{"title":"Protein Purification","description":"\u003cp\u003ePurification of the protein of interest is a fundamental step in studying individual protein. Various chromatographic and affinity techniques and tools have been developed and are readily adaptable for the downstream analysis. These methods separate the target protein based on its physical properties, including surface features, size and shape, net charge, as well as the availability of affinity group to a certain ligand.\u003c\/p\u003e\n\u003cp\u003eAt Marvelgent, we offer a wide range of chromatographic media and pre-packed cartridges for your various needs of purifying proteins with high yield, high purity. Explore our products from the following chromatograpy techniques:\u003c\/p\u003e\n\u003ctable style=\"border: solid 1px #ffffff;\" width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: solid 1px #ffffff; text-align: center; padding: 28px;\"\u003e\n\u003ca title=\"Group Specific Affinity Chromatography\" href=\"https:\/\/marvelgent.com\/collections\/group-specific-affinity-chromatography\"\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Protein_group_specific_affinity_chromatography_whitebackground200x200.png?11943334877776412148\" alt=\"Protein_group specific affinity chromatography\"\u003e\u003c\/a\u003e\u003cbr\u003e\n\u003ch3 style=\"text-align: center;\"\u003e\u003ca title=\"Group Specific Affinity Chromatography\" href=\"group-specific-affinity-chromatography\"\u003eGroup Specific Affinity Chromatography\u003c\/a\u003e\u003c\/h3\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border: solid 1px #ffffff; text-align: center; padding: 28px;\" width=\"23%\"\u003e\n\u003ca title=\"Immobilized-Metal Affinity Chromatography (IMAC)\" href=\"https:\/\/marvelgent.com\/collections\/immobilized-metal-affinity-chromatography\"\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Protein_immobilized_metal_affinity_chromatography_2_whitebackground_200x200.png?12167551023243969581\" alt=\"Protein_immobilized metal affinity chromatography\"\u003e\u003c\/a\u003e\u003cbr\u003e\n\u003ch3 style=\"text-align: center;\"\u003e\u003ca title=\"Immobilized-Metal Affinity Chromatography (IMAC)\" href=\"https:\/\/marvelgent.com\/collections\/immobilized-metal-affinity-chromatography\"\u003eImmobilized-Metal Affinity Chromatography\u003c\/a\u003e\u003c\/h3\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border: solid 1px #ffffff; text-align: center; padding: 28px;\" width=\"23%\"\u003e\n\u003ca title=\"Hydrophobic Interaction Chromatography HIC Media\" href=\"https:\/\/marvelgent.com\/collections\/hydrophobic-interaction-chromatography-media\"\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Protein_hydrophobic_interaction_chromatography_whitebackground_3_200x200.png?5796341248777821639\" alt=\"Protein_hydrophobic interaction chromatography\"\u003e\u003c\/a\u003e\u003cbr\u003e\n\u003ch3 style=\"text-align: center;\"\u003e\u003ca title=\"Hydrophobic Interaction Chromatography HIC Media\" href=\"https:\/\/marvelgent.com\/collections\/hydrophobic-interaction-chromatography-media\"\u003eHydrophobic Interaction Chromatography (HIC)\u003c\/a\u003e\u003c\/h3\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: solid 1px #ffffff; text-align: center; padding: 28px;\" width=\"23%\"\u003e\n\u003ca title=\"Ion exchange chromatography IEX media\" href=\"https:\/\/marvelgent.com\/collections\/ion-exchange-chromatography-iex\"\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Protein_IEX_chromatography_whitebackground_200x200.png?11890079813593855500\" alt=\"Protein_IEX chromatography\"\u003e\u003c\/a\u003e\u003cbr\u003e\n\u003ch3 style=\"text-align: center;\"\u003e\u003ca title=\"Ion exchange chromatography IEX media\" href=\"https:\/\/marvelgent.com\/collections\/ion-exchange-chromatography-iex\"\u003eIon Exchange Chromatography (IEX)\u003c\/a\u003e\u003c\/h3\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border: solid 1px #ffffff; text-align: center; padding: 28px;\" width=\"23%\"\u003e\n\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Protein_size_exclusion_chromatography_whitebackground_200x200_3a36a8b5-ca3a-48dc-a71a-21712b081f92.png?18390705250383726378\" alt=\"Protein_size exclusion chromatography\"\u003e\u003cbr\u003e\n\u003ch3 style=\"text-align: center;\"\u003eSize Exclusion Chromatography (SEC)\u003c\/h3\u003e\n\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #ffffff; text-align: center; padding: 28px;\" width=\"23%\"\u003e\n\u003ca title=\"Custom Chromatography Service\" href=\"https:\/\/marvelgent.com\/pages\/custom-chromatography-service\"\u003e\u003cimg style=\"display: block; float: none; margin-left: auto; margin-right: auto;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Protein_custom_chromatography_whitebackground_2_200x200.png?9054617128626519155\" alt=\"Protein_custom chromatography \u0026amp; activated media\"\u003e\u003c\/a\u003e\u003cbr\u003e\n\u003ch3 style=\"text-align: center;\"\u003e\u003ca title=\"Custom Chromatography Service\" href=\"https:\/\/marvelgent.com\/pages\/custom-chromatography-service\"\u003eCustom Chromatography \u0026amp; Activated Media\u003c\/a\u003e\u003c\/h3\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","products":[{"product_id":"proteindex-glutathione-agarose","title":"PROTEINDEX™ Glutathione Agarose","description":"\u003cp\u003e\u003cstrong\u003ePROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e\u003c\/strong\u003e™ Glutathione Agarose is an affinity chromatography medium designed for easy, one-step purification of recombinant glutathione S-transferase (GST) fusion proteins and other glutathione binding proteins expressed in \u003cem\u003eE. coli\u003c\/em\u003e, insect and mammalian cells.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eProduct Highlights\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003eHighly specific to the GST tag\u003c\/li\u003e\n\u003cli\u003eHigh ligand density for greater yield\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eApplications:\u003c\/span\u003e\u003c\/h4\u003e\n\u003cp\u003e\u003cstrong\u003ePurification of GST-tagged protein using ProteIndex™ Glutathione Agarose 4FF resins.\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"20%\"\u003e\u003c\/td\u003e\n\u003ctd width=\"20%\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/GST4FF.png?v=1500660148\" alt=\"\"\u003e\u003c\/td\u003e\n\u003ctd width=\"60%\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp style=\"padding-left: 60px;\"\u003eLanes: (1) MW; (2) Raw cell lysate; (3) Eluate.\u003c\/p\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"200\"\u003e\u003cstrong\u003eLigand\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eReduced glutathione \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eLigand concentration \u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e300 μmol Glutathione\/g medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBinding capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;20 mg GST (40 kDa)\/mL medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMatrix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e4% agarose supplied as 50% slurry\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eParticle size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e45 μm - 165 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaximum pressure\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.1 MPa (1 bar)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eRecommended flow rate\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003eGravity flow, \u0026lt;75 cm\/hour\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003epH stability\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003e3-12\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage buffer\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e20% ethanol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProduct is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e   \u003cimg alt=\"Keep refrigerated\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Keep-refrigerated_95fa1867-1f0d-47e6-823a-8a0008076e21_small.png?12332456762384261517\" style=\"float: none;\"\u003e   \u003cimg alt=\"Do not freeze\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Do-not-freeze_2_small.png?12332456762384261517\" style=\"float: none;\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003e\u003cbr\u003e\u003c\/h5\u003e\n\u003ch4 style=\"color: #ee8012;\"\u003e\u003cbr\u003e\u003c\/h4\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"10 mL","offer_id":14282545796,"sku":"11-0198-010","price":159.0,"currency_code":"USD","in_stock":true},{"title":"50 mL","offer_id":14282545860,"sku":"11-0198-050","price":797.0,"currency_code":"USD","in_stock":true},{"title":"100 mL","offer_id":14282545924,"sku":"11-0198-100","price":1588.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/11-0198_Glutathione_Agarose_bb71d204-df00-4196-9dab-caa0489ef2ab.png?v=1491950997"},{"product_id":"proteindex-glutathione-agarose-4-fast-flow","title":"PROTEINDEX™ Glutathione Agarose 4 Fast Flow","description":"\u003cp\u003e\u003cstrong\u003ePROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e\u003c\/strong\u003e™ Glutathione Agarose 4FF is an affinity chromatography medium developed for easy, one-step purification of recombinant glutathione S-transferase (GST) fusion proteins and other glutathione binding proteins expressed in \u003cem\u003eE. coli\u003c\/em\u003e, insect and mammalian cells. The rigid, highly cross-linked support is suitable for purification scale-up applications.\u003c\/p\u003e\n\u003cp\u003eOur proprietary ProteIndex™ coupling technology ensures stable immobilization of active ligand and minimal ligand leakage in a wide range of purification buffer conditions. The resins can be reused multiple times to purify the same target protein.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eProduct Highlights\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eHigh selectivity for high purity\u003c\/li\u003e\n\u003cli\u003e\u0026gt; 10 mg GST\/mL settled resins\u003c\/li\u003e\n\u003cli\u003eStable coupling of ligand, low leakage; can be re-used to purify the same protein\u003c\/li\u003e\n\u003cli\u003eVersatile, ready-to-use with convenient formats\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"10%\"\u003e\u003c\/td\u003e\n\u003ctd width=\"40%\"\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/GST_Beads_4FF2017050703_2.png?v=1508427878\"\u003e\u003c\/td\u003e\n\u003ctd valign=\"bottom\"\u003e\n\u003cp\u003e\u003cstrong\u003ePurification performance of Glutathione 4FF resins.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e50 mL of E.coli lysate containing a GST-tagged fusion protein was loaded on 0.5 mL packed Glutathione 4FF resins at 0.5 mL\/mL. The column was then washed with 10 mL of PBS. The target GST-tagged protein was eluted with 7.5 mL of elution buffer (50 mM Tris-HCl, 10 mM GSH, pH 8.0). Fractions of lysate, flow-through, and eluate were analysed by SDS-PAGE. Lanes: (1) Raw cell lysate; (2) Flow-through; (3) Eluate.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"200\"\u003e\u003cstrong\u003eLigand\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eGlutathione coupled with a 12-atom linker\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eLigand concentration \u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e120 - 320 μmol Glutathione\/mL medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBinding capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;10 mg GST (40 kDa)\/mL medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMatrix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e4% highly cross-linked agarose supplied as 50% slurry\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eParticle size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e45 μm - 165 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaximum pressure\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.3 MPa (3 bar, 43 psi)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eRecommended flow rate\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003e300 - 500 cm\/hour\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003epH stability\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003e3-12\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage buffer\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e20% ethanol as preservative\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProduct is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e   \u003cimg style=\"float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Keep-refrigerated_95fa1867-1f0d-47e6-823a-8a0008076e21_small.png?12332456762384261517\" alt=\"Keep refrigerated\"\u003e   \u003cimg style=\"float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Do-not-freeze_2_small.png?12332456762384261517\" alt=\"Do not freeze\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"10 mL","offer_id":14463117892,"sku":"11-0199-010","price":248.0,"currency_code":"USD","in_stock":true},{"title":"50 mL","offer_id":14463117956,"sku":"11-0199-050","price":935.0,"currency_code":"USD","in_stock":true},{"title":"100 mL","offer_id":14463118084,"sku":"11-0199-100","price":1716.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/11-0198_Glutathione_Agarose.png?v=1491948363"},{"product_id":"proteindex-glutathione-agarose-4-ff-prepacked-cartridge","title":"PROTEINDEX™ Glutathione Agarose 4 FF, Prepacked Cartridge","description":"\u003cp\u003e\u003cstrong\u003ePROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e\u003c\/strong\u003e™ Glutathione Agarose 4FF is an affinity chromatography medium developed for easy, one-step purification of recombinant glutathione S-transferase (GST) fusion proteins and other glutathione binding proteins expressed in \u003cem\u003eE. coli\u003c\/em\u003e, insect and mammalian cells. The rigid, highly cross-linked support is suitable for purification scale-up applications. The cartridge prepacked with Glutathione Agarose 4FF is ready-to-use, and is compatible with syringe, AKTA, and other peristaltic pump systems.\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eValue bundle with a \u003c\/span\u003e\u003ca href=\"https:\/\/marvelgent.com\/products\/connector-1-16-luer-female-10-32-male-peek\" title=\"Connector Luer Female \/ 10-32 Male in PEEK\" rel=\"noopener\" target=\"_blank\"\u003eLuer Female\/10-32 Male Connector in PEEK\u003c\/a\u003e\u003cspan\u003e (Cat# 20-0315) is now available for all pack sizes, providing convenient operation of the pre-packed column with a syringe at an excellent value.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"200\"\u003e\u003cstrong\u003eLigand\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eGlutathione coupled with a 12-atom linker\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eLigand concentration \u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e120 - 320 μmol Glutathione\/mL medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBinding capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;10 mg GST (26 kDa)\/mL medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMatrix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e4% highly cross-linked agarose\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eParticle size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e45 μm - 165 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaximum pressure\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.3 MPa (3 bar, 43 psi)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eRecommended flow rate\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003e300 - 500 cm\/hour\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003epH stability\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003e3-12\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage buffer\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e20% ethanol as preservative\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"5x 1 mL Columns","offer_id":14469007364,"sku":"11-0200-5x1ML","price":306.0,"currency_code":"USD","in_stock":true},{"title":"5x 1 mL Columns + 1 Connector","offer_id":41405722132523,"sku":"11-0200-5x1MLB","price":334.0,"currency_code":"USD","in_stock":true},{"title":"1x 5 mL Column","offer_id":14469007428,"sku":"11-0200-1x5ML","price":289.0,"currency_code":"USD","in_stock":true},{"title":"1x 5 mL Column + 1 Connector","offer_id":41405722198059,"sku":"11-0200-1x5MLB","price":316.0,"currency_code":"USD","in_stock":true},{"title":"5x 5 mL Columns","offer_id":14469007492,"sku":"11-0200-5x5ML","price":1207.0,"currency_code":"USD","in_stock":true},{"title":"5x 5 mL Columns + 1 Connector","offer_id":41405722165291,"sku":"11-0200-5x5MLB","price":1235.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/Marvelgent_pre-packed_cartridges_c684d440-3246-420b-adc9-fed0b96077d5.png?v=1493134302"},{"product_id":"proteindex-rprotein-a-agarose","title":"PROTEINDEX™ rProtein A Agarose, Settled Resin","description":"\u003cp\u003e\u003cstrong\u003ePROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e\u003c\/strong\u003e™ rProtein A Agarose 4 Fast Flow is an affinity chromatography medium designed for easy, one-step purification of immunoglobulins, notably IgG isotypes, as well as Fc region-containing fusion proteins.\u003c\/p\u003e\n\u003cp\u003eProtein A, a bacterial cell wall protein isolated from \u003cem\u003eStaphylococcus aureus\u003c\/em\u003e, binds to the Fc region of most immunoglobulins through interactions with the heavy chain. Native Protein A contains five high affinity IgG binding domains and many other domains with unknown functions.\u003c\/p\u003e\n\u003cp\u003eThe recombinant Protein A is a genetically engineered protein that contains only the five high affinity IgG binding domains. The non-essential domains were removed to minimize nonspecific binding. The recombinant Protein A is produced in \u003ci\u003eE. coli, \u003c\/i\u003eand it essentially binds to immunoglobulins with the same degree of affinity as the native Protein A. rProtein A is capable of binding with high affinity to the Fc region from IgG of several species including human and rabbit.\u003c\/p\u003e\n\u003cp\u003eEnabled by proprietary ProteIndex™ technology, immobilization of recombinant Protein A on 4% agarose resins was very stable and was optimized for high yield in laboratory preparations and scale-up applications.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eProduct Highlights\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eHigh capacity, \u0026gt; 40 mg human IgG \/ mL of settled resins\u003c\/li\u003e\n\u003cli\u003eHigh selectivity for high purity\u003c\/li\u003e\n\u003cli\u003eMinimal ligand leakage, can be reused\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"5%\"\u003e\u003c\/td\u003e\n\u003ctd width=\"35%\"\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/ProteinA4FF_purifying_mAscites_fluid_3_0d442212-1ce9-4a05-8106-1ec2848dd3cb.png?v=1508439312\"\u003e\u003c\/td\u003e\n\u003ctd valign=\"bottom\"\u003e\n\u003cp\u003e\u003cstrong\u003eHigh purity and high capacity of rProtein A 4FF resin. \u003c\/strong\u003e2 mL mouse ascites fluid was loaded on the column containing 0.5 mL settled rProtein A 4 FF resin. Bound immunoglobulin species were eluted with 100 mM Glycine, pH 3.0. Fractions were analyzed by SDS-PAGE.\u003c\/p\u003e\n\u003cp\u003eLanes: (1) Raw ascites fluid; (2) Flow-through; (3) Elution. Note that, heavy and light chains of IgG were mostly absent from the flow-through, indicating that most immunoglobulins were bound to the column.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"200\"\u003e\u003cstrong\u003eLigand\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eRecombinant protein A produced in \u003cem\u003eE.coli\u003c\/em\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBinding capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;40 mg human IgG\/mL medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMatrix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e4% agarose supplied as 50% slurry\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eParticle size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e45 μm - 165 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaximum pressure\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.1 MPa (1 bar)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eChemical stability\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eAll commonly used aqueous buffers, including 6 M guanidine-HCl and 8 M urea.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eRecommended flow rate\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eGravity flow, 100 - 300 cm\/hour\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003epH stability\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003eLong term: pH3 - 9;\u003c\/p\u003e\n\u003cp\u003eShort term: pH2 - 10\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage buffer\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e20% ethanol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProduct is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e   \u003cimg style=\"float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Keep-refrigerated_95fa1867-1f0d-47e6-823a-8a0008076e21_small.png?12332456762384261517\" alt=\"Keep refrigerated\"\u003e   \u003cimg style=\"float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Do-not-freeze_2_small.png?12332456762384261517\" alt=\"Do not freeze\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eDocuments\u003c\/h5\u003e\n\u003ch4\u003eTechnical Note\u003c\/h4\u003e\n\u003cp\u003e🗎    \u003ca rel=\"noopener noreferrer\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Table_Binding_characteristics_of_immunoglobulin-binding_proteins.pdf?6231220717744860621\" title=\"Comparison of binding characteristics of immunoglobulin-binding proteins\" target=\"_blank\"\u003eComparison of Binding Characteristics of Immunoglobulin-Binding Proteins\u003c\/a\u003e\u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"5 mL","offer_id":14587446980,"sku":"11-0202-005","price":159.0,"currency_code":"USD","in_stock":true},{"title":"25 mL","offer_id":14587447044,"sku":"11-0202-025","price":638.0,"currency_code":"USD","in_stock":true},{"title":"100 mL","offer_id":14587447108,"sku":"11-0202-100","price":1716.0,"currency_code":"USD","in_stock":true},{"title":"200 mL","offer_id":5934033633319,"sku":"11-0202-200","price":3190.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/11-0202_ProteinA.png?v=1508438549"},{"product_id":"proteindex-rprotein-a-agarose-4ff","title":"PROTEINDEX™ rProtein A Agarose 4 Fast Flow","description":"\u003cp\u003e\u003cstrong\u003ePROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e\u003c\/strong\u003e™ rProtein A Agarose 4 Fast Flow is an affinity chromatography medium designed for easy, one-step purification of immunoglobulins, notably IgG isotypes, as well as Fc region-containing fusion proteins.\u003c\/p\u003e\n\u003cp\u003eProtein A, a bacterial cell wall protein isolated from \u003cem\u003eStaphylococcus aureus\u003c\/em\u003e, binds to the Fc region of most immunoglobulins through interactions with the heavy chain. Native Protein A contains five high affinity IgG binding domains and many other domains with unknown functions.\u003c\/p\u003e\n\u003cp\u003eThe recombinant Protein A is a genetically engineered protein that contains only the five high affinity IgG binding domains. The non-essential domains were removed to minimize nonspecific binding. The recombinant Protein A is produced in \u003ci\u003eE. coli, \u003c\/i\u003eand it essentially binds to immunoglobulins with the same degree of affinity as the native Protein A. rProtein A is capable of binding with high affinity to the Fc region from IgG of several species including human and rabbit.\u003c\/p\u003e\n\u003cp\u003eEnabled by proprietary ProteIndex™ technology, immobilization of recombinant Protein A on 4% highly cross-linked agarose resins was very stable and was optimized for high yield in laboratory preparations and scale-up applications.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eProduct Highlights\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eHigh capacity, \u0026gt; 40 mg human IgG \/ mL of settled resins\u003c\/li\u003e\n\u003cli\u003eHigh selectivity for high purity\u003c\/li\u003e\n\u003cli\u003eMechanically stable matrix support, can be used in spin (at up to 1000 g) and automated applications\u003c\/li\u003e\n\u003cli\u003eMinimal ligand leakage, can be reused\u003c\/li\u003e\n\u003cli\u003eVersatile, ready-to-use with convenient formats\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"5%\"\u003e\u003c\/td\u003e\n\u003ctd width=\"35%\"\u003e \u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/ProteinA4FF_purifying_mAscites_fluid_3_0d442212-1ce9-4a05-8106-1ec2848dd3cb.png?v=1508439312\"\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"bottom\"\u003e\n\u003cp\u003e\u003cstrong\u003eHigh purity and high capacity of rProtein A 4FF resin. \u003c\/strong\u003e2 mL mouse ascites fluid was loaded on the column containing 0.5 mL settled rProtein A 4 FF resin. Bound immunoglobulin species were eluted with 100 mM Glycine, pH 3.0. Fractions were analyzed by SDS-PAGE.\u003c\/p\u003e\n\u003cp\u003eLanes: (1) Raw ascites fluid; (2) Flow-through; (3) Elution. Note that, heavy and light chains of IgG were mostly absent from the flow-through, indicating that most immunoglobulins were bound to the column.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"200\"\u003e\u003cstrong\u003eLigand\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eRecombinant protein A with Ig binding domains\u003cem\u003e\u003cbr\u003e\u003c\/em\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBinding capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;40 mg human IgG\/mL medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMatrix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eCross-linked 4% agarose supplied as a 50% slurry\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eParticle size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e45 μm - 165 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaximum pressure\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.3 MPa (3 bar)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eChemical stability\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eAll commonly used aqueous buffers, including 6 M guanidine-HCl and 8 M urea.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eRecommended flow rate\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eGravity flow and medium pressure, 30 - 300 cm\/hour\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003epH stability\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003eLong term: pH 2 - 9;\u003c\/p\u003e\n\u003cp\u003eShort term: pH 2 - 11\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage buffer\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e20% ethanol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProduct is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e   \u003cimg style=\"float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Keep-refrigerated_95fa1867-1f0d-47e6-823a-8a0008076e21_small.png?12332456762384261517\" alt=\"Keep refrigerated\"\u003e   \u003cimg style=\"float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Do-not-freeze_2_small.png?12332456762384261517\" alt=\"Do not freeze\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eDocuments\u003c\/h5\u003e\n\u003ch4\u003eTechnical Note\u003c\/h4\u003e\n\u003cp\u003e🗎    \u003ca rel=\"noopener noreferrer\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Table_Binding_characteristics_of_immunoglobulin-binding_proteins.pdf?6231220717744860621\" title=\"Comparison of binding characteristics of immunoglobulin-binding proteins\" target=\"_blank\"\u003eComparison of Binding Characteristics of Immunoglobulin-Binding Proteins\u003c\/a\u003e\u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"5 mL","offer_id":14675386436,"sku":"11-0203-005","price":379.0,"currency_code":"USD","in_stock":true},{"title":"25 mL","offer_id":14675386500,"sku":"11-0203-025","price":1461.0,"currency_code":"USD","in_stock":true},{"title":"100 mL","offer_id":5933537263655,"sku":"11-0203-100","price":3025.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/11-0203_ProteinA4FF.png?v=1513270183"},{"product_id":"proteindex-rprotein-a-agarose-4ff-prepacked-cartridge","title":"PROTEINDEX™ rProtein A Agarose 4FF, Prepacked Cartridge","description":"\u003cp\u003e\u003cstrong\u003ePROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e\u003c\/strong\u003e™ rProtein A Agarose 4 Fast Flow is an affinity chromatography medium designed for easy, one-step purification of immunoglobulins, notably IgG isotypes, as well as Fc region-containing fusion proteins.\u003c\/p\u003e\n\u003cp\u003eProtein A, a bacterial cell wall protein isolated from \u003cem\u003eStaphylococcus aureus\u003c\/em\u003e, binds to the Fc region of most immunoglobulins through interactions with the heavy chain. Native Protein A contains five high affinity IgG binding domains and many other domains with unknown functions. The recombinant Protein A coupled to the agarose resin is produced in \u003ci\u003eE. coli\u003c\/i\u003e and contains five high affinity IgG binding domains with other non-essential domains removed to reduce nonspecific binding. Essentially the same as the native Protein A, rProtein A is capable of binding with high affinity to the Fc region from IgG of several species including human and rabbit.\u003c\/p\u003e\n\u003cp\u003eEnabled by proprietary ProteIndex™ technology, immobilization of recombinant Protein A on 4% highly cross-linked agarose resins was very stable and was optimized for high yield in laboratory preparations and scale-up applications.\u003c\/p\u003e\n\u003cp\u003eThe Prepacked Cartridge has standard interface and can be adapted to most commonly used chromatography systems, such as ÄKTA systems.\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eValue bundle with a \u003c\/span\u003e\u003ca rel=\"noopener\" title=\"Connector Luer Female \/ 10-32 Male in PEEK\" href=\"https:\/\/marvelgent.com\/products\/connector-1-16-luer-female-10-32-male-peek\" target=\"_blank\"\u003eLuer Female\/10-32 Male Connector in PEEK\u003c\/a\u003e\u003cspan\u003e (Cat# 20-0315) is now available for all pack sizes, providing convenient operation of the pre-packed column with a syringe at an excellent value.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eProduct Highlights\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eHigh capacity, \u0026gt; 40 mg human IgG \/ mL of settled resins\u003c\/li\u003e\n\u003cli\u003eHigh selectivity for high purity\u003c\/li\u003e\n\u003cli\u003eMinimal ligand leakage, can be reused\u003c\/li\u003e\n\u003cli\u003eReady-to-use, compatible with standard automated systems\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"5%\"\u003e\u003c\/td\u003e\n\u003ctd width=\"35%\"\u003e \u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/ProteinA4FF_purifying_mAscites_fluid_3_0d442212-1ce9-4a05-8106-1ec2848dd3cb.png?v=1508439312\"\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"bottom\"\u003e\n\u003cp\u003e\u003cstrong\u003eHigh purity and high capacity of rProtein A 4FF resin. \u003c\/strong\u003e2 mL mouse ascites fluid was loaded on the column containing 0.5 mL settled rProtein A 4 FF resin. Bound immunoglobulin species were eluted with 100 mM Glycine, pH 3.0. Fractions were analyzed by SDS-PAGE.\u003c\/p\u003e\n\u003cp\u003eLanes: (1) Raw ascites fluid; (2) Flow-through; (3) Elution. Note that, heavy and light chains of IgG were mostly absent from the flow-through, indicating that most immunoglobulins were bound to the column.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"200\"\u003e\u003cstrong\u003eLigand\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eRecombinant protein A produced in \u003cem\u003eE.coli\u003c\/em\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBinding capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;40 mg human IgG\/mL medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMatrix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eHighly cross-linked 4% agarose\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eParticle size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e45 μm - 165 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaximum pressure\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.3 MPa (3 bar)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eChemical stability\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eAll commonly used aqueous buffers, including 6 M guanidine-HCl and 8 M urea.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eRecommended flow rate\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eGravity flow and medium pressure, 30 - 300 cm\/hour\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003epH stability\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003eLong term: pH 3 - 10;\u003c\/p\u003e\n\u003cp\u003eShort term: pH 2 - 11.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage buffer\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e20% ethanol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProduct is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e   \u003cimg style=\"float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Keep-refrigerated_95fa1867-1f0d-47e6-823a-8a0008076e21_small.png?12332456762384261517\" alt=\"Keep refrigerated\"\u003e   \u003cimg style=\"float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Do-not-freeze_2_small.png?12332456762384261517\" alt=\"Do not freeze\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eDocuments\u003c\/h5\u003e\n\u003ch4\u003eTechnical Note\u003c\/h4\u003e\n\u003cp\u003e🗎    \u003ca rel=\"noopener noreferrer\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Table_Binding_characteristics_of_immunoglobulin-binding_proteins.pdf?6231220717744860621\" title=\"Comparison of binding characteristics of immunoglobulin-binding proteins\" target=\"_blank\"\u003eComparison of Binding Characteristics of Immunoglobulin-Binding Proteins\u003c\/a\u003e\u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"5x 1 mL Columns","offer_id":14710543492,"sku":"11-0204-5x1ML","price":572.0,"currency_code":"USD","in_stock":true},{"title":"5x 1 mL Columns with 1 Connector","offer_id":41405719740459,"sku":"11-0204-5x1MLB","price":599.0,"currency_code":"USD","in_stock":true},{"title":"1x 5 mL Column","offer_id":14710543556,"sku":"11-0204-1x5ML","price":555.0,"currency_code":"USD","in_stock":true},{"title":"1x 5 mL Column with 1 Connector","offer_id":41405719773227,"sku":"11-0204-1x5MLB","price":583.0,"currency_code":"USD","in_stock":true},{"title":"5x 5 mL Columns","offer_id":14710543620,"sku":"11-0204-5x5ML","price":2348.0,"currency_code":"USD","in_stock":true},{"title":"5x 5 mL Columns with 1 Connector","offer_id":41405719805995,"sku":"11-0204-5x5MLB","price":2376.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/Marvelgent_pre-packed_cartridges.png?v=1459891864"},{"product_id":"proteindex-rprotein-g-agarose","title":"PROTEINDEX™ rProtein G Agarose","description":"\u003cp\u003e\u003cstrong\u003ePROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e\u003c\/strong\u003e™ rProtein G Agarose is an affinity chromatography medium designed for easy, one-step purification of immunoglobulins, notably most mammallian IgG isotypes, as well as Fc region-containing fusion proteins.\u003c\/p\u003e\n\u003cp\u003eProtein G is derived from \u003cem\u003eStreptococcus\u003c\/em\u003e strain G148. It contains binding sites for the Fc portion of mammalian IgG. Native protein G has 3 IgG binding domains as well as the sites for albumin and cell-surface binding. Our recombinant protein G contains only the IgG binding domains but no albumin recognition region, therefore, it is compatible with purification protocols that require the presence of fetal bovine serum (FBS) or serum albumin proteins.\u003c\/p\u003e\n\u003cp\u003eShowing super binding specificity for IgG species, \u003cstrong\u003ePROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e\u003c\/strong\u003e™ rProtein G Agarose enables single-step purification of monoclonal and polyclonal antibodies with a greater than 95% purity, and is well suited for IgG purification from various sources, including crude samples of tissue cultures and sera.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eProduct Highlights\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eHigh capacity, \u0026gt; 30 mg human IgG \/ mL of settled resins\u003c\/li\u003e\n\u003cli\u003eHigh selectivity for high purity\u003c\/li\u003e\n\u003cli\u003eMinimal ligand leakage, can be reused\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"5%\"\u003e\u003c\/td\u003e\n\u003ctd width=\"45%\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/ProteinG_4FF_purifying_mAscites_fluid_2.png?v=1508441741\" alt=\"\"\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eHigh purity and high capacity of rProtein G 4FF resin. \u003c\/strong\u003e\u003cbr\u003e2 mL mouse ascites fluid was loaded on the column containing 0.5 mL settled rProtein G 4 FF resin. Bound immunoglobulin species were eluted with 100 mM Glycine, pH 3.0. Fractions were analyzed by SDS-PAGE.\u003c\/p\u003e\n\u003cp\u003eLanes: (1) Raw ascites fluid; (2) Flow-through; (3) Elution 1; (4) Elution 2. Note that, heavy and light chains of IgG were mostly absent from the flow-through, indicating that most immunoglobulins were bound to the column.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"200\"\u003e\u003cstrong\u003eLigand\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eRecombinant protein G produced in \u003cem\u003eE.coli\u003c\/em\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBinding capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;30 mg human IgG\/mL medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMatrix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e4% agarose supplied as 50% slurry\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eParticle size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e45 μm - 165 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaximum pressure\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.1 MPa (1 bar)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eChemical stability\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eAll commonly used aqueous buffers, including 6 M guanidine-HCl and 8 M urea.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eRecommended flow rate\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eGravity flow, 100 - 300 cm\/hour\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003epH stability\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003eLong term: pH3 - 9;\u003c\/p\u003e\n\u003cp\u003eShort term: pH2 - 10\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage buffer\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1x PBS containing 20% ethanol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProduct is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e   \u003cimg alt=\"Keep refrigerated\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Keep-refrigerated_95fa1867-1f0d-47e6-823a-8a0008076e21_small.png?12332456762384261517\" style=\"float: none;\"\u003e   \u003cimg alt=\"Do not freeze\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Do-not-freeze_2_small.png?12332456762384261517\" style=\"float: none;\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eDocuments\u003c\/h5\u003e\n\u003ch4\u003eTechnical Note\u003c\/h4\u003e\n\u003cp\u003e🗎    \u003ca title=\"Comparison of binding characteristics of immunoglobulin-binding proteins\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Table_Binding_characteristics_of_immunoglobulin-binding_proteins.pdf?6231220717744860621\" rel=\"noopener noreferrer\" target=\"_blank\"\u003eComparison of Binding Characteristics of Immunoglobulin-Binding Proteins\u003c\/a\u003e\u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"5 mL","offer_id":14711244548,"sku":"11-0205-005","price":159.0,"currency_code":"USD","in_stock":true},{"title":"25 mL","offer_id":14711244612,"sku":"11-0205-025","price":638.0,"currency_code":"USD","in_stock":true},{"title":"100 mL","offer_id":14711244676,"sku":"11-0205-100","price":1716.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/11-0205_ProteinG_Agarose.png?v=1491951858"},{"product_id":"proteindex-rprotein-g-agarose-4ff","title":"PROTEINDEX™ rProtein G Agarose 4 Fast Flow","description":"\u003cp\u003e\u003cstrong\u003ePROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e\u003c\/strong\u003e™ rProtein G Agarose 4FF is an affinity chromatography medium designed for easy, one-step purification of immunoglobulins, notably most mammallian IgG isotypes, as well as Fc region-containing fusion proteins.\u003c\/p\u003e\n\u003cp\u003eProtein G is derived from \u003cem\u003eStreptococcus\u003c\/em\u003e strain G148. It contains binding sites for the Fc portion of mammalian IgG. Native protein G has 3 IgG binding domains as well as the sites for albumin and cell-surface binding. Our recombinant protein G contains only the IgG binding domains but no albumin recognition region, therefore, it is compatible with purification protocols that require the presence of fetal bovine serum (FBS) or serum albumin proteins.\u003c\/p\u003e\n\u003cp\u003eShowing super binding specificity for IgG species, \u003cstrong\u003ePROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e\u003c\/strong\u003e™ rProtein G Agarose 4FF enables single-step purification of monoclonal and polyclonal antibodies with a greater than 95% purity, and is well suited for IgG purification from various sources, including crude samples of tissue cultures and sera. With rigid, highly cross-linked matrix support, this product is versatile and well chemical-tolerant. It is readily adaptable to protocols of various formats over a broad range of chemical conditions.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eProduct Highlights\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eHigh capacity, \u0026gt; 30 mg human IgG \/ mL of settled resins\u003c\/li\u003e\n\u003cli\u003eHigh selectivity for high purity\u003c\/li\u003e\n\u003cli\u003eMechanically stable matrix support, can be used in spin (at up to 1000 g) and automated applications\u003c\/li\u003e\n\u003cli\u003eMinimal ligand leakage, can be reused\u003c\/li\u003e\n\u003cli\u003eVersatile, ready-to-use with convenient formats\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"5%\"\u003e\u003c\/td\u003e\n\u003ctd width=\"45%\"\u003e \u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/ProteinG_4FF_purifying_mAscites_fluid_2.png?v=1508441741\"\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eHigh purity and high capacity of rProtein G 4FF resin. \u003c\/strong\u003e\u003cbr\u003e2 mL mouse ascites fluid was loaded on the column containing 0.5 mL settled rProtein G 4 FF resin. Bound immunoglobulin species were eluted with 100 mM Glycine, pH 3.0. Fractions were analyzed by SDS-PAGE.\u003c\/p\u003e\n\u003cp\u003eLanes: (1) Raw ascites fluid; (2) Flow-through; (3) Elution 1; (4) Elution 2. Note that, heavy and light chains of IgG were mostly absent from the flow-through, indicating that most immunoglobulins were bound to the column.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"200\"\u003e\u003cstrong\u003eLigand\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eRecombinant protein G produced in \u003cem\u003eE.coli\u003c\/em\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBinding capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;30 mg human IgG\/mL medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMatrix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eHighly cross-linked 4% agarose supplied as 50% slurry\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eParticle size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e45 μm - 165 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaximum pressure\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.3 MPa (3 bar)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eChemical stability\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eAll commonly used aqueous buffers, including 6 M guanidine-HCl and 8 M urea.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eRecommended flow rate\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eGravity flow, 100 - 300 cm\/hour\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003epH stability\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003eLong term: pH3 - 9;\u003c\/p\u003e\n\u003cp\u003eShort term: pH2 - 10\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage buffer\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1x PBS containing 20% ethanol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProduct is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e   \u003cimg style=\"float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Keep-refrigerated_95fa1867-1f0d-47e6-823a-8a0008076e21_small.png?12332456762384261517\" alt=\"Keep refrigerated\"\u003e   \u003cimg style=\"float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Do-not-freeze_2_small.png?12332456762384261517\" alt=\"Do not freeze\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eDocuments\u003c\/h5\u003e\n\u003ch4\u003eTechnical Note\u003c\/h4\u003e\n\u003cp\u003e🗎    \u003ca rel=\"noopener noreferrer\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Table_Binding_characteristics_of_immunoglobulin-binding_proteins.pdf?6231220717744860621\" title=\"Comparison of binding characteristics of immunoglobulin-binding proteins\" target=\"_blank\"\u003eComparison of Binding Characteristics of Immunoglobulin-Binding Proteins\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"5 mL","offer_id":14872941252,"sku":"11-0206-005","price":500.0,"currency_code":"USD","in_stock":true},{"title":"25 mL","offer_id":14872941316,"sku":"11-0206-025","price":1842.0,"currency_code":"USD","in_stock":true},{"title":"100 mL","offer_id":13526966468651,"sku":"11-0206-100","price":3173.0,"currency_code":"USD","in_stock":true},{"title":"200 mL","offer_id":14872941380,"sku":"11-0206-200","price":4895.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/11-0205_ProteinG_Agarose_122d73f6-280c-4aa5-b484-b5cfd64a32fe.png?v=1493138855"},{"product_id":"proteindex-rprotein-g-agarose-4-ff-br-prepacked-cartridge","title":"PROTEINDEX™ rProtein G Agarose 4 FF, Prepacked Cartridge","description":"\u003cp\u003e\u003cstrong\u003ePROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e\u003c\/strong\u003e™ rProtein G Agarose 4FF is an affinity chromatography medium designed for easy, one-step purification of immunoglobulins, notably most mammallian IgG isotypes, as well as Fc region-containing fusion proteins.\u003c\/p\u003e\n\u003cp\u003eProtein G is derived from \u003cem\u003eStreptococcus\u003c\/em\u003e strain G148. It contains binding sites for the Fc portion of mammalian IgG. Native protein G has 3 IgG binding domains as well as the sites for albumin and cell-surface binding. Our recombinant protein G contains only the IgG binding domains but no albumin recognition region, therefore, it is compatible with purification protocols that require the presence of fetal bovine serum (FBS) or serum albumin proteins.\u003c\/p\u003e\n\u003cp\u003eShowing super binding specificity for IgG species, \u003cstrong\u003ePROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e\u003c\/strong\u003e™ rProtein G Agarose 4FF enables single-step purification of monoclonal and polyclonal antibodies with a greater than 95% purity, and is well suited for IgG purification from various sources, including crude samples of tissue cultures and sera. With rigid, highly cross-linked matrix support, this product is versatile and well chemical-tolerant. It is readily adaptable to protocols of various formats over a broad range of chemical conditions.\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eValue bundle with a \u003c\/span\u003e\u003ca rel=\"noopener\" title=\"Connector Luer Female \/ 10-32 Male in PEEK\" href=\"https:\/\/marvelgent.com\/products\/connector-1-16-luer-female-10-32-male-peek\" target=\"_blank\"\u003eLuer Female\/10-32 Male Connector in PEEK\u003c\/a\u003e\u003cspan\u003e (Cat# 20-0315) is now available for all pack sizes, providing convenient operation of the pre-packed column with a syringe at an excellent value.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"200\"\u003e\u003cstrong\u003eLigand\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eRecombinant protein G produced in \u003cem\u003eE.coli\u003c\/em\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBinding capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;30 mg human IgG\/mL medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMatrix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eHighly cross-linked 4% agarose\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eParticle size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e45 μm - 165 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaximum pressure\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.3 MPa (3 bar)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eChemical stability\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eAll commonly used aqueous buffers, including 6 M guanidine-HCl and 8 M urea.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eRecommended flow rate\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eGravity flow, 100 - 300 cm\/hour\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003epH stability\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003eLong term: pH3 - 9;\u003c\/p\u003e\n\u003cp\u003eShort term: pH2 - 10\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage buffer\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1x PBS containing 20% ethanol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProduct is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e   \u003cimg style=\"float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Keep-refrigerated_95fa1867-1f0d-47e6-823a-8a0008076e21_small.png?12332456762384261517\" alt=\"Keep refrigerated\"\u003e   \u003cimg style=\"float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Do-not-freeze_2_small.png?12332456762384261517\" alt=\"Do not freeze\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eDocuments\u003c\/h5\u003e\n\u003ch4\u003eTechnical Note\u003c\/h4\u003e\n\u003cp\u003e🗎    \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Table_Binding_characteristics_of_immunoglobulin-binding_proteins.pdf?6231220717744860621\" title=\"Comparison of binding characteristics of immunoglobulin-binding proteins\" target=\"_blank\"\u003eComparison of Binding Characteristics of Immunoglobulin-Binding Proteins\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"1 mL Columns, Pack of 5","offer_id":14876203012,"sku":"11-0209-5x1ML","price":643.0,"currency_code":"USD","in_stock":true},{"title":"1 mL Columns, Pack of 5 with 1 Connector","offer_id":41405714661419,"sku":"11-0209-5x1MLB","price":671.0,"currency_code":"USD","in_stock":true},{"title":"5 mL Column, Pack of 1","offer_id":14876203076,"sku":"11-0209-1x5ML","price":610.0,"currency_code":"USD","in_stock":true},{"title":"5 mL Column, Pack of 1 with 1 Connector","offer_id":41405715906603,"sku":"11-0209-1x5MLB","price":638.0,"currency_code":"USD","in_stock":true},{"title":"5 mL Columns, Pack of 5","offer_id":14876203140,"sku":"11-0209-5x5ML","price":2637.0,"currency_code":"USD","in_stock":true},{"title":"5 mL Columns, Pack of 5 with 1 Connector","offer_id":41405716037675,"sku":"11-0209-5x5MLB","price":2665.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/Marvelgent_pre-packed_cartridges_0aa3b5cc-ca63-442e-8a8c-e8f8548ca6ff.png?v=1459891896"},{"product_id":"proteindex-rprotein-a-g-agarose-4-fast-flow","title":"PROTEINDEX™ rProtein A\/G Agarose 4 Fast Flow","description":"\u003cp\u003e\u003cstrong\u003ePROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e\u003c\/strong\u003e™ rProtein A\/G Agarose 4FF is an affinity chromatography medium designed for easy, one-step purification of a boarder range of immunoglobulin classes and subclasses. It is versatile, adaptable tool for antibody purification and immunoprecipitation.\u003c\/p\u003e\n\u003cp\u003eRecombinant fusion Protein A\/G is a genetically engineered protein (MW ~ 50 kDa) that contains four Fc binding domains from Protein A and two from Protein G and therefore combines the IgG binding profiles of both Protein A and Protein G. The binding of rProtein A\/G to immunoglobulins shows less pH-dependent than Protein A. In addition, unlike native Protein A and Protein G that contain albumin-recognition region and binding sites for other proteins, rProtein A\/G has these non-immunoglobulin binding sites eliminated, showing super binding specificity for a variety of immunoglobulins.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003ePROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX \u003c\/span\u003e\u003c\/strong\u003erProtein A\/G Agarose 4FF has rProtein A\/G immobolized onto highly cross-linked 4% agarose beads via a stable thioether linkage. The coupling technique is optimized to give a high binding capacity for IgG.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eProduct Highlights\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cspan style=\"color: #262626; font-family: Arial;\"\u003e10 ~15 mg hIgG\/mL resins\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan style=\"color: #262626; font-family: Arial;\"\u003eOptimized for immunoprecipitation\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan style=\"color: #262626; font-family: Arial;\"\u003eBinds to a broader range of Ig species\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003eMechanically stable matrix support, can be used in spin (at up to 1000 g) and automated applications\u003c\/li\u003e\n\u003cli\u003eMinimal ligand leakage, can be reused\u003c\/li\u003e\n\u003cli\u003eVersatile, ready-to-use with convenient formats\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"5%\"\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\" width=\"35%\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/ProteinA-G_Magpoly_2.png?v=1509412409\" alt=\"\"\u003e\u003c\/td\u003e\n\u003ctd valign=\"bottom\"\u003e\n\u003cp\u003e\u003cstrong\u003ePurification of rabbit IgG using rProtein A\/G 4FF resin. \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eLanes: (1) Raw rabbit serum; (2) Flow-through; (3) Elution.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"200\"\u003e\u003cstrong\u003eLigand\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eRecombinant fusion protein A\/G produced in E.coli\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBinding capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e10 ~ 15 mg human IgG\/mL medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMatrix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eHighly cross-linked 4% agarose supplied as 50% slurry\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eParticle size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e45 μm - 165 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaximum pressure\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.3 MPa (3 bar)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eChemical stability\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eAll commonly used aqueous buffers, including 6 M guanidine-HCl and 8 M urea.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eRecommended flow rate\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eGravity flow, 100 - 300 cm\/hour\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003epH stability\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003epH3 - 10\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1x PBS containing 20% ethanol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProduct is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e   \u003cimg alt=\"Keep refrigerated\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Keep-refrigerated_95fa1867-1f0d-47e6-823a-8a0008076e21_small.png?12332456762384261517\" style=\"float: none;\"\u003e   \u003cimg alt=\"Do not freeze\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Do-not-freeze_2_small.png?12332456762384261517\" style=\"float: none;\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eDocuments\u003c\/h5\u003e\n\u003ch4 style=\"color: #ee8012;\"\u003eTechnical Note\u003c\/h4\u003e\n\u003cp\u003e🗎    \u003ca title=\"Comparison of binding characteristics of immunoglobulin-binding proteins\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Table_Binding_characteristics_of_immunoglobulin-binding_proteins.pdf?6231220717744860621\" rel=\"noopener noreferrer\" target=\"_blank\"\u003eComparison of Binding Characteristics of Immunoglobulin-Binding Proteins\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"5 mL","offer_id":14880312132,"sku":"11-0210-005","price":253.0,"currency_code":"USD","in_stock":true},{"title":"25 mL","offer_id":14880312196,"sku":"11-0210-025","price":962.0,"currency_code":"USD","in_stock":true},{"title":"100 mL","offer_id":14880312260,"sku":"11-0210-100","price":2750.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/11-0210_ProteinAG-4FF.png?v=1491951604"},{"product_id":"proteindex-rprotein-a-alkali-tolerant-agarose-4ff","title":"PROTEINDEX™ Alkali-Tolerant Protein A Agarose 4 Fast Flow","description":"\u003cp\u003e\u003cstrong\u003ePROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e\u003c\/strong\u003e™ rProtein A Agarose 4 Fast Flow is a proprietary affinity chromatography medium designed for easy, one-step purification of immunoglobulins, notably monoclonal antibodies, from samples of larger volumes.\u003c\/p\u003e\n\u003cp\u003eAlkali-tolerant protein A is engineered by gene recombination technology on the base of native protein A. Our Alkali-Tolerant Protein A Agarose 4FF retains ≥95% capacity after 200 times repeated clean-in-place (CIP) cycles with 0.1M NaOH, and approximately 80% of the initial capacity with 0.5M NaOH after 100 times repeated CIP cycles. Alkali-Tolerant Protein A is immobilized onto the cross-linked 4% agarose resin with an orientation that is optimal for immunoglobulin binding. The level of leakage of the ligand during elution is very low.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"200\"\u003e\u003cstrong\u003eLigand\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eRecombinant alkali-tolerant protein A produced in \u003cem\u003eE.coli\u003c\/em\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBinding capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;40 mg rabbit IgG\/mL medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMatrix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eCross-linked 4% agarose supplied as a 50% slurry\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eParticle size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e45 μm - 165 μm, with an average of ~ 90 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaximum pressure\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.3 MPa (3 bar)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eRecommended flow rate\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eGravity flow and medium pressure, 30 - 300 cm\/hour\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eChemical stability\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eAll commonly used aqueous buffers and reagents in the process of antibody purification, including 6 M guanidine-HCl and 8 M urea.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eCleaning-in-place (CIP)\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.1 M ~ 0.5 M NaOH\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage buffer\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e20% ethanol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProduct is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e   \u003cimg alt=\"Keep refrigerated\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Keep-refrigerated_95fa1867-1f0d-47e6-823a-8a0008076e21_small.png?12332456762384261517\" style=\"float: none;\"\u003e   \u003cimg alt=\"Do not freeze\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Do-not-freeze_2_small.png?12332456762384261517\" style=\"float: none;\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eDocuments\u003c\/h5\u003e\n\u003ch4\u003eTechnical Note\u003c\/h4\u003e\n\u003cp\u003e🗎    \u003ca title=\"Comparison of binding characteristics of immunoglobulin-binding proteins\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Table_Binding_characteristics_of_immunoglobulin-binding_proteins.pdf?6231220717744860621\" target=\"_blank\"\u003eComparison of Binding Characteristics of Immunoglobulin-Binding Proteins\u003c\/a\u003e\u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"10 mL","offer_id":16984774020,"sku":"11-0211-010","price":1207.0,"currency_code":"USD","in_stock":true},{"title":"50 mL","offer_id":16984774084,"sku":"11-0211-050","price":3638.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/Protein_At_agarose4FF.png?v=1490749970"},{"product_id":"proteindex-protein-a-alkali-tolerant-agarose-4ff-prepacked-cartridge","title":"PROTEINDEX™ Alkali-Tolerant Protein A Agarose 4FF, Prepacked Cartridge","description":"\u003cp\u003e\u003cstrong\u003ePROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e\u003c\/strong\u003e™ rProtein A Agarose 4 Fast Flow is a proprietary affinity chromatography medium designed for easy, one-step purification of immunoglobulins, notably monoclonal antibodies, from samples of larger volumes.\u003c\/p\u003e\n\u003cp\u003eAlkali-tolerant protein A is engineered by gene recombination technology on the base of native protein A. Our Alkali-Tolerant Protein A Agarose 4FF retains ≥95% capacity after 200 times repeated clean-in-place (CIP) cycles with 0.1M NaOH, and approximately 80% of the initial capacity with 0.5M NaOH after 100 times repeated CIP cycles. Alkali-Tolerant Protein A is immobilized onto the cross-linked 4% agarose resin with an orientation that is optimal for immunoglobulin binding. The level of leakage of the ligand during elution is very low.\u003c\/p\u003e\n\u003cp\u003eThe Prepacked Cartridge has standard interface and can be adapted to most commonly used chromatography systems, such as ÄKTA systems.\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eValue bundle with a \u003c\/span\u003e\u003ca href=\"https:\/\/marvelgent.com\/products\/connector-1-16-luer-female-10-32-male-peek\" title=\"Connector Luer Female \/ 10-32 Male in PEEK\" rel=\"noopener\" target=\"_blank\"\u003eLuer Female\/10-32 Male Connector in PEEK\u003c\/a\u003e\u003cspan\u003e (Cat# 20-0315) is now available for all pack sizes, providing convenient operation of the pre-packed column with a syringe at an excellent value.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"200\"\u003e\u003cstrong\u003eLigand\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eRecombinant alkali-tolerant protein A produced in \u003cem\u003eE.coli\u003c\/em\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBinding capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;40 mg rabbit IgG\/mL medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMatrix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eCross-linked 4% agarose\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eParticle size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e45 μm - 165 μm, with an average size of ~ 90 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaximum pressure\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.3 MPa (3 bar)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eRecommended flow rate\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eGravity flow and medium pressure, 30 - 300 cm\/hour\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eChemical stability\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eAll commonly used aqueous buffers and reagents in the process of antibody purification, including 6 M guanidine-HCl and 8 M urea.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eCleaning-in-place (CIP)\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.1 M ~ 0.5 M NaOH\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage buffer\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e20% ethanol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProduct is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e   \u003cimg style=\"float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Keep-refrigerated_95fa1867-1f0d-47e6-823a-8a0008076e21_small.png?12332456762384261517\" alt=\"Keep refrigerated\"\u003e   \u003cimg style=\"float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Do-not-freeze_2_small.png?12332456762384261517\" alt=\"Do not freeze\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eDocuments\u003c\/h5\u003e\n\u003ch4\u003eTechnical Note\u003c\/h4\u003e\n\u003cp\u003e🗎    \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Table_Binding_characteristics_of_immunoglobulin-binding_proteins.pdf?6231220717744860621\" title=\"Comparison of binding characteristics of immunoglobulin-binding proteins\" target=\"_blank\"\u003eComparison of Binding Characteristics of Immunoglobulin-Binding Proteins\u003c\/a\u003e\u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"5x 1 mL Columns","offer_id":16990807748,"sku":"11-0212-5x1ML","price":643.0,"currency_code":"USD","in_stock":true},{"title":"5x 1 mL Columns with 1 Connector","offer_id":41405722329131,"sku":"11-0212-5x1MLB","price":671.0,"currency_code":"USD","in_stock":true},{"title":"1x 5 mL Column","offer_id":16990807812,"sku":"11-0212-1x5ML","price":610.0,"currency_code":"USD","in_stock":true},{"title":"1x 5 mL Column with 1 Connector","offer_id":41405722361899,"sku":"11-0212-1x5MLB","price":638.0,"currency_code":"USD","in_stock":true},{"title":"5x 5 mL Columns","offer_id":16990807876,"sku":"11-0212-5x5ML","price":2637.0,"currency_code":"USD","in_stock":true},{"title":"5x 5 mL Columns with 1 Connector","offer_id":41405722394667,"sku":"11-0212-5x5MLB","price":2665.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/ProteinAt_column2.png?v=1493134475"},{"product_id":"proteindex-ni-penta-agarose-chemical-tolerant","title":"PROTEINDEX™ Ni-Penta™ Agarose, Chemical Stable","description":"\u003cp\u003e\u003cstrong\u003ePROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e\u003c\/strong\u003e™ \u003cstrong\u003eChemical-Tolerant Ni-Penta™ Agarose\u003c\/strong\u003e is a new immobilized metal ion affinity chromatography (IMAC) medium precharged with nickel ions, designed mainly for purification of low abundant proteins or challenging proteins in conditions where EDTA, DTT, β-ME, and other stabilizing agents are necessary for maintaining protein stability and integrity.\u003c\/p\u003e\n\u003cp\u003eThe nickel ion binds very strongly to the matrix support in complex with a proprietary pentadentate chelating ligand, which immobilizes the nickel ion through 5 sites to form a homogeneous,  chemical-stable structure. This characteristic nickel ion bonding on the Ni-Penta™ resin provides excellent resistance to EDTA, NaOH, β-ME, and other reagents that are restricted in the purification protocol using conventional NTA- and IDA- functionalized resins.\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"http:\/\/marvelgent.com\/pages\/high-selectivity-ni-penta-agarose-resins-for-secreted-proteins\" title=\"Ni-Penta resins for high selective capture of polyHis-tagged proteins\"\u003eRead more on Ni-Penta properties contributing to high selective purification of His-tagged proteins ...\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eProduct Highlights\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eEDTA-resistant\u003c\/li\u003e\n\u003cli\u003eHigh selectivity for high purity\u003c\/li\u003e\n\u003cli\u003eRetained high 6xHis affinity in various buffers\u003c\/li\u003e\n\u003cli\u003eWell suited for direct capture of secreted polyHis-tagged proteins from eukaryotic cell culture supernatant\u003c\/li\u003e\n\u003cli\u003eRecharge-free with minimal nickel ion leakage\u003c\/li\u003e\n\u003cli\u003eRequires less imidazole\u003c\/li\u003e\n\u003cli\u003eNaOH-tolerant\u003c\/li\u003e\n\u003cli\u003eVersatile, ready-to-use with convenient formats\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eApplications:\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cstrong\u003ePurification of secreted His-tagged proteins directly from eukaryotic cell culture supernatants\u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003eOwing to its proprietary ligand for nickel ion immobilization, Ni-Penta™ resin tolerates EDTA very well, and its affinity for His-tag can be retained in presence of EDTA up to 10 mM. To purify secreted proteins that are produced from eukaryotic cell cultures (such as insect High Five™ cells* and Sf9 cells, CHO cells, 293 cells etc.), a step of removing incompatible agents to Ni-NTA resins by dialysis is frequently required prior to loading the material on the column. This is tedious, time-consuming, and labor intensive. By using Ni-Penta resins, sample pretreament can be skipped, and the culture medium can be loaded directly to the column for purification.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cstrong\u003ePurification of His-tagged proteins that require presence of EDTA, DTT, β-ME, and other agents for maximum protein stability and integrity\u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003ePROTEINDEX™ Ni-Penta™ Agarose Resin is compatible with a wide range of buffers including chelating agents (e.g. EDTA) and reducing agents (e.g. DTT), which are incompatible and therefore should be avoid in purification applications using conventional NTA or IDA resins. Ni-Penta™ resin helps to simplify your sample preparation process and allows your target proteins be purified at their preferred conditions.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cstrong\u003eRepetitive uses of the column that require clean-in-place with NaOH but no nickel recharge \u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003eAnti-strip power of the Ni-Penta™ enables repetitive uses of the column without the need to recharge nickel ion, even after multiple cycles of CIP with 1 M NaOH.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cstrong\u003eRestricted imidazole amount in the elution buffer due to the requirements in downstream applications or the target protein's sensitivity to imidazole\u003cbr\u003e\u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003eImidazole is general required to minimize non-specific binding on Ni-NTA resins. Ni-Penta™ Agarose Resin is highly selective for His-tag and therefore does not demand imidazole for minimizing non-specific binding throughout purification. No imidazole is required in the binding buffer, and 40 mM to 100 mM of imidazole can efficiently elute most target proteins.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003e Technical Notes\u003c\/span\u003e\u003c\/h4\u003e\n\u003ch4\u003e 1. EDTA-stable Ni-Penta chemistry for minimal nickel ion leakage\u003c\/h4\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Ni-Penta_8_99b871bb-a5a2-4123-8891-2437bac436d6.png?v=1493146106\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eFigure 1. Strong chelate chemistry for immobilization of nickel ion. \u003c\/b\u003eNi-Penta Agarose 6 FF resins were incubated at room temperature for 2 hours in 5 volumes of a serial dilutions of EDTA from 10 mM to 400 mM. The amount of nickel ions released into the buffer was measured.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eResult:\u003c\/strong\u003e During 2 hours of incubation in 50 mM EDTA, less than 5 % of nickel ions leaked from Ni-Penta resins. In comparison, 50 mM EDTA is sufficient to strip off nickel ions from conventional Ni-NTA resins and has been used routinely to regenerate Ni-NTA resin.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e2. Uncompromised His-tag protein binding in EDTA up to 10 mM\u003c\/h4\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"color: #3d85c6;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Ni-Penta_7_ad68e7f9-16be-43e9-a619-dcfe21d2590f.png?v=1493145893\" alt=\"\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFigure 2. Retained His-tagged protein binding capacity in presence of EDTA.\u003c\/strong\u003e Ni-Penta 6FF resins were added to 20 volumes of E.coli lysate (~0.5 mg\/mL containing a His-tagged protein of 26 kDa in size), in the presence of 2 mM, 5 mM, 10 mM EDTA respectively. Protein binding was then performed at room temperature for 2 hours. After washing 5 times with 20mM sodium phosphate, 0.5 M NaCl, 5 mM imidazole, pH7.4, the target His-tagged protein was eluted with 20mM sodium phosphate, 0.5 M NaCl, 250 mM imidazole, pH7.4. Protein concentration was measured by UV absorbance at OD280.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eResult:\u003c\/strong\u003e In the presence of 10 mM EDTA, the amount of His-tag protein being purified from Ni-Penta resin was similar to those being purified in the presence of less or no EDTA.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e3. Tolerance to reducing agents\u003c\/h4\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Ni-Penta_9.png?v=1491964108\" alt=\"Ni-penta Tolerance to reducing agents\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eFigure 3. Tolerance to reducing agents. \u003c\/b\u003eNi-Penta 6FF resins were added to 20 volumes of \u003ci\u003eE.coli \u003c\/i\u003elysate (~0.5 mg\/mL containing a His-tagged protein of 26 kDa in size), in the presence of various dilutions of reducing agents, including TCEP, DTT, and β-ME respectively. Protein binding was then performed at room temperature for 2 hours. After washing 5 times with 20mM sodium phosphate, 0.5 M NaCl, 5 mM imidazole, pH7.4, the target His-tagged protein was eluted with 20mM sodium phosphate, 0.5 M NaCl, 250 mM imidazole, pH7.4. Protein concentration was measured by UV absorbance at OD280.\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eResult: \u003c\/b\u003eIn the presence of up to 5 mM TCEP, 10 mM DTT, or 50 mM β-ME, the amount of His-tag protein being purified from Ni-Penta resin was similar to those being purified in absence of the respective reducing agents.\u003c\/p\u003e\n\u003cp\u003e(A) The percentage of retained protein binding capacity is plotted against the concentration of individual reducing agents. (B) Representative SDS-PAGE analysis of the eluates.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e*\u003c\/strong\u003e High Five™ is a trade mark of Thermo Fisher Scientific.\u003c\/p\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"200\"\u003e\u003cstrong\u003eLigand\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eNickel\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBinding capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;10 mg 6XHis-tagged protein\/mL medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMatrix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e4% agarose supplied as 50% slurry\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eParticle size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e45 μm - 165 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaximum pressure\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.1 MPa (1 bar)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eRecommended flow rate\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003eGravity flow, \u0026lt;75 cm\/hour\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eChemical compatibility\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cul\u003e\n\u003cli\u003e0.01 M HCl ,0.01 M NaOH, for ≥ 1 week;\u003c\/li\u003e\n\u003cli\u003e10 mM EDTA, 1 M NaOH, 5 mM DTT, 5 mM TCEP, 20 mM β-ME, 6 M guanidine-HCl, for ≥ 24 hours\u003c\/li\u003e\n\u003cli\u003e500 mM imidazole，100 mM EDTA, for ≥ 2 hours\u003c\/li\u003e\n\u003cli\u003e30% isopropanol for ≥ 20 mins\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage buffer\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1X PBS containing 20% ethanol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProduct is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e   \u003cimg alt=\"Keep refrigerated\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Keep-refrigerated_95fa1867-1f0d-47e6-823a-8a0008076e21_small.png?12332456762384261517\" style=\"float: none;\"\u003e   \u003cimg alt=\"Do not freeze\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Do-not-freeze_2_small.png?12332456762384261517\" style=\"float: none;\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eDocuments\u003c\/h5\u003e\n\u003ch4\u003eProduct Information Sheet\u003c\/h4\u003e\n\u003cp\u003e🗎    \u003ca title=\"Man_11-0227_Chemical-Tolerant Ni-Penta Agarose_v1603.pdf\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Man_11-0227_Chemical-Tolerant_Ni-Penta_Agarose_v1603.pdf?9251540950763704712\" target=\"_blank\"\u003eNi-Penta™ Agarose\u003c\/a\u003e\u003c\/p\u003e\n\u003ch4 style=\"color: #ee8012;\"\u003e\u003cbr\u003e\u003c\/h4\u003e\n\u003cp style=\"text-align: left;\"\u003e \u003c\/p\u003e\n\u003ch5\u003eAccessories\u003c\/h5\u003e\n\u003cp\u003e\u003ca title=\"Disposable gravity flow columns\" href=\"http:\/\/marvelgent.com\/collections\/empty-gravity-flow-columns\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/Gravity_columns_730e2b1d-64df-4e61-96c2-f2f8c076c2d6_compact.png?v=1468002569\" alt=\"\"\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca title=\"Disposable gravity flow columns\" href=\"http:\/\/marvelgent.com\/collections\/empty-gravity-flow-columns\"\u003eDisposable Gravity Flow Columns\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"10 mL","offer_id":17018319684,"sku":"11-0227-010","price":146.0,"currency_code":"USD","in_stock":true},{"title":"50 mL","offer_id":17018319812,"sku":"11-0227-050","price":586.0,"currency_code":"USD","in_stock":true},{"title":"100 mL","offer_id":17018319876,"sku":"11-0227-100","price":1051.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/11-0227_Ni-Penta.png?v=1490835874"},{"product_id":"proteindex-ni-penta-agarose-6ff-chemical-tolerant","title":"PROTEINDEX™ Ni-Penta™ Agarose 6 Fast Flow, Chemical Stable","description":"\u003cp\u003e\u003cstrong\u003ePROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e\u003c\/strong\u003e™ \u003cstrong\u003eChemical-Tolerant Ni-Penta™ Agarose 6FF\u003c\/strong\u003e is a new immobilized metal ion affinity chromatography (IMAC) medium precharged with nickel ions, designed mainly for purification of low abundant proteins or challenging proteins in conditions where EDTA, DTT, β-ME, and other stabilizing agents are necessary for maintaining protein stability and integrity.\u003c\/p\u003e\n\u003cp\u003eThe nickel ion binds very strongly to the matrix support in complex with a proprietary pentadentate chelating ligand, which immobilizes the nickel ion through 5 sites to form a homogeneous,  chemical-stable structure. This characteristic nickel ion bonding on the Ni-Penta™ resin provides excellent resistance to EDTA, NaOH, β-ME, and other reagents that are restricted in the purification protocol using conventional NTA- and IDA- functionalized resins. In particular, Ni-Penta™ Agarose 6FF is well suited for direct capture of secreted polyHis-tagged proteins from the cell culture supernatant of eukaryotic cells, including CHO cells, insect High Five™ cells* and Sf9 cells.\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"http:\/\/marvelgent.com\/pages\/high-selectivity-ni-penta-agarose-resins-for-secreted-proteins\" title=\"Ni-Penta resins for high selective capture of polyHis-tagged proteins\"\u003eRead more on Ni-Penta properties contributing to high selective purification of His-tagged proteins ...\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003eHighly cross-linked 6% agarose as the base matrix support offers excellent mechanical stability for automated, scale up purification of histidine-tagged proteins.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eProduct Highlights\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eEDTA-resistant\u003c\/li\u003e\n\u003cli\u003eHigh selectivity for high purity\u003c\/li\u003e\n\u003cli\u003eRetained high 6xHis affinity in various buffers\u003c\/li\u003e\n\u003cli\u003eRecharge-free with minimal nickel ion leakage\u003c\/li\u003e\n\u003cli\u003eRequires less imidazole\u003c\/li\u003e\n\u003cli\u003eNaOH-tolerant, allowing CIP with 1 M NaOH\u003c\/li\u003e\n\u003cli\u003eVersatile, ready-to-use with convenient formats\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eApplications:\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cstrong\u003ePurification of secreted His-tagged proteins directly from eukaryotic cell culture supernatants\u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003eOwing to its proprietary ligand for nickel ion immobilization, Ni-Penta™ resin tolerates EDTA very well, and its affinity for His-tag can be retained in presence of EDTA up to 10 mM. To purify secreted proteins that are produced from eukaryotic cell cultures (such as insect cells, CHO cells, 293 cells etc.), a step of removing incompatible agents to Ni-NTA resins by dialysis is frequently required prior to loading the material on the column. This is tedious, time-consuming, and labor intensive. By using Ni-Penta resins, sample pretreament can be skipped, and the culture medium can be loaded directly to the column for purification.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cstrong\u003ePurification of His-tagged proteins that require presence of EDTA, DTT, β-ME, and other agents for maximum protein stability and integrity\u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003ePROTEINDEX™ Ni-Penta™ Agarose Resin is compatible with a wide range of buffers including chelating agents (e.g. EDTA) and reducing agents (e.g. DTT), which are incompatible and therefore should be avoid in purification applications using conventional NTA or IDA resins. Ni-Penta™ resin helps to simplify your sample preparation process and allows your target proteins be purified at their preferred conditions.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cstrong\u003eRepetitive uses of the column that require clean-in-place with NaOH but no nickel recharge \u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003eAnti-strip power of the Ni-Penta™ enables repetitive uses of the column without the need to recharge nickel ion, even after multiple cycles of CIP with 1 M NaOH.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cstrong\u003eRestricted imidazole amount in the elution buffer due to the requirements in downstream applications or the target protein's sensitivity to imidazole\u003cbr\u003e\u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003eImidazole is general required to minimize non-specific binding on Ni-NTA resins. Ni-Penta™ Agarose Resin is highly selective for His-tag and therefore does not demand imidazole for minimizing non-specific binding throughout purification. No imidazole is required in the binding buffer, and 40 mM to 100 mM of imidazole can efficiently elute most target proteins.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003e Technical Notes\u003c\/span\u003e\u003c\/h4\u003e\n\u003ch4\u003e 1. EDTA-stable Ni-Penta chemistry for minimal nickel ion leakage\u003c\/h4\u003e\n\u003cp style=\"text-align: center;\"\u003e \u003cimg alt=\"EDTA-stable chelate chemistry for immobilization of nickel ion.\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Ni-Penta_8_grande.png?v=1491963528\" style=\"float: none;\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eFigure 1. Strong chelate chemistry for immobilization of nickel ion. \u003c\/b\u003e\u003c\/p\u003e\n\u003cp\u003eNi-Penta Agarose 6 FF resins were incubated at room temperature for 2 hours in 5 volumes of a serial dilutions of EDTA from 10 mM to 400 mM. The amount of nickel ions released into the buffer was measured.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eResult:\u003c\/strong\u003e During 2 hours of incubation in 50 mM EDTA, less than 5 % of nickel ions leaked from Ni-Penta resins. In comparison, 50 mM EDTA is sufficient to strip off nickel ions from conventional Ni-NTA resins and has been used routinely to regenerate Ni-NTA resin.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e2. Uncompromised His-tag protein binding in EDTA up to 10 mM\u003c\/h4\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"color: #3d85c6;\"\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Ni-Penta_7_654f089b-e6ea-413d-9b06-abb3ead360a8_grande.png?v=1491962523\" style=\"float: none;\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFigure 2. Retained His-tagged protein binding capacity in presence of EDTA.\u003c\/strong\u003e Ni-Penta 6FF resins were added to 20 volumes of E.coli lysate (~0.5 mg\/mL containing a His-tagged protein of 26 kDa in size), in the presence of 2 mM, 5 mM, 10 mM EDTA respectively. Protein binding was then performed at room temperature for 2 hours. After washing 5 times with 20mM sodium phosphate, 0.5 M NaCl, 5 mM imidazole, pH7.4, the target His-tagged protein was eluted with 20mM sodium phosphate, 0.5 M NaCl, 250 mM imidazole, pH7.4. Protein concentration was measured by UV absorbance at OD280.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eResult:\u003c\/strong\u003e In the presence of 10 mM EDTA, the amount of His-tag protein being purified from Ni-Penta resin was similar to those being purified in the presence of less or no EDTA.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e3. Tolerance to reducing agents\u003c\/h4\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Ni-Penta_9.png?v=1491964108\" alt=\"Ni-penta Tolerance to reducing agents\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eFigure 3. Tolerance to reducing agents. \u003c\/b\u003e\u003c\/p\u003e\n\u003cp\u003eNi-Penta 6FF resins were added to 20 volumes of \u003ci\u003eE.coli \u003c\/i\u003e lysate (~0.5 mg\/mL containing a His-tagged protein of 26 kDa in size), in the presence of various dilutions of reducing agents, including TCEP, DTT, and β-ME respectively. Protein binding was then performed at room temperature for 2 hours. After washing 5 times with 20mM sodium phosphate, 0.5 M NaCl, 5 mM imidazole, pH7.4, the target His-tagged protein was eluted with 20mM sodium phosphate, 0.5 M NaCl, 250 mM imidazole, pH7.4. Protein concentration was measured by UV absorbance at OD280.\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eResult: \u003c\/b\u003eIn the presence of up to 5 mM TCEP, 10 mM DTT, or 50 mM β-ME, the amount of His-tag protein being purified from Ni-Penta resin was similar to those being purified in absence of the respective reducing agents.\u003c\/p\u003e\n\u003cp\u003e(A) The percentage of retained protein binding capacity is plotted against the concentration of individual reducing agents. (B) Representative SDS-PAGE analysis of the eluates.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e*\u003c\/strong\u003e High Five™ is a trade mark of Thermo Fisher Scientific.\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"200\"\u003e\u003cstrong\u003eLigand\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eNickel\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBinding capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;10 mg 6XHis-tagged protein\/mL medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMetal ion capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u0026gt; 50 µmol Ni\u003csup\u003e2+ \u003c\/sup\u003e\/ ml medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMatrix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eHighly cross-linked 6% agarose resin supplied as 50% slurry\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eParticle size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e45 μm - 165 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaximum pressure\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.3 MPa (3 bar)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eChemical compatibility\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cul\u003e\n\u003cli\u003e0.01 M HCl ,0.01 M NaOH, for ≥ 1 week;\u003c\/li\u003e\n\u003cli\u003e10 mM EDTA, 1 M NaOH, 5 mM DTT, 5 mM TCEP, 20 mM β-ME, 6 M guanidine-HCl, for ≥ 24 hours\u003c\/li\u003e\n\u003cli\u003e500 mM imidazole，100 mM EDTA, for ≥ 2 hours\u003c\/li\u003e\n\u003cli\u003e30% isopropanol for ≥ 20 mins\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage buffer\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1X PBS containing 20% ethanol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProduct is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e   \u003cimg alt=\"Keep refrigerated\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Keep-refrigerated_95fa1867-1f0d-47e6-823a-8a0008076e21_small.png?12332456762384261517\" style=\"float: none;\"\u003e   \u003cimg alt=\"Do not freeze\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Do-not-freeze_2_small.png?12332456762384261517\" style=\"float: none;\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eDocuments\u003c\/h5\u003e\n\u003ch4\u003eProduct Information Sheet\u003c\/h4\u003e\n\u003cp\u003e🗎    \u003ca title=\"Man_11-0228_Chemical-Tolerant Ni-Penta Agarose6FF_v1603.pdf\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Man_11-0228_Chemical-Tolerant_Ni-Penta_Agarose_6_Fast_Flow_v1603.pdf?4531069748310472707\" target=\"_blank\"\u003eNi-Penta™ Agarose 6FF\u003c\/a\u003e\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"10 mL","offer_id":17033312580,"sku":"11-0228-010","price":146.0,"currency_code":"USD","in_stock":true},{"title":"50 mL","offer_id":17033312644,"sku":"11-0228-050","price":586.0,"currency_code":"USD","in_stock":true},{"title":"100 mL","offer_id":17033312708,"sku":"11-0228-100","price":1051.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/Ni-Penta.png?v=1483497352"},{"product_id":"proteindex-rprotein-l-agarose-4-fast-flow","title":"PROTEINDEX™ rProtein L Agarose 4 Fast Flow","description":"\u003cp\u003e\u003cstrong\u003ePROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e\u003c\/strong\u003e™ rProtein L Agarose 4 Fast Flow is an affinity chromatography medium designed for easy, one-step purification of immunoglobulins, as well as Fab, scFv fragments of immunoglobulins from biological fluids and from cell culture media at both laboratory and process scale.\u003c\/p\u003e\n\u003cp\u003eProtein L is first isolated from the surface of bacterial species \u003cem\u003ePeptostreptoccus magnus \u003c\/em\u003eand now recombinant Protein L is obtained by gene recombination technology. Unlike Protein A and Protein G, which bind primarily through Fc regions of immunoglobulins, Protein L binds the variable region of antibodies kappa light chain and binds a wider range of Ig classes and subclasses. Protein L is therefore particular suitable for capture of a wide range of different-sized antibody fragments such as Fab fragments, single-chain variable fragments (scFv) and domain antibodies (dAbs). It is also suitable for mouse IgM purification from cell.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003ePROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e\u003c\/strong\u003e™ rProtein L Agarose 4 Fast Flow utilizes a stable thioether linkage between rProtein L and the cross-linked agarose beads and is optimized for high binding capacity for immunoglobulins.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"200\"\u003e\u003cstrong\u003eLigand\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eRecombinant protein L produced in \u003cem\u003eE.coli\u003c\/em\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBinding capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;15 mg human IgG\/mL medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMatrix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eCross-linked 4% agarose supplied as a 50% slurry\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eParticle size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e45 μm - 165 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaximum pressure\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.3 MPa (3 bar)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eChemical stability\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eAll commonly used aqueous buffers, including 6 M guanidine-HCl and 8 M urea.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eRecommended flow rate\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eGravity flow and medium pressure, 30 - 300 cm\/hour\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage buffer\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e20% ethanol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProduct is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e   \u003cimg alt=\"Keep refrigerated\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Keep-refrigerated_95fa1867-1f0d-47e6-823a-8a0008076e21_small.png?12332456762384261517\" style=\"float: none;\"\u003e   \u003cimg alt=\"Do not freeze\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Do-not-freeze_2_small.png?12332456762384261517\" style=\"float: none;\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eDocuments\u003c\/h5\u003e\n\u003ch4\u003eTechnical Note\u003c\/h4\u003e\n\u003cp\u003e🗎    \u003ca title=\"Comparison of binding characteristics of immunoglobulin-binding proteins\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Table_Binding_characteristics_of_immunoglobulin-binding_proteins.pdf?6231220717744860621\" rel=\"noopener noreferrer\" target=\"_blank\"\u003eComparison of Binding Characteristics of Immunoglobulin-Binding Proteins\u003c\/a\u003e\u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"5 mL","offer_id":17891846020,"sku":"11-0214-005","price":506.0,"currency_code":"USD","in_stock":true},{"title":"10 mL","offer_id":40574482513963,"sku":"11-0214-010","price":825.0,"currency_code":"USD","in_stock":true},{"title":"25 mL","offer_id":17891846148,"sku":"11-0214-025","price":1842.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/11-0213_Protein_L_Agarose.png?v=1493144974"},{"product_id":"proteindex-ni-penta-agarose-6-fast-flow-chemical-stable-br-prepacked-cartridge","title":"PROTEINDEX™ Ni-Penta™ Agarose 6 Fast Flow, Chemical Stable, Prepacked Cartridge","description":"\u003cp\u003e\u003cstrong\u003eROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e\u003c\/strong\u003e™ \u003cstrong\u003eChemical-Tolerant Ni-Penta™ Agarose 6FF\u003c\/strong\u003e is a new immobilized metal ion affinity chromatography (IMAC) medium precharged with nickel ions, designed mainly for purification of low abundant proteins or challenging proteins in conditions where EDTA, DTT, β-ME, and other stabilizing agents are necessary for maintaining protein stability and integrity.\u003c\/p\u003e\n\u003cp\u003eThe nickel ion binds very strongly to the matrix support in complex with a proprietary pentadentate chelating ligand, which immobilizes the nickel ion through 5 sites to form a homogeneous,  chemical-stable structure. This characteristic nickel ion bonding on the Ni-Penta™ resin provides excellent resistance to EDTA, NaOH, β-ME, and other reagents that are restricted in the purification protocol using conventional NTA- and IDA- functionalized resins. In particular, Ni-Penta™ Agarose 6FF is well suited for direct capture of secreted polyHis-tagged proteins from the cell culture supernatant of eukaryotic cells, including CHO cells, insect High Five™ cells* and Sf9 cells.\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"http:\/\/marvelgent.com\/pages\/high-selectivity-ni-penta-agarose-resins-for-secreted-proteins\" title=\"Ni-Penta resins for high selective capture of polyHis-tagged proteins\"\u003eRead more on Ni-Penta properties contributing to high selective purification of His-tagged proteins ...\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003eNi-Penta™ Agarose 6 FF Prepacked Cartridge are packed with 1 ml and 5 ml of Ni-Penta Agarose 6 Fast Flow. The columns have standard interface that can be adapted to most commonly used chromatography systems, such as ÄKTA.\u003c\/p\u003e\n\u003cp\u003ePrepacked 16\/10 columns and 7\/10 columns offer a bed height of 10 cm that is convenient for process scale-up and development.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eValue bundle with a \u003ca rel=\"noopener\" title=\"Connector Luer Female \/ 10-32 Male in PEEK\" href=\"https:\/\/marvelgent.com\/products\/connector-1-16-luer-female-10-32-male-peek\" target=\"_blank\"\u003eLuer Female\/10-32 Male Connector in PEEK\u003c\/a\u003e (Cat# 20-0315) is now available for all pack sizes, providing convenient operation of the pre-packed column with a syringe at an excellent value.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eProduct Highlights\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eEDTA-resistant\u003c\/li\u003e\n\u003cli\u003eHigh selectivity for high purity\u003c\/li\u003e\n\u003cli\u003eRetained high 6xHis affinity in various buffers\u003c\/li\u003e\n\u003cli\u003eRecharge-free with minimal nickel ion leakage\u003c\/li\u003e\n\u003cli\u003eRequires less imidazole\u003c\/li\u003e\n\u003cli\u003eNaOH-tolerant, allowing CIP with 1 M NaOH\u003c\/li\u003e\n\u003cli\u003eReady-to-use, convenient formats: 1 mL and 5 mL\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eApplications:\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cstrong\u003ePurification of secreted His-tagged proteins directly from eukaryotic cell culture supernatants\u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003eOwing to its proprietary ligand for nickel ion immobilization, Ni-Penta™ resin tolerates EDTA very well, and its affinity for His-tag can be retained in presence of EDTA up to 10 mM. To purify secreted proteins that are produced from eukaryotic cell cultures (such as insect cells, CHO cells, 293 cells etc.), a step of removing incompatible agents to Ni-NTA resins by dialysis is frequently required prior to loading the material on the column. This is tedious, time-consuming, and labor intensive. By using Ni-Penta resins, sample pretreament can be skipped, and the culture medium can be loaded directly to the column for purification.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cstrong\u003ePurification of His-tagged proteins that require presence of EDTA, DTT, β-ME, and other agents for maximum protein stability and integrity\u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003ePROTEINDEX™ Ni-Penta™ Agarose Resin is compatible with a wide range of buffers including chelating agents (e.g. EDTA) and reducing agents (e.g. DTT), which are incompatible and therefore should be avoid in purification applications using conventional NTA or IDA resins. Ni-Penta™ resin helps to simplify your sample preparation process and allows your target proteins be purified at their preferred conditions.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cstrong\u003eRepetitive uses of the column that require clean-in-place with NaOH but no nickel recharge \u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003eAnti-strip power of the Ni-Penta™ enables repetitive uses of the column without the need to recharge nickel ion, even after multiple cycles of CIP with 1 M NaOH.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cstrong\u003eRestricted imidazole amount in the elution buffer due to the requirements in downstream applications or the target protein's sensitivity to imidazole\u003cbr\u003e\u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003eImidazole is general required to minimize non-specific binding on Ni-NTA resins. Ni-Penta™ Agarose Resin is highly selective for His-tag and therefore does not demand imidazole for minimizing non-specific binding throughout purification. No imidazole is required in the binding buffer, and 40 mM to 100 mM of imidazole can efficiently elute most target proteins.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\n\u003cspan style=\"color: #3d85c6;\"\u003e Technical Notes\u003c\/span\u003e \u003c\/h4\u003e\n\u003ch4\u003e1. EDTA-stable Ni-Penta chemistry for minimal nickel ion leakage\u003c\/h4\u003e\n\u003cp style=\"text-align: center;\"\u003e \u003cimg alt=\"EDTA-stable chelate chemistry for immobilization of nickel ion.\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Ni-Penta_8_grande.png?v=1491963528\" style=\"float: none;\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eFigure 1. Strong chelate chemistry for immobilization of nickel ion. \u003c\/b\u003eNi-Penta Agarose 6 FF resins were incubated at room temperature for 2 hours in 5 volumes of a serial dilutions of EDTA from 10 mM to 400 mM. The amount of nickel ions released into the buffer was measured.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eResult:\u003c\/strong\u003e During 2 hours of incubation in 50 mM EDTA, less than 5 % of nickel ions leaked from Ni-Penta resins. In comparison, 50 mM EDTA is sufficient to strip off nickel ions from conventional Ni-NTA resins and has been used routinely to regenerate Ni-NTA resin.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e2. Uncompromised His-tag protein binding in EDTA up to 10 mM\u003c\/h4\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cspan style=\"color: #3d85c6;\"\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Ni-Penta_7_654f089b-e6ea-413d-9b06-abb3ead360a8_grande.png?v=1491962523\" style=\"float: none;\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFigure 2. Retained His-tagged protein binding capacity in presence of EDTA.\u003c\/strong\u003e Ni-Penta 6FF resins were added to 20 volumes of E.coli lysate (~0.5 mg\/mL containing a His-tagged protein of 26 kDa in size), in the presence of 2 mM, 5 mM, 10 mM EDTA respectively. Protein binding was then performed at room temperature for 2 hours. After washing 5 times with 20mM sodium phosphate, 0.5 M NaCl, 5 mM imidazole, pH7.4, the target His-tagged protein was eluted with 20mM sodium phosphate, 0.5 M NaCl, 250 mM imidazole, pH7.4. Protein concentration was measured by UV absorbance at OD280.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eResult:\u003c\/strong\u003e In the presence of 10 mM EDTA, the amount of His-tag protein being purified from Ni-Penta resin was similar to those being purified in the presence of less or no EDTA.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e3. Tolerance to reducing agents\u003c\/h4\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Ni-Penta_9.png?v=1491964108\" alt=\"Ni-penta Tolerance to reducing agents\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eFigure 3. Tolerance to reducing agents. \u003c\/b\u003eNi-Penta 6FF resins were added to 20 volumes of \u003ci\u003eE.coli \u003c\/i\u003elysate (~0.5 mg\/mL containing a His-tagged protein of 26 kDa in size), in the presence of various dilutions of reducing agents, including TCEP, DTT, and β-ME respectively. Protein binding was then performed at room temperature for 2 hours. After washing 5 times with 20mM sodium phosphate, 0.5 M NaCl, 5 mM imidazole, pH7.4, the target His-tagged protein was eluted with 20mM sodium phosphate, 0.5 M NaCl, 250 mM imidazole, pH7.4. Protein concentration was measured by UV absorbance at OD280.\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eResult: \u003c\/b\u003eIn the presence of up to 5 mM TCEP, 10 mM DTT, or 50 mM β-ME, the amount of His-tag protein being purified from Ni-Penta resin was similar to those being purified in absence of the respective reducing agents.\u003c\/p\u003e\n\u003cp\u003e(A) The percentage of retained protein binding capacity is plotted against the concentration of individual reducing agents. (B) Representative SDS-PAGE analysis of the eluates.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e*\u003c\/strong\u003e High Five™ is a trade mark of Thermo Fisher Scientific.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"200\"\u003e\u003cstrong\u003eLigand\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eNickel\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBinding capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;10 mg 6XHis-tagged protein\/mL medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMatrix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eHighly cross-linked 6% agarose resin\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eParticle size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e45 μm - 165 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaximum pressure\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.3 MPa (3 bar)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eChemical compatibility\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cul\u003e\n\u003cli\u003e0.01 M HCl ,0.01 M NaOH, for ≥ 1 week;\u003c\/li\u003e\n\u003cli\u003e10 mM EDTA, 1 M NaOH, 5 mM DTT, 5 mM TCEP, 20 mM β-ME, 6 M guanidine-HCl, for ≥ 24 hours\u003c\/li\u003e\n\u003cli\u003e500 mM imidazole，100 mM EDTA, for ≥ 2 hours\u003c\/li\u003e\n\u003cli\u003e30% isopropanol for ≥ 20 mins\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage buffer\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1x PBS containing 20% ethanol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProduct is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e   \u003cimg alt=\"Keep refrigerated\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Keep-refrigerated_95fa1867-1f0d-47e6-823a-8a0008076e21_small.png?12332456762384261517\" style=\"float: none;\"\u003e   \u003cimg alt=\"Do not freeze\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Do-not-freeze_2_small.png?12332456762384261517\" style=\"float: none;\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eDocuments\u003c\/h5\u003e\n\u003ch4\u003eProduct Information Sheet\u003c\/h4\u003e\n\u003cp\u003e🗎    \u003ca title=\"Man_11-0228_Chemical-Tolerant Ni-Penta Agarose6FF Prepacked Cartridges_v1603.pdf\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Man_11-0229_Chemical-Tolerant_Ni-Penta_Agarose_6_FF_Prepacked_Cartridge_v1603.pdf?9251540950763704712\" rel=\"noopener noreferrer\" target=\"_blank\"\u003eNi-Penta™ Agarose 6FF, Prepacked Cartridges\u003c\/a\u003e\u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"1 mL Columns, Pack of 5","offer_id":41405565042731,"sku":"11-0229-5x1ML","price":177.0,"currency_code":"USD","in_stock":true},{"title":"1 mL Columns, Pack of 5 with 1 Connector","offer_id":41405559537707,"sku":"11-0229-5x1MLB","price":204.0,"currency_code":"USD","in_stock":true},{"title":"5 mL Column, Pack of 1","offer_id":41405388554283,"sku":"11-0229-1x5ML","price":151.0,"currency_code":"USD","in_stock":true},{"title":"5 mL Column, Pack of 1,  with 1 Connector","offer_id":41405562585131,"sku":"11-0229-1x5MLB","price":179.0,"currency_code":"USD","in_stock":true},{"title":"5 mL Columns, Pack of 5","offer_id":41405565173803,"sku":"11-0229-5x5ML","price":586.0,"currency_code":"USD","in_stock":true},{"title":"5 mL Columns, Pack of 5, with 1 Connector","offer_id":41405388619819,"sku":"11-0229-5x5MLB","price":613.0,"currency_code":"USD","in_stock":true},{"title":"16\/10 Column, 20 mL","offer_id":42112519569451,"sku":"11-0229-C20","price":709.0,"currency_code":"USD","in_stock":true},{"title":"7\/10 Columns, 4.7 mL, Pack of 2","offer_id":42112598048811,"sku":"11-0229-C47","price":544.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/11-0229_Ni-Penta_cartridges.png?v=1493997900"},{"product_id":"proteindex-ni-nta-agarose","title":"PROTEINDEX™ HiBond™ Ni-NTA Agarose","description":"\u003cp\u003e\u003cstrong\u003ePROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e™ HiBond™ Ni-NTA Agarose \u003c\/strong\u003eutilizes 4% agarose as the matrix support, and is functionalized with the chelate ligand Nitilotriacetic Acid (NTA), which is charged with nickel ions (Ni\u003csup\u003e2+\u003c\/sup\u003e) to form a very stable octahedral structure with the nickel ion in the center. The structure of Ni-NTA complex is compatible with solutions that contain certain concentration of reducing agents, denaturing agents, detergents and other additives.\u003c\/p\u003e\n\u003cp\u003ePROTEINDEX™ HiBond™ Ni-NTA Agarose can be used to purify 6xHis-tagged proteins expressed in various hosts, such as\u003cem\u003e E.coli\u003c\/em\u003e., yeast, insect cells and mammalian cells.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eProduct Highlights\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eSuited for routine purification of histidine-tagged proteins\u003c\/li\u003e\n\u003cli\u003eHigh yield (typically \u0026gt; 40 mg protein \/mL resins)\u003c\/li\u003e\n\u003cli\u003eLow leakage of nickel ions. Can be re-used for up to 8 preps*\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cspan style=\"color: #666666;\"\u003e*Note: No significant decrease in yield after 8 repetitive uses of resin when recommended buffers are used. Refer to Product Details for compatible reagents.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eApplications\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eManual batch or gravity flow purification\u003c\/li\u003e\n\u003cli\u003eRoutine His tag protein purification for structural biology and functional assays\u003c\/li\u003e\n\u003cli\u003eProtein-protein and protein-nucleic acid interaction studies\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003e\u003c\/span\u003e\u003c\/h4\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eTech Notes\u003c\/span\u003e\u003c\/h4\u003e\n\u003cp\u003e\u003cb\u003eOne-step purification using \u003c\/b\u003e\u003cb\u003eP\u003c\/b\u003e\u003cb\u003eroteIndex\u003c\/b\u003e\u003cb\u003e™ \u003c\/b\u003e\u003cb\u003eHiBond\u003c\/b\u003e\u003cb\u003e™ Ni-NTA \u003c\/b\u003e\u003cb\u003eAgarose 6 Fast Flow resins \u003c\/b\u003e\u003c\/p\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 0px; border-color: #ffffff;\" width=\"30%\"\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Ni-NTA_2.png?v=1496932129\" alt=\"\"\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 0px; border-color: #ffffff;\" width=\"30%\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cstrong\u003eFigure 1. \u003c\/strong\u003eE.coli lysate containing a 50 kDa target protein was directly loaded on HiBond™ Ni-NTA Agarose 6 FF column. The column was then washed sequentially with 0 mM, 5 mM, and 20 mM imidazole solution before elution with 300 mM imidazole. The target protein was analyzed by SDS-PAGE. \u003cb\u003eC\u003c\/b\u003e\u003cb\u003eL\u003c\/b\u003e: raw lysate; \u003cb\u003eFT\u003c\/b\u003e: unbound flow-through; \u003cb\u003eW1\u003c\/b\u003e,\u003cb\u003e W2 \u003c\/b\u003eand \u003cb\u003eW3: \u003c\/b\u003ewashes; \u003cb\u003eE1\u003c\/b\u003e and \u003cb\u003eE2\u003c\/b\u003e: eluate fractions.\n\u003cul\u003e\u003c\/ul\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"200\"\u003e\u003cstrong\u003eLigand\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eNickel\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBinding capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;40 mg 6xHis-tagged protein\/mL medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMatrix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e4% agarose supplied as 50% slurry\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eParticle size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e45 μm - 165 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaximum pressure\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.1 MPa (1 bar)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eRecommended flow rate\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003eGravity flow, \u0026lt;75 cm\/hour\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eChemical compatibility\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003ctable width=\"93%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"37%\" valign=\"top\"\u003eReducing agents\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e5 mM DTE\u003cbr\u003e0.5-1mM DTT\u003cbr\u003e20 mM β-mercaptoethanol\u003cbr\u003e5 mM TCEP\u003cbr\u003e10 mM reduced glutathione (GSH)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"37%\" valign=\"top\"\u003eDenaturing agents\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e8 M urea, 6 M Gua-HCl \u003cbr\u003e(Tested for one week at 40°C)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"37%\" valign=\"top\"\u003eDetergents\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e2% Triton™ X-100 (nonionic)\u003cbr\u003e2% Tween™ 20 (nonionic)\u003cbr\u003e2% NP-40 (nonionic)\u003cbr\u003e2% cholate (anionic)\u003cbr\u003e1% CHAPS (zwitterionic)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"37%\" valign=\"top\"\u003eOther additives\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e500 mM imidazole\u003cbr\u003e20% ethanol\u003cbr\u003e50% glycerol\u003cbr\u003e100 mM Na\u003csub\u003e2\u003c\/sub\u003eSO4\u003cbr\u003e1.5 M NaCl\u003cbr\u003e1 mM EDTA\u003cbr\u003e60 mM citrate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"37%\" valign=\"top\"\u003eCommonly used buffer\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e50 mM sodium phosphate, pH 7.4\u003cbr\u003e100 mM Tris-HCl, pH 7.4\u003cbr\u003e100 mM Tris-acetate, pH 7.4\u003cbr\u003e100 mM HEPES, pH 7.4\u003cbr\u003e100 mM MOPS, pH 7.4\u003cbr\u003e100 mM sodium acetate, pH 4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage buffer\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1x PBS containing 20% ethanol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProduct is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e   \u003cimg alt=\"Keep refrigerated\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Keep-refrigerated_95fa1867-1f0d-47e6-823a-8a0008076e21_small.png?12332456762384261517\" style=\"float: none;\"\u003e   \u003cimg alt=\"Do not freeze\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Do-not-freeze_2_small.png?12332456762384261517\" style=\"float: none;\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003e\u003c\/h5\u003e\n\u003ch4 style=\"color: #ee8012;\"\u003e\u003c\/h4\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"10 mL","offer_id":18927593668,"sku":"11-0224-010","price":115.0,"currency_code":"USD","in_stock":true},{"title":"50 mL","offer_id":18927593732,"sku":"11-0224-050","price":412.0,"currency_code":"USD","in_stock":true},{"title":"100 mL","offer_id":18927593796,"sku":"11-0224-100","price":759.0,"currency_code":"USD","in_stock":true},{"title":"500 mL","offer_id":39291740979243,"sku":"11-0224-500","price":2733.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/11-0224_Ni-NTA.png?v=1483498777"},{"product_id":"proteindex-ni-nta-agarose-6ff","title":"PROTEINDEX™ HiBond™ Ni-NTA Agarose 6FF","description":"\u003cp\u003e\u003cstrong\u003ePROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e\u003c\/strong\u003e™ \u003cb\u003eHiBond\u003c\/b\u003e\u003cb\u003e™ \u003c\/b\u003e\u003cstrong\u003eNi-NTA Agarose\u003c\/strong\u003e \u003cstrong\u003e6FF\u003c\/strong\u003e has been optimized for automated purification applications. It has been widely used in scale-up and preparative purification of histidine-tagged proteins that are expressed in various hosts, such as \u003cem\u003eE.coli\u003c\/em\u003e, yeast, insect cells, and mammalian cells.\u003c\/p\u003e\n\u003cp\u003e        The base matrix of PROTEINDEX™ HiBond™ Ni-NTA Agarose 6FF is highly crosslinked 6% agarose, offering excellent chemical-, and mechanical stability for automated applications. The matrix support is functionalized with the chelate ligand Nitilotriacetic Acid (NTA) in complex with the nickel ions (Ni\u003csup\u003e2+\u003c\/sup\u003e) in a very stable octahedral structure. The structure of Ni-NTA complex is compatible solutions that contain certain concentration of reducing agents, denaturing agents, detergents and other additives.\u003c\/p\u003e\n\u003cp\u003e        The NTA ligand coordinates the Ni\u003csup\u003e2+\u003c\/sup\u003e with four valencies (tetradentate, coordination number 4), and two valencies are available for interaction with imidazole rings of histidine residues. The number of available valencies seems to provide the best combination of binding effectiveness and chemical stability for binding with the His tag. The Ni-NTA ion-chelate complex on our ProteIndex™ HiBond™ Ni-NTA resins has been delicately optimized to ensure the most effective capture for His tag purification of His-tagged proteins. \u003c\/p\u003e\n\u003cp\u003e       \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eProduct Highlights\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eMechanical stable, designed for scaling up His tag purification\u003c\/li\u003e\n\u003cli\u003eHigh yield (typically \u0026gt; 40 mg protein \/mL resins)\u003c\/li\u003e\n\u003cli\u003eLow leakage of nickel ions. Can be re-used for up to 8 preps*\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cspan style=\"color: #666666;\"\u003e*Note: No significant decrease in yield after 8 repetitive uses of resin when recommended buffers are used. Refer to Product Details for compatible reagents.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eApplications\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eAutomated or syringe operations; and manual batch or gravity flow\u003c\/li\u003e\n\u003cli\u003eRoutine His tag protein purification for structural biology and functional assays\u003c\/li\u003e\n\u003cli\u003eScaling up and preparative applications\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eTech Notes\u003c\/span\u003e\u003c\/h4\u003e\n\u003cp\u003e\u003cb\u003eOne-step purification using \u003c\/b\u003e\u003cb\u003eP\u003c\/b\u003e\u003cb\u003eroteIndex\u003c\/b\u003e\u003cb\u003e™ \u003c\/b\u003e\u003cb\u003eHiBond\u003c\/b\u003e\u003cb\u003e™ Ni-NTA \u003c\/b\u003e\u003cb\u003eAgarose 6 Fast Flow resins \u003c\/b\u003e\u003c\/p\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 0px; border-color: #ffffff;\" width=\"35%\"\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Ni-NTA_6FF_2.png?v=1496201543\" alt=\"\" style=\"display: block; margin-left: auto; margin-right: auto;\"\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 0px; border-color: #ffffff;\" width=\"35%\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cstrong\u003eFigure 1.\u003c\/strong\u003e A 15 kDa protein was expressed in E.coli and purified on an automated system using \u003cb\u003eP\u003c\/b\u003e\u003cb\u003eroteIndex\u003c\/b\u003e\u003cb\u003e™ \u003c\/b\u003e\u003cb\u003eHiBond\u003c\/b\u003e\u003cb\u003e™ Ni-NTA \u003c\/b\u003e\u003cb\u003eAgarose 6 Fast Flow resin \u003c\/b\u003eunder native conditions. 50 mL E.coli lysate in 50 mM NaH\u003csub\u003e2\u003c\/sub\u003ePO\u003csub\u003e4\u003c\/sub\u003e, 0.3 M NaCl, 10 mM imidazole was loaded on the column packed with 0.5 mL resins at the speed of 1 mL\/min. The column was then washed with 100 mL 50 mM NaH\u003csub\u003e2\u003c\/sub\u003ePO\u003csub\u003e4\u003c\/sub\u003e, 0.3 M NaCl, 20 mM imidazole at the speed of 1~2 mL\/min. The target protein was eluted with 50 mM NaH\u003csub\u003e2\u003c\/sub\u003ePO\u003csub\u003e4\u003c\/sub\u003e, 0.3 M NaCl, 250 mM imidazole. Proteins were visualized by coomassie blue staining after separation on SDS-PAGE. \u003cb\u003eL\u003c\/b\u003e: raw lysate; \u003cb\u003eF\u003c\/b\u003e: unbound flow-through; \u003cb\u003eE1\u003c\/b\u003e and \u003cb\u003eE2\u003c\/b\u003e: eluate fractions.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"border: 0px; border-color: #ffffff;\" width=\"37%\"\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Ni-NTA_6FF_comparison_with_Ni_sepharose_4.png?v=1497317795\" alt=\"\"\u003e\u003c\/td\u003e\n\u003ctd style=\"border: 0px; border-color: #ffffff;\" width=\"37%\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cstrong\u003eFigure 2.\u003c\/strong\u003e A 25 kDa protein was expressed in E.coli and purified on an automated system using a one-step automated protocol with \u003cb\u003eP\u003c\/b\u003e\u003cb\u003eroteIndex\u003c\/b\u003e\u003cb\u003e™ \u003c\/b\u003e\u003cb\u003eHiBond\u003c\/b\u003e\u003cb\u003e™ Ni-NTA \u003c\/b\u003e\u003cb\u003eAgarose 6 Fast Flow resin \u003c\/b\u003eunder native conditions. 50 mL E.coli lysate in 50 mM NaH\u003csub\u003e2\u003c\/sub\u003ePO\u003csub\u003e4\u003c\/sub\u003e, 0.3 M NaCl, 10 mM imidazole was loaded on the column packed with 0.5 mL resins at the speed of 1 mL\/min. The column was then washed with 100 mL 50 mM NaH\u003csub\u003e2\u003c\/sub\u003ePO\u003csub\u003e4\u003c\/sub\u003e, 0.3 M NaCl, 20 mM imidazole at the speed of 1~2 mL\/min. The target protein was eluted with 50 mM NaH\u003csub\u003e2\u003c\/sub\u003ePO\u003csub\u003e4\u003c\/sub\u003e, 0.3 M NaCl, 250 mM imidazole. A fraction of eluate was analyzed on a SDS-PAGE. Shown were coomassie blue stains of the target proteins purified from columns of Marvelgent’s ProteIndex Ni-NTA 6FF (\u003cstrong\u003eM\u003c\/strong\u003e) and Ni-Sepharose (\u003cstrong\u003eG\u003c\/strong\u003e) resins.\u003cbr\u003e\u003c\/p\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"200\"\u003e\u003cstrong\u003eLigand\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eNickel\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBinding capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u0026gt; 40 mg 6xHis-tagged protein\/mL medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMatrix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e6% highly cross-linked agarose supplied as 50% slurry\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eParticle size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e45 μm - 165 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaximum pressure\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.3 MPa (3 bar)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eChemical compatibility\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003ctable width=\"93%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"37%\" valign=\"top\"\u003eReducing agents\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e5 mM DTE\u003cbr\u003e0.5-1 mM DTT\u003cbr\u003e20 mM β-mercaptoethanol\u003cbr\u003e5 mM TCEP\u003cbr\u003e10 mM reduced glutathione (GSH)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"37%\" valign=\"top\"\u003eDenaturing agents\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e8 M urea, 6 M Gua-HCl \u003cbr\u003e(Tested for one week at 40°C)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"37%\" valign=\"top\"\u003eDetergents\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e2% Triton™ X-100 (nonionic)\u003cbr\u003e2% Tween™ 20 (nonionic)\u003cbr\u003e2% NP-40 (nonionic)\u003cbr\u003e2% cholate (anionic)\u003cbr\u003e1% CHAPS (zwitterionic)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"37%\" valign=\"top\"\u003eOther additives\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e500 mM imidazole\u003cbr\u003e20% ethanol\u003cbr\u003e50% glycerol\u003cbr\u003e100 mM Na\u003csub\u003e2\u003c\/sub\u003eSO4\u003cbr\u003e1.5 M NaCl\u003cbr\u003e1 mM EDTA\u003cbr\u003e60 mM citrate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"37%\" valign=\"top\"\u003eCommonly used buffer\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e50 mM sodium phosphate, pH 7.4\u003cbr\u003e100 mM Tris-HCl, pH 7.4\u003cbr\u003e100 mM Tris-acetate, pH 7.4\u003cbr\u003e100 mM HEPES, pH 7.4\u003cbr\u003e100 mM MOPS, pH 7.4\u003cbr\u003e100 mM sodium acetate, pH 4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage buffer\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1x PBS containing 20% ethanol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProduct is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e   \u003cimg alt=\"Keep refrigerated\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Keep-refrigerated_95fa1867-1f0d-47e6-823a-8a0008076e21_small.png?12332456762384261517\" style=\"float: none;\"\u003e   \u003cimg alt=\"Do not freeze\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Do-not-freeze_2_small.png?12332456762384261517\" style=\"float: none;\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003e\u003cbr\u003e\u003c\/h5\u003e\n\u003ch4 style=\"color: #ee8012;\"\u003e\u003cbr\u003e\u003c\/h4\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"10 mL","offer_id":19000297540,"sku":"11-0225-010","price":126.0,"currency_code":"USD","in_stock":true},{"title":"50 mL","offer_id":19000297604,"sku":"11-0225-050","price":506.0,"currency_code":"USD","in_stock":true},{"title":"100 mL","offer_id":19000297668,"sku":"11-0225-100","price":825.0,"currency_code":"USD","in_stock":true},{"title":"500 mL","offer_id":5704109129767,"sku":"11-0225-500","price":3366.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/11-0225_Ni-NTA_6FF.png?v=1496933028"},{"product_id":"proteindex-ni-nta-agarose-6ff-prepacked-cartridge","title":"PROTEINDEX™ HiBond™ Ni-NTA Agarose 6FF, Prepacked Cartridge","description":"\u003cp\u003e\u003cstrong\u003ePROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e\u003c\/strong\u003e™ \u003cb\u003eHiBond\u003c\/b\u003e\u003cb\u003e™ \u003c\/b\u003e\u003cstrong\u003eNi-NTA Agarose\u003c\/strong\u003e \u003cstrong\u003e6FF\u003c\/strong\u003e has been optimized for automated purification applications. It has been widely used in scale-up and preparative purification of histidine-tagged proteins that are expressed in various hosts, such as \u003cem\u003eE.coli\u003c\/em\u003e, yeast, insect cells, and mammalian cells.\u003c\/p\u003e\n\u003cp\u003e        The base matrix of PROTEINDEX™ HiBond™ Ni-NTA Agarose 6FF is highly crosslinked 6% agarose, offering excellent chemical-, and mechanical stability for automated applications. The matrix support is functionalized with the chelate ligand Nitilotriacetic Acid (NTA) in complex with the nickel ions (Ni\u003csup\u003e2+\u003c\/sup\u003e) in a very stable octahedral structure. The structure of Ni-NTA complex is compatible solutions that contain certain concentration of reducing agents, denaturing agents, detergents and other additives.\u003c\/p\u003e\n\u003cp\u003e        The NTA ligand coordinates the Ni\u003csup\u003e2+\u003c\/sup\u003e with four valencies (tetradentate, coordination number 4), and two valencies are available for interaction with imidazole rings of histidine residues. The number of available valencies seems to provide the best combination of binding effectiveness and chemical stability for binding with the His tag. The Ni-NTA ion-chelate complex on our ProteIndex™ HiBond™ Ni-NTA resins has been delicately optimized to ensure the most effective capture for His tag purification of His-tagged proteins. \u003c\/p\u003e\n\u003cp\u003e        The Prepacked Cartridge has standard interface and can be adapted to most commonly used chromatography systems, such as ÄKTA systems.\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e        Value bundle with a \u003c\/span\u003e\u003ca rel=\"noopener\" title=\"Connector Luer Female \/ 10-32 Male in PEEK\" href=\"https:\/\/marvelgent.com\/products\/connector-1-16-luer-female-10-32-male-peek\" target=\"_blank\"\u003eLuer Female\/10-32 Male Connector in PEEK\u003c\/a\u003e\u003cspan\u003e (Cat# 20-0315) is now available for all pack sizes, providing convenient operation of the pre-packed column with a syringe at an excellent value.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eProduct Highlights\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eConvenient, easy, ready-to-use\u003c\/li\u003e\n\u003cli\u003eMechanical stable, designed for large scale purification of histidine-tagged proteins\u003c\/li\u003e\n\u003cli\u003eHigh yield (typically \u0026gt; 40 mg protein \/mL resins)\u003c\/li\u003e\n\u003cli\u003eLow leakage of nickel ions. Can be re-used for up to 8 preps*\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cspan style=\"color: #666666;\"\u003e*Note: No significant decrease in yield after 8 repetitive uses of resin when recommended buffers are used. Refer to Product Details for compatible reagents.\u003c\/span\u003e\u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eApplications\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eAutomated purifications\u003c\/li\u003e\n\u003cli\u003eRoutine His tag protein purification for structural biology and functional assays\u003c\/li\u003e\n\u003cli\u003eScaling up and preparative applications\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eTech Notes\u003c\/span\u003e\u003c\/h4\u003e\n\u003cp\u003e\u003cb\u003eOne-step purification using \u003c\/b\u003e\u003cb\u003eP\u003c\/b\u003e\u003cb\u003eroteIndex\u003c\/b\u003e\u003cb\u003e™ \u003c\/b\u003e\u003cb\u003eHiBond\u003c\/b\u003e\u003cb\u003e™ Ni-NTA \u003c\/b\u003e\u003cb\u003eAgarose 6 Fast Flow resins \u003c\/b\u003e\u003c\/p\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"35%\" style=\"border: 0px; border-color: #ffffff;\"\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Ni-NTA_6FF_2.png?v=1496201543\"\u003e\u003c\/td\u003e\n\u003ctd width=\"35%\" style=\"border: 0px; border-color: #ffffff;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cstrong\u003eFigure 1.\u003c\/strong\u003e A 15 kDa protein was expressed in E.coli and purified on an automated system using \u003cb\u003eP\u003c\/b\u003e\u003cb\u003eroteIndex\u003c\/b\u003e\u003cb\u003e™ \u003c\/b\u003e\u003cb\u003eHiBond\u003c\/b\u003e\u003cb\u003e™ Ni-NTA \u003c\/b\u003e\u003cb\u003eAgarose 6 Fast Flow resin \u003c\/b\u003eunder native conditions. 50 mL E.coli lysate in 50 mM NaH\u003csub\u003e2\u003c\/sub\u003ePO\u003csub\u003e4\u003c\/sub\u003e, 0.3 M NaCl, 10 mM imidazole was loaded on the column packed with 0.5 mL resins at the speed of 1 mL\/min. The column was then washed with 100 mL 50 mM NaH\u003csub\u003e2\u003c\/sub\u003ePO\u003csub\u003e4\u003c\/sub\u003e, 0.3 M NaCl, 20 mM imidazole at the speed of 1~2 mL\/min. The target protein was eluted with 50 mM NaH\u003csub\u003e2\u003c\/sub\u003ePO\u003csub\u003e4\u003c\/sub\u003e, 0.3 M NaCl, 250 mM imidazole. Proteins were visualized by coomassie blue staining after separation on SDS-PAGE. \u003cb\u003eL\u003c\/b\u003e: raw lysate; \u003cb\u003eF\u003c\/b\u003e: unbound flow-through; \u003cb\u003eE1\u003c\/b\u003e and \u003cb\u003eE2\u003c\/b\u003e: eluate fractions.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"37%\" style=\"border: 0px; border-color: #ffffff;\"\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Ni-NTA_6FF_comparison_with_Ni_sepharose_4.png?v=1497317795\"\u003e\u003c\/td\u003e\n\u003ctd width=\"37%\" style=\"border: 0px; border-color: #ffffff;\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cstrong\u003eFigure 2.\u003c\/strong\u003e A 25 kDa protein was expressed in E.coli and purified on an automated system using a one-step automated protocol with \u003cb\u003eP\u003c\/b\u003e\u003cb\u003eroteIndex\u003c\/b\u003e\u003cb\u003e™ \u003c\/b\u003e\u003cb\u003eHiBond\u003c\/b\u003e\u003cb\u003e™ Ni-NTA \u003c\/b\u003e\u003cb\u003eAgarose 6 Fast Flow resin \u003c\/b\u003eunder native conditions. 50 mL E.coli lysate in 50 mM NaH\u003csub\u003e2\u003c\/sub\u003ePO\u003csub\u003e4\u003c\/sub\u003e, 0.3 M NaCl, 10 mM imidazole was loaded on the column packed with 0.5 mL resins at the speed of 1 mL\/min. The column was then washed with 100 mL 50 mM NaH\u003csub\u003e2\u003c\/sub\u003ePO\u003csub\u003e4\u003c\/sub\u003e, 0.3 M NaCl, 20 mM imidazole at the speed of 1~2 mL\/min. The target protein was eluted with 50 mM NaH\u003csub\u003e2\u003c\/sub\u003ePO\u003csub\u003e4\u003c\/sub\u003e, 0.3 M NaCl, 250 mM imidazole. A fraction of eluate was analyzed on a SDS-PAGE. Shown were coomassie blue stains of the target proteins purified from columns of Marvelgent’s ProteIndex Ni-NTA 6FF (\u003cstrong\u003eM\u003c\/strong\u003e) and Ni-Sepharose (\u003cstrong\u003eG\u003c\/strong\u003e) resins.\u003c\/p\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"200\"\u003e\u003cstrong\u003eLigand\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eNickel\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBinding capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;40 mg 6xHis-tagged protein\/mL medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMatrix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e6% highly cross-linked agarose\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eParticle size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e45 μm - 165 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eColumn volume\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1 mL or 5 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaximum pressure\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.3 MPa (3 bar)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eChemical compatibility\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003ctable width=\"93%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"37%\"\u003eReducing agents\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e5 mM DTE\u003cbr\u003e0.5-1 mM DTT\u003cbr\u003e20 mM β-mercaptoethanol\u003cbr\u003e5 mM TCEP\u003cbr\u003e10 mM reduced glutathione (GSH)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"37%\"\u003eDenaturing agents\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e8 M urea, 6 M Gua-HCl \u003cbr\u003e(Tested for one week at 40°C)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"37%\"\u003eDetergents\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e2% Triton™ X-100 (nonionic)\u003cbr\u003e2% Tween™ 20 (nonionic)\u003cbr\u003e2% NP-40 (nonionic)\u003cbr\u003e2% cholate (anionic)\u003cbr\u003e1% CHAPS (zwitterionic)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"37%\"\u003eOther additives\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e500 mM imidazole\u003cbr\u003e20% ethanol\u003cbr\u003e50% glycerol\u003cbr\u003e100 mM Na\u003csub\u003e2\u003c\/sub\u003eSO4\u003cbr\u003e1.5 M NaCl\u003cbr\u003e1 mM EDTA\u003cbr\u003e60 mM citrate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"37%\"\u003eCommonly used buffer\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e50 mM sodium phosphate, pH 7.4\u003cbr\u003e100 mM Tris-HCl, pH 7.4\u003cbr\u003e100 mM Tris-acetate, pH 7.4\u003cbr\u003e100 mM HEPES, pH 7.4\u003cbr\u003e100 mM MOPS, pH 7.4\u003cbr\u003e100 mM sodium acetate, pH 4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eColumn hardware pressure limit\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003e5 bar (0.5 MPa)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage buffer\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1x PBS containing 20% ethanol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProduct is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e   \u003cimg style=\"float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Keep-refrigerated_95fa1867-1f0d-47e6-823a-8a0008076e21_small.png?12332456762384261517\" alt=\"Keep refrigerated\"\u003e   \u003cimg style=\"float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Do-not-freeze_2_small.png?12332456762384261517\" alt=\"Do not freeze\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"5x 1 mL Columns","offer_id":19098127684,"sku":"11-0230-5x1ML","price":172.0,"currency_code":"USD","in_stock":true},{"title":"5x 1 mL Columns with 1 Connector","offer_id":41405616259115,"sku":"11-0230-5x1MLB","price":200.0,"currency_code":"USD","in_stock":true},{"title":"1x 5 mL Column","offer_id":19098127748,"sku":"11-0230-1x5ML","price":149.0,"currency_code":"USD","in_stock":true},{"title":"1x 5 mL Column with 1 Connector","offer_id":41405616291883,"sku":"11-0230-1x5MLB","price":177.0,"currency_code":"USD","in_stock":true},{"title":"5x 5mL Columns","offer_id":19098127812,"sku":"11-0230-5x5ML","price":539.0,"currency_code":"USD","in_stock":true},{"title":"5x 5 mL Columns with 1 Connector","offer_id":41405616324651,"sku":"11-0230-5x5MLB","price":566.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/Ni-NTA_6FF_prepacked_columns.png?v=1496933949"},{"product_id":"proteindex-co-nta-agarose-6-ff","title":"PROTEINDEX™ PureBind™ Co-NTA Agarose 6 Fast Flow","description":"\u003cp\u003e\u003cstrong\u003ePROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e\u003c\/strong\u003e™\u003cstrong\u003e\u003cstrong\u003e\u003c\/strong\u003e Co-NTA Agarose 6FF\u003c\/strong\u003e is a  cobalt-based immobilized metal ion affinity chromatography (IMAC) medium. It offers greater specificity and therefore enhanced selectivity for polyhistidine-tagged proteins under both native and denaturing conditions, compared to Nickel-charged media. Co-NTA Agarose 6 Fast Flow resins can be used for small-scale and preparative large-scale purification of histidine-tagged recombinant proteins from prokaryotic and eukaryotic expression systems.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eProduct Highlights\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cstrong\u003eMechanical stable, suited for preparative and large scale purification of histidine-tagged proteins\u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cstrong\u003eHigher specificity and selectivity for His-tagged proteins, compared with Nickel-based IMAC. Can be used to purify challenging proteins that are expressed in low levels or when the use of nickel is not desired.\u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cstrong\u003eSuitable for purifying proteins from prokaryotic and eukaryotic expression systems\u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cstrong\u003eCompatible with applications under native and denaturing conditions \u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cstrong\u003eLow cobalt ion leakage.\u003c\/strong\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"200\"\u003e\u003cstrong\u003eLigand\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eCobalt ion\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBinding capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;20 mg 6XHis-tagged protein\/mL medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMatrix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eHighly cross-linked 6% agarose resins supplied as 50% slurry\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eParticle size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e45 μm - 165 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaximum pressure\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.3 MPa (3 bar)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eChemical compatibility\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003ctable width=\"93%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"37%\" valign=\"top\"\u003eReducing agents\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e10 mM β-mercaptoethanol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"37%\" valign=\"top\"\u003eDenaturing agents\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e8 M urea, 6 M Gua-HCl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"37%\" valign=\"top\"\u003eDetergents\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e\u0026lt;1% Triton™ X-100 (nonionic)\u003cbr\u003e1% NP-40 (nonionic)\u003cbr\u003e1% CHAPS (zwitterionic)\u003cbr\u003e1% SDS\u003cbr\u003e1% sarcosyl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"37%\" valign=\"top\"\u003eOther additives\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e≤500 mM imidazole at pH7.0 ~ 8.0 for elution\u003cbr\u003e30% ethanol\u003cbr\u003e20% glycerol\u003cbr\u003e500 mM KCl\u003cbr\u003e1.0 M NaCl\u003cbr\u003e20 mM MES\u003cbr\u003e50 mM Tris\u003cbr\u003e50 mM HEPES\u003cbr\u003e60 mM MOPS\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage buffer\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1X PBS containing 20% ethanol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProduct is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e   \u003cimg alt=\"Keep refrigerated\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Keep-refrigerated_95fa1867-1f0d-47e6-823a-8a0008076e21_small.png?12332456762384261517\" style=\"float: none;\"\u003e   \u003cimg alt=\"Do not freeze\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Do-not-freeze_2_small.png?12332456762384261517\" style=\"float: none;\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eDocuments\u003c\/h5\u003e\n\u003ch4\u003eProduct Information Sheet\u003c\/h4\u003e\n\u003cp\u003e🗎    \u003ca title=\"Man_11-0231_Co-NTA_Agarose_6_Fast_Flow_v1602.pdf\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Man_11-0231_Co-NTA_Agarose_6_Fast_Flow_v1602.pdf?3603577300140654506\" target=\"_blank\"\u003eCobalt NTA Agarose 6 Fast Flow\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"10 mL","offer_id":19152276164,"sku":"11-0231-010","price":126.0,"currency_code":"USD","in_stock":true},{"title":"50 mL","offer_id":19152276228,"sku":"11-0231-050","price":495.0,"currency_code":"USD","in_stock":true},{"title":"100 mL","offer_id":19152276356,"sku":"11-0231-100","price":874.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/11-0231_Co-NTA_6FF_842a62a0-6b68-40ab-89ca-5988b601f578.png?v=1483590410"},{"product_id":"proteindex-co-nta-agarose-6ff-prepacked-cartridge","title":"PROTEINDEX™ PureBind™ Co-NTA Agarose 6 Fast Flow, Prepacked Cartridge","description":"\u003cp\u003e\u003cstrong\u003eROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e\u003c\/strong\u003e™\u003cstrong\u003e\u003cstrong\u003e\u003c\/strong\u003e Co-NTA Agarose 6FF\u003c\/strong\u003e is a  cobalt-based immobilized metal ion affinity chromatography (IMAC) medium. It offers greater specificity and therefore enhanced selectivity for polyhistidine-tagged proteins under both native and denaturing conditions, compared to Nickel-charged media. Co-NTA Agarose 6 Fast Flow resins can be used for small-scale and preparative large-scale purification of histidine-tagged recombinant proteins from prokaryotic and eukaryotic expression systems.\u003c\/p\u003e\n\u003cp\u003eThe Prepacked Cartridge has standard interface and can be adapted to most commonly used chromatography systems, such as ÄKTA systems.\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eValue bundle with a \u003c\/span\u003e\u003ca rel=\"noopener\" title=\"Connector Luer Female \/ 10-32 Male in PEEK\" href=\"https:\/\/marvelgent.com\/products\/connector-1-16-luer-female-10-32-male-peek\" target=\"_blank\"\u003eLuer Female\/10-32 Male Connector in PEEK\u003c\/a\u003e\u003cspan\u003e (Cat# 20-0315) is now available for all pack sizes, providing convenient operation of the pre-packed column with a syringe at an excellent value.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eProduct Highlights\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cstrong\u003eReady-to-use, convenient formats: 1 mL and 5 mL\u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cstrong\u003eMechanical stable, suited for preparative and large scale purification of histidine-tagged proteins\u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cstrong\u003eHigher specificity and selectivity for His-tagged proteins, compared with Nickel-based IMAC. Can be used to purify challenging proteins that are expressed in low levels or when the use of nickel is not desired.\u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cstrong\u003eSuitable for purifying proteins from prokaryotic and eukaryotic expression systems\u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cstrong\u003eCompatible with applications under native and denaturing conditions \u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cstrong\u003eLow cobalt ion leakage.\u003c\/strong\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"200\"\u003e\u003cstrong\u003eLigand\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eCobalt ion\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBinding capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;20 mg 6XHis-tagged protein\/mL medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMatrix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eHighly cross-linked 6% agarose resins\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eParticle size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e45 μm - 165 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaximum pressure\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.3 MPa (3 bar)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eChemical compatibility\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003ctable width=\"93%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"37%\" valign=\"top\"\u003eReducing agents\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e10 mM β-mercaptoethanol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"37%\" valign=\"top\"\u003eDenaturing agents\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e8 M urea, 6 M Gua-HCl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"37%\" valign=\"top\"\u003eDetergents\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e\u0026lt;1% Triton™ X-100 (nonionic)\u003cbr\u003e1% NP-40 (nonionic)\u003cbr\u003e1% CHAPS (zwitterionic)\u003cbr\u003e1% SDS\u003cbr\u003e1% sarcosyl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"37%\" valign=\"top\"\u003eOther additives\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e≤500 mM imidazoleat pH7.0 ~ 8.0 for elution\u003cbr\u003e30% ethanol\u003cbr\u003e20% glycerol\u003cbr\u003e500 mM KCl\u003cbr\u003e1.0 M NaCl\u003cbr\u003e20 mM MES\u003cbr\u003e50 mM Tris\u003cbr\u003e50 mM HEPES\u003cbr\u003e60 mM MOPS\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage buffer\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1X PBS containing 20% ethanol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProduct is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e   \u003cimg alt=\"Keep refrigerated\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Keep-refrigerated_95fa1867-1f0d-47e6-823a-8a0008076e21_small.png?12332456762384261517\" style=\"float: none;\"\u003e   \u003cimg alt=\"Do not freeze\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Do-not-freeze_2_small.png?12332456762384261517\" style=\"float: none;\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"5x 1 mL Columns","offer_id":19255095236,"sku":"11-0253-5x1ML","price":181.0,"currency_code":"USD","in_stock":true},{"title":"5x 1 mL Columns with 1 Connector","offer_id":41405694279723,"sku":"11-0253-5x1MLB","price":209.0,"currency_code":"USD","in_stock":true},{"title":"1x 5 mL Column","offer_id":19255095300,"sku":"11-0253-1x5ML","price":152.0,"currency_code":"USD","in_stock":true},{"title":"1x 5 mL Column with 1 Connector","offer_id":41405694312491,"sku":"11-0253-1x5MLB","price":180.0,"currency_code":"USD","in_stock":true},{"title":"5x 5 mL Columns","offer_id":19255095364,"sku":"11-0253-5x5ML","price":592.0,"currency_code":"USD","in_stock":true},{"title":"5x 5 mL Columns with 1 Connector","offer_id":41405694345259,"sku":"11-0253-5x5MLB","price":620.0,"currency_code":"USD","in_stock":true},{"title":"16\/10 Column, 20 mL","offer_id":42061409845291,"sku":"11-0253-C20","price":629.0,"currency_code":"USD","in_stock":true},{"title":"7\/10 Columns, 4.7 mL, Pack of 2","offer_id":42061614022699,"sku":"11-0253-C47","price":478.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/Co-NTA_6FF_prepacked_columns.png?v=1483557966"},{"product_id":"empty-gravity-flow-columns-12-ml","title":"Empty Disposable Gravity Flow Columns, Fritted, 12 mL, with 20 µm frits","description":"\u003cp\u003eThese disposable columns are economical, versatile columns for single-use purification applications using gravity flow.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eSimple, cost-effective, readily adaptable for a wide range of gravity flow applications\u003c\/li\u003e\n\u003cli\u003eMade of high purity medical grade polypropylene, stable in commonly used chemicals and reagents\u003c\/li\u003e\n\u003cli\u003eAvailable in various sizes to suit for different experiments\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eIn the complete set, each fritted column comes with one top plug and one end cap. An extra filter disc is also provided and can be used as upper bed support. Placing a filter disc on top of the gravity-settled medium bed helps prevent drying of the medium.\u003c\/p\u003e\n\u003cp\u003eThe fritted column can be purchased separately as the SE version, providing a more cost-effective option for applications where no column caps nor extra frits are needed.\u003c\/p\u003e\n\u003ch4\u003e \u003c\/h4\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eProduct Details\u003c\/span\u003e\u003c\/h4\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"190\"\u003e\u003cstrong\u003eMaximum capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e12 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBed volume\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0 - 10 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eColumn inner diameter\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e15.6 mm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eColumn height\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e83 mm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaterial (column)\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003ePolypropylene (PP)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaterial (filter disc)\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003ePolyethylene (PE)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eFilter pore size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e20 µm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1 empty column\u003c\/p\u003e\n\u003cp\u003e1 top plug\u003c\/p\u003e\n\u003cp\u003e1 end cap\u003c\/p\u003e\n\u003cp\u003e2 filter discs (one is inserted and the other is provided as optional extra)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eChemical stability\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStable in all commonly used reagents\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"190\" valign=\"top\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFor fast, general-purpose purification by gravity flow or low pressure:\n\u003cul style=\"padding-left: 60px;\"\u003e\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003eAffinity chromatography of protein or other macromolecules\u003c\/li\u003e\n\u003cli\u003eImmunodepletion of immune reactive complexes\u003c\/li\u003e\n\u003cli\u003eSize-exclusion chromatography\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul style=\"padding-left: 60px;\"\u003e\u003c\/ul\u003e\n\u003cstrong\u003e\u003cem\u003eFor research use only. Not intended for any human or animal therapeutic or diagnostic uses.\u003c\/em\u003e\u003c\/strong\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eWe provide a variety of columns to fit your application needs. \u003ca href=\"https:\/\/marvelgent.com\/collections\/empty-disposable-gravity-flow-columns#top\" title=\"Empty Disposable Gravity Flow Columns\"\u003eView our collection and selection disposable gravity columns for your application \u0026gt;\u0026gt;\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"Pack of 50","offer_id":20503530244,"sku":"11-0257-050","price":147.0,"currency_code":"USD","in_stock":true},{"title":"Pack of 100","offer_id":20503530308,"sku":"11-0257-100","price":259.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/12_ml_disposable_columns_1.png?v=1543442209"},{"product_id":"empty-gravity-flow-columns-3-ml","title":"Empty Disposable Gravity Flow Columns, Fritted, 3 mL, 0.9 x 6 cm, with 20 µm frits","description":"\u003cp\u003ePolypropylene (PP) chromatography column, max. vol. 4 mL, cross-sectional area 0.64 cm\u003csup\u003e2\u003c\/sup\u003e, for use in low-pressure chromatography.\u003c\/p\u003e\n\u003cp\u003eThese disposable columns are economical, versatile columns for single-use purification applications using gravity flow.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eSimple, cost-effective, readily adaptable for a wide range of gravity flow applications\u003c\/li\u003e\n\u003cli\u003eMade of high purity medical grade polypropylene, stable in commonly used chemicals and reagents\u003c\/li\u003e\n\u003cli\u003eAvailable in various sizes to suit for different experiments\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eIn the complete set, each fritted column comes with one top plug and one end cap. An extra filter disc is also provided and can be used as upper bed support. Placing a filter disc on top of the gravity-settled medium bed helps prevent drying of the medium.\u003c\/p\u003e\n\u003cp\u003eThe fritted columns can be purchased separately as the SE version, providing a more cost-effective option for applications where no column caps nor extra frits are needed.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eProduct Details\u003c\/span\u003e\u003c\/h4\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"190\"\u003e\u003cstrong\u003eMaximum capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e4 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBed volume\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0 - 2.5 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eColumn inner diameter\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e8.9 mm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eColumn height\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e63 mm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaterial (column)\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003ePolypropylene (PP)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaterial (filter disc)\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003ePolyethylene (PE)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eFilter pore size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e20 µm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1 empty column\u003c\/p\u003e\n\u003cp\u003e1 top plug\u003c\/p\u003e\n\u003cp\u003e1 end cap\u003c\/p\u003e\n\u003cp\u003e2 filter discs (one is inserted and the other is provided as optional extra)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eChemical stability\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStable in all commonly used reagents\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFor fast, general-purpose purification by gravity flow or low pressure:\n\u003cul style=\"padding-left: 60px;\"\u003e\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003eAffinity chromatography of protein or other macromolecules\u003c\/li\u003e\n\u003cli\u003eImmunodepletion of immune reactive complexes\u003c\/li\u003e\n\u003cli\u003eSize-exclusion chromatography\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul style=\"padding-left: 60px;\"\u003e\u003c\/ul\u003e\n\u003cstrong\u003e\u003cem\u003eFor research use only. Not intended for any human or animal therapeutic or diagnostic uses.\u003c\/em\u003e\u003c\/strong\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eWe provide a variety of columns to fit your application needs. \u003ca href=\"https:\/\/marvelgent.com\/collections\/empty-disposable-gravity-flow-columns#top\" title=\"Empty Disposable Gravity Flow Columns\"\u003eView our collection and selection disposable gravity columns for your application \u0026gt;\u0026gt;\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"Pack of 50","offer_id":21332107460,"sku":"11-0258-050","price":107.0,"currency_code":"USD","in_stock":true},{"title":"Pack of 100","offer_id":21332107524,"sku":"11-0258-100","price":189.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/3_ml_disposable_columns.png?v=1565874864"},{"product_id":"empty-gravity-flow-columns-6-ml","title":"Empty Disposable Gravity Flow Columns, Fritted, 6 mL, with 20 µm frits","description":"\u003cp\u003eThese disposable columns are economical, versatile columns for single-use purification applications using gravity flow.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eSimple, cost-effective, readily adaptable for a wide range of gravity flow applications\u003c\/li\u003e\n\u003cli\u003eMade of high purity medical grade polypropylene, stable in commonly used chemicals and reagents\u003c\/li\u003e\n\u003cli\u003eAvailable in various sizes to suit for different experiments\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eIn the complete set, each fritted column comes with one top plug and one end cap. An extra filter disc is also provided and can be used as upper bed support. Placing a filter disc on top of the gravity-settled medium bed helps prevent drying of the medium.\u003c\/p\u003e\n\u003cp\u003eThe fritted column can be purchased separately as the SE version, providing a more cost-effective option for applications where no column caps nor extra frits are needed.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eProduct Details\u003c\/span\u003e\u003c\/h4\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"190\"\u003e\u003cstrong\u003eMaximum capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e6 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBed volume\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0 - 5 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eColumn inner diameter\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e12.7 mm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eColumn height\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e66 mm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaterial (column)\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003ePolypropylene (PP)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaterial (filter disc)\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003ePolyethylene (PE)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eFilter pore size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e20 µm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1 empty column\u003c\/p\u003e\n\u003cp\u003e1 top plug\u003c\/p\u003e\n\u003cp\u003e1 end cap\u003c\/p\u003e\n\u003cp\u003e2 filter discs (one is inserted and the other is provided as optional extra)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eChemical stability\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStable in all commonly used reagents\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFor fast, general-purpose purification by gravity flow or low pressure:\n\u003cul style=\"padding-left: 60px;\"\u003e\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003eAffinity chromatography of protein or other macromolecules\u003c\/li\u003e\n\u003cli\u003eImmunodepletion of immune reactive complexes\u003c\/li\u003e\n\u003cli\u003eSize-exclusion chromatography\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul style=\"padding-left: 60px;\"\u003e\u003c\/ul\u003e\n\u003cstrong\u003e\u003cem\u003eFor research use only. Not intended for any human or animal therapeutic or diagnostic uses.\u003c\/em\u003e\u003c\/strong\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eWe provide a variety of columns to fit your application needs. \u003ca href=\"https:\/\/marvelgent.com\/collections\/empty-disposable-gravity-flow-columns#top\" title=\"Empty Disposable Gravity Flow Columns\"\u003eView our collection and selection disposable gravity columns for your application \u0026gt;\u0026gt;\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"Pack of 50","offer_id":21332158020,"sku":"11-0259-050","price":124.0,"currency_code":"USD","in_stock":true},{"title":"Pack of 100","offer_id":21332158084,"sku":"11-0259-100","price":228.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/6_ml_disposable_columns-1.png?v=1565639998"},{"product_id":"empty-gravity-flow-columns-30-ml","title":"Empty Disposable Gravity Flow Columns, Fritted, 30 mL, with 20 µm frits","description":"\u003cp\u003eThese disposable columns are economical, versatile columns for single-use purification applications using gravity flow.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eSimple, cost-effective, readily adaptable for a wide range of gravity flow applications\u003c\/li\u003e\n\u003cli\u003eMade of high purity medical grade polypropylene, stable in commonly used chemicals and reagents\u003c\/li\u003e\n\u003cli\u003eAvailable in various sizes to suit for different experiments\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eEach empty column comes with one top plug and one end cap, as well as a filter disc as bed support. An extra filter disc is also provided and can be used as upper bed support. Placing a filter disc on top of the gravity-settled medium bed helps prevent drying of the medium.  \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eProduct Details\u003c\/span\u003e\u003c\/h4\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"200\"\u003e\u003cstrong\u003eMaximum capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e30 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBed volume\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0 - 25 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eColumn inner diameter\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e22.5 mm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eColumn height\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e101 mm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaterial (column)\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003ePolypropylene (PP)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaterial (filter disc)\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003ePolyethylene (PE)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eFilter pore size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e20 µm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1 empty column, with luer-lock outlet\u003c\/p\u003e\n\u003cp\u003e1 top plug\u003c\/p\u003e\n\u003cp\u003e1 end cap\u003c\/p\u003e\n\u003cp\u003e2 filter discs (one is inserted and the other is provided as optional extra)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eChemical stability\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStable in all commonly used reagents\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFor fast, general-purpose purification by gravity flow or low pressure:\n\u003cul style=\"padding-left: 60px;\"\u003e\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003eAffinity chromatography of protein or other macromolecules\u003c\/li\u003e\n\u003cli\u003eImmunodepletion of immune reactive complexes\u003c\/li\u003e\n\u003cli\u003eSize-exclusion chromatography\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul style=\"padding-left: 60px;\"\u003e\u003c\/ul\u003e\n\u003cstrong\u003e\u003cem\u003eFor research use only. Not intended for any human or animal therapeutic or diagnostic uses.\u003c\/em\u003e\u003c\/strong\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cbr\u003e\n\u003cp\u003eWe provide a variety of columns to fit your application needs. \u003ca href=\"https:\/\/marvelgent.com\/collections\/empty-disposable-gravity-flow-columns#top\" title=\"Empty Disposable Gravity Flow Columns\"\u003eView our collection and selection disposable gravity columns for your application \u0026gt;\u0026gt;\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"Pack of 25","offer_id":21332252228,"sku":"11-0260-025","price":183.0,"currency_code":"USD","in_stock":true},{"title":"Pack of 50","offer_id":40808355921963,"sku":"11-0260-050","price":341.0,"currency_code":"USD","in_stock":true},{"title":"Pack of 25, Bundled with Stopcocks","offer_id":40808353923115,"sku":"11-0260-025SC","price":260.0,"currency_code":"USD","in_stock":true},{"title":"Pack of 50, Bundled with Stopcocks","offer_id":21332252292,"sku":"11-0260-050SC","price":491.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/30_ml_disposable_columns.png?v=1565629687"},{"product_id":"empty-gravity-flow-columns-300-ml","title":"Empty Disposable Gravity Flow Columns, 300 mL with 20 µm frits","description":"\u003cp\u003eThese disposable columns are economical, versatile columns for single-use purification applications using gravity flow.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eSimple, cost-effective, readily adaptable for a wide range of gravity flow applications\u003c\/li\u003e\n\u003cli\u003eMade of high purity medical grade polypropylene, stable in commonly used chemicals and reagents\u003c\/li\u003e\n\u003cli\u003eAvailable in various sizes to suit for different experiments\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eEach empty column comes with a filter disc inserted as bed support. An extra filter disc is also provided for extra support and cleaning flow through when needed. It can also be used as upper bed support. Placing a filter disc on top of the gravity-settled medium bed helps prevent drying of the medium.  \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eProduct Details\u003c\/span\u003e\u003c\/h4\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"200\"\u003e\u003cstrong\u003eMaximum capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e300 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBed volume\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eup to 250 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eColumn inner diameter\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e49.4 mm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eColumn height\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e180 mm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaterial (column)\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003ePolypropylene (PP)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaterial (filter disc)\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUltrahigh molecular weight polyethylene (UHMW-PE), hydrophilic *\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaterial (stopcock)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003ePolycarbonates (PC) body, HDPE diverter\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eFilter pore size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e20 µm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1 empty column\u003c\/p\u003e\n\u003cp\u003e1 top plug\u003c\/p\u003e\n\u003cp\u003e1 end cap\u003c\/p\u003e\n\u003cp\u003e2 filter discs (one is inserted and the other is provided as optional extra)\u003c\/p\u003e\n\u003cp\u003e1-way stopcock, female luer to male luer (for Bundled with Stopcocks only)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eChemical stability\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStable in all commonly used reagents\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFor fast, general-purpose purification by gravity flow or low pressure:\n\u003cul style=\"padding-left: 60px;\"\u003e\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003eAffinity chromatography of protein or other macromolecules\u003c\/li\u003e\n\u003cli\u003eImmunodepletion of immune reactive complexes\u003c\/li\u003e\n\u003cli\u003eSize-exclusion chromatography\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003cul style=\"padding-left: 60px;\"\u003e\u003c\/ul\u003e\n\u003cstrong\u003e\u003cem\u003eFor research use only. Not intended for any human or animal therapeutic or diagnostic uses.\u003c\/em\u003e\u003c\/strong\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e* Hydrophobic version of filter discs is available upon request. \u003ca href=\"mailto:cust.support@marvelgent.com?subject=Request%20for%20300%20mL%20column%20with%2020%20um%20hydrophobic%20frits\"\u003eContact us\u003c\/a\u003e for more detail. \u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eWe provide a variety of columns to fit your application needs. \u003ca href=\"https:\/\/marvelgent.com\/collections\/empty-disposable-gravity-flow-columns#top\" title=\"Empty Disposable Gravity Flow Columns\"\u003eView our collection and selection disposable gravity columns for your application \u0026gt;\u0026gt;\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003e\u003c\/h5\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"Pack of 5","offer_id":11256366563371,"sku":"11-0261-005","price":158.0,"currency_code":"USD","in_stock":true},{"title":"Pack of 10","offer_id":17194076176427,"sku":"11-0261-010","price":275.0,"currency_code":"USD","in_stock":true},{"title":"Pack of 5, Bundled with Stopcocks","offer_id":17194055598123,"sku":"11-0261-005SC","price":184.0,"currency_code":"USD","in_stock":true},{"title":"Pack of 10, Bundled with Stopcocks","offer_id":21332288708,"sku":"11-0261-010SC","price":314.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/300_ml_disposable_column_2_d20750af-904d-4101-b50e-952a677a0367.png?v=1567534922"},{"product_id":"proteindex-iex-q-agarose-6-fast-flow","title":"PROTEINDEX™ IEX-Q Agarose 6 Fast Flow","description":"\u003cp\u003ePROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e™ Ion Exchange Media CM, SP, DEAE, Q Agarose 6FF are specifically developed for high-resolution separation of proteins, peptides, nucleic acids and other charged biomolecules according to their difference in surface charge. The base matrix of PROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e™ IEX-CM, IEX-SP, IEX-DEAE, IEX-Q is highly cross-linked 6% agarose, providing excellent chemical and physical stability.\u003c\/p\u003e\n\u003cp\u003eThe type of charged group determines the type and strength of the exchanger, while the total number and availability of the charged groups determine the capacity. Sulfonic and quaternary amines form strong ion exchangers, which are completely ionized over a broad pH range. Others form weak ion exchangers, where the degree of dissociation, and thus the exchange capacity, varies markedly with pH. \"Strong\" and \"weak\" refer to the extent of ionization with pH, and not to the strength of binding.\u003c\/p\u003e\n\u003cp\u003eMarvelgent ion exchanger media are optimized for high sample loading capacity with reliable, reproducible performance, suited for process scale chromatography. \u003ca title=\"Marvelgent Ion Exchange Chromatography IEX Media\" href=\"http:\/\/marvelgent.com\/collections\/ion-exchange-chromatography-iex\"\u003eView all IEX media.\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e \u003c\/p\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"200\"\u003e\u003cstrong\u003eIon exchange type\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStrong anion\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTotal ionic capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.18 – 0.25 mmol Cl\u003csup\u003e–\u003c\/sup\u003e\/mL medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMatrix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eHighly cross-linked 6% agarose supplied as a 50% slurry\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eParticle size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e45 μm - 165 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eFlow rate\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e400-700 cm\/h\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003epH stability\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003epH 2 - 12\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2 - 30°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage buffer\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e20% ethanol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProduct is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e   \u003cimg alt=\"Keep refrigerated\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Keep-refrigerated_95fa1867-1f0d-47e6-823a-8a0008076e21_small.png?12332456762384261517\" style=\"float: none;\"\u003e   \u003cimg alt=\"Do not freeze\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Do-not-freeze_2_small.png?12332456762384261517\" style=\"float: none;\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eDocuments\u003c\/h5\u003e\n\u003ch4\u003eProduct Information Sheet\u003c\/h4\u003e\n\u003cp\u003e🗎   \u003ca title=\"Manu - Ion exchange media CM, SP, DEAE, Q agarose 6FF resins\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Man__IEX-CM_IEX-SP_IEX-DEAE_IEX-Q_Agarose_6_FF_v1602.pdf?6113480773629873678\" rel=\"noopener noreferrer\" target=\"_blank\"\u003eProteIndex™ IEX-CM, IEX-SP, IEX-DEAE, IEX-Q Agarose 6 Fast Flow\u003c\/a\u003e\u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"25 mL","offer_id":25417343816,"sku":"11-0232-025","price":77.0,"currency_code":"USD","in_stock":true},{"title":"500 mL","offer_id":15651682418731,"sku":"11-0232-500","price":764.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/11-0232_IEX-Q_6FF.png?v=1493135988"},{"product_id":"proteindex-iex-sp-agarose-6-fast-flow","title":"PROTEINDEX™ IEX-SP Agarose 6 Fast Flow","description":"\u003cp\u003ePROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e™ Ion Exchange Media CM, SP, DEAE, Q Agarose 6FF are specifically developed for high-resolution separation of proteins, peptides, nucleic acids and other charged biomolecules according to their difference in surface charge. The base matrix of PROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e™ IEX-CM, IEX-SP, IEX-DEAE, IEX-Q is highly cross-linked 6% agarose, providing excellent chemical and physical stability.\u003c\/p\u003e\n\u003cp\u003eThe type of charged group determines the type and strength of the exchanger, while the total number and availability of the charged groups determine the capacity. Sulfonic and quaternary amines form strong ion exchangers, which are completely ionized over a broad pH range. Others form weak ion exchangers, where the degree of dissociation, and thus the exchange capacity, varies markedly with pH. \"Strong\" and \"weak\" refer to the extent of ionization with pH, and not to the strength of binding.\u003c\/p\u003e\n\u003cp\u003eMarvelgent ion exchanger media are optimized for high sample loading capacity with reliable, reproducible performance, suited for process scale chromatography. \u003ca title=\"Marvelgent Ion Exchange Chromatography IEX Media\" href=\"http:\/\/marvelgent.com\/collections\/ion-exchange-chromatography-iex\"\u003eView all IEX media.\u003c\/a\u003e\u003c\/p\u003e\n\u003cbr\u003e \n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"200\"\u003e\u003cstrong\u003eIon exchange type\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStrong cation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTotal ionic capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.18 – 0.25 mmol H\u003csup\u003e+\u003c\/sup\u003e\/mL medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMatrix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eHighly cross-linked 6% agarose supplied as a 50% slurry\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eParticle size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e45 μm - 165 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eFlow rate\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e400-700 cm\/h\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003epH stability\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003epH 4 - 13\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2 - 30°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage buffer\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e20% ethanol, 0.2 M Sodium acetate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProduct is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e   \u003cimg alt=\"Keep refrigerated\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Keep-refrigerated_95fa1867-1f0d-47e6-823a-8a0008076e21_small.png?12332456762384261517\" style=\"float: none;\"\u003e   \u003cimg alt=\"Do not freeze\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Do-not-freeze_2_small.png?12332456762384261517\" style=\"float: none;\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eDocuments\u003c\/h5\u003e\n\u003ch4\u003eProduct Information Sheet\u003c\/h4\u003e\n\u003cp\u003e🗎   \u003ca target=\"_blank\" title=\"Manu - Ion exchange media CM, SP, DEAE, Q agarose 6FF resins\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Man__IEX-CM_IEX-SP_IEX-DEAE_IEX-Q_Agarose_6_FF_v1602.pdf?6113480773629873678\"\u003eProteIndex™ IEX-CM, IEX-SP, IEX-DEAE, IEX-Q Agarose 6 Fast Flow\u003c\/a\u003e\u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"25 mL","offer_id":25422177992,"sku":"11-0234-025","price":77.0,"currency_code":"USD","in_stock":true},{"title":"500 mL","offer_id":15651751395371,"sku":"11-0234-500","price":764.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/11-0234_IEX-SP.png?v=1493136549"},{"product_id":"proteindex-iex-deae-agarose-6-fast-flow","title":"PROTEINDEX™ IEX-DEAE Agarose 6 Fast Flow","description":"\u003cp\u003ePROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e™ Ion Exchange Media CM, SP, DEAE, Q Agarose 6FF are specifically developed for high-resolution separation of proteins, peptides, nucleic acids and other charged biomolecules according to their difference in surface charge. The base matrix of PROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e™ IEX-CM, IEX-SP, IEX-DEAE, IEX-Q is highly cross-linked 6% agarose, providing excellent chemical and physical stability.\u003c\/p\u003e\n\u003cp\u003eThe type of charged group determines the type and strength of the exchanger, while the total number and availability of the charged groups determine the capacity. Sulfonic and quaternary amines form strong ion exchangers, which are completely ionized over a broad pH range. Others form weak ion exchangers, where the degree of dissociation, and thus the exchange capacity, varies markedly with pH. \"Strong\" and \"weak\" refer to the extent of ionization with pH, and not to the strength of binding.\u003c\/p\u003e\n\u003cp\u003eMarvelgent ion exchanger media are optimized for high sample loading capacity with reliable, reproducible performance, suited for process scale chromatography. \u003ca title=\"Marvelgent Ion Exchange Chromatography IEX Media\" href=\"http:\/\/marvelgent.com\/collections\/ion-exchange-chromatography-iex\"\u003eView all IEX media.\u003c\/a\u003e\u003c\/p\u003e\n\u003cbr\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"200\"\u003e\u003cstrong\u003eIon exchange type\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eWeak anion\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTotal ionic capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.11 – 0.16 mmol Cl\u003csup\u003e–\u003c\/sup\u003e\/mL medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMatrix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eHighly cross-linked 6% agarose supplied as a 50% slurry\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eParticle size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e45 μm - 165 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eFlow rate\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e300 - 600 cm\/h\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003epH stability\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003epH 2 - 12\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2 - 30°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage buffer\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e20% ethanol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProduct is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e   \u003cimg alt=\"Keep refrigerated\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Keep-refrigerated_95fa1867-1f0d-47e6-823a-8a0008076e21_small.png?12332456762384261517\" style=\"float: none;\"\u003e   \u003cimg alt=\"Do not freeze\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Do-not-freeze_2_small.png?12332456762384261517\" style=\"float: none;\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eDocuments\u003c\/h5\u003e\n\u003ch4\u003eProduct Information Sheet\u003c\/h4\u003e\n\u003cp\u003e🗎   \u003ca target=\"_blank\" title=\"Manu - Ion exchange media CM, SP, DEAE, Q agarose 6FF resins\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Man__IEX-CM_IEX-SP_IEX-DEAE_IEX-Q_Agarose_6_FF_v1602.pdf?6113480773629873678\"\u003eProteIndex™ IEX-CM, IEX-SP, IEX-DEAE, IEX-Q Agarose 6 Fast Flow\u003c\/a\u003e\u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"25 mL","offer_id":25422252936,"sku":"11-0236-025","price":77.0,"currency_code":"USD","in_stock":true},{"title":"500 mL","offer_id":15651740254251,"sku":"11-0236-500","price":764.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/11-0236_IEX-DEAE_6FF.png?v=1493133246"},{"product_id":"proteindex-iex-cm-agarose-6-fast-flow","title":"PROTEINDEX™ IEX-CM Agarose 6 Fast Flow","description":"\u003cp\u003ePROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e™ Ion Exchange Media CM, SP, DEAE, Q Agarose 6FF are specifically developed for high-resolution separation of proteins, peptides, nucleic acids and other charged biomolecules according to their difference in surface charge. The base matrix of PROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e™ IEX-CM, IEX-SP, IEX-DEAE, IEX-Q Agarose 6 Fast Flow is highly 6% cross-linked agarose, providing excellent chemical and physical stability.\u003c\/p\u003e\n\u003cp\u003eThe type of charged group determines the type and strength of the exchanger, while the total number and availability of the charged groups determine the capacity. Sulfonic and quaternary amines form strong ion exchangers, which are completely ionized over a broad pH range. Others form weak ion exchangers, where the degree of dissociation, and thus the exchange capacity, varies markedly with pH. \"Strong\" and \"weak\" refer to the extent of ionization with pH, and not to the strength of binding.\u003c\/p\u003e\n\u003cp\u003eMarvelgent ion exchanger media are optimized for high sample loading capacity with reliable, reproducible performance, suited for process scale chromatography. \u003ca title=\"Marvelgent Ion Exchange Chromatography IEX Media\" href=\"http:\/\/marvelgent.com\/collections\/ion-exchange-chromatography-iex\"\u003eView all IEX media.\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px;\" width=\"200\"\u003e\u003cstrong\u003eIon exchange type\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 22px;\"\u003eWeak cation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px;\"\u003e\u003cstrong\u003eTotal ionic capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 22px;\"\u003e0.09 - 0.13 mmol H\u003csup\u003e+ \u003c\/sup\u003e\/mL medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px;\"\u003e\u003cstrong\u003eMatrix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 22px;\"\u003eHighly cross-linked 6% agarose supplied as a 50% slurry\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px;\"\u003e\u003cstrong\u003eParticle size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 22px;\"\u003e45 μm - 165 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px;\"\u003e\u003cstrong\u003eFlow rate\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 22px;\"\u003e300-600 cm\/h\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22.2333px;\"\u003e\n\u003ctd style=\"height: 22.2333px;\" valign=\"top\"\u003e\u003cstrong\u003epH stability\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 22.2333px;\"\u003epH 4 - 13\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px;\"\u003e\u003cstrong\u003eStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 22px;\"\u003e2 - 30°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px;\"\u003e\u003cstrong\u003eStorage buffer\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 22px;\"\u003e20% ethanol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProduct is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e   \u003cimg alt=\"Keep refrigerated\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Keep-refrigerated_95fa1867-1f0d-47e6-823a-8a0008076e21_small.png?12332456762384261517\" style=\"float: none;\"\u003e   \u003cimg alt=\"Do not freeze\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Do-not-freeze_2_small.png?12332456762384261517\" style=\"float: none;\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eDocuments\u003c\/h5\u003e\n\u003ch4\u003eProduct Information Sheet\u003c\/h4\u003e\n\u003cp\u003e🗎   \u003ca target=\"_blank\" title=\"Manu - Ion exchange media CM, SP, DEAE, Q agarose 6FF resins\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Man__IEX-CM_IEX-SP_IEX-DEAE_IEX-Q_Agarose_6_FF_v1602.pdf?6113480773629873678\" rel=\"noopener noreferrer\"\u003eProteIndex™ IEX-CM, IEX-SP, IEX-DEAE, IEX-Q Agarose 6 Fast Flow\u003c\/a\u003e\u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"25 mL","offer_id":25422712200,"sku":"11-0238-025","price":77.0,"currency_code":"USD","in_stock":true},{"title":"500 mL","offer_id":15651721478187,"sku":"11-0238-500","price":764.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/11-0238_IEX-CM_6FF.png?v=1493135347"},{"product_id":"proteindex-hic-butyl-agarose-4-fast-flow","title":"PROTEINDEX™ HIC-Butyl Agarose 4 Fast Flow","description":"\u003cp\u003ePROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e™ Hydrophobic Interaction Chromatography media Butyl Agarose 4 Fast Flow, Octyl Agarose 4 Fast Flow, Phenyl Agarose 6 Fast Flow (Low Phenyl) and Phenyl Agarose 6 Fast Flow (High Phenyl) are optimized for high throughput, high-resolution, high-capacity separation of proteins and other biomolecules based on their hydrophobicity.\u003c\/p\u003e\n\u003cp\u003eDynamic binding capacity of a HIC medium depends on factors such as protein properties, salt type and concentration, presence of detergents, flow rate, temperature, and pH, but also on the properties of the medium. The base matrix of Marvelgent ProteIndex™ is highly cross-linked agarose, providing high efficiency and super physical and chemical stability for both lab scale and processing scale applications. \u003ca title=\"Marvelgent Hydrophobic Interaction Chromatography HIC Media\" href=\"http:\/\/marvelgent.com\/collections\/hydrophobic-interaction-chromatography-media\"\u003eView all HIC media.\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px;\" width=\"200\"\u003e\u003cstrong\u003eLigand\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 22px;\"\u003eButyl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px;\" width=\"200\"\u003e\u003cstrong\u003eMatrix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 22px;\"\u003eHighly cross-linked 4% agarose supplied as a 50% slurry\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px;\"\u003e\u003cstrong\u003eCapacity (per mL medium)\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 22px;\"\u003e7 mg IgG; 26 mg BSA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px;\"\u003e\u003cstrong\u003eParticle size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 22px;\"\u003e45 μm - 165 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px;\"\u003e\u003cstrong\u003eFlow rate\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 22px;\"\u003e≥150 cm\/h\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22.2333px;\"\u003e\n\u003ctd style=\"height: 22.2333px;\" valign=\"top\"\u003e\u003cstrong\u003epH stability\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 22.2333px;\"\u003epH 3 - 13\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px;\"\u003e\u003cstrong\u003eStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 22px;\"\u003e2 - 30°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px;\"\u003e\u003cstrong\u003eStorage buffer\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 22px;\"\u003e20% ethanol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProduct is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e   \u003cimg alt=\"Keep refrigerated\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Keep-refrigerated_95fa1867-1f0d-47e6-823a-8a0008076e21_small.png?12332456762384261517\" style=\"float: none;\"\u003e   \u003cimg alt=\"Do not freeze\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Do-not-freeze_2_small.png?12332456762384261517\" style=\"float: none;\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eDocuments\u003c\/h5\u003e\n\u003ch4\u003eProduct Information Sheet\u003c\/h4\u003e\n\u003cp\u003e🗎   \u003ca title=\"Manu - Hydrophobic Interaction Chromatography Butyl, Octyl, Phenyl Media\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Man_Hydrophobic_Interaction_Chromatography_v1602.pdf?15068444023549913865\" rel=\"noopener noreferrer\" target=\"_blank\"\u003eProteIndex™ Hydrophobic Interaction Chromatography Butyl, Octyl, Phenyl Media\u003c\/a\u003e\u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"25 mL","offer_id":25434738632,"sku":"11-0240-025","price":152.0,"currency_code":"USD","in_stock":true},{"title":"200 mL","offer_id":25434738696,"sku":"11-0240-200","price":756.0,"currency_code":"USD","in_stock":true},{"title":"500 mL","offer_id":15651923427371,"sku":"11-0240-500","price":1588.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/Marvelgent_Biosciences_products_3_857ef980-2c89-4fb4-a94a-4ebc4903a692.png?v=1474303750"},{"product_id":"proteindex-hic-octyl-agarose-4-fast-flow","title":"PROTEINDEX™ HIC-Octyl Agarose 4 Fast Flow","description":"\u003cp\u003ePROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e™ Hydrophobic Interaction Chromatography media Butyl Agarose 4 Fast Flow, Octyl Agarose 4 Fast Flow, Phenyl Agarose 6 Fast Flow (Low Phenyl) and Phenyl Agarose 6 Fast Flow (High Phenyl) are optimized for high throughput, high-resolution, high-capacity separation of proteins and other biomolecules based on their hydrophobicity.\u003c\/p\u003e\n\u003cp\u003eDynamic binding capacity of a HIC medium depends on factors such as protein properties, salt type and concentration, presence of detergents, flow rate, temperature, and pH, but also on the properties of the medium. The base matrix of Marvelgent ProteIndex™ is highly cross-linked agarose, providing high efficiency and super physical and chemical stability for both lab scale and processing scale applications. \u003ca title=\"Marvelgent Hydrophobic Interaction Chromatography HIC Media\" href=\"http:\/\/marvelgent.com\/collections\/hydrophobic-interaction-chromatography-media\"\u003eView all HIC media.\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px;\" width=\"200\"\u003e\u003cstrong\u003eLigand\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 22px;\"\u003eOctyl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px;\" width=\"200\"\u003e\u003cstrong\u003eMatrix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 22px;\"\u003eHighly cross-linked 4% agarose supplied as a 50% slurry\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px;\"\u003e\u003cstrong\u003eCapacity (per mL medium)\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 22px;\"\u003e26 mg IgG; 7 mg BSA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px;\"\u003e\u003cstrong\u003eParticle size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 22px;\"\u003e45 μm - 165 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px;\"\u003e\u003cstrong\u003eFlow rate\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 22px;\"\u003e≥150 cm\/h\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22.2333px;\"\u003e\n\u003ctd style=\"height: 22.2333px;\" valign=\"top\"\u003e\u003cstrong\u003epH stability\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 22.2333px;\"\u003epH 3 - 13\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px;\"\u003e\u003cstrong\u003eStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 22px;\"\u003e2 - 30°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px;\"\u003e\u003cstrong\u003eStorage buffer\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 22px;\"\u003e20% ethanol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProduct is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e   \u003cimg alt=\"Keep refrigerated\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Keep-refrigerated_95fa1867-1f0d-47e6-823a-8a0008076e21_small.png?12332456762384261517\" style=\"float: none;\"\u003e   \u003cimg alt=\"Do not freeze\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Do-not-freeze_2_small.png?12332456762384261517\" style=\"float: none;\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eDocuments\u003c\/h5\u003e\n\u003ch4\u003eProduct Information Sheet\u003c\/h4\u003e\n\u003cp\u003e🗎   \u003ca title=\"Manu - Hydrophobic Interaction Chromatography Butyl, Octyl, Phenyl Media\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Man_Hydrophobic_Interaction_Chromatography_v1602.pdf?15068444023549913865\" rel=\"noopener noreferrer\" target=\"_blank\"\u003eProteIndex™ Hydrophobic Interaction Chromatography Butyl, Octyl, Phenyl Media\u003c\/a\u003e\u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"25 mL","offer_id":25436381640,"sku":"11-0242-025","price":152.0,"currency_code":"USD","in_stock":true},{"title":"200 mL","offer_id":25436381704,"sku":"11-0242-200","price":756.0,"currency_code":"USD","in_stock":true},{"title":"500 mL","offer_id":15651941941291,"sku":"11-0242-500","price":1588.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/Marvelgent_Biosciences_products_3_9bf8fc21-3c61-4716-a812-0d56d6017126.png?v=1474308489"},{"product_id":"proteindex-hic-phenyl-agarose-4-fast-flow-low-phenyl","title":"PROTEINDEX™ HIC-Phenyl Agarose 6 Fast Flow,  Low Phenyl","description":"\u003cp\u003ePROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e™ Hydrophobic Interaction Chromatography media Butyl Agarose 4 Fast Flow, Octyl Agarose 4 Fast Flow, Phenyl Agarose 6 Fast Flow (Low Phenyl) and Phenyl Agarose 6 Fast Flow (High Phenyl) are optimized for high throughput, high-resolution, high-capacity separation of proteins and other biomolecules based on their hydrophobicity.\u003c\/p\u003e\n\u003cp\u003eDynamic binding capacity of a HIC medium depends on factors such as protein properties, salt type and concentration, presence of detergents, flow rate, temperature, and pH, but also on the properties of the medium. The base matrix of Marvelgent ProteIndex™ is highly cross-linked agarose, providing high efficiency and super physical and chemical stability for both lab scale and processing scale applications. \u003ca title=\"Marvelgent Hydrophobic Interaction Chromatography HIC Media\" href=\"http:\/\/marvelgent.com\/collections\/hydrophobic-interaction-chromatography-media\"\u003eView all HIC media.\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px;\" width=\"200\"\u003e\u003cstrong\u003eLigand\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 22px;\"\u003ePhenyl, ~25 μmol\/mL medium \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px;\" width=\"200\"\u003e\u003cstrong\u003eMatrix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 22px;\"\u003eHighly cross-linked 6% agarose supplied as a 50% slurry\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px;\"\u003e\u003cstrong\u003eCapacity (per mL medium)\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 22px;\"\u003e10 mg IgG; 24 mg BSA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px;\"\u003e\u003cstrong\u003eParticle size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 22px;\"\u003e45 μm - 165 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px;\"\u003e\u003cstrong\u003eFlow rate\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 22px;\"\u003e300 – 600 cm\/h\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22.2333px;\"\u003e\n\u003ctd style=\"height: 22.2333px;\" valign=\"top\"\u003e\u003cstrong\u003epH stability\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 22.2333px;\"\u003epH 3 - 13\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px;\"\u003e\u003cstrong\u003eStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 22px;\"\u003e2 - 30°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px;\"\u003e\u003cstrong\u003eStorage buffer\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 22px;\"\u003e20% ethanol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProduct is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e   \u003cimg alt=\"Keep refrigerated\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Keep-refrigerated_95fa1867-1f0d-47e6-823a-8a0008076e21_small.png?12332456762384261517\" style=\"float: none;\"\u003e   \u003cimg alt=\"Do not freeze\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Do-not-freeze_2_small.png?12332456762384261517\" style=\"float: none;\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eDocuments\u003c\/h5\u003e\n\u003ch4\u003eProduct Information Sheet\u003c\/h4\u003e\n\u003cp\u003e🗎   \u003ca title=\"Manu - Hydrophobic Interaction Chromatography Butyl, Octyl, Phenyl Media\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Man_Hydrophobic_Interaction_Chromatography_v1602.pdf?15068444023549913865\" rel=\"noopener noreferrer\" target=\"_blank\"\u003eProteIndex™ Hydrophobic Interaction Chromatography Butyl, Octyl, Phenyl Media\u003c\/a\u003e\u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"25 mL","offer_id":25436462728,"sku":"11-0244-025","price":152.0,"currency_code":"USD","in_stock":true},{"title":"200 mL","offer_id":25436462792,"sku":"11-0244-200","price":756.0,"currency_code":"USD","in_stock":true},{"title":"500 mL","offer_id":15651964715051,"sku":"11-0244-500","price":1588.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/Marvelgent_Biosciences_products_3_486cef5e-cc44-46ba-bc58-28571b60251c.png?v=1474308719"},{"product_id":"proteindex-hic-phenyl-agarose-6-fast-flow-high-phenyl","title":"PROTEINDEX™ HIC-Phenyl Agarose 6 Fast Flow,  High Phenyl","description":"\u003cp\u003ePROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e™ Hydrophobic Interaction Chromatography media Butyl Agarose 4 Fast Flow, Octyl Agarose 4 Fast Flow, Phenyl Agarose 6 Fast Flow (Low Phenyl) and Phenyl Agarose 6 Fast Flow (High Phenyl) are optimized for high throughput, high-resolution, high-capacity separation of proteins and other biomolecules based on their hydrophobicity.\u003c\/p\u003e\n\u003cp\u003eDynamic binding capacity of a HIC medium depends on factors such as protein properties, salt type and concentration, presence of detergents, flow rate, temperature, and pH, but also on the properties of the medium. The base matrix of Marvelgent ProteIndex™ is highly cross-linked agarose, providing high efficiency and super physical and chemical stability for both lab scale and processing scale applications. \u003ca title=\"Marvelgent Hydrophobic Interaction Chromatography HIC Media\" href=\"http:\/\/marvelgent.com\/collections\/hydrophobic-interaction-chromatography-media\"\u003eView all HIC media.\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px;\" width=\"200\"\u003e\u003cstrong\u003eLigand\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 22px;\"\u003ePhenyl, ~40 μmol\/mL medium \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px;\" width=\"200\"\u003e\u003cstrong\u003eMatrix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 22px;\"\u003eHighly cross-linked 6% agarose supplied as a 50% slurry\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px;\"\u003e\u003cstrong\u003eCapacity (per mL medium)\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 22px;\"\u003e30 mg IgG; 36 mg BSA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px;\"\u003e\u003cstrong\u003eParticle size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 22px;\"\u003e45 μm - 165 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px;\"\u003e\u003cstrong\u003eFlow rate\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 22px;\"\u003e300 – 600 cm\/h\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22.2333px;\"\u003e\n\u003ctd style=\"height: 22.2333px;\" valign=\"top\"\u003e\u003cstrong\u003epH stability\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 22.2333px;\"\u003epH 3 - 13\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px;\"\u003e\u003cstrong\u003eStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 22px;\"\u003e2 - 30°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"height: 22px;\"\u003e\u003cstrong\u003eStorage buffer\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 22px;\"\u003e20% ethanol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProduct is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e   \u003cimg alt=\"Keep refrigerated\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Keep-refrigerated_95fa1867-1f0d-47e6-823a-8a0008076e21_small.png?12332456762384261517\" style=\"float: none;\"\u003e   \u003cimg alt=\"Do not freeze\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Do-not-freeze_2_small.png?12332456762384261517\" style=\"float: none;\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eDocuments\u003c\/h5\u003e\n\u003ch4\u003eProduct Information Sheet\u003c\/h4\u003e\n\u003cp\u003e🗎   \u003ca title=\"Manu - Hydrophobic Interaction Chromatography Butyl, Octyl, Phenyl Media\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Man_Hydrophobic_Interaction_Chromatography_v1602.pdf?15068444023549913865\" rel=\"noopener noreferrer\" target=\"_blank\"\u003eProteIndex™ Hydrophobic Interaction Chromatography Butyl, Octyl, Phenyl Media\u003c\/a\u003e\u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"25 mL","offer_id":25436555336,"sku":"11-0246-025","price":152.0,"currency_code":"USD","in_stock":true},{"title":"200 mL","offer_id":25436555400,"sku":"11-0246-200","price":756.0,"currency_code":"USD","in_stock":true},{"title":"500 mL","offer_id":15651954786347,"sku":"11-0246-500","price":1588.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/Marvelgent_Biosciences_products_3_507dfa1b-a2b9-4304-89be-e4febef7e053.png?v=1474309154"},{"product_id":"proteindex-co-nta-agarose","title":"PROTEINDEX™ PureBind™ Co-NTA Agarose","description":"\u003cp\u003e\u003cstrong\u003ePROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e\u003c\/strong\u003e™\u003cstrong\u003e\u003cstrong\u003e\u003c\/strong\u003e PureBind™ Co-NTA Agarose \u003c\/strong\u003eis a  cobalt-based immobilized metal ion affinity chromatography (IMAC) medium. It offers greater specificity and therefore enhanced selectivity for polyhistidine-tagged proteins under both native and denaturing conditions, compared to Ni-NTA media.     \u003c\/p\u003e\n\u003cp\u003eCo-NTA Agarose resins can be used for batch\/gravity flow purification of polyhistidine-tagged recombinant proteins.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eProduct Highlights\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cstrong\u003eSuited for batch or gravity flow purification of histidine-tagged proteins\u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cstrong\u003eHigher specificity and selectivity for His-tagged proteins, compared with Ni-NTA IMAC. Can be used to purify challenging proteins that are expressed in low levels or when the use of nickel is not desired. \u003cbr\u003e\u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cstrong\u003eSuitable for purifying proteins from prokaryotic and eukaryotic expression systems\u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cstrong\u003eCompatible with applications under native and denaturing conditions \u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cstrong\u003eLow cobalt ion leakage.\u003c\/strong\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"200\"\u003e\u003cstrong\u003eLigand\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eCobalt ion\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBinding capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;20 mg 6XHis-tagged protein\/mL medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMatrix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e4% agarose resins supplied as 50% slurry\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eParticle size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e45 μm - 165 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaximum pressure\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.1 MPa (1 bar)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eChemical compatibility\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003ctable width=\"93%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"37%\"\u003eReducing agents\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e10 mM β-mercaptoethanol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"37%\"\u003eDenaturing agents\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e8 M urea, 6 M Gua-HCl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"37%\"\u003eDetergents\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e\u0026lt;1% Triton™ X-100 (nonionic)\u003cbr\u003e1% NP-40 (nonionic)\u003cbr\u003e1% CHAPS (zwitterionic)\u003cbr\u003e1% SDS\u003cbr\u003e1% sarcosyl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"37%\"\u003eOther additives\u003c\/td\u003e\n\u003ctd width=\"68%\"\u003e≤500 mM imidazole at pH7.0 ~ 8.0 for elution\u003cbr\u003e30% ethanol\u003cbr\u003e20% glycerol\u003cbr\u003e500 mM KCl\u003cbr\u003e1.0 M NaCl\u003cbr\u003e20 mM MES\u003cbr\u003e50 mM Tris\u003cbr\u003e50 mM HEPES\u003cbr\u003e60 mM MOPS\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage buffer\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1X PBS containing 20% ethanol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProduct is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e   \u003cimg style=\"float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Keep-refrigerated_95fa1867-1f0d-47e6-823a-8a0008076e21_small.png?12332456762384261517\" alt=\"Keep refrigerated\"\u003e   \u003cimg style=\"float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Do-not-freeze_2_small.png?12332456762384261517\" alt=\"Do not freeze\"\u003e\u003c\/p\u003e\n\u003ch5\u003e\u003cbr\u003e\u003c\/h5\u003e\n\u003ch5\u003eDocuments\u003c\/h5\u003e\n\u003ch4\u003eProduct Information Sheet\u003c\/h4\u003e\n\u003cp\u003e🗎    \u003ca rel=\"noopener noreferrer\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Man_11-0252_Co-NTA_Agarose_v1605.pdf?16279790217106739219\" title=\"Man_11-0252_Co-NTA_Agarose_v1605.pdf\" target=\"_blank\"\u003eCobalt NTA Agarose \u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003e\u003cbr\u003e\u003c\/h5\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"10 mL","offer_id":26309510600,"sku":"11-0252-010","price":121.0,"currency_code":"USD","in_stock":true},{"title":"50 mL","offer_id":26309510728,"sku":"11-0252-050","price":498.0,"currency_code":"USD","in_stock":true},{"title":"100 mL","offer_id":26309510792,"sku":"11-0252-100","price":874.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/11-0252_Co-NTA.png?v=1483557922"},{"product_id":"proteindex-ni-ida-agarose-6ff","title":"PROTEINDEX™ Ni-IDA Agarose 6FF","description":"\u003cp\u003e\u003cstrong\u003ePROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e\u003c\/strong\u003e™ \u003cstrong\u003eNi-IDA Agarose\u003c\/strong\u003e \u003cstrong\u003e6FF\u003c\/strong\u003e is designed mainly for scaling up purification of histidine-tagged proteins that are expressed in various hosts, such as \u003cem\u003eE.coli\u003c\/em\u003e, yeast, insect cells, and mammalian cells.\u003c\/p\u003e\n\u003cp\u003eHighly cross-linked 6% agarose as the base matrix support offers excellent mechanical stability for centrifugation, and automated, scale up purification of histidine-tagged proteins.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eProduct Highlights\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cstrong\u003eMechanical stable, designed for high yield, scaling up purification of histidine-tagged proteins\u003cbr\u003e\u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cstrong\u003eHigher binding capacity\u003cbr\u003e\u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cstrong\u003eLow leakage of nickel ions\u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cstrong\u003eVersatile, ready for gravity\/batch, spin, or FPLC \u003cspan data-mce-fragment=\"1\"\u003esystems\u003c\/span\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"200\"\u003e\u003cstrong\u003eLigand\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eNickel\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBinding capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;40 mg 6xHis-tagged protein\/mL medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMatrix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e6% highly cross-linked agarose supplied as 50% slurry\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eParticle size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e45 μm - 165 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaximum pressure\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.3 MPa (3 bar)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage buffer\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1x PBS containing 20% ethanol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProduct is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not freeze.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e   \u003cimg alt=\"Keep refrigerated\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Keep-refrigerated_95fa1867-1f0d-47e6-823a-8a0008076e21_small.png?12332456762384261517\" style=\"float: none;\"\u003e   \u003cimg alt=\"Do not freeze\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Do-not-freeze_2_small.png?12332456762384261517\" style=\"float: none;\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003e\u003cbr\u003e\u003c\/h5\u003e\n\u003ch4 style=\"color: #ee8012;\"\u003e\u003cbr\u003e\u003c\/h4\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"25 mL","offer_id":39321581060139,"sku":"11-0222-025","price":302.0,"currency_code":"USD","in_stock":true},{"title":"50 mL","offer_id":28520239697,"sku":"11-0222-050","price":479.0,"currency_code":"USD","in_stock":true},{"title":"100 mL","offer_id":39321581879339,"sku":"11-0222-100","price":830.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/11-0222Ni-IDA-1.png?v=1694786697"},{"product_id":"proteindex-rprotein-a-agarose-gravity-flow-column","title":"PROTEINDEX™ Recombinant Protein A Prepacked Gravity Flow Columns, 1 mL, Pack of 5 Columns","description":"\u003cp\u003e\u003cstrong\u003ePROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e\u003c\/strong\u003e™ recombinant Protein A Agarose Gravity Flow Column is ready-to-use affinity chromatography column for purification of immunoglobulins, notably IgG isotypes, as well as Fc region-containing fusion proteins.\u003c\/p\u003e\n\u003cp\u003eProtein A, a bacterial cell wall protein isolated from \u003cem\u003eStaphylococcus aureus\u003c\/em\u003e, binds to the Fc region of most immunoglobulins through interactions with the heavy chain. Native Protein A contains five high affinity IgG binding domains and many other domains with unknown functions.\u003c\/p\u003e\n\u003cp\u003eThe recombinant Protein A is a genetically engineered protein that contains only the five high affinity IgG binding domains. The non-essential domains were removed to minimize nonspecific binding. The recombinant Protein A is produced in \u003ci\u003eE. coli, \u003c\/i\u003eand it essentially binds to immunoglobulins with the same degree of affinity as the native Protein A. rProtein A is capable of binding with high affinity to the Fc region from IgG of several species including human and rabbit.\u003c\/p\u003e\n\u003cp\u003eEnabled by proprietary ProteIndex™ technology, immobilization of recombinant Protein A on 4% agarose resins was very stable and was optimized for high yield in laboratory preparations and scale-up applications.\u003c\/p\u003e\n\u003cp\u003eEach gravity column contains 1 mL of recombinant Protein A agarose resin, and is individually wrapped.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eProduct Highlights\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eHigh capacity, \u0026gt; 40 mg human IgG per column\u003c\/li\u003e\n\u003cli\u003eHigh selectivity for high purity\u003c\/li\u003e\n\u003cli\u003eMinimal ligand leakage, can be reused\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"5%\"\u003e\u003c\/td\u003e\n\u003ctd width=\"35%\"\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/ProteinA4FF_purifying_mAscites_fluid_3_0d442212-1ce9-4a05-8106-1ec2848dd3cb.png?v=1508439312\"\u003e\u003c\/td\u003e\n\u003ctd valign=\"bottom\"\u003e\n\u003cp\u003e\u003cstrong\u003eHigh purity and high capacity of recombinant Protein A ligand. \u003c\/strong\u003e2 mL mouse ascites fluid was loaded on the column containing 0.5 mL settled rProtein A 4 FF resin. Bound immunoglobulin species were eluted with 100 mM Glycine, pH 3.0. Fractions were analyzed by SDS-PAGE.\u003c\/p\u003e\n\u003cp\u003eLanes: (1) Raw ascites fluid; (2) Flow-through; (3) Elution. Note that, heavy and light chains of IgG were mostly absent from the flow-through, indicating that most immunoglobulins were bound to the column.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"200\"\u003e\u003cstrong\u003eProduct package size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e5 columns\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"200\"\u003e\u003cstrong\u003eColumn volume\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1 mL settled Protein A resins\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eLigand\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eRecombinant protein A produced in \u003cem\u003eE.coli\u003c\/em\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBinding capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;40 mg rabbit IgG\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMatrix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e4% agarose\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eParticle size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e45 μm - 165 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaximum pressure\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.1 MPa (1 bar)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eChemical stability\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eAll commonly used aqueous buffers, including 6 M guanidine-HCl and 8 M urea.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eRecommended flow rate\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eGravity flow, 100 - 300 cm\/hour\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003epH stability\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eLong term: pH 3 - 9; \u003cbr\u003eShort term: pH 2 - 10\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage buffer\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e20% ethanol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eColumn material\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003epolypropylene (body)\u003cbr\u003eLow density polyethylene (top snap cap and bottom tip cap)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eFrit\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003ePolyethylene, with an average pore size of 30 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProduct is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e   \u003cimg style=\"float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Keep-refrigerated_95fa1867-1f0d-47e6-823a-8a0008076e21_small.png?12332456762384261517\" alt=\"Keep refrigerated\"\u003e   \u003cimg style=\"float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Do-not-freeze_2_small.png?12332456762384261517\" alt=\"Do not freeze\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eDocuments\u003c\/h5\u003e\n\u003ch4\u003eTechnical Note\u003c\/h4\u003e\n\u003cp\u003e🗎    \u003ca rel=\"noopener noreferrer\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Table_Binding_characteristics_of_immunoglobulin-binding_proteins.pdf?6231220717744860621\" title=\"Comparison of binding characteristics of immunoglobulin-binding proteins\" target=\"_blank\"\u003eComparison of Binding Characteristics of Immunoglobulin-Binding Proteins\u003c\/a\u003e\u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"5 Columns","offer_id":5915590983719,"sku":"18-0268","price":247.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/ProteinA_gravity_column.png?v=1512006816"},{"product_id":"magindex-rprotein-a-magnetic-agarose-resin","title":"MagINDEX™ rProtein A Magnetic Agarose, Settled Resin","description":"\u003cp\u003e\u003cstrong\u003eMag\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e\u003c\/strong\u003e™ rProtein A Magnetic Agarose is an affinity chromatography medium designed for rapid, easy, one-step purification of immunoglobulins, notably IgG isotypes, as well as Fc region-containing fusion proteins.\u003c\/p\u003e\n\u003cp\u003eProprietary MagIndex\u003cb\u003e™\u003c\/b\u003e technology facilitates encapsulation of the superparamagnetic core in the mechanically stable Agarose matrix, which is the optimal absorbance for high yield protein purification with minimal background.\u003c\/p\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"15%\"\u003e\u003c\/td\u003e\n\u003ctd width=\"70%\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Schematic_composition_of_mag_agarose_resins_862e6d64-9839-4cc9-95d6-b8f9f1b47dcf.png?v=1527170756\" alt=\"\"\u003e\u003c\/td\u003e\n\u003ctd width=\"15%\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003eThe superparamagnetic core has very fast magnetic response in the magnetic field, but no memory after the magnetic force is removed. The agarose-based encapsulation provides a strong shield well protecting the magnetic core in a wide range of reagents that are commonly used in protein purification. Moreover, as an ideal matrix for protein purification, the agarose matrix enables purification of antibodies and Ig fragments with high purity and high capacity, in high throughput screening and various preparative scales.\u003c\/p\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cdiv class=\"video-wrapper\"\u003e\u003ciframe src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/MagIndex_Magnetic_Agarose_Resins-3.mp4?14800512398908436554\"\u003e\u003c\/iframe\u003e\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd width=\"50%\" valign=\"top\"\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cspan style=\"color: #444444;\"\u003e\u003cstrong\u003eSuperparamagnetic core\u003c\/strong\u003e with fast response to magnetic field\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan style=\"color: #444444;\"\u003e\u003cstrong\u003eLow nonspecific binding\u003c\/strong\u003e for assay accuracy\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan style=\"color: #444444;\"\u003e\u003cstrong\u003eHigh density of the Protein A\u003c\/strong\u003e ligand for optimal yield of antibodies and Ig fragments\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan style=\"color: #444444;\"\u003e\u003cstrong\u003eHigh stability\u003c\/strong\u003e in common buffer systems and detergents\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProtein A, a bacterial cell wall protein isolated from \u003cem\u003eStaphylococcus aureus\u003c\/em\u003e, binds to the Fc region of most immunoglobulins through interactions with the heavy chain. Native Protein A contains five high affinity IgG binding domains and many other domains with unknown functions.\u003c\/p\u003e\n\u003cp\u003eThe recombinant Protein A is a genetically engineered protein that contains only the five high affinity IgG binding domains. The non-essential domains were removed to minimize nonspecific binding. The recombinant Protein A is produced in \u003ci\u003eE. coli, \u003c\/i\u003eand it essentially binds to immunoglobulins with the same degree of affinity as the native Protein A. rProtein A is capable of binding with high affinity to the Fc region from IgG of several species including human and rabbit.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eProduct Highlights\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eHigh capacity, \u0026gt; 10 mg rabbit IgG \/ mL of settled resins\u003c\/li\u003e\n\u003cli\u003eHigh selectivity for high purity\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eApplications\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eQuick isolation of antibodies from sera and ascites fluids\u003c\/li\u003e\n\u003cli\u003ePurification of scFc fragment from cell cultures\u003c\/li\u003e\n\u003cli\u003eDepletion of unwanted antibodies and fragments from sample\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eTech Note\u003c\/span\u003e\u003c\/h4\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"25%\" valign=\"top\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/MagIndex_Protein_A-4.png?14389587024720716232\" alt=\"\"\u003e\u003c\/td\u003e\n\u003ctd valign=\"bottom\"\u003e\n\u003cp\u003e\u003cstrong\u003eRecombinant scFc protein was isolated from cell culture with MagIndex rProtein A Magnetic Agarose resins. \u003c\/strong\u003eBound the scFc protein was eluted with 100 mM Glycine, pH 3.0, denoted by the blue arrow. Fractions were analyzed by SDS-PAGE.\u003c\/p\u003e\n\u003cp\u003eLanes: (L) Culture supernatant; (FT) Flow-through; (E) Elutions 1 and 2.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSample:\u003c\/strong\u003e 10 mL of culture supernatant containing a scFc fragment (20 µg\/mL)\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eResin:\u003c\/strong\u003e 0.2 mL settle resins of MagIndex Protein A magnetic agarose\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBinding:\u003c\/strong\u003e 45 min at room temperature\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eWashing:\u003c\/strong\u003e 4 mL binding buffer\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eElution:\u003c\/strong\u003e Incubate in 100 mM Glycine, pH 3.0 for 10 min. Repeat 3 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"200\"\u003e\u003cstrong\u003eLigand\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eRecombinant protein A containing only Ig binding domains\u003cem\u003e\u003cbr\u003e\u003c\/em\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBinding capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;10 mg rabbit IgG\/mL medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eMatrix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eCross-linked agarose beaded particles containing the superparamagnetic core, supplied as a 20% slurry\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eParticle size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e30 μm - 100 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003epH stability\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003eLong term: pH 2 - 9;\u003c\/p\u003e\n\u003cp\u003eShort term: pH 2 - 11\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage buffer\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003ePBS containing 0.01% Tween-20 and 0.02% NaN\u003csub\u003e3\u003c\/sub\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProduct is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e   \u003cimg alt=\"Keep refrigerated\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Keep-refrigerated_95fa1867-1f0d-47e6-823a-8a0008076e21_small.png?12332456762384261517\" style=\"float: none;\"\u003e   \u003cimg alt=\"Do not freeze\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Do-not-freeze_2_small.png?12332456762384261517\" style=\"float: none;\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eDocuments\u003c\/h5\u003e\n\u003ch4\u003eTechnical Note\u003c\/h4\u003e\n\u003cp\u003e🗎    \u003ca title=\"Comparison of binding characteristics of immunoglobulin-binding proteins\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Table_Binding_characteristics_of_immunoglobulin-binding_proteins.pdf?6231220717744860621\" rel=\"noopener noreferrer\" target=\"_blank\"\u003eComparison of Binding Characteristics of Immunoglobulin-Binding Proteins\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"2 mL","offer_id":6599808221223,"sku":"11-0271-002","price":225.0,"currency_code":"USD","in_stock":true},{"title":"5 mL","offer_id":6600507916327,"sku":"11-0271-005","price":491.0,"currency_code":"USD","in_stock":true},{"title":"10 mL","offer_id":6600507949095,"sku":"11-0271-010","price":710.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/MagIndex_Protein_A.png?v=1525138379"},{"product_id":"empty-disposable-gravity-flow-columns-300-ml-with-50-um-frits","title":"Empty Disposable Gravity Flow Columns, 300 mL with 50 µm frits","description":"\u003cp\u003eThese disposable columns are economical, versatile columns for single-use purification applications using gravity flow.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eSimple, cost-effective, readily adaptable for a wide range of gravity flow applications\u003c\/li\u003e\n\u003cli\u003eMade of high purity medical grade polypropylene, stable in commonly used chemicals and reagents\u003c\/li\u003e\n\u003cli\u003eAvailable in various sizes to suit for different experiments\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eEach empty column comes with one end cap, one retaining ring, and a filter disc inserted as bed support. An extra filter disc is also provided for extra support and cleaning flow through when needed. The filter disc can also be placed on top of the gravity-settled medium bed to prevent the medium from drying during preps and storage. The ring is optional. When it is used, it helps keeping the top frit in place during the chromatography application. \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eProduct Details\u003c\/span\u003e\u003c\/h4\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"200\"\u003e\u003cstrong\u003eMaximum capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e343 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBed volume\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eup to 250 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eColumn inner diameter\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e49.4 mm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eColumn height\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e180 mm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaterial (column)\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003ePolypropylene (PP)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaterial (filter disc)\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUltrahigh molecular weight polyethylene (UHMW-PE), hydrophilic *\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaterial (stopcock)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003ePolycarbonates (PC) body, HDPE diverter\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eFilter pore size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e50 µm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1 empty column\u003c\/p\u003e\n\u003cp\u003e1 top plug\u003c\/p\u003e\n\u003cp\u003e1 end cap\u003c\/p\u003e\n\u003cp\u003e2 filter discs (one is inserted and the other is provided as optional extra)\u003c\/p\u003e\n\u003cp\u003e1-way stopcock, female luer to male luer (for Bundled with Stopcocks only)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eChemical stability\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStable in all commonly used reagents\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFor fast, general-purpose purification by gravity flow or low pressure:\n\u003cul style=\"padding-left: 60px;\"\u003e\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003eAffinity chromatography of protein or other macromolecules\u003c\/li\u003e\n\u003cli\u003eImmunodepletion of immune reactive complexes\u003c\/li\u003e\n\u003cli\u003eSize-exclusion chromatography\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul style=\"padding-left: 60px;\"\u003e\u003c\/ul\u003e\n\u003cstrong\u003e\u003cem\u003eFor research use only. Not intended for any human or animal therapeutic or diagnostic uses.\u003c\/em\u003e\u003c\/strong\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e* Hydrophobic version of filter discs is available upon request. \u003ca href=\"mailto:cust.support@marvelgent.com?subject=Request%20for%20300%20mL%20column%20with%2050%20um%20hydrophobic%20frits\"\u003eContact us\u003c\/a\u003e for more detail. \u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eWe provide a variety of columns to fit your application needs. \u003ca title=\"Empty Disposable Gravity Flow Columns\" href=\"https:\/\/marvelgent.com\/collections\/empty-disposable-gravity-flow-columns#top\"\u003eView our collection and selection disposable gravity columns for your application \u0026gt;\u0026gt;\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003e\u003c\/h5\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"Pack of 5","offer_id":11256398676011,"sku":"12-0280-005","price":158.0,"currency_code":"USD","in_stock":true},{"title":"Pack of 10","offer_id":11256398708779,"sku":"12-0280-010","price":272.0,"currency_code":"USD","in_stock":true},{"title":"Pack of 5, Bundled with Stopcocks","offer_id":17194121756715,"sku":"12-0280-005SC","price":184.0,"currency_code":"USD","in_stock":true},{"title":"Pack of 10, Bundled with Stopcocks","offer_id":17194124935211,"sku":"12-0280-010SC","price":314.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/300_ml_disposable_column_4.png?v=1610994038"},{"product_id":"empty-disposable-gravity-flow-columns-30-ml-with-50-um-frits","title":"Empty Disposable Gravity Flow Columns, Fritted, 30 mL, with 50 µm frits","description":"\u003cp\u003eThese disposable columns are economical, versatile columns for single-use purification applications using gravity flow.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eSimple, cost-effective, readily adaptable for a wide range of gravity flow applications\u003c\/li\u003e\n\u003cli\u003eMade of high purity medical grade polypropylene, stable in commonly used chemicals and reagents\u003c\/li\u003e\n\u003cli\u003eAvailable in various sizes to suit for different experiments\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eEach empty column comes with one each of the top and end caps, as well as a filter disc as bed support. An extra filter disc is also provided and can be used as upper bed support. Placing a filter disc on top of the gravity-settled medium bed helps prevent drying of the medium.  \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eProduct Details\u003c\/span\u003e\u003c\/h4\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"200\"\u003e\u003cstrong\u003eMaximum capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e45.8 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBed volume\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0 - 25 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eColumn inner diameter\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e22.5 mm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eColumn height\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e105.7 mm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaterial (column)\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003ePolypropylene (PP)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaterial (filter disc)\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003ePolyethylene (PE)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eFilter pore size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e50 µm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1 empty column, with luer-lock outlet\u003c\/p\u003e\n\u003cp\u003e1 top cap\u003c\/p\u003e\n\u003cp\u003e1 end cap\u003c\/p\u003e\n\u003cp\u003e2 filter discs (one is inserted and the other is provided as optional extra)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eChemical stability\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStable in all commonly used reagents\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFor fast, general-purpose purification by gravity flow or low pressure:\n\u003cul style=\"padding-left: 60px;\"\u003e\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003eAffinity chromatography of protein or other macromolecules\u003c\/li\u003e\n\u003cli\u003eImmunodepletion of immune reactive complexes\u003c\/li\u003e\n\u003cli\u003eSize-exclusion chromatography\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul style=\"padding-left: 60px;\"\u003e\u003c\/ul\u003e\n\u003cstrong\u003e\u003cem\u003eFor research use only. Not intended for any human or animal therapeutic or diagnostic uses.\u003c\/em\u003e\u003c\/strong\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eWe provide a variety of columns to fit your application needs. \u003ca href=\"https:\/\/marvelgent.com\/collections\/empty-disposable-gravity-flow-columns#top\" title=\"Empty Disposable Gravity Flow Columns\"\u003eView our collection and selection disposable gravity columns for your application \u0026gt;\u0026gt;\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"Pack of 25","offer_id":11257602801707,"sku":"12-0278-025","price":183.0,"currency_code":"USD","in_stock":true},{"title":"Pack of 50","offer_id":11257602834475,"sku":"12-0278-050","price":341.0,"currency_code":"USD","in_stock":true},{"title":"Pack of 25, Bundled with Stopcocks","offer_id":40808351301675,"sku":"12-0278-025SC","price":257.0,"currency_code":"USD","in_stock":true},{"title":"Pack of 50, Bundled with Stopcocks","offer_id":40808351989803,"sku":"12-0278-050SC","price":491.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/30_ml_disposable_columns_ad408fcc-fae8-4be1-9a36-b099d3c6d851.png?v=1543442996"},{"product_id":"empty-disposable-gravity-flow-columns-60-ml-with-50-um-frits","title":"Empty Disposable Gravity Flow Columns, 60 mL with 50 µm frits","description":"\u003cp\u003eThese disposable columns are economical, versatile columns for single-use purification applications using gravity flow.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eSimple, cost-effective, readily adaptable for a wide range of gravity flow applications\u003c\/li\u003e\n\u003cli\u003eMade of high purity medical grade polypropylene, stable in commonly used chemicals and reagents\u003c\/li\u003e\n\u003cli\u003eAvailable in various sizes to suit for different experiments\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eEach empty column comes with one each of the top and end caps, as well as a filter disc as bed support. An extra filter disc is also provided and can be used as upper bed support. Placing a filter disc on top of the gravity-settled medium bed helps prevent drying of the medium.  \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eProduct Details\u003c\/span\u003e\u003c\/h4\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"200\"\u003e\u003cstrong\u003eMaximum capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e74 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBed volume\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0 - 50 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eColumn inner diameter\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e26.4 mm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eColumn height\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e134.8 mm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaterial (column)\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003ePolypropylene (PP)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaterial (filter disc)\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003ePolyethylene (PE)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaterial (stopcock)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003ePolycarbonates (PC) body, HDPE diverter\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eFilter pore size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e50 µm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1 empty column\u003c\/p\u003e\n\u003cp\u003e1 top plug\u003c\/p\u003e\n\u003cp\u003e1 end cap\u003c\/p\u003e\n\u003cp\u003e2 filter discs (one is inserted and the other is provided as optional extra)\u003c\/p\u003e\n\u003cp\u003e1-way stopcock, female luer to male luer (for Bundled with Stopcocks only)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eChemical stability\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStable in all commonly used reagents\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFor fast, general-purpose purification by gravity flow or low pressure:\n\u003cul style=\"padding-left: 60px;\"\u003e\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003eAffinity chromatography of protein or other macromolecules\u003c\/li\u003e\n\u003cli\u003eImmunodepletion of immune reactive complexes\u003c\/li\u003e\n\u003cli\u003eSize-exclusion chromatography\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul style=\"padding-left: 60px;\"\u003e\u003c\/ul\u003e\n\u003cstrong\u003e\u003cem\u003eFor research use only. Not intended for any human or animal therapeutic or diagnostic uses.\u003c\/em\u003e\u003c\/strong\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cbr\u003e\n\u003cp\u003eWe provide a variety of columns to fit your application needs. \u003ca href=\"https:\/\/marvelgent.com\/collections\/empty-disposable-gravity-flow-columns#top\" title=\"Empty Disposable Gravity Flow Columns\"\u003eView our collection and selection disposable gravity columns for your application \u0026gt;\u0026gt;\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"Pack of 20","offer_id":11258905067563,"sku":"12-0279-020","price":246.0,"currency_code":"USD","in_stock":true},{"title":"Pack of 40","offer_id":11258905100331,"sku":"12-0279-040","price":491.0,"currency_code":"USD","in_stock":true},{"title":"Pack of 20, Bundled with Stopcocks","offer_id":40087808901163,"sku":"12-0279-020SC","price":337.0,"currency_code":"USD","in_stock":true},{"title":"Pack of 40, Bundled with Stopcocks","offer_id":40087808966699,"sku":"12-0279-040SC","price":646.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/60_ml_disposable_columns_2.png?v=1565639469"},{"product_id":"proteindex-dextrin-agarose-6-fast-flow","title":"PROTEINDEX™ Dextrin Agarose 6 Fast Flow","description":"\u003cp\u003e\u003cstrong\u003ePROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e\u003c\/strong\u003e™ \u003cstrong\u003eDextrin Agarose 6FF\u003c\/strong\u003e is a chromatography medium for purifying proteins fused to maltose binding protein (MBP-tagged protein). Recombinant proteins with the MBP-tag often have increased expression levels and higher solubility. Proper folding of the attached protein has also been shown to be promoted by the MBP tag. Since MBP increase the solubility, the tag is extremely useful for recombinant proteins that are prone to be accumulated in an insoluble form (inclusion bodies).\u003c\/p\u003e\n\u003cp\u003e\u003cspan class=\"st\" data-hveid=\"99\" data-ved=\"0ahUKEwifgtvOqo3bAhVozlkKHfsAAt8Q4EUIYzAG\"\u003eDextrin is the polymeric product resulted by enzymatic hydrolysis of amylose with beta amylase. Compared to Amylose that is susceptible to amylase degradation, Dextrin is very stable. When used as the ligand substrate immobilized on the resin for purification of the MBP tag, Dextrin offers consistently strong affinity absorption even after repeated uses. \u003cbr\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan class=\"st\" data-hveid=\"99\" data-ved=\"0ahUKEwifgtvOqo3bAhVozlkKHfsAAt8Q4EUIYzAG\"\u003eAffinity purification using Dextrin Agarose 6 Fast Flow take place under physiological conditions and mild elution is performed using maltose which preserve target protein activity. These mild conditions may even allow purification of intact protein complexes.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eThe base matrix of Dextrin Agarose 6 Fast Flow is a robust, highly cross- linked 6% agarose. The crosslinking of the base matrix has been optimized to give high physical and chemical stability, high specificity of the binding.\u003c\/p\u003e\n\u003cp\u003eOur proprietary ProteIndex™ coupling technology ensures stable immobilization of active ligand and minimal ligand leakage in a wide range of purification buffer conditions. The resins can be reused multiple times to purify the same target protein.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eProduct Highlights\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eHigh selectivity for high purity\u003c\/li\u003e\n\u003cli\u003e\u0026gt; 10 mg MBP tagged protein (80 kDa)\/mL medium\u003c\/li\u003e\n\u003cli\u003eStable coupling of ligand, low leakage; can be re-used to purify the same protein\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003e Technical Notes\u003c\/span\u003e\u003c\/h4\u003e\n\u003cp\u003e\u003cstrong\u003e \u003c\/strong\u003e \u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Dextran_6FF_3.png?v=1561146279\" alt=\"\" style=\"display: block; margin-left: auto; margin-right: auto;\" width=\"324\" height=\"250\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFigure 1. Purification performance of Dextrin 6FF resins.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e50 mL of \u003ci\u003eE.coli \u003c\/i\u003elysate containing a MBP-tagged fusion protein (~ 80 kDa) was loaded on 0.5 mL packed Dextrin 6FF resins at 0.5 mL\/mL. The column was then washed with 20 mL of Binding Buffer (20 mM Tris-HCl, 200 mM NaCl, 1 mM EDTA, pH7.4). The target MBP-tagged protein was eluted with 9.5 mL of elution buffer (20 mM Tris-HCl, 200 mM NaCl, 1 mM EDTA, 10 mM maltose, pH7.4). Fractions of lysate, flow-through, and eluate were analysed by SDS-PAGE. Lanes: (1) Raw cell lysate;  (2) Flow-through; (3) Eluate.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"200\"\u003e\u003cstrong\u003eLigand\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eDextrin\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBinding capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;10 mg MBP tagged protein (80 kDa)\/mL medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMatrix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e6% highly cross-linked agarose supplied as 50% slurry\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eParticle size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e45 μm - 165 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaximum pressure\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.3 MPa (3 bar, 43 psi)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eRecommended flow rate\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003e300 - 500 cm\/hour\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003epH stability\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003e3-12\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage buffer\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e20% ethanol as preservative\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProduct is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e   \u003cimg alt=\"Keep refrigerated\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Keep-refrigerated_95fa1867-1f0d-47e6-823a-8a0008076e21_small.png?12332456762384261517\" style=\"float: none;\"\u003e   \u003cimg alt=\"Do not freeze\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Do-not-freeze_2_small.png?12332456762384261517\" style=\"float: none;\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"10 mL","offer_id":12143023816747,"sku":"11-0218-010","price":147.0,"currency_code":"USD","in_stock":true},{"title":"25 mL","offer_id":12143023882283,"sku":"11-0218-025","price":317.0,"currency_code":"USD","in_stock":true},{"title":"100 mL","offer_id":12143023915051,"sku":"11-0218-100","price":1062.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/Dextran_6FF.png?v=1559231595"},{"product_id":"magindex-rprotein-a-g-magnetic-agarose-settled-resin","title":"MagINDEX™ rProtein A\/G Magnetic Agarose, Settled Resin","description":"\u003cp\u003e\u003cstrong\u003eMag\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e\u003c\/strong\u003e™ rProtein A\/G Magnetic Agarose is an affinity chromatography medium designed for rapid, easy, one-step purification of a wide range of immunoglobulins, as well as Fc region-containing fusion proteins.\u003c\/p\u003e\n\u003cp\u003eProprietary MagIndex\u003cb\u003e™\u003c\/b\u003e technology facilitates encapsulation of the superparamagnetic core in the mechanically stable Agarose matrix, which is the optimal absorbance for high yield protein purification with minimal background.\u003c\/p\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"15%\"\u003e\u003c\/td\u003e\n\u003ctd width=\"70%\"\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Schematic_composition_of_mag_agarose_resins_862e6d64-9839-4cc9-95d6-b8f9f1b47dcf.png?v=1527170756\"\u003e\u003c\/td\u003e\n\u003ctd width=\"15%\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003eThe superparamagnetic core has very fast magnetic response in the magnetic field, but no memory after the magnetic force is removed. The agarose-based encapsulation provides a strong shield well protecting the magnetic core in a wide range of reagents that are commonly used in protein purification. Moreover, as an ideal matrix for protein purification, the agarose matrix enables purification of antibodies and Ig fragments with high purity and high capacity, in high throughput screening and various preparative scales.\u003c\/p\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cdiv class=\"video-wrapper\"\u003e\u003ciframe src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/MagIndex_Magnetic_Agarose_Resins-3.mp4?14800512398908436554\"\u003e\u003c\/iframe\u003e\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"50%\"\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cspan style=\"color: #444444;\"\u003e\u003cstrong\u003eSuperparamagnetic core\u003c\/strong\u003e with fast response to magnetic field\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan style=\"color: #444444;\"\u003e\u003cstrong\u003eLow nonspecific binding\u003c\/strong\u003e for assay accuracy\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan style=\"color: #444444;\"\u003e\u003cstrong\u003eHigh density of the Protein A\/G\u003c\/strong\u003e ligand for optimal yield of antibodies and Ig fragments\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan style=\"color: #444444;\"\u003e\u003cstrong\u003eHigh stability\u003c\/strong\u003e in common buffer systems and detergents\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProtein A, a bacterial cell wall protein isolated from \u003cem\u003eStaphylococcus aureus\u003c\/em\u003e, binds to the Fc region of most immunoglobulins through interactions with the heavy chain. Native Protein A contains five high affinity IgG binding domains and many other domains with unknown functions. While the native protein G has 3 IgG binding domains as well as sites for albumin and cell-surface binding, recombinant protein G has only Ig recognition domains but no other non-specific sequences. \u003c\/p\u003e\n\u003cp\u003eRecombinant Protein A\/G is a recombinant chimeric protein that is composed of the IgG binding domains of both Protein A and Protein G. rProtein A\/G contains five binding domains from Protein A and two from Protein G. The binding of rProtein A\/G is less pH-dependent than Protein A, but otherwise has the additive properties of Protein A and G. Therefore, rProtein A\/G is ideal for binding the broadest range of IgG subclasses from rabbit, mouse, human and other mammalian samples.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eProduct Highlights\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eHigh capacity, \u0026gt; 10 mg rabbit IgG \/ mL of settled resins\u003c\/li\u003e\n\u003cli\u003eHigh selectivity for high purity\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eApplications\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eQuick isolation of antibodies from sera and ascites fluids\u003c\/li\u003e\n\u003cli\u003ePurification of recombinant antibody or scFc fragment from cell cultures\u003c\/li\u003e\n\u003cli\u003eDepletion of unwanted antibodies and fragments from sample\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"200\"\u003e\u003cstrong\u003eLigand\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eRecombinant chimeric protein A\/G containing only Ig binding domains\u003cem\u003e\u003cbr\u003e\u003c\/em\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBinding capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;10 mg rabbit IgG\/mL medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eMatrix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eCross-linked agarose beaded particles containing the superparamagnetic core, supplied as a 20% slurry\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eParticle size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e30 μm - 100 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003epH stability\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003eLong term: pH 2 - 9;\u003c\/p\u003e\n\u003cp\u003eShort term: pH 2 - 11\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage buffer\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003ePBS containing 0.01% Tween-20 and 0.02% NaN\u003csub\u003e3\u003c\/sub\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProduct is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e   \u003cimg style=\"float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Keep-refrigerated_95fa1867-1f0d-47e6-823a-8a0008076e21_small.png?12332456762384261517\" alt=\"Keep refrigerated\"\u003e   \u003cimg style=\"float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Do-not-freeze_2_small.png?12332456762384261517\" alt=\"Do not freeze\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eDocuments\u003c\/h5\u003e\n\u003ch4\u003eTechnical Note\u003c\/h4\u003e\n\u003cp\u003e🗎    \u003ca rel=\"noopener noreferrer\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Table_Binding_characteristics_of_immunoglobulin-binding_proteins.pdf?6231220717744860621\" title=\"Comparison of binding characteristics of immunoglobulin-binding proteins\" target=\"_blank\"\u003eComparison of Binding Characteristics of Immunoglobulin-Binding Proteins\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"2 mL","offer_id":12406185754667,"sku":"11-0273-002","price":376.0,"currency_code":"USD","in_stock":true},{"title":"5 mL","offer_id":12406185787435,"sku":"11-0273-005","price":826.0,"currency_code":"USD","in_stock":true},{"title":"10 mL","offer_id":12406185820203,"sku":"11-0273-010","price":1207.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/MagIndex_Protein_A_d9a5f606-cc94-42ef-9a01-9a1543202424.png?v=1528207075"},{"product_id":"empty-disposable-gravity-flow-columns-60-ml-with-20-um-frits","title":"Empty Disposable Gravity Flow Columns, 60 mL with 20 µm frits","description":"\u003cp\u003eThese disposable columns are economical, versatile columns for single-use purification applications using gravity flow.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eSimple, cost-effective, readily adaptable for a wide range of gravity flow applications\u003c\/li\u003e\n\u003cli\u003eMade of high purity medical grade polypropylene, stable in commonly used chemicals and reagents\u003c\/li\u003e\n\u003cli\u003eAvailable in various sizes to suit for different experiments\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eEach empty column comes with one each of the top and end caps, as well as a filter disc as bed support. An extra filter disc is also provided and can be used as upper bed support. Placing a filter disc on top of the gravity-settled medium bed helps prevent drying of the medium.  \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eProduct Details\u003c\/span\u003e\u003c\/h4\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"200\"\u003e\u003cstrong\u003eMaximum capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e74 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBed volume\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0 - 50 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eColumn inner diameter\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e26.4 mm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eColumn height\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e134.8 mm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaterial (column)\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003ePolypropylene (PP)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaterial (filter disc)\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003ePolyethylene (PE)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaterial (stopcock)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003ePolycarbonates (PC) body, HDPE diverter\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eFilter pore size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e20 µm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1 empty column\u003c\/p\u003e\n\u003cp\u003e1 top plug\u003c\/p\u003e\n\u003cp\u003e1 end cap\u003c\/p\u003e\n\u003cp\u003e2 filter discs (one is inserted and the other is provided as optional extra)\u003c\/p\u003e\n\u003cp\u003e1-way stopcock, female luer to male luer (for Bundled with Stopcocks only)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eChemical stability\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStable in all commonly used reagents\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFor fast, general-purpose purification by gravity flow or low pressure:\n\u003cul style=\"padding-left: 60px;\"\u003e\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003eAffinity chromatography of protein or other macromolecules\u003c\/li\u003e\n\u003cli\u003eImmunodepletion of immune reactive complexes\u003c\/li\u003e\n\u003cli\u003eSize-exclusion chromatography\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul style=\"padding-left: 60px;\"\u003e\u003c\/ul\u003e\n\u003cstrong\u003e\u003cem\u003eFor research use only. Not intended for any human or animal therapeutic or diagnostic uses.\u003c\/em\u003e\u003c\/strong\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cbr\u003e\n\u003cp\u003eWe provide a variety of columns to fit your application needs. \u003ca href=\"https:\/\/marvelgent.com\/collections\/empty-disposable-gravity-flow-columns#top\" title=\"Empty Disposable Gravity Flow Columns\"\u003eView our collection and selection disposable gravity columns for your application \u0026gt;\u0026gt;\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"Pack of 20","offer_id":16952792776747,"sku":"11-0282-020","price":246.0,"currency_code":"USD","in_stock":true},{"title":"Pack of 40","offer_id":16952792809515,"sku":"11-0282-040","price":491.0,"currency_code":"USD","in_stock":true},{"title":"Pack of 20, Bundled with Stopcocks","offer_id":40087794024491,"sku":"11-0282-020SC","price":334.0,"currency_code":"USD","in_stock":true},{"title":"Pack of 40, Bundled with Stopcocks","offer_id":40087796187179,"sku":"11-0282-040SC","price":646.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/60_ml_disposable_columns_2_58cca76a-3970-489e-9529-1c0506a710b1.png?v=1565639484"},{"product_id":"one-way-stopcock-valve-polypropylene-female-luer-to-male-luer","title":"One-Way Stopcock Valve, Polypropylene, Female Luer to Male Luer","description":"\u003cp\u003eThese 1-way stopcocks are constructed of polypropylene for strength, thermal performance, and solvent resistance. They have been widely used in SPE solid phase extraction and chromatography applications that use attacking agents, such as Acetone, Acetic Acid, \u003cspan data-mce-fragment=\"1\" class=\"aCOpRe\"\u003eIsopropyl Alcohol\u003c\/span\u003e, Ethanol, and Butyl Alcohols. \u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eThe standard female luer-lock inlet and male luer slip outlet are compatible with most commonly used vacuum manifolds. \u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eProduct Details\u003c\/span\u003e\u003c\/h4\u003e\n\u003ctable data-mce-selected=\"1\" width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd data-mce-selected=\"1\" width=\"200\"\u003e\u003cstrong\u003eMaterial\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd data-mce-selected=\"1\"\u003ePolypropylene\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd data-mce-selected=\"1\"\u003e\u003cstrong\u003eOperating Pressure\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd data-mce-selected=\"1\"\u003eGravity, and low to medium vacuum\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003e \u003c\/span\u003e\u003c\/h4\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eProduct Highlights\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eAllows optimization of sample flow rates during phase separation and extraction;\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003eTolerates a broader range of solvents and chemicals than the polycarbonate- or nylon-made devices;\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003eBarbed for more secure connection to tubing;\u003c\/li\u003e\n\u003cli\u003eOperates under low to medium vacuum pressure;\u003c\/li\u003e\n\u003cli\u003eCompatible with steam sterilization (autoclaving).\u003cbr\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cem\u003eFor research use only. Not intended for any human or animal therapeutic or diagnostic uses.\u003c\/em\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eWe provide a variety of columns to fit your application needs. \u003ca title=\"Empty Disposable Gravity Flow Columns\" href=\"https:\/\/marvelgent.com\/collections\/empty-disposable-gravity-flow-columns#top\"\u003eView our collection and selection disposable gravity columns for your application \u0026gt;\u0026gt;\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003e\u003c\/h5\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"Pack of 12","offer_id":31921880530987,"sku":"18-0288-012","price":133.0,"currency_code":"USD","in_stock":true},{"title":"Pack of 20","offer_id":31857846386731,"sku":"18-0288-020","price":159.0,"currency_code":"USD","in_stock":true},{"title":"Pack of 40","offer_id":31857846419499,"sku":"18-0288-040","price":287.0,"currency_code":"USD","in_stock":true},{"title":"Pack of 100","offer_id":31857848811563,"sku":"18-0288-100","price":667.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/PPstopcock_SUM1.png?v=1623895650"},{"product_id":"one-way-stopcock-valve-pc-body-hdpe-plug-female-luer-to-male-luer","title":"One-Way Stopcock Valve, PC Body, HDPE Plug, Female Luer to Male Luer","description":"\u003cp\u003eA stopcock provides the on-off flow control of a liquid during chromatography.\u003c\/p\u003e\n\u003cp\u003eThese stopcocks are 1-way and have female Luer-lock inlet and male Luer slip outlet. \u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eProduct Details\u003c\/span\u003e\u003c\/h4\u003e\n\u003cp\u003ePolycarbonate (PC) body, and natural HDPE diverter.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cem\u003eFor research use only. Not intended for any human or animal therapeutic or diagnostic uses.\u003c\/em\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eWe provide a variety of columns to fit your application needs. \u003ca href=\"https:\/\/marvelgent.com\/collections\/empty-disposable-gravity-flow-columns#top\" title=\"Empty Disposable Gravity Flow Columns\"\u003eView our collection and selection disposable gravity columns for your application \u0026gt;\u0026gt;\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003e\u003c\/h5\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"Pack of 10","offer_id":31880303247403,"sku":"16-0289-010","price":83.0,"currency_code":"USD","in_stock":true},{"title":"Pack of 100","offer_id":31880303280171,"sku":"16-0289-100","price":630.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/PCstopcock.png?v=1608741859"},{"product_id":"empty-disposable-gravity-flow-columns-starter","title":"Empty Disposable Gravity Flow Columns, Starter Pack","description":"\u003cp\u003eThese disposable columns are economical, versatile columns for single-use purification applications using gravity flow.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eSimple, cost-effective, readily adaptable for a wide range of gravity flow applications\u003c\/li\u003e\n\u003cli\u003eMade of high purity medical grade polypropylene, stable in commonly used chemicals and reagents\u003c\/li\u003e\n\u003cli\u003eAvailable in various sizes to suit for different experiments\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eEach empty column comes with one each of the top and end caps, as well as a filter disc as bed support. An extra filter disc is also provided and can be used as upper bed support. Placing a filter disc on top of the gravity-settled medium bed helps prevent drying of the medium.  \u003c\/p\u003e\n\u003cp\u003eThe starter pack contains: 10 of 3 mL sets, 10 of 6 mL sets, 5 of 12 mL sets, 3 of 30 mL sets, 3 of 60 mL sets, and 1 of 300 mL set.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eProduct Details\u003c\/span\u003e\u003c\/h4\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"200\"\u003e\u003cstrong\u003eMaximum capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eVarious\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaterial (column)\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003ePolypropylene (PP)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaterial (filter disc)\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003ePolyethylene (PE)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaterial (stopcock)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003ePolycarbonates (PC) body, HDPE diverter\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eFilter pore size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e20 µm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e1 empty column\u003c\/p\u003e\n\u003cp\u003e1 top plug\u003c\/p\u003e\n\u003cp\u003e1 end cap\u003c\/p\u003e\n\u003cp\u003e2 filter discs (one is inserted and the other is provided as optional extra)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eChemical stability\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStable in all commonly used reagents\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFor fast, general-purpose purification by gravity flow or low pressure:\n\u003cul style=\"padding-left: 60px;\"\u003e\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003eAffinity chromatography of protein or other macromolecules\u003c\/li\u003e\n\u003cli\u003eImmunodepletion of immune reactive complexes\u003c\/li\u003e\n\u003cli\u003eSize-exclusion chromatography\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul style=\"padding-left: 60px;\"\u003e\u003c\/ul\u003e\n\u003cstrong\u003e\u003cem\u003eFor research use only. Not intended for any human or animal therapeutic or diagnostic uses.\u003c\/em\u003e\u003c\/strong\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cbr\u003e\n\u003cp\u003eWe provide a variety of columns to fit your application needs. \u003ca href=\"https:\/\/marvelgent.com\/collections\/empty-disposable-gravity-flow-columns#top\" title=\"Empty Disposable Gravity Flow Columns\"\u003eView our collection and selection disposable gravity columns for your application \u0026gt;\u0026gt;\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"Various sizes","offer_id":32868740300843,"sku":"18-0290","price":152.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/Emptycolumnfamily.png?v=1600291157"},{"product_id":"mwco-ultrafiltration-spin-units","title":"MWCO Ultrafiltration Spin Units, 12 mL, Pack of 24","description":"\u003cp\u003eUltrafiltration spin units offer a simple, one step procedure for desalting and concentration of proteins and other macromolecules with greater than 90% recovery. Utilizing molecular weight cut-off polyethersulfone (PES) filter membrane, the spin device is able to retain macromolecules having the greater MW, and filtrate salts and other molecules of the smaller MW. Because of low protein\/DNA binding properties of the PES filter, loss of the retentate on the filter is minimized. The retentate can be recovered at excellent rate. \u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eFour molecular weight cut-off (MWCO) sizes are provided to cover a broad range of molecular weights: 10 K, 30 K, 50 K, and 100 K. It is recommended to choose an MWCO 1\/2 to 1\/3 smaller than the protein to be concentrated. For instance, to concentrate a protein of 80 kDa, we recommend to start with the 30 K MWCO spin unit. \u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eProduct Highlights\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eSimple, rapid spin for simultaneous desalting and concentrating (up to 75-fold concentration); \u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003eBuffer exchange in two quick steps;\u003c\/li\u003e\n\u003cli\u003eFaster filtration - high flux PES membrane is heat-sealed to the housing unit;\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003eHigh recovery of the retentate - the PES membrane has very low absorption property for protein and DNA\/RNA and the filter housing unit is also constructed with low-binding materials;\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003eFeasibility of collecting the filtrate - it is flexible to choose either the retentate or the filtrate to keep;\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003eOptimized designs for better performance:\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003col\u003e\n\u003cli\u003eBuilt-in dead stop pocket at the filter unit bottom - no risk of dryout during spin;\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003eVertical position of the filter membrane - less clogging\/fouling issue;\u003cbr\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cul\u003e\n\u003cli\u003eLess processing time, less buffers, and less mess compared to dialysis;\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003eFlexible throughput - it is easy to perform multiple samples in parallel or to scale up with the same sample;\u003c\/li\u003e\n\u003cli\u003eFree of detectable DNase\/RNase contamination.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eApplications:\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eConcentration, desalting of proteins, enzymes, antibodies, DNA\/RNA and other macromolecules\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003eRapid removal of small contaminants (salts, dyes, detergent, and additives)\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003ePost-modification removal of labeled amino acids and nucleotides, or other unincorporated labels\u003cbr\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eSample pre-treatment prior to HPLC\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eDeproteinization\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eRecovery of biomolecules from cell culture supernatants and cell lysates\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eConcentrating virus from cell culture supernatants\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003e\u003cbr\u003e\u003c\/span\u003e\u003c\/h4\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eTechnical Notes\u003c\/span\u003e\u003c\/h4\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003cp\u003e\u003cstrong\u003e1. Typical retention of protein markers\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cstrong\u003eMarker (conc.)\u003c\/strong\u003e\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003eMW\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003eMWCO\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003eSpin time (min)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003e% Retention\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBSA (1.0 mg\/mL)\u003c\/td\u003e\n\u003ctd\u003e67 kDa\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e30 K\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e30\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u0026gt; 80 %\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBSA (1.0 mg\/mL)\u003c\/td\u003e\n\u003ctd\u003e67 kDa\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e50 K\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e10\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u0026gt; 80 %\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCytochrome C (0.25mg\/mL)\u003c\/td\u003e\n\u003ctd\u003e12.4 kDa\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e50 K\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e10\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u0026lt; 5 %\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eIgG (1.0 mg\/mL)\u003c\/td\u003e\n\u003ctd\u003e156 kDa\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e100 K\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u0026gt; 80%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eOvalbumin (1.0 mg\/mL)\u003c\/td\u003e\n\u003ctd\u003e45 kDa\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e100 K\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e20\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u0026lt; 12%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cb\u003e\u003ci\u003eSingle use only. For research use only. Not intended for any human or animal therapeutic or diagnostic uses.\u003c\/i\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"290\" valign=\"top\"\u003e\u003cstrong\u003eMaximum sample capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e12 mL with a swinging-bucket rotor\u003cbr\u003e\u003c\/p\u003e\n10 mL with a Fixed-angle rotor (Min. rotor angle is 25°)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eMaterial of construction\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003eScrew cap: High-density Polyethylene (HDPE)\u003c\/p\u003e\n\u003cp\u003eSealing gasket: Polyethylene (PE)\u003c\/p\u003e\n\u003cp\u003eFiltrate receiver tube: Polypropylene (PP)\u003c\/p\u003e\n\u003cp\u003eFilter unit: Methyl methacrylate-Butadiene-Styrene (MBS)\u003c\/p\u003e\nUltrafiltration membrane: Polyethersulfone (PES)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eActive ultrafiltration membrane area\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003e5.48 cm\u003csup\u003e2\u003c\/sup\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eProduct dimension\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003eAssembled unit height: 119 mm\u003c\/p\u003e\n\u003cp\u003eAssembled unit O.D.: 29.3 mm\u003c\/p\u003e\n\u003cp\u003eFilter unit height: 72.0 mm\u003c\/p\u003e\nFilter unit O.D. : 29.5 mm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eWetted part\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eNo\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDNase\/RNase\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eNo detectable DNase\/RNase contamination\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eMaxi. centrifugation speed\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003eSwinging-bucket: 4,000xg\u003c\/p\u003e\nFixed angle: 5,000xg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eWorking temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e-20°C to 50°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eChemical stability\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003eStable in most commonly used reagents for protein and nucleic acid applications\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eChemical incompatibility\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003eAcetic Acid (≥ 25%)\u003c\/p\u003e\n\u003cp\u003eAcetonitrile (≥ 30%)\u003c\/p\u003e\n\u003cp\u003eAliphatic \u0026amp; aromatic esters\u003c\/p\u003e\n\u003cp\u003eAmines\u003c\/p\u003e\n\u003cp\u003eAmmonium hydroxide (≥ 5%)\u003c\/p\u003e\n\u003cp\u003eAromatic \u0026amp; chlorinated hydrocarbons\u003c\/p\u003e\n\u003cp\u003eButyl acetate (≥ 40%)\u003c\/p\u003e\n\u003cp\u003eDimethyl acetamide (DMAC) (≥ 30%)\u003c\/p\u003e\n\u003cp\u003eEthers\u003c\/p\u003e\n\u003cp\u003eHydrochloric acid (≥ 0.5 N at 50°C)\u003c\/p\u003e\n\u003cp\u003eIsopropyl Alcohol (≥ 25%)\u003c\/p\u003e\n\u003cp\u003eKetone\u003c\/p\u003e\n\u003cp\u003eMethylene chloride (≥ 1%)\u003c\/p\u003e\n\u003cp\u003eMethyl ethyl ketone (≥ 1%)\u003c\/p\u003e\n\u003cp\u003ePhosphoric acid (≥ 1 N)\u003c\/p\u003e\n\u003cp\u003eSodium deoxycholate (≥ 5%)\u003c\/p\u003e\n\u003cp\u003eSodium hydroxide (≥ 0.5N at 50°C)\u003c\/p\u003e\n\u003cp\u003eSodium hypochlorite (≥ 0.04%)\u003c\/p\u003e\n\u003cp\u003eTetrahydrofuran (≥ 5%)\u003c\/p\u003e\nToluene (≥ 1%)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSterility\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eNon-sterile\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eAutoclavability\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eNo\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eQuality assurance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eManufactured in accordance with ISO 13485 : 2016 \u0026amp; ISO 9001 : 2015 quality management systems.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003ePackage size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e24 spin units per pack.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eRoom temperature. Keep dry.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eApplication note\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003eSingle use only.\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eFor research use only. Not intended for any human or animal therapeutic or diagnostic uses.\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"10 K","offer_id":39263984582699,"sku":"17-0291-10","price":247.0,"currency_code":"USD","in_stock":true},{"title":"30 K","offer_id":39263984615467,"sku":"17-0291-30","price":247.0,"currency_code":"USD","in_stock":true},{"title":"50 K","offer_id":39263984648235,"sku":"17-0291-50","price":247.0,"currency_code":"USD","in_stock":true},{"title":"100 K","offer_id":39263984681003,"sku":"17-0291-00","price":247.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/MWCO_10K.png?v=1622336560"},{"product_id":"proteindex-anti-dykddddk-agarose-4-fast-flow","title":"PROTEINDEX™ Anti-DYKDDDDK Agarose 4 Fast Flow","description":"\u003cp\u003e\u003cstrong\u003ePROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e\u003c\/strong\u003e™ Anti-DYKDDDDK Agarose 4 Fast Flow is an affinity chromatography medium for the purification of proteins that contain the peptide sequence DYKDDDDK (N-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-C). The ligand covalently immobilized on the matrix support is a recombinant mouse monoclonal IgG1 species that binds the same epitope as Sigma's Anti-FLAG® M2 Antibody, and it is highly specific for the peptide DYKDDDDK in a calcium-independent manner. Commonly known as the FLAG® epitope, the amino acid sequence DYKDDDDK has been used as an affinity tag to fuse at the N-terminal, C-terminal, or an internal location of a recombinant protein.\u003c\/p\u003e\n\u003cp\u003eFor protein purification, the ProteIndex™ Anti-DYKDDDDK Agarose 4FF resin is added to the sample. Following capture of the DYKDDDDK-tagged protein, the unbound and non-specifically bound proteins can be washed away. The bound target protein can then be eluted either with the competitive peptide under mild conditions or with the acidic glycine-HCl buffer.  \u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eBecause the binding between the epitope and the antibody can occur under the native conditions, any proteins or other macromolecules that are able to interact with the tagged fusion protein will also be captured during the binding step. Therefore, the anti-DYKDDDDK 4FF affinity resin can be used in co-immunoprecipitation to analyze the protein complex that the target fusion protein is associated with.\u003c\/p\u003e\n\u003cp\u003eThe base matrix of ProteIndex™ Anti-DYKDDDDK Agarose 4FF is highly cross-linked 4% agarose, a robust support offering low background and high physical stability for chromatography procedures at various scales. The affinity resin is recommended for use in gravity\/batch applications, spin purification columns, or cartridges for FPLC instruments.  \u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eProduct Highlights\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eHigh selectivity for high purity (\u0026gt;90%)\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003e\u0026gt; 3 mg DYKDDDDK-tagged protein (50 kDa)\/mL medium\u003c\/li\u003e\n\u003cli\u003eStable coupling of ligand, low leakage; can be re-used to purify the same protein (3~5 repeated uses when acid elution is employed)\u003c\/li\u003e\n\u003cli\u003eVersatile - ready for gravity\/batch, spin columns, and FPLC applications\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003e Technical Notes\u003c\/span\u003e\u003c\/h4\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e \u003cstrong\u003eA.\u003c\/strong\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003eB.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cdiv style=\"text-align: left;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Anti-DYKDDDDK_4FF_peptide_elution_730c0dc2-7077-4c4d-b82a-83fef6e52ed4_480x480.png?v=1622772785\" alt=\"\"\u003e\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cdiv style=\"text-align: left;\"\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Anti-DYKDDDDK_4FF_glycin_elution_e2c690ae-2c1d-4d94-b884-abdfb05a1c35_480x480.png?v=1622772895\"\u003e\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003cstrong\u003eFigure 1. \u003c\/strong\u003e\u003cb\u003ePurification performance of ProteIndex™ Anti-\u003c\/b\u003e\u003cb\u003eDYKDDDDK \u003c\/b\u003e\u003cb\u003e4\u003c\/b\u003e\u003cb\u003eFF affinity resins.\u003c\/b\u003e\u003c\/p\u003e\n\u003cp\u003eWhole cell lysate containing a DYKDDDDK-tagged fusion protein (~ 50 kDa) was loaded on the column packed with the anti-DYKDDDDK 4FF affinity resin. The column was washed with Binding\/Wash Buffer (50 mM Tris，0.15 M NaCl，pH7.4). The target DYKDDDDK-tagged protein was partially eluted with 2 column volumes of 3x DYKDDDDK peptide (0.1 mg\/mL, A) or the glycine-HCl buffer (B).\u003c\/p\u003e\n\u003cp\u003eShown in the SDS-PAGE gel images, from left to right fractions are: lysate (CL), flow-through (FT), eluate (E), and the supernatant of the boiled resin in the SDS-sample buffer (B).\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eThis product can be packed into column for FPLC on automated systems. If you are interested in the pre-packed cartridge formats, talk to \u003ca href=\"mailto:cust.support@marvelgent.com\"\u003eour customer support\u003c\/a\u003e for further assistance.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"200\"\u003e\u003cstrong\u003eLigand\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMouse monoclonal anti-DYKDDDDK IgG1, calcium-independent\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBinding capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e≥ 3 mg DYKDDDDK-tagged protein (~ 50 kDa) per mL medium\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003ePurity \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e≥ 90%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMatrix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e4% highly cross-linked agarose supplied as 50% slurry\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eParticle size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e45 μm - 165 μm with the average particle size being 90 μm\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaximum pressure\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.3 MPa (3 bar) \u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eRecommended flow rate\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cspan data-mce-fragment=\"1\" class=\"ng-binding ng-scope\" ng-bind-html=\"main.bindDemiliter($last, value + ' ' + feature.unitSymbol, '; ')\" ng-repeat=\"value in feature.values\"\u003e30 - 250\u003c\/span\u003e cm\/hour\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003epH stability\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003e3-12\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage buffer\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1x PBS with 0.02% NaN\u003csub data-mce-fragment=\"1\"\u003e3\u003c\/sub\u003e\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProduct is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e   \u003cimg style=\"float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Keep-refrigerated_95fa1867-1f0d-47e6-823a-8a0008076e21_small.png?12332456762384261517\" alt=\"Keep refrigerated\"\u003e   \u003cimg style=\"float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Do-not-freeze_2_small.png?12332456762384261517\" alt=\"Do not freeze\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"2 mL","offer_id":39336067137579,"sku":"18-0270-002","price":451.0,"currency_code":"USD","in_stock":true},{"title":"10 mL","offer_id":39336067170347,"sku":"18-0270-010","price":1668.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/18-0270Anti-DYKDDDDK4FF_3.png?v=1623432010"},{"product_id":"proteindex-streptavidin-agarose-6-fast-flow","title":"PROTEINDEX™ Streptavidin Agarose 6 Fast Flow","description":"\u003cp\u003e\u003cstrong\u003eThe ProteIndex\u003csup\u003eTM\u003c\/sup\u003e Streptavidin Agarose 6FF \u003c\/strong\u003eprovides a simple, rapid, and reliable method to purify the biotin and biotinylated substances. The streptavidin ligand is a recombinant version of the native protein that is originated from \u003cem\u003eStreptomyces avidinii\u003c\/em\u003e, and has an extraordinarily high affinity for biotin. The immobilized streptavidin on the highly cross-linked 6% agarose matrix support can therefore be used for affinity chromatography purification of biotin and biotinylated substances. Due to strong interaction between streptavidin and biotin, elution of biotin and the biotinylated substance usually requires harsh denaturing conditions. Streptavidin also binds to 2-iminobiotin and iminobiotinylated substances, in a pH-dependent fashion. The affinity interaction between the streptavidin and iminobiotin is strong when the pH is above 9.0 and decreases dramatically as the pH is lowered. Therefore, the iminobiotinylated substance can be captured onto the immobilized streptavidin at pH 9.5 – 11.0 and can be eluted at pH 4.0.\u003c\/p\u003e\n\u003cp\u003eAlternatively, the immobilized streptavidin on the matrix support can be used to purify biomolecules that are able to interact and form complexes with a biotinalyted biomolecules. For example, an antigen can be purified via the immunoreactive complex with its specific, biotinylated antibody captured onto the Streptavidin Agarose 6FF resin, and the antigen can be eluted when it dissociates from the complex.\u003c\/p\u003e\n\u003cp\u003eThe base matrix of Streptavidin Agarose 6FF is highly cross-linked 6% agarose, providing excellent flow properties, and high mechanical stability. The resin is well suited for batch\/gravity purification, centrifugation procedure, or FPLC process on automated systems. \u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eProduct Highlights\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eHigh selectivity for high purity\u003c\/li\u003e\n\u003cli\u003eHigh capacity: binding \u0026gt; 75 μg (300 nmol) Biotin \/ ml of settled resin\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003eSuited for batch, gravity, spin, and automated systems.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003e Technical Notes\u003c\/span\u003e\u003c\/h4\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Streptavidin_6FF_purification4_480x480.png?v=1621190683\" alt=\"Streptavidin Agarose 6FF resin for purification of Biotinylated protein\" data-mce-fragment=\"1\"\u003e \u003c\/strong\u003e \u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFigure 1. Purification performance of Streptavidin 6FF resins.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ci\u003eE.coli \u003c\/i\u003elysate containing an iminobiotinylated protein (~ 45 kDa) was 1: 5 diluted in Binding Buffer (50 mM \u003cspan data-mce-fragment=\"1\"\u003e(NH\u003csub data-mce-fragment=\"1\"\u003e4\u003c\/sub\u003e)\u003csub data-mce-fragment=\"1\"\u003e2\u003c\/sub\u003eCO\u003csub data-mce-fragment=\"1\"\u003e3\u003c\/sub\u003e\u003c\/span\u003e, 0.5 M NaCl, pH10.0) and then was loaded on 0.5 mL packed Streptavidin 6FF resins. After washes with the Binding Buffer, bound material was eluted in Elution Buffer (50 mM \u003cspan data-mce-fragment=\"1\"\u003eNH\u003csub data-mce-fragment=\"1\"\u003e4\u003c\/sub\u003eAc\u003c\/span\u003e, 0.5 M NaCl, pH 4.0 ). The resulted resin was then incubated with SDS sample buffer at 95°C for 10 minutes. Fractions were analyzed by SDS-PAGE.\u003c\/p\u003e\n\u003cp\u003eLanes from left to right: Molecular Weight markers (MW), Cell lysate (CL), Flow-through (FL), and Eluate (E), and the Supernatant of boiled resin in SDS sample buffer (B).  \u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"200\"\u003e\u003cstrong\u003eLigand\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eRecombinant Streptavidin expressed in \u003cem data-mce-fragment=\"1\"\u003eE.coli\u003c\/em\u003e\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eBinding capacity \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u0026gt; 50 μg (200 nmol) Biotin \/ ml of settled resin\u003c\/p\u003e\n\u003cp\u003e\u0026gt; 6 mg Biotinylated serum albumin \/ ml of settled resin\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMatrix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e6% highly cross-linked agarose supplied as 50% slurry\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eParticle size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e45 μm - 165 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaximum pressure\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.3 MPa (3 bar, 43 psi)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eRecommended flow rate\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003e300 - 500 cm\/hour\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003epH stability\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003epH3 - 10 (short term) and pH4 - 9 (long term)\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage buffer\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e20% ethanol as preservative\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProduct is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e   \u003cimg style=\"float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Keep-refrigerated_95fa1867-1f0d-47e6-823a-8a0008076e21_small.png?12332456762384261517\" alt=\"Keep refrigerated\"\u003e   \u003cimg style=\"float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Do-not-freeze_2_small.png?12332456762384261517\" alt=\"Do not freeze\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"5 mL","offer_id":39336998895659,"sku":"11-0216-005","price":228.0,"currency_code":"USD","in_stock":true},{"title":"50 mL","offer_id":39336998961195,"sku":"11-0216-050","price":1716.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/11-0216Streptavidin6FF_2.png?v=1623426798"}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/collections\/Collection_DNAisolation-2_v2_nobg.png?v=1458742166","url":"https:\/\/marvelgent.com\/collections\/protein-purification-isolation\/frit-pore-size_50-m-frit+fractionation-specificity_immunoglubolin.oembed","provider":"Marvelgent Biosciences","version":"1.0","type":"link"}