{"title":"Recombinant Protein A resins for antibody purification","description":"\u003cp\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Homepage_ProteinAt.png?v=1493426040\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\nNative Protein A contains five high affinity IgG binding domains and many other domains with unknown functions. The recombinant Protein A coupled to the agarose resin is produced in \u003ci\u003eE. coli\u003c\/i\u003e and contains five high affinity IgG binding domains with other non-essential domains removed to reduce nonspecific binding. Essentially the same as the native Protein A, rProtein A is capable of binding with high affinity to the Fc region from IgG of several species including human and rabbit.","products":[{"product_id":"proteindex-rprotein-a-agarose","title":"PROTEINDEX™ rProtein A Agarose, Settled Resin","description":"\u003cp\u003e\u003cstrong\u003ePROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e\u003c\/strong\u003e™ rProtein A Agarose 4 Fast Flow is an affinity chromatography medium designed for easy, one-step purification of immunoglobulins, notably IgG isotypes, as well as Fc region-containing fusion proteins.\u003c\/p\u003e\n\u003cp\u003eProtein A, a bacterial cell wall protein isolated from \u003cem\u003eStaphylococcus aureus\u003c\/em\u003e, binds to the Fc region of most immunoglobulins through interactions with the heavy chain. Native Protein A contains five high affinity IgG binding domains and many other domains with unknown functions.\u003c\/p\u003e\n\u003cp\u003eThe recombinant Protein A is a genetically engineered protein that contains only the five high affinity IgG binding domains. The non-essential domains were removed to minimize nonspecific binding. The recombinant Protein A is produced in \u003ci\u003eE. coli, \u003c\/i\u003eand it essentially binds to immunoglobulins with the same degree of affinity as the native Protein A. rProtein A is capable of binding with high affinity to the Fc region from IgG of several species including human and rabbit.\u003c\/p\u003e\n\u003cp\u003eEnabled by proprietary ProteIndex™ technology, immobilization of recombinant Protein A on 4% agarose resins was very stable and was optimized for high yield in laboratory preparations and scale-up applications.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eProduct Highlights\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eHigh capacity, \u0026gt; 40 mg human IgG \/ mL of settled resins\u003c\/li\u003e\n\u003cli\u003eHigh selectivity for high purity\u003c\/li\u003e\n\u003cli\u003eMinimal ligand leakage, can be reused\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"5%\"\u003e\u003c\/td\u003e\n\u003ctd width=\"35%\"\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/ProteinA4FF_purifying_mAscites_fluid_3_0d442212-1ce9-4a05-8106-1ec2848dd3cb.png?v=1508439312\"\u003e\u003c\/td\u003e\n\u003ctd valign=\"bottom\"\u003e\n\u003cp\u003e\u003cstrong\u003eHigh purity and high capacity of rProtein A 4FF resin. \u003c\/strong\u003e2 mL mouse ascites fluid was loaded on the column containing 0.5 mL settled rProtein A 4 FF resin. Bound immunoglobulin species were eluted with 100 mM Glycine, pH 3.0. Fractions were analyzed by SDS-PAGE.\u003c\/p\u003e\n\u003cp\u003eLanes: (1) Raw ascites fluid; (2) Flow-through; (3) Elution. Note that, heavy and light chains of IgG were mostly absent from the flow-through, indicating that most immunoglobulins were bound to the column.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"200\"\u003e\u003cstrong\u003eLigand\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eRecombinant protein A produced in \u003cem\u003eE.coli\u003c\/em\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBinding capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;40 mg human IgG\/mL medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMatrix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e4% agarose supplied as 50% slurry\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eParticle size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e45 μm - 165 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaximum pressure\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.1 MPa (1 bar)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eChemical stability\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eAll commonly used aqueous buffers, including 6 M guanidine-HCl and 8 M urea.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eRecommended flow rate\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eGravity flow, 100 - 300 cm\/hour\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003epH stability\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003eLong term: pH3 - 9;\u003c\/p\u003e\n\u003cp\u003eShort term: pH2 - 10\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage buffer\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e20% ethanol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProduct is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e   \u003cimg style=\"float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Keep-refrigerated_95fa1867-1f0d-47e6-823a-8a0008076e21_small.png?12332456762384261517\" alt=\"Keep refrigerated\"\u003e   \u003cimg style=\"float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Do-not-freeze_2_small.png?12332456762384261517\" alt=\"Do not freeze\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eDocuments\u003c\/h5\u003e\n\u003ch4\u003eTechnical Note\u003c\/h4\u003e\n\u003cp\u003e🗎    \u003ca rel=\"noopener noreferrer\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Table_Binding_characteristics_of_immunoglobulin-binding_proteins.pdf?6231220717744860621\" title=\"Comparison of binding characteristics of immunoglobulin-binding proteins\" target=\"_blank\"\u003eComparison of Binding Characteristics of Immunoglobulin-Binding Proteins\u003c\/a\u003e\u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"5 mL","offer_id":14587446980,"sku":"11-0202-005","price":159.0,"currency_code":"USD","in_stock":true},{"title":"25 mL","offer_id":14587447044,"sku":"11-0202-025","price":638.0,"currency_code":"USD","in_stock":true},{"title":"100 mL","offer_id":14587447108,"sku":"11-0202-100","price":1716.0,"currency_code":"USD","in_stock":true},{"title":"200 mL","offer_id":5934033633319,"sku":"11-0202-200","price":3190.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/11-0202_ProteinA.png?v=1508438549"},{"product_id":"proteindex-rprotein-a-agarose-4ff","title":"PROTEINDEX™ rProtein A Agarose 4 Fast Flow","description":"\u003cp\u003e\u003cstrong\u003ePROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e\u003c\/strong\u003e™ rProtein A Agarose 4 Fast Flow is an affinity chromatography medium designed for easy, one-step purification of immunoglobulins, notably IgG isotypes, as well as Fc region-containing fusion proteins.\u003c\/p\u003e\n\u003cp\u003eProtein A, a bacterial cell wall protein isolated from \u003cem\u003eStaphylococcus aureus\u003c\/em\u003e, binds to the Fc region of most immunoglobulins through interactions with the heavy chain. Native Protein A contains five high affinity IgG binding domains and many other domains with unknown functions.\u003c\/p\u003e\n\u003cp\u003eThe recombinant Protein A is a genetically engineered protein that contains only the five high affinity IgG binding domains. The non-essential domains were removed to minimize nonspecific binding. The recombinant Protein A is produced in \u003ci\u003eE. coli, \u003c\/i\u003eand it essentially binds to immunoglobulins with the same degree of affinity as the native Protein A. rProtein A is capable of binding with high affinity to the Fc region from IgG of several species including human and rabbit.\u003c\/p\u003e\n\u003cp\u003eEnabled by proprietary ProteIndex™ technology, immobilization of recombinant Protein A on 4% highly cross-linked agarose resins was very stable and was optimized for high yield in laboratory preparations and scale-up applications.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eProduct Highlights\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eHigh capacity, \u0026gt; 40 mg human IgG \/ mL of settled resins\u003c\/li\u003e\n\u003cli\u003eHigh selectivity for high purity\u003c\/li\u003e\n\u003cli\u003eMechanically stable matrix support, can be used in spin (at up to 1000 g) and automated applications\u003c\/li\u003e\n\u003cli\u003eMinimal ligand leakage, can be reused\u003c\/li\u003e\n\u003cli\u003eVersatile, ready-to-use with convenient formats\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"5%\"\u003e\u003c\/td\u003e\n\u003ctd width=\"35%\"\u003e \u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/ProteinA4FF_purifying_mAscites_fluid_3_0d442212-1ce9-4a05-8106-1ec2848dd3cb.png?v=1508439312\"\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"bottom\"\u003e\n\u003cp\u003e\u003cstrong\u003eHigh purity and high capacity of rProtein A 4FF resin. \u003c\/strong\u003e2 mL mouse ascites fluid was loaded on the column containing 0.5 mL settled rProtein A 4 FF resin. Bound immunoglobulin species were eluted with 100 mM Glycine, pH 3.0. Fractions were analyzed by SDS-PAGE.\u003c\/p\u003e\n\u003cp\u003eLanes: (1) Raw ascites fluid; (2) Flow-through; (3) Elution. Note that, heavy and light chains of IgG were mostly absent from the flow-through, indicating that most immunoglobulins were bound to the column.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"200\"\u003e\u003cstrong\u003eLigand\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eRecombinant protein A with Ig binding domains\u003cem\u003e\u003cbr\u003e\u003c\/em\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBinding capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;40 mg human IgG\/mL medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMatrix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eCross-linked 4% agarose supplied as a 50% slurry\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eParticle size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e45 μm - 165 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaximum pressure\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.3 MPa (3 bar)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eChemical stability\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eAll commonly used aqueous buffers, including 6 M guanidine-HCl and 8 M urea.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eRecommended flow rate\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eGravity flow and medium pressure, 30 - 300 cm\/hour\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003epH stability\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003eLong term: pH 2 - 9;\u003c\/p\u003e\n\u003cp\u003eShort term: pH 2 - 11\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage buffer\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e20% ethanol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProduct is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e   \u003cimg style=\"float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Keep-refrigerated_95fa1867-1f0d-47e6-823a-8a0008076e21_small.png?12332456762384261517\" alt=\"Keep refrigerated\"\u003e   \u003cimg style=\"float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Do-not-freeze_2_small.png?12332456762384261517\" alt=\"Do not freeze\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eDocuments\u003c\/h5\u003e\n\u003ch4\u003eTechnical Note\u003c\/h4\u003e\n\u003cp\u003e🗎    \u003ca rel=\"noopener noreferrer\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Table_Binding_characteristics_of_immunoglobulin-binding_proteins.pdf?6231220717744860621\" title=\"Comparison of binding characteristics of immunoglobulin-binding proteins\" target=\"_blank\"\u003eComparison of Binding Characteristics of Immunoglobulin-Binding Proteins\u003c\/a\u003e\u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"5 mL","offer_id":14675386436,"sku":"11-0203-005","price":379.0,"currency_code":"USD","in_stock":true},{"title":"25 mL","offer_id":14675386500,"sku":"11-0203-025","price":1461.0,"currency_code":"USD","in_stock":true},{"title":"100 mL","offer_id":5933537263655,"sku":"11-0203-100","price":3025.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/11-0203_ProteinA4FF.png?v=1513270183"},{"product_id":"proteindex-rprotein-a-agarose-4ff-prepacked-cartridge","title":"PROTEINDEX™ rProtein A Agarose 4FF, Prepacked Cartridge","description":"\u003cp\u003e\u003cstrong\u003ePROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e\u003c\/strong\u003e™ rProtein A Agarose 4 Fast Flow is an affinity chromatography medium designed for easy, one-step purification of immunoglobulins, notably IgG isotypes, as well as Fc region-containing fusion proteins.\u003c\/p\u003e\n\u003cp\u003eProtein A, a bacterial cell wall protein isolated from \u003cem\u003eStaphylococcus aureus\u003c\/em\u003e, binds to the Fc region of most immunoglobulins through interactions with the heavy chain. Native Protein A contains five high affinity IgG binding domains and many other domains with unknown functions. The recombinant Protein A coupled to the agarose resin is produced in \u003ci\u003eE. coli\u003c\/i\u003e and contains five high affinity IgG binding domains with other non-essential domains removed to reduce nonspecific binding. Essentially the same as the native Protein A, rProtein A is capable of binding with high affinity to the Fc region from IgG of several species including human and rabbit.\u003c\/p\u003e\n\u003cp\u003eEnabled by proprietary ProteIndex™ technology, immobilization of recombinant Protein A on 4% highly cross-linked agarose resins was very stable and was optimized for high yield in laboratory preparations and scale-up applications.\u003c\/p\u003e\n\u003cp\u003eThe Prepacked Cartridge has standard interface and can be adapted to most commonly used chromatography systems, such as ÄKTA systems.\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eValue bundle with a \u003c\/span\u003e\u003ca rel=\"noopener\" title=\"Connector Luer Female \/ 10-32 Male in PEEK\" href=\"https:\/\/marvelgent.com\/products\/connector-1-16-luer-female-10-32-male-peek\" target=\"_blank\"\u003eLuer Female\/10-32 Male Connector in PEEK\u003c\/a\u003e\u003cspan\u003e (Cat# 20-0315) is now available for all pack sizes, providing convenient operation of the pre-packed column with a syringe at an excellent value.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eProduct Highlights\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eHigh capacity, \u0026gt; 40 mg human IgG \/ mL of settled resins\u003c\/li\u003e\n\u003cli\u003eHigh selectivity for high purity\u003c\/li\u003e\n\u003cli\u003eMinimal ligand leakage, can be reused\u003c\/li\u003e\n\u003cli\u003eReady-to-use, compatible with standard automated systems\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"5%\"\u003e\u003c\/td\u003e\n\u003ctd width=\"35%\"\u003e \u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/ProteinA4FF_purifying_mAscites_fluid_3_0d442212-1ce9-4a05-8106-1ec2848dd3cb.png?v=1508439312\"\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"bottom\"\u003e\n\u003cp\u003e\u003cstrong\u003eHigh purity and high capacity of rProtein A 4FF resin. \u003c\/strong\u003e2 mL mouse ascites fluid was loaded on the column containing 0.5 mL settled rProtein A 4 FF resin. Bound immunoglobulin species were eluted with 100 mM Glycine, pH 3.0. Fractions were analyzed by SDS-PAGE.\u003c\/p\u003e\n\u003cp\u003eLanes: (1) Raw ascites fluid; (2) Flow-through; (3) Elution. Note that, heavy and light chains of IgG were mostly absent from the flow-through, indicating that most immunoglobulins were bound to the column.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"200\"\u003e\u003cstrong\u003eLigand\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eRecombinant protein A produced in \u003cem\u003eE.coli\u003c\/em\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBinding capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;40 mg human IgG\/mL medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMatrix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eHighly cross-linked 4% agarose\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eParticle size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e45 μm - 165 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaximum pressure\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.3 MPa (3 bar)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eChemical stability\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eAll commonly used aqueous buffers, including 6 M guanidine-HCl and 8 M urea.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eRecommended flow rate\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eGravity flow and medium pressure, 30 - 300 cm\/hour\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003epH stability\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003eLong term: pH 3 - 10;\u003c\/p\u003e\n\u003cp\u003eShort term: pH 2 - 11.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage buffer\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e20% ethanol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProduct is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e   \u003cimg style=\"float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Keep-refrigerated_95fa1867-1f0d-47e6-823a-8a0008076e21_small.png?12332456762384261517\" alt=\"Keep refrigerated\"\u003e   \u003cimg style=\"float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Do-not-freeze_2_small.png?12332456762384261517\" alt=\"Do not freeze\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eDocuments\u003c\/h5\u003e\n\u003ch4\u003eTechnical Note\u003c\/h4\u003e\n\u003cp\u003e🗎    \u003ca rel=\"noopener noreferrer\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Table_Binding_characteristics_of_immunoglobulin-binding_proteins.pdf?6231220717744860621\" title=\"Comparison of binding characteristics of immunoglobulin-binding proteins\" target=\"_blank\"\u003eComparison of Binding Characteristics of Immunoglobulin-Binding Proteins\u003c\/a\u003e\u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"5x 1 mL Columns","offer_id":14710543492,"sku":"11-0204-5x1ML","price":572.0,"currency_code":"USD","in_stock":true},{"title":"5x 1 mL Columns with 1 Connector","offer_id":41405719740459,"sku":"11-0204-5x1MLB","price":599.0,"currency_code":"USD","in_stock":true},{"title":"1x 5 mL Column","offer_id":14710543556,"sku":"11-0204-1x5ML","price":555.0,"currency_code":"USD","in_stock":true},{"title":"1x 5 mL Column with 1 Connector","offer_id":41405719773227,"sku":"11-0204-1x5MLB","price":583.0,"currency_code":"USD","in_stock":true},{"title":"5x 5 mL Columns","offer_id":14710543620,"sku":"11-0204-5x5ML","price":2348.0,"currency_code":"USD","in_stock":true},{"title":"5x 5 mL Columns with 1 Connector","offer_id":41405719805995,"sku":"11-0204-5x5MLB","price":2376.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/Marvelgent_pre-packed_cartridges.png?v=1459891864"},{"product_id":"proteindex-rprotein-a-alkali-tolerant-agarose-4ff","title":"PROTEINDEX™ Alkali-Tolerant Protein A Agarose 4 Fast Flow","description":"\u003cp\u003e\u003cstrong\u003ePROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e\u003c\/strong\u003e™ rProtein A Agarose 4 Fast Flow is a proprietary affinity chromatography medium designed for easy, one-step purification of immunoglobulins, notably monoclonal antibodies, from samples of larger volumes.\u003c\/p\u003e\n\u003cp\u003eAlkali-tolerant protein A is engineered by gene recombination technology on the base of native protein A. Our Alkali-Tolerant Protein A Agarose 4FF retains ≥95% capacity after 200 times repeated clean-in-place (CIP) cycles with 0.1M NaOH, and approximately 80% of the initial capacity with 0.5M NaOH after 100 times repeated CIP cycles. Alkali-Tolerant Protein A is immobilized onto the cross-linked 4% agarose resin with an orientation that is optimal for immunoglobulin binding. The level of leakage of the ligand during elution is very low.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"200\"\u003e\u003cstrong\u003eLigand\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eRecombinant alkali-tolerant protein A produced in \u003cem\u003eE.coli\u003c\/em\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBinding capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;40 mg rabbit IgG\/mL medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMatrix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eCross-linked 4% agarose supplied as a 50% slurry\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eParticle size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e45 μm - 165 μm, with an average of ~ 90 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaximum pressure\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.3 MPa (3 bar)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eRecommended flow rate\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eGravity flow and medium pressure, 30 - 300 cm\/hour\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eChemical stability\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eAll commonly used aqueous buffers and reagents in the process of antibody purification, including 6 M guanidine-HCl and 8 M urea.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eCleaning-in-place (CIP)\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.1 M ~ 0.5 M NaOH\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage buffer\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e20% ethanol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProduct is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e   \u003cimg alt=\"Keep refrigerated\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Keep-refrigerated_95fa1867-1f0d-47e6-823a-8a0008076e21_small.png?12332456762384261517\" style=\"float: none;\"\u003e   \u003cimg alt=\"Do not freeze\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Do-not-freeze_2_small.png?12332456762384261517\" style=\"float: none;\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eDocuments\u003c\/h5\u003e\n\u003ch4\u003eTechnical Note\u003c\/h4\u003e\n\u003cp\u003e🗎    \u003ca title=\"Comparison of binding characteristics of immunoglobulin-binding proteins\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Table_Binding_characteristics_of_immunoglobulin-binding_proteins.pdf?6231220717744860621\" target=\"_blank\"\u003eComparison of Binding Characteristics of Immunoglobulin-Binding Proteins\u003c\/a\u003e\u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"10 mL","offer_id":16984774020,"sku":"11-0211-010","price":1207.0,"currency_code":"USD","in_stock":true},{"title":"50 mL","offer_id":16984774084,"sku":"11-0211-050","price":3638.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/Protein_At_agarose4FF.png?v=1490749970"},{"product_id":"proteindex-protein-a-alkali-tolerant-agarose-4ff-prepacked-cartridge","title":"PROTEINDEX™ Alkali-Tolerant Protein A Agarose 4FF, Prepacked Cartridge","description":"\u003cp\u003e\u003cstrong\u003ePROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e\u003c\/strong\u003e™ rProtein A Agarose 4 Fast Flow is a proprietary affinity chromatography medium designed for easy, one-step purification of immunoglobulins, notably monoclonal antibodies, from samples of larger volumes.\u003c\/p\u003e\n\u003cp\u003eAlkali-tolerant protein A is engineered by gene recombination technology on the base of native protein A. Our Alkali-Tolerant Protein A Agarose 4FF retains ≥95% capacity after 200 times repeated clean-in-place (CIP) cycles with 0.1M NaOH, and approximately 80% of the initial capacity with 0.5M NaOH after 100 times repeated CIP cycles. Alkali-Tolerant Protein A is immobilized onto the cross-linked 4% agarose resin with an orientation that is optimal for immunoglobulin binding. The level of leakage of the ligand during elution is very low.\u003c\/p\u003e\n\u003cp\u003eThe Prepacked Cartridge has standard interface and can be adapted to most commonly used chromatography systems, such as ÄKTA systems.\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eValue bundle with a \u003c\/span\u003e\u003ca href=\"https:\/\/marvelgent.com\/products\/connector-1-16-luer-female-10-32-male-peek\" title=\"Connector Luer Female \/ 10-32 Male in PEEK\" rel=\"noopener\" target=\"_blank\"\u003eLuer Female\/10-32 Male Connector in PEEK\u003c\/a\u003e\u003cspan\u003e (Cat# 20-0315) is now available for all pack sizes, providing convenient operation of the pre-packed column with a syringe at an excellent value.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"200\"\u003e\u003cstrong\u003eLigand\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eRecombinant alkali-tolerant protein A produced in \u003cem\u003eE.coli\u003c\/em\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBinding capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;40 mg rabbit IgG\/mL medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMatrix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eCross-linked 4% agarose\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eParticle size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e45 μm - 165 μm, with an average size of ~ 90 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaximum pressure\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.3 MPa (3 bar)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eRecommended flow rate\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eGravity flow and medium pressure, 30 - 300 cm\/hour\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eChemical stability\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eAll commonly used aqueous buffers and reagents in the process of antibody purification, including 6 M guanidine-HCl and 8 M urea.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eCleaning-in-place (CIP)\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.1 M ~ 0.5 M NaOH\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage buffer\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e20% ethanol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProduct is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e   \u003cimg style=\"float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Keep-refrigerated_95fa1867-1f0d-47e6-823a-8a0008076e21_small.png?12332456762384261517\" alt=\"Keep refrigerated\"\u003e   \u003cimg style=\"float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Do-not-freeze_2_small.png?12332456762384261517\" alt=\"Do not freeze\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eDocuments\u003c\/h5\u003e\n\u003ch4\u003eTechnical Note\u003c\/h4\u003e\n\u003cp\u003e🗎    \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Table_Binding_characteristics_of_immunoglobulin-binding_proteins.pdf?6231220717744860621\" title=\"Comparison of binding characteristics of immunoglobulin-binding proteins\" target=\"_blank\"\u003eComparison of Binding Characteristics of Immunoglobulin-Binding Proteins\u003c\/a\u003e\u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"5x 1 mL Columns","offer_id":16990807748,"sku":"11-0212-5x1ML","price":643.0,"currency_code":"USD","in_stock":true},{"title":"5x 1 mL Columns with 1 Connector","offer_id":41405722329131,"sku":"11-0212-5x1MLB","price":671.0,"currency_code":"USD","in_stock":true},{"title":"1x 5 mL Column","offer_id":16990807812,"sku":"11-0212-1x5ML","price":610.0,"currency_code":"USD","in_stock":true},{"title":"1x 5 mL Column with 1 Connector","offer_id":41405722361899,"sku":"11-0212-1x5MLB","price":638.0,"currency_code":"USD","in_stock":true},{"title":"5x 5 mL Columns","offer_id":16990807876,"sku":"11-0212-5x5ML","price":2637.0,"currency_code":"USD","in_stock":true},{"title":"5x 5 mL Columns with 1 Connector","offer_id":41405722394667,"sku":"11-0212-5x5MLB","price":2665.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/ProteinAt_column2.png?v=1493134475"},{"product_id":"proteindex-rprotein-a-agarose-gravity-flow-column","title":"PROTEINDEX™ Recombinant Protein A Prepacked Gravity Flow Columns, 1 mL, Pack of 5 Columns","description":"\u003cp\u003e\u003cstrong\u003ePROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e\u003c\/strong\u003e™ recombinant Protein A Agarose Gravity Flow Column is ready-to-use affinity chromatography column for purification of immunoglobulins, notably IgG isotypes, as well as Fc region-containing fusion proteins.\u003c\/p\u003e\n\u003cp\u003eProtein A, a bacterial cell wall protein isolated from \u003cem\u003eStaphylococcus aureus\u003c\/em\u003e, binds to the Fc region of most immunoglobulins through interactions with the heavy chain. Native Protein A contains five high affinity IgG binding domains and many other domains with unknown functions.\u003c\/p\u003e\n\u003cp\u003eThe recombinant Protein A is a genetically engineered protein that contains only the five high affinity IgG binding domains. The non-essential domains were removed to minimize nonspecific binding. The recombinant Protein A is produced in \u003ci\u003eE. coli, \u003c\/i\u003eand it essentially binds to immunoglobulins with the same degree of affinity as the native Protein A. rProtein A is capable of binding with high affinity to the Fc region from IgG of several species including human and rabbit.\u003c\/p\u003e\n\u003cp\u003eEnabled by proprietary ProteIndex™ technology, immobilization of recombinant Protein A on 4% agarose resins was very stable and was optimized for high yield in laboratory preparations and scale-up applications.\u003c\/p\u003e\n\u003cp\u003eEach gravity column contains 1 mL of recombinant Protein A agarose resin, and is individually wrapped.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eProduct Highlights\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eHigh capacity, \u0026gt; 40 mg human IgG per column\u003c\/li\u003e\n\u003cli\u003eHigh selectivity for high purity\u003c\/li\u003e\n\u003cli\u003eMinimal ligand leakage, can be reused\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"5%\"\u003e\u003c\/td\u003e\n\u003ctd width=\"35%\"\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/ProteinA4FF_purifying_mAscites_fluid_3_0d442212-1ce9-4a05-8106-1ec2848dd3cb.png?v=1508439312\"\u003e\u003c\/td\u003e\n\u003ctd valign=\"bottom\"\u003e\n\u003cp\u003e\u003cstrong\u003eHigh purity and high capacity of recombinant Protein A ligand. \u003c\/strong\u003e2 mL mouse ascites fluid was loaded on the column containing 0.5 mL settled rProtein A 4 FF resin. Bound immunoglobulin species were eluted with 100 mM Glycine, pH 3.0. Fractions were analyzed by SDS-PAGE.\u003c\/p\u003e\n\u003cp\u003eLanes: (1) Raw ascites fluid; (2) Flow-through; (3) Elution. Note that, heavy and light chains of IgG were mostly absent from the flow-through, indicating that most immunoglobulins were bound to the column.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"200\"\u003e\u003cstrong\u003eProduct package size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e5 columns\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"200\"\u003e\u003cstrong\u003eColumn volume\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1 mL settled Protein A resins\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eLigand\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eRecombinant protein A produced in \u003cem\u003eE.coli\u003c\/em\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBinding capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;40 mg rabbit IgG\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMatrix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e4% agarose\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eParticle size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e45 μm - 165 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMaximum pressure\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.1 MPa (1 bar)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eChemical stability\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eAll commonly used aqueous buffers, including 6 M guanidine-HCl and 8 M urea.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eRecommended flow rate\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eGravity flow, 100 - 300 cm\/hour\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003epH stability\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eLong term: pH 3 - 9; \u003cbr\u003eShort term: pH 2 - 10\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage buffer\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e20% ethanol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eColumn material\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003epolypropylene (body)\u003cbr\u003eLow density polyethylene (top snap cap and bottom tip cap)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eFrit\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003ePolyethylene, with an average pore size of 30 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProduct is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e   \u003cimg style=\"float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Keep-refrigerated_95fa1867-1f0d-47e6-823a-8a0008076e21_small.png?12332456762384261517\" alt=\"Keep refrigerated\"\u003e   \u003cimg style=\"float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Do-not-freeze_2_small.png?12332456762384261517\" alt=\"Do not freeze\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eDocuments\u003c\/h5\u003e\n\u003ch4\u003eTechnical Note\u003c\/h4\u003e\n\u003cp\u003e🗎    \u003ca rel=\"noopener noreferrer\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Table_Binding_characteristics_of_immunoglobulin-binding_proteins.pdf?6231220717744860621\" title=\"Comparison of binding characteristics of immunoglobulin-binding proteins\" target=\"_blank\"\u003eComparison of Binding Characteristics of Immunoglobulin-Binding Proteins\u003c\/a\u003e\u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"5 Columns","offer_id":5915590983719,"sku":"18-0268","price":247.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/ProteinA_gravity_column.png?v=1512006816"},{"product_id":"magindex-rprotein-a-magnetic-agarose-resin","title":"MagINDEX™ rProtein A Magnetic Agarose, Settled Resin","description":"\u003cp\u003e\u003cstrong\u003eMag\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e\u003c\/strong\u003e™ rProtein A Magnetic Agarose is an affinity chromatography medium designed for rapid, easy, one-step purification of immunoglobulins, notably IgG isotypes, as well as Fc region-containing fusion proteins.\u003c\/p\u003e\n\u003cp\u003eProprietary MagIndex\u003cb\u003e™\u003c\/b\u003e technology facilitates encapsulation of the superparamagnetic core in the mechanically stable Agarose matrix, which is the optimal absorbance for high yield protein purification with minimal background.\u003c\/p\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"15%\"\u003e\u003c\/td\u003e\n\u003ctd width=\"70%\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Schematic_composition_of_mag_agarose_resins_862e6d64-9839-4cc9-95d6-b8f9f1b47dcf.png?v=1527170756\" alt=\"\"\u003e\u003c\/td\u003e\n\u003ctd width=\"15%\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003eThe superparamagnetic core has very fast magnetic response in the magnetic field, but no memory after the magnetic force is removed. The agarose-based encapsulation provides a strong shield well protecting the magnetic core in a wide range of reagents that are commonly used in protein purification. Moreover, as an ideal matrix for protein purification, the agarose matrix enables purification of antibodies and Ig fragments with high purity and high capacity, in high throughput screening and various preparative scales.\u003c\/p\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cdiv class=\"video-wrapper\"\u003e\u003ciframe src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/MagIndex_Magnetic_Agarose_Resins-3.mp4?14800512398908436554\"\u003e\u003c\/iframe\u003e\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd width=\"50%\" valign=\"top\"\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cspan style=\"color: #444444;\"\u003e\u003cstrong\u003eSuperparamagnetic core\u003c\/strong\u003e with fast response to magnetic field\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan style=\"color: #444444;\"\u003e\u003cstrong\u003eLow nonspecific binding\u003c\/strong\u003e for assay accuracy\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan style=\"color: #444444;\"\u003e\u003cstrong\u003eHigh density of the Protein A\u003c\/strong\u003e ligand for optimal yield of antibodies and Ig fragments\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan style=\"color: #444444;\"\u003e\u003cstrong\u003eHigh stability\u003c\/strong\u003e in common buffer systems and detergents\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProtein A, a bacterial cell wall protein isolated from \u003cem\u003eStaphylococcus aureus\u003c\/em\u003e, binds to the Fc region of most immunoglobulins through interactions with the heavy chain. Native Protein A contains five high affinity IgG binding domains and many other domains with unknown functions.\u003c\/p\u003e\n\u003cp\u003eThe recombinant Protein A is a genetically engineered protein that contains only the five high affinity IgG binding domains. The non-essential domains were removed to minimize nonspecific binding. The recombinant Protein A is produced in \u003ci\u003eE. coli, \u003c\/i\u003eand it essentially binds to immunoglobulins with the same degree of affinity as the native Protein A. rProtein A is capable of binding with high affinity to the Fc region from IgG of several species including human and rabbit.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eProduct Highlights\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eHigh capacity, \u0026gt; 10 mg rabbit IgG \/ mL of settled resins\u003c\/li\u003e\n\u003cli\u003eHigh selectivity for high purity\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eApplications\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eQuick isolation of antibodies from sera and ascites fluids\u003c\/li\u003e\n\u003cli\u003ePurification of scFc fragment from cell cultures\u003c\/li\u003e\n\u003cli\u003eDepletion of unwanted antibodies and fragments from sample\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eTech Note\u003c\/span\u003e\u003c\/h4\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"25%\" valign=\"top\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/MagIndex_Protein_A-4.png?14389587024720716232\" alt=\"\"\u003e\u003c\/td\u003e\n\u003ctd valign=\"bottom\"\u003e\n\u003cp\u003e\u003cstrong\u003eRecombinant scFc protein was isolated from cell culture with MagIndex rProtein A Magnetic Agarose resins. \u003c\/strong\u003eBound the scFc protein was eluted with 100 mM Glycine, pH 3.0, denoted by the blue arrow. Fractions were analyzed by SDS-PAGE.\u003c\/p\u003e\n\u003cp\u003eLanes: (L) Culture supernatant; (FT) Flow-through; (E) Elutions 1 and 2.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSample:\u003c\/strong\u003e 10 mL of culture supernatant containing a scFc fragment (20 µg\/mL)\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eResin:\u003c\/strong\u003e 0.2 mL settle resins of MagIndex Protein A magnetic agarose\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBinding:\u003c\/strong\u003e 45 min at room temperature\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eWashing:\u003c\/strong\u003e 4 mL binding buffer\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eElution:\u003c\/strong\u003e Incubate in 100 mM Glycine, pH 3.0 for 10 min. Repeat 3 times\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"200\"\u003e\u003cstrong\u003eLigand\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eRecombinant protein A containing only Ig binding domains\u003cem\u003e\u003cbr\u003e\u003c\/em\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBinding capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;10 mg rabbit IgG\/mL medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eMatrix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eCross-linked agarose beaded particles containing the superparamagnetic core, supplied as a 20% slurry\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eParticle size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e30 μm - 100 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003epH stability\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003eLong term: pH 2 - 9;\u003c\/p\u003e\n\u003cp\u003eShort term: pH 2 - 11\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage buffer\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003ePBS containing 0.01% Tween-20 and 0.02% NaN\u003csub\u003e3\u003c\/sub\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProduct is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e   \u003cimg alt=\"Keep refrigerated\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Keep-refrigerated_95fa1867-1f0d-47e6-823a-8a0008076e21_small.png?12332456762384261517\" style=\"float: none;\"\u003e   \u003cimg alt=\"Do not freeze\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Do-not-freeze_2_small.png?12332456762384261517\" style=\"float: none;\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eDocuments\u003c\/h5\u003e\n\u003ch4\u003eTechnical Note\u003c\/h4\u003e\n\u003cp\u003e🗎    \u003ca title=\"Comparison of binding characteristics of immunoglobulin-binding proteins\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Table_Binding_characteristics_of_immunoglobulin-binding_proteins.pdf?6231220717744860621\" rel=\"noopener noreferrer\" target=\"_blank\"\u003eComparison of Binding Characteristics of Immunoglobulin-Binding Proteins\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"2 mL","offer_id":6599808221223,"sku":"11-0271-002","price":225.0,"currency_code":"USD","in_stock":true},{"title":"5 mL","offer_id":6600507916327,"sku":"11-0271-005","price":491.0,"currency_code":"USD","in_stock":true},{"title":"10 mL","offer_id":6600507949095,"sku":"11-0271-010","price":710.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/MagIndex_Protein_A.png?v=1525138379"},{"product_id":"magindex-rprotein-a-g-magnetic-agarose-settled-resin","title":"MagINDEX™ rProtein A\/G Magnetic Agarose, Settled Resin","description":"\u003cp\u003e\u003cstrong\u003eMag\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e\u003c\/strong\u003e™ rProtein A\/G Magnetic Agarose is an affinity chromatography medium designed for rapid, easy, one-step purification of a wide range of immunoglobulins, as well as Fc region-containing fusion proteins.\u003c\/p\u003e\n\u003cp\u003eProprietary MagIndex\u003cb\u003e™\u003c\/b\u003e technology facilitates encapsulation of the superparamagnetic core in the mechanically stable Agarose matrix, which is the optimal absorbance for high yield protein purification with minimal background.\u003c\/p\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"15%\"\u003e\u003c\/td\u003e\n\u003ctd width=\"70%\"\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Schematic_composition_of_mag_agarose_resins_862e6d64-9839-4cc9-95d6-b8f9f1b47dcf.png?v=1527170756\"\u003e\u003c\/td\u003e\n\u003ctd width=\"15%\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003eThe superparamagnetic core has very fast magnetic response in the magnetic field, but no memory after the magnetic force is removed. The agarose-based encapsulation provides a strong shield well protecting the magnetic core in a wide range of reagents that are commonly used in protein purification. Moreover, as an ideal matrix for protein purification, the agarose matrix enables purification of antibodies and Ig fragments with high purity and high capacity, in high throughput screening and various preparative scales.\u003c\/p\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cdiv class=\"video-wrapper\"\u003e\u003ciframe src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/MagIndex_Magnetic_Agarose_Resins-3.mp4?14800512398908436554\"\u003e\u003c\/iframe\u003e\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"50%\"\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cspan style=\"color: #444444;\"\u003e\u003cstrong\u003eSuperparamagnetic core\u003c\/strong\u003e with fast response to magnetic field\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan style=\"color: #444444;\"\u003e\u003cstrong\u003eLow nonspecific binding\u003c\/strong\u003e for assay accuracy\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan style=\"color: #444444;\"\u003e\u003cstrong\u003eHigh density of the Protein A\/G\u003c\/strong\u003e ligand for optimal yield of antibodies and Ig fragments\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan style=\"color: #444444;\"\u003e\u003cstrong\u003eHigh stability\u003c\/strong\u003e in common buffer systems and detergents\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProtein A, a bacterial cell wall protein isolated from \u003cem\u003eStaphylococcus aureus\u003c\/em\u003e, binds to the Fc region of most immunoglobulins through interactions with the heavy chain. Native Protein A contains five high affinity IgG binding domains and many other domains with unknown functions. While the native protein G has 3 IgG binding domains as well as sites for albumin and cell-surface binding, recombinant protein G has only Ig recognition domains but no other non-specific sequences. \u003c\/p\u003e\n\u003cp\u003eRecombinant Protein A\/G is a recombinant chimeric protein that is composed of the IgG binding domains of both Protein A and Protein G. rProtein A\/G contains five binding domains from Protein A and two from Protein G. The binding of rProtein A\/G is less pH-dependent than Protein A, but otherwise has the additive properties of Protein A and G. Therefore, rProtein A\/G is ideal for binding the broadest range of IgG subclasses from rabbit, mouse, human and other mammalian samples.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eProduct Highlights\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eHigh capacity, \u0026gt; 10 mg rabbit IgG \/ mL of settled resins\u003c\/li\u003e\n\u003cli\u003eHigh selectivity for high purity\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003e\u003cspan style=\"color: #3d85c6;\"\u003eApplications\u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eQuick isolation of antibodies from sera and ascites fluids\u003c\/li\u003e\n\u003cli\u003ePurification of recombinant antibody or scFc fragment from cell cultures\u003c\/li\u003e\n\u003cli\u003eDepletion of unwanted antibodies and fragments from sample\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"200\"\u003e\u003cstrong\u003eLigand\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eRecombinant chimeric protein A\/G containing only Ig binding domains\u003cem\u003e\u003cbr\u003e\u003c\/em\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBinding capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;10 mg rabbit IgG\/mL medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003eMatrix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eCross-linked agarose beaded particles containing the superparamagnetic core, supplied as a 20% slurry\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eParticle size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e30 μm - 100 μm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\u003cstrong\u003epH stability\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003eLong term: pH 2 - 9;\u003c\/p\u003e\n\u003cp\u003eShort term: pH 2 - 11\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage buffer\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003ePBS containing 0.01% Tween-20 and 0.02% NaN\u003csub\u003e3\u003c\/sub\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProduct is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e   \u003cimg style=\"float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Keep-refrigerated_95fa1867-1f0d-47e6-823a-8a0008076e21_small.png?12332456762384261517\" alt=\"Keep refrigerated\"\u003e   \u003cimg style=\"float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Do-not-freeze_2_small.png?12332456762384261517\" alt=\"Do not freeze\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eDocuments\u003c\/h5\u003e\n\u003ch4\u003eTechnical Note\u003c\/h4\u003e\n\u003cp\u003e🗎    \u003ca rel=\"noopener noreferrer\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Table_Binding_characteristics_of_immunoglobulin-binding_proteins.pdf?6231220717744860621\" title=\"Comparison of binding characteristics of immunoglobulin-binding proteins\" target=\"_blank\"\u003eComparison of Binding Characteristics of Immunoglobulin-Binding Proteins\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"2 mL","offer_id":12406185754667,"sku":"11-0273-002","price":376.0,"currency_code":"USD","in_stock":true},{"title":"5 mL","offer_id":12406185787435,"sku":"11-0273-005","price":826.0,"currency_code":"USD","in_stock":true},{"title":"10 mL","offer_id":12406185820203,"sku":"11-0273-010","price":1207.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/MagIndex_Protein_A_d9a5f606-cc94-42ef-9a01-9a1543202424.png?v=1528207075"},{"product_id":"proteindex-alkali-tolerant-protein-a-plus","title":"PROTEINDEX™ Alkali-Tolerant Protein A Plus Agarose Resin, High Capacity","description":"\u003cp\u003e\u003cstrong\u003ePROTE\u003cspan style=\"color: #ee8012;\"\u003eINDEX\u003c\/span\u003e\u003c\/strong\u003e™ rProtein A Plus resin is an agarose-based, proprietary affinity chromatography medium designed for easy, one-step purification of immunoglobulins, notably monoclonal antibodies. \u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eAlkali-tolerant protein A is engineered by gene recombination technology on the base of native protein A.  The Alkali-Tolerant Protein A Plus resin retains ≥95% capacity after 200 times repeated clean-in-place (CIP) cycles with 0.1M NaOH, and approximately 80% of the initial capacity with 0.5M NaOH after 100 times repeated CIP cycles. Alkali-Tolerant Protein A is immobilized onto the resin with an orientation that is optimal for immunoglobulin binding. The level of leakage of the ligand during elution is very low.\u003c\/p\u003e\n\u003cp\u003eThe resin is highly cross-linked agarose based resin. The bead size is smaller and is optimized for higher binding capacity. The matrix is more rigid and offers higher mechanical stability for faster flow rates between 50 - 500 cm\/hour.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eProduct Details\u003c\/h5\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 36.6906%;\" width=\"200\"\u003e\u003cstrong\u003eLigand\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 63.1295%;\"\u003eRecombinant alkali-tolerant protein A produced in \u003cem\u003eE.coli\u003c\/em\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 36.6906%;\"\u003e\u003cstrong\u003eBinding capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 63.1295%;\"\u003e\u0026gt;60 mg rabbit IgG\/mL medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 36.6906%;\"\u003e\u003cstrong\u003eMatrix\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 63.1295%;\"\u003eHighly cross-linked agarose based resin, supplied as a 50% slurry\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 36.6906%;\"\u003e\u003cstrong\u003eParticle size, d\u003csub\u003e50V\u003c\/sub\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 63.1295%;\"\u003e~ 85 μm \u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 36.6906%;\"\u003e\u003cstrong\u003eMaximum pressure\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 63.1295%;\"\u003e0.3 MPa (3 bar)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 36.6906%;\"\u003e\u003cstrong\u003eRecommended flow rate\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 63.1295%;\"\u003e100 - 500 cm\/hour\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 36.6906%;\" valign=\"top\"\u003e\u003cstrong\u003eChemical stability\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 63.1295%;\"\u003eAll commonly used aqueous buffers and reagents in the process of antibody purification, including 6 M guanidine-HCl and 8 M urea.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 36.6906%;\" valign=\"top\"\u003e\u003cstrong\u003epH Stability\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 63.1295%;\"\u003e\n\u003cp\u003eLong term: pH 3 - 12\u003c\/p\u003e\n\u003cp\u003eShort term: pH 2 - 14\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 36.6906%;\"\u003e\u003cstrong\u003eCleaning-in-place (CIP)\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 63.1295%;\"\u003e0.1 M ~ 0.5 M NaOH\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 36.6906%;\"\u003e\u003cstrong\u003eStorage temperature\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 63.1295%;\"\u003e2 - 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 36.6906%;\"\u003e\u003cstrong\u003eStorage buffer\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 63.1295%;\"\u003e20% ethanol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eProduct is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e   \u003cimg style=\"float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Keep-refrigerated_95fa1867-1f0d-47e6-823a-8a0008076e21_small.png?12332456762384261517\" alt=\"Keep refrigerated\"\u003e   \u003cimg style=\"float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/icon-Do-not-freeze_2_small.png?12332456762384261517\" alt=\"Do not freeze\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eDocuments\u003c\/h5\u003e\n\u003ch4\u003eTechnical Note\u003c\/h4\u003e\n\u003cp\u003e🗎    \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/files\/Table_Binding_characteristics_of_immunoglobulin-binding_proteins.pdf?6231220717744860621\" title=\"Comparison of binding characteristics of immunoglobulin-binding proteins\" target=\"_blank\"\u003eComparison of Binding Characteristics of Immunoglobulin-Binding Proteins\u003c\/a\u003e\u003c\/p\u003e","brand":"Marvelgent Biosciences Inc","offers":[{"title":"10 mL","offer_id":40634725892139,"sku":"11-0297-010","price":1438.0,"currency_code":"USD","in_stock":true},{"title":"50 mL","offer_id":40634725924907,"sku":"11-0297-050","price":3748.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0985\/1250\/products\/11-0211ProteinAt.png?v=1678811997"}],"url":"https:\/\/marvelgent.com\/collections\/recombinant-protein-a-resins-for-antibody-purification\/separation-method_gravity-flow+fractionation-specificity_immunoglubolin.oembed","provider":"Marvelgent Biosciences","version":"1.0","type":"link"}