u003e\n\u003cp\u003eThe cobalt ion binds very strongly to the matrix support in complex with a proprietary pentadentate chelating ligand, which immobilizes the nickel ion through 5 sites to form a homogeneous,  chemical-stable structure. This characteristic nickel ion bonding on the Co-Penta™ resin provides excellent resistance to EDTA, NaOH, β-ME, and other reagents that are restricted in the purification protocol using conventional NTA- and IDA- functionalized resins. In particular, Co-Penta™ Agarose 6FF is well suited for direct capture of secreted polyHis-tagged proteins from the cell culture supernatant of eukaryotic cells, including CHO cells, insect High Five™ cells* and Sf9 cells.\u003c\/p\u003e\n\u003cp\u003eHighly cross-linked 6% agarose as the base matrix support offers excellent mechanical stability for automated, scale up purification of histidine-tagged proteins.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eProduct Highlights\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eEDTA-resistant\u003c\/li\u003e\n\u003cli\u003eHigh selectivity for high purity\u003c\/li\u003e\n\u003cli\u003eRetained high 6xHis affinity in various buffers\u003c\/li\u003e\n\u003cli\u003eRecharge-free with minimal ion leakage\u003c\/li\u003e\n\u003cli\u003eRequires less imidazole\u003c\/li\u003e\n\u003cli\u003eNaOH-tolerant, allowing CIP with 1 M NaOH\u003c\/li\u003e\n\u003cli\u003eVersatile, ready-to-use with convenient formats\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003e\u003cbr\u003e\u003c\/h4\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"200\"\u003e\u003cstrong\u003eLigand\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eCobalt\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBinding capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;10 mg 6x His-tagged protein\/mL medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMatrix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eHighly cross-linked 6% agarose resin supplied as 50% slurry\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eParticle size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e45 μm - 165 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaximum pressure\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.3 MPa (3 bar)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eChemical compatibility\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cul\u003e\n\u003cli\u003e0.01 M HCl ,0.01 M NaOH, for ≥ 1 week;\u003c\/li\u003e\n\u003cli\u003e10 mM EDTA, 1 M NaOH, 5 mM DTT, 5 mM TCEP, 20 mM β-ME, 6 M guanidine-HCl, for ≥ 24 hours\u003c\/li\u003e\n\u003cli\u003e500 mM imidazole，100 mM EDTA, for ≥ 2 hours\u003c\/li\u003e\n\u003cli\u003e30% isopropanol for ≥ 20 mins\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage buffer\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1x PBS containing 20% ethanol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProduct is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e   \u003cimg alt=\"Keep refrigerated\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Keep-refrigerated_95fa1867-1f0d-47e6-823a-8a0008076e21_small.png?12332456762384261517\" style=\"float: none;\"\u003e   \u003cimg alt=\"Do not freeze\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Do-not-freeze_2_small.png?12332456762384261517\" style=\"float: none;\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\n\u003cp\u003e \u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"10 mL","offer_id":42338889498667,"sku":"11-0305-010","price":203.0,"currency_code":"USD","in_stock":true},{"title":"50 mL","offer_id":42338889531435,"sku":"11-0305-050","price":819.0,"currency_code":"USD","in_stock":true},{"title":"100 mL","offer_id":42338889564203,"sku":"11-0305-100","price":1485.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/11-0305Co-Penta6FF_1_52d65e6e-5a67-4887-ac12-f925189652c5.png?v=1759535666"}],"url":"https:\/\/marvelgent.com\/collections\/most-popular-products\/ligand-functional-group_metal-ion-cobalt.oembed","provider":"Marvelgent Biosciences","version":"1.0","type":"link"}