{"title":"Proteases","description":"\u003cp\u003eProteoSure™ family enzymes and proteins are genetically engineered and expressed in E.coli. They are purified via our proprietary ProteoSure™ purification process for the highest specific enzymatic activities. Unlike other commercial enzymes that are derived from an animal source via lengthy extraction and purification process, our ProteoSure™ proteins contain no animal derived impurities and retain their full enzymatic potency. The animal-free recombinant proteins are suitable for a broad range of protein processing and analysis applications. Explore our full list of Enzymatic Tools for Protein Processing and Analysis: \u003c\/p\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 2px solid #eeeeee; background: #eeeeee; width: 47%;\" valign=\"middle\"\u003e\n\u003cul\u003e\n\u003cli style=\"color: #008bc8; padding-left: 18px;\"\u003e\u003ca href=\"https:\/\/marvelgent.com\/collections\/proteases-for-protein-sciences-and-cell-biology\"\u003eProteases for Protein Sciences and Cell Biology\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border: 2px solid #eeeeee; background: #eeeeee; width: 47%;\" valign=\"middle\"\u003e\n\u003cul\u003e\n\u003cli style=\"color: #008bc8; padding-left: 18px;\"\u003e\u003ca href=\"http:\/\/www.marvelgent.com\/collections\/sequencing-grade-enzymes\" title=\"Sequencing-grade enzymes\"\u003eAnalytical Enzymes for Sequencing and Mass Spectrometry\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e","products":[{"product_id":"trypsin-porcine-recombinant","title":"ProteoSure™ Recombinant Porcine Trypsin","description":"\u003cp\u003eTrypsin is a member of the serine protease family. Trypsin cleaves peptides on the C-terminal end of lysine and arginine amino acid residues. The optimum pH of trypsin lies between 7 - 9. The enzyme is inhibited by serine protease inhibitors (e.g. PMSF) and metal chelating agents (e.g. EDTA).\u003c\/p\u003e\n\u003cp\u003eRecombinant Porcine Trypsin is a genetically engineered protein that is expressed in \u003cem\u003eE. coli \u003c\/em\u003eand purified by high pressure liquid chromatography. There is no contaminating enzyme activity from carboxypeptidase A or chymotrypsin. No protease inhibitors such as PMSF are used during the manufacturing process. \u003cbr\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eProduct Highlights\u003cbr\u003e\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cstrong\u003eAnimal origin free\u003c\/strong\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp style=\"padding-left: 60px;\"\u003eThe use of recombinant porcine Trypsin eliminates the potential risk of contamination with virus and any other potential adventitious agents found in animal-derived trypsin. Recombinant Porcine Trypsin 100% animal origin free, and is manufactured in ISO9001 facility.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cstrong\u003eStable\u003c\/strong\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp style=\"padding-left: 60px;\"\u003eRecombinant porcine trypsin is supplied as sterile lyophilized powder. It minimizes the risk of contamination and activity loss during the process of transport and storage.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cstrong\u003eHigh purity\u003c\/strong\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003col\u003e\n\u003col\u003e\n\u003cli\u003eRecombinant porcine trypsin provides super purity and specificity.\u003c\/li\u003e\n\u003cli\u003eNo other contaminating proteases such as chymotrypsin or carboxypeptidase A.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"699\" height=\"297\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"188\"\u003e\u003cstrong\u003eCAS #\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e9002-07-07\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eEnzyme Commission #\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e3.4.21.4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMolecular weight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e23.8 kDa\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eOrigin of species\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003ePorcine\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSource\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cem\u003eE. coli\u003c\/em\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003ePurified by\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eHPLC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003ePhysical form\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003eWhite lyophilized powder\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eAdditives\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003eCarbohydrates\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eProtein content\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003e≥70% β-trypsin, and ≤ 20% α-trypsin\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSpecific activity\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e≥ 3800 USP unit\/mg protein\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"vertical-align: top;\"\u003e\u003cstrong\u003eUnit definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eOne USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a reaction volume of 3.0 mL at pH7.6 and 25℃, with BAEE as a substrate (1 cm light path).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003ePurity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e≥ 99%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eContaminant activity\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eNo chymotrypsin, carboxypeptidase A, or other protease contaminants.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore in a sealed container at 2 - 8°C. Once reconstituted, it should be stored at -20°C. The product is stable for 24 months. Greater than 90% enzymatic activity retains after 10 repeated freeze-and-thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eIntended use\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFor research or processing only. Not intended for any human or animal diagnostic or therapeutic use.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eDocuments\u003c\/h5\u003e\n\u003ch4\u003eProduct Information Sheet\u003c\/h4\u003e\n\u003cp\u003e🗎    Recombinant Porcine Trypsin, 100% Animal Original Free\u003c\/p\u003e\n\u003ch4\u003eTechnical Note\u003c\/h4\u003e\n\u003cp\u003e🗎    \u003ca title=\"Conversion table of enzyme activity measurement units\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Table_Conversion_of_Enzyme_Activity_Measurement_Units.pdf?4497787588428185691\" target=\"_blank\"\u003eConversion of Enzyme Activity Measurement Units\u003c\/a\u003e\u003c\/p\u003e","brand":"YaxinBio","offers":[{"title":"100 mg","offer_id":17783726212,"sku":"14-RPT-100MG","price":655.0,"currency_code":"USD","in_stock":true},{"title":"5x 100 mg","offer_id":15410997444,"sku":"14-RPT-500MG","price":2480.0,"currency_code":"USD","in_stock":true},{"title":"10x 100 mg","offer_id":39659679514667,"sku":"14-RPT-1G","price":3575.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/14-RPT_AOF3.png?v=1493128788"},{"product_id":"trypsin-for-protein-sequencing-and-mass-spectrometry","title":"ProteoSure™ Modified, Recombinant Porcine Trypsin, Autolysis-Resistant for Proteomic Sequencing and Mass Spectrometry Analysis","description":"\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eProduct highlights\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cspan\u003eEnables fast digestion and reduces wait time in sample preparation; \u003c\/span\u003e\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eFree of non-tryptic activities for high quality, unambiguous mass spectral interpretation;\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eProvides enhanced peptide map coverage;\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan\u003eSuits a wide range of proteomics applications.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eProduct overview\u003c\/span\u003e\u003c\/h4\u003e\n\u003cp\u003eTrypsin is a crucial serine protease that specifically cleaves peptide bonds at the carboxyl side of lysine and arginine residues. It is widely utilized in mass spectrometry-based protein analysis and proteomics due to its high proteolytic activity and cleavage specificity.\u003c\/p\u003e\n\u003cp\u003eProteoSure™ Modified, Recombinant Porcine Trypsin is a highly purified recombinant variant of porcine trypsin. It has been genetically optimized and chemically modified to achieve superior resistance to autolysis and maximize specific tryptic activity.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFree from Non-Specific Protease Contaminants\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eMost commercially available proteomic-grade trypsin products are derived from animal pancreatic extracts and may contain traces of chymotrypsin, carboxypeptidase A, and other proteases. Although chymotrypsin activity can be inhibited by treatment with TPCK (L-(tosylamido-2-phenyl) ethyl chloromethyl ketone), the residual activity as well as other contaminants can compromise protein analysis quality and extend digestion times.\u003c\/p\u003e\n\u003cp\u003eProteoSure™ Recombinant Porcine Trypsin is expressed and purified from E. coli in a strictly controlled system, ensuring batch-to-batch consistency. Our recombinant trypsin is free from contaminating protease activities, and it does not require TPCK treatment.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSuperior Autoproteolytic Resistance\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThe unmodified, native trypsin (β-trypsin) is prone to autolysis, forming α-trypsin, which exhibits reduced stability and efficiency. Further degradation of α-trypsin results in pseudotrypsin (ψ-trypsin), showing broad non-specific activities, including chymotrypsin-like activity. These changes compromise the enzyme’s performance, affecting reproducibility in protein digestion and mass spectrometry-based identification.\u003c\/p\u003e\n\u003cp\u003eProteoSure™ Modified, Recombinant Porcine Trypsin is specifically designed to address the autolysis issue. It is a highly purified recombinant porcine trypsin variant that has been genetically improved for enhanced resistance to autoproteolysis.\u003c\/p\u003e\n\u003cp\u003eAdditionally, it undergoes a proprietary reductive methylation process resulting a modified sequence that exhibits significantly enhanced resistance to autoproteolysis, making it superior to other recombinant trypsin products (see below Figure 1).\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eHigh Efficiency and Enhanced Sequence Coverage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eWith its precise tryptic specificity, enhanced resistance to autolysis, and no contaminating proteases, ProteoSure™ Modified, Recombinant Porcine Trypsin can be optimized for protocols that require high enzyme-to-protein ratios, significantly reducing digestion times while providing superior sequence coverage for unambiguous mass spectral analysis (Figure 2). This cost-effective, high-performance trypsin meets the stringent demands of both research and biopharmaceutical analysis, eliminating the need to choose between sequencing-grade and MS-grade trypsin.\u003c\/p\u003e\n\u003cp\u003eProteoSure™ Modified, Recombinant Porcine Trypsin can also be used in combination with \u003ca href=\"https:\/\/marvelgent.com\/products\/proteosure-recombinant-endoproteinase-lys-c-sequencing-grade\" title=\"Lys-C sequencing grade\"\u003eLysyl Endopeptidase (Lys-C)\u003c\/a\u003eto further improve peptide mapping, particularly for complex proteins.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eApplications\u003c\/span\u003e\u003c\/h4\u003e\n\u003cp\u003eIt is suitable for in-solution or in-gel protein digestion in applications:\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003ePeptide sequencing and tryptic mapping\u003c\/li\u003e\n\u003cli\u003eProtein identification by peptide mass fingerprinting or MS\/MS spectral matching.\u003c\/li\u003e\n\u003cli\u003eProtein-structure studies\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eTechnical notes\u003cbr\u003e\u003c\/span\u003e\u003c\/h4\u003e\n\u003cp\u003eProteoSure™ Modified, Recombinant Porcine Trypsin possesses mutation that is dedicatedly optimized for greater autolysis-resistance. Most commonly used MS grade Trypsin proteases are modified from native Trypsin that are derived from porcine or bovine pancreatic extracts. Although modification by reductive methylation significantly reduces autolysis, autolytic products of modified Trypsin are commonly found in digests and show as characteristic peaks in the chromatogram of HPLC. It has been also suggested that these autolytic products are present during the purification process from the pancreas  prior to modification.\u003c\/p\u003e\n\u003cp\u003e        By introducing mutations to the porcine trypsin sequence, the resultant protease variant has obtained much greater autolysis-resistance after modification by reductive methylation at \u003cspan class=\"st\"\u003eLysine residues\u003c\/span\u003e, in comparison with the modified native protease. Shown in Figure 1, after enzymatic digestion incubated with ProteoSure Sequencing Grade Modified Tryspin, the characteristic peaks of the autolysed trypsin fragments are not detectable by RP-HPLC.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/14-SRT_Modified_AdvTrypsin_seq-grade_autolysis_resistant_no_legend.png?v=1492137028\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eFigure 1. Autolysis of the modified native porcine trypsin from Manufacturer P and Marvelgent’sProteoSure™ Modified Recombinant Trypsin.\u003c\/b\u003e The two proteomic grade trypsin proteases were diluted to 0.05 mg\/mL in 50 mM NH4HCO3 (pH 8.0), and then incubated at 37°C. After 3 hours incubation, the protein samples were analyzed by RP-HPLC. In comparison with the protein prior to incubation, the modified trypsin from Manufacturer P has the protein peak shifted from the original position after 3 hour incubation at 37°C, suggesting that autolysis of trypsin occurred and it resulted in smaller fragments of trypsin. However, ProteoSure™ Modified Recombinant Trypsin showed a peak at the same position before and after 3 hour incubation at 37°C, suggesting that the enzyme remained intact. \u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/14-SRT_Modified_AdvTrypsin_peptide_map_full.png?v=1733349125\" alt=\"\"\u003e\u003cimg\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eFigure 2. Coverage maps of two monoclonal antibodies analyzed by LC-MS.\u003c\/b\u003e The mAbs were proteolytically digested for 5 hours respectively with ProteoSure™ Modified, Recombinant Trypsin and that from Supplier P. The peptide sequences digested by ProteoSure™ rTrypsin delivered a coverage rate of 94.9% for mAb002 and 96.7% for mAb007, respectively (A and C). In comparison, the peptide sequences after digestion with Supplier P’s trypsin resulted in 91.9% and 95.8% coverage (B and D).\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eInstructions to use\u003c\/span\u003e\u003c\/h4\u003e\n\u003col\u003e\n\u003cli\u003eDilute the product in 50mM HAc when needed. Immediately before use, dilute it with 50mM NH\u003csub\u003e4\u003c\/sub\u003eHCO\u003csub\u003e3 \u003c\/sub\u003eor a pH7-8 buffer. It is recommended to add 1mM CaCl\u003csub\u003e2\u003c\/sub\u003e to the reaction. The ratio of Trypsin to the protein sample should be between 1:20 and 1:100 (w\/w). Keep the conditions within the optimum pH of Trypsin (pH7.8 - 8.7).\u003c\/li\u003e\n\u003cli\u003eThaw a frozen aliquot of Trypsin solution at room temperature, and gently mix before use. \u003c\/li\u003e\n\u003cli\u003eWhen 0.05 mg\/ml modified recombinant trypsin is used in buffer 50mM NH\u003csub\u003e4\u003c\/sub\u003eHCO\u003csub\u003e3\u003c\/sub\u003e, it retains greater than 95% activity after 3 hours incubation at 37°C. If longer digestion is desired, such as 20 hour incubation at 37°C, it is recommended to include 1mM CaCl\u003csub\u003e2\u003c\/sub\u003e to the reaction.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eRecommended storage\u003cbr\u003e\u003c\/span\u003e\u003c\/h4\u003e\n\u003col\u003e\n\u003cli\u003eStore the product as lyophilized powder in a sealed container at 2 - 8°C. The product can also be stored at -10 to -20°C and is stable for up to 24 months.\u003c\/li\u003e\n\u003cli\u003eOnce reconstituted, aliquots of the product should be stored at -20°C. The enzyme is stable for up to a year. The solution can also be stored at -70°C or below. It is stable within 24 months.\u003c\/li\u003e\n\u003cli\u003eGreater than 95% enzymatic activity retains after 5 repeated freezing-and-thawing cycles.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"699\" height=\"297\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"188\" style=\"width: 188px;\"\u003e\u003cstrong\u003eCAS #\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 346.4px;\"\u003e9002-07-07\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 188px;\"\u003e\u003cstrong\u003eEnzyme Commission #\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 346.4px;\"\u003e3.4.21.4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 188px;\"\u003e\u003cstrong\u003eMolecular weight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 346.4px;\"\u003e23.8 kDa\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 188px;\"\u003e\u003cstrong\u003eOrigin of species\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 346.4px;\"\u003ePorcine\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 188px;\"\u003e\u003cstrong\u003eSource\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 346.4px;\"\u003e\u003cem\u003eE.coli\u003c\/em\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 188px;\"\u003e\u003cstrong\u003eOptimum pH\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 346.4px;\"\u003e7.8-8.7\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 188px;\"\u003e\u003cstrong\u003ePurified by\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 346.4px;\"\u003eHPLC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 188px;\"\u003e\u003cstrong\u003ePhysical form\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 346.4px;\"\u003eLyophilized powder. \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" style=\"width: 188px;\"\u003e\u003cstrong\u003eSpecific activity\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 346.4px;\"\u003e≥4500 USP \/mg protein, autolytic activity minimized by reductive methylation.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"vertical-align: top; width: 188px;\"\u003e\u003cstrong\u003eUnit definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 346.4px;\"\u003eOne USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a reaction volume of 3.0 mL at pH7.6 and 25℃, with BAEE as a substrate (1 cm light path).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 188px;\"\u003e\u003cstrong\u003ePurity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 346.4px;\"\u003e≥95% by HPLC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 188px;\"\u003e\u003cstrong\u003eContaminant activity\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 346.4px;\"\u003eNo chymotrypsin, carboxypeptidase A, or other protease contaminants.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" style=\"width: 188px;\"\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 346.4px;\"\u003eStore the product as the lyophilized powder in a sealed container at 2 - 8°C. Reconstitute the lyophilized enzyme powder in 1 mM HCl or 50 mM acetic acid to make a stock solution of 5 mg\/mL. Once reconstituted, aliquots of the product should be stored at -70°C. It is stable within 24 months. Greater than 95% enzymatic activity retains after 5 repeated freezing-and-thawing cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" style=\"width: 188px;\"\u003e\u003cstrong\u003eIntended use\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 346.4px;\"\u003eFor research only. Not intended for any human or animal diagnostic or therapeutic use.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eDocuments\u003c\/h5\u003e\n\u003ch4\u003eProduct Information Sheet\u003c\/h4\u003e\n\u003cp\u003e🗎     Modified, Engineered Porcine Trypsin, Sequencing-Grade, Autolysis-resistant\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ch4\u003eTechnical Note\u003c\/h4\u003e\n\u003cp\u003e🗎     \u003ca title=\"Conversion table of enzyme activity measurement units\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Table_Conversion_of_Enzyme_Activity_Measurement_Units.pdf?4497787588428185691\" target=\"_blank\"\u003eConversion of Enzyme Activity Measurement Units\u003c\/a\u003e\u003c\/p\u003e","brand":"Marvelgent Biosciences","offers":[{"title":"2x 100 μg","offer_id":16288514564,"sku":"14-SRT-200UG","price":275.0,"currency_code":"USD","in_stock":true},{"title":"5x 100 μg","offer_id":16288514628,"sku":"14-SRT-500UG","price":594.0,"currency_code":"USD","in_stock":true},{"title":"10x 100 μg","offer_id":39647704088619,"sku":"14-SRT-1MG","price":1072.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/14-SRT_Modified_AdvTrypsin_seq-grade.png?v=1492051336"},{"product_id":"carboxypeptidase-b-proteomics-sequencing-ms","title":"ProteoSure™ Recombinant Carboxypeptidase B, for Proteomic Sequencing and Mass Spectrometry Analysis","description":"\u003cp\u003eCarboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides. The molecular weight is 34,500 daltons, optimum pH is 8.0, and pI is 6.0. Carboxypeptidase B is competitively inhibited by arginine and lysine. The enzyme is also inhibited by metal chelating agents, e.g., EDTA. Recombinant Carboxypeptidase B is expressed in \u003cem\u003eE.coli\u003c\/em\u003e and purified by high pressure liquid chromatography. There is no trace of other enzyme (such as carboxypeptidase A and chymotrypsin) activity. No protease inhibitors (such as PMSF) are used during the manufacturing process.\u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eApplication\u003c\/span\u003e\u003c\/h4\u003e\n\u003col\u003e\n\u003cli\u003eProtein structure and sequence analysis, such as hydrolyze basic amino acids lysine, arginine and histidine from the C-terminal of polypeptides.\u003c\/li\u003e\n\u003cli\u003eAntibody quality control.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eRecommended usage\u003c\/span\u003e\u003c\/h4\u003e\n\u003cp style=\"padding-left: 30px;\"\u003ePrepare 1-10mg\/mL carboxypeptidase B with sterile water or 25 mM Tris-HCl pH 7.5. The recommended ratio to the sample protein is 1:50 to 1:1000 (w\/w), the optimum pH is pH 7 - 9.\u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eStorage and stability\u003c\/span\u003e\u003c\/h4\u003e\n\u003cdiv style=\"padding-left: 30px;\"\u003eStore recombinant carboxypeptidase B lyophilized powder in a sealed container at 2-8°C. It is stable for 24 months.\u003c\/div\u003e\n\u003cdiv style=\"padding-left: 30px;\"\u003eAfter dissolved, it should be stored at -20°C. It is stable for 24 months with no detectable loss of enzymatic activity after 10 repeated freezing-and-thawing cycles.\u003c\/div\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"699\" height=\"297\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"188\"\u003e\u003cstrong\u003eCAS #\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e9025-24-5\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eEnzyme Commission #\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e3.4.17.2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMolecular weight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e34.5 kDa\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eOrigin\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eRat\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSource\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cem\u003eE.coli\u003c\/em\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003ePurified by\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eHPLC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003ePhysical form\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003eLyophilized powder\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSpecific activity\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e≥200 units\/mg protein\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"vertical-align: top;\"\u003e\u003cstrong\u003eUnit definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eOne Unit of carboxypeptidase B activity hydrolyzes one micromole of hippuryl-L-arginine per minute at 25°C, pH 7.65.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eContaminant activity\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eNo trypsin, chymotrypsin, carboxypeptidase A, or other protease contaminants.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore in a sealed container at 2 - 8°C. Once reconstituted, it should be aliquoted and stored at -20°C. The product is stable for 24 months with no detectable loss of enzymatic activity after 10 repeated freeze-and-thaw cycles.  \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eIntended use\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFor research only. Not intended for any human or animal diagnostic or therapeutic use.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eDocuments\u003c\/h5\u003e\n\u003ch4\u003eProduct Information Sheet\u003c\/h4\u003e\n\u003cp\u003e🗎    Recombinant Rat Carboxypeptidase B, Sequencing Grade\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ch4\u003eTechnical Note\u003c\/h4\u003e\n\u003cp\u003e🗎    \u003ca title=\"Conversion table of enzyme activity measurement units\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Table_Conversion_of_Enzyme_Activity_Measurement_Units.pdf?4497787588428185691\" target=\"_blank\"\u003eConversion of Enzyme Activity Measurement Units\u003c\/a\u003e\u003c\/p\u003e","brand":"Marvelgent Biosciences","offers":[{"title":"100 μg","offer_id":16320281988,"sku":"14-SRCPB-100UG","price":251.0,"currency_code":"USD","in_stock":true},{"title":"5x 100 μg","offer_id":13383052689451,"sku":"14-SRCPB-500UG","price":918.0,"currency_code":"USD","in_stock":true},{"title":"1 mg","offer_id":16320282052,"sku":"14-SRCPB-1MG","price":1716.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/14-SRCPB_Carboxypeptidase_sequencing_grade.png?v=1493127272"},{"product_id":"proteosure-recombinant-endoproteinase-lys-c","title":"ProteoSure™ Recombinant Endoproteinase Lys-C, for Proteomic Sequencing and Mass Spectrometry Analysis","description":"\u003cp\u003eEndoproteinase Lys-C is a serine protease specific for cleavage of amide and peptide ester bonds at the carboxylic side of lysine and S-aminoethylcysteine residues.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eThe recombinant Endoproteinase Lys-C is genetically engineered from the \u003ci data-mce-fragment=\"1\"\u003eAchromobacter lyticus\u003c\/i\u003e lysyl endoproteinase. It is expressed in \u003cem data-mce-fragment=\"1\"\u003eE.coli\u003c\/em\u003e and purified by high pressure liquid chromatography (HPLC).  \u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eRecommended usage\u003c\/span\u003e\u003c\/h4\u003e\n\u003cp style=\"padding-left: 30px;\"\u003eThe recommended ratio to the sample protein is 1:1000 to 1:20000 (w\/w), the optimum pH is pH 7 - 9.5. The enzymatic reaction should be carried out at 30 - 37°C for 2 - 24 hours. \u003cbr\u003e\u003c\/p\u003e\n\u003cp style=\"padding-left: 30px;\"\u003eThe use of enzyme inhibitors (such as aprotinin, DFP, leupeptin, and TLCK) or protein denaturation with guanidine chloride (GuHCl) is not recommended. \u003c\/p\u003e\n\u003cp style=\"padding-left: 30px;\"\u003eRecombinant Lys-C is compatible with the following agents: 0.2% SDS, 1.0% Triton, 6 M Urea, 0.5 M NaCl, and 20% Acetonitrile.\u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eStorage and stability\u003c\/span\u003e\u003c\/h4\u003e\n\u003cp style=\"padding-left: 30px;\"\u003eStore Lys-C lyophilized powder in a sealed container at 2-8°C. It is stable for 12 months.\u003c\/p\u003e\n\u003cp style=\"padding-left: 30px;\"\u003eThe lyophilized powder can be reconstituted with ddH\u003csub\u003e2\u003c\/sub\u003eO to the working concentration; and the solution is stable at 2-8°C for 7 days.\u003c\/p\u003e\n\u003cp style=\"padding-left: 30px;\"\u003eIt is also recommended to dissolve the lyophilized powder in 25 mM Tris-HCl (pH 8.5) and make a stock concentrate solution. Aliquots of the stock solution may be stored at -20°C for up to 6 months. Avoid repeated freeze-thaw cycles.\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable height=\"297\" width=\"699\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"188\"\u003e\u003cstrong\u003eCAS #\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e78642-25-8\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eEnzyme Commission #\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e3.4.21.50\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMolecular weight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e31 kDa\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eOrigin\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003ci data-mce-fragment=\"1\"\u003eAchromobacter lyticus\u003c\/i\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSource\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cem\u003eE.coli\u003c\/em\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003ePurified by\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eHPLC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003ePhysical form\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003eLyophilized powder\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSpecific activity\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e≥ 3.0 AU\/mg protein\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"vertical-align: top;\"\u003e\u003cstrong\u003eUnit definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eOne Amidase Unit (AU) is the amount of enzyme that hydrolyzes 1 micromole Ac-Lys-pNA per minute at 25 mM Tris-HCl (pH 9.0) at 37°C.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eContaminant activity\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eNo trypsin, chymotrypsin, carboxypeptidase A, Lys-N, Arg-C or other protease contaminants.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStore in a sealed container at 2 - 8°C. Once reconstituted, it should be stored at -20°C for up to 6 months. Avoid freeze-and-thaw cycles.  \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eIntended use\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFor research only. Not intended for any human or animal diagnostic or therapeutic use.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ch5\u003eDocuments\u003c\/h5\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ch4\u003eTechnical Note\u003c\/h4\u003e\n\u003cp\u003e🗎    \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Table_Conversion_of_Enzyme_Activity_Measurement_Units.pdf?4497787588428185691\" title=\"Conversion table of enzyme activity measurement units\" target=\"_blank\"\u003eConversion of Enzyme Activity Measurement Units\u003c\/a\u003e\u003c\/p\u003e","brand":"Marvelgent Biosciences","offers":[{"title":"20 μg","offer_id":40631829332011,"sku":"14-LC-020UG","price":294.0,"currency_code":"USD","in_stock":true},{"title":"2x 20 μg","offer_id":39647651856427,"sku":"14-LC-040UG","price":530.0,"currency_code":"USD","in_stock":true},{"title":"100 μg","offer_id":39647641567275,"sku":"14-LC-100UG","price":1207.0,"currency_code":"USD","in_stock":true},{"title":"1 mg","offer_id":41218680913963,"sku":"14-LC-1MG","price":6872.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/14-SRCT_Chymotrypsin_sequencinggrade.png?v=1638373554"}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/collections\/Marvelgent_homepage_icon_-_proteases2.png?v=1781630474","url":"https:\/\/marvelgent.com\/collections\/protease\/application_mass-spectrometry-sequencing.oembed","provider":"Marvelgent Biosciences","version":"1.0","type":"link"}