PROTEINDEX™ HiBond™ Ni-NTA Agarose
Catalog NO.
High binding capacity, single-step gravity chromatography resin for routine purification of His tagged fusion proteins. ~40 mg histidine-tagged protein/mL medium. ... Read more
Description
PROTEINDEX™ HiBond™ Ni-NTA Agarose utilizes 4% agarose as the matrix support, and is functionalized with the chelate ligand Nitilotriacetic Acid (NTA), which is charged with nickel ions (Ni2+) to form a very stable octahedral structure with the nickel ion in the center. The structure of Ni-NTA complex is compatible with solutions that contain certain concentration of reducing agents, denaturing agents, detergents and other additives.
PROTEINDEX™ HiBond™ Ni-NTA Agarose can be used to purify 6xHis-tagged proteins expressed in various hosts, such as E.coli., yeast, insect cells and mammalian cells.
Product Highlights
- Suited for routine purification of histidine-tagged proteins
- High yield (typically > 40 mg protein /mL resins)
- Low leakage of nickel ions. Can be re-used for up to 8 preps*
*Note: No significant decrease in yield after 8 repetitive uses of resin when recommended buffers are used. Refer to Product Details for compatible reagents.
Applications
- Manual batch or gravity flow purification
- Routine His tag protein purification for structural biology and functional assays
- Protein-protein and protein-nucleic acid interaction studies
Tech Notes
One-step purification using ProteIndex™ HiBond™ Ni-NTA Agarose 6 Fast Flow resins
Product Details
Ligand | Nickel | ||||||||||
Binding capacity | >40 mg 6xHis-tagged protein/mL medium | ||||||||||
Matrix | 4% agarose supplied as 50% slurry | ||||||||||
Particle size | 45 μm - 165 μm | ||||||||||
Maximum pressure |
0.1 MPa (1 bar) | ||||||||||
Recommended flow rate |
Gravity flow, <75 cm/hour | ||||||||||
Chemical compatibility |
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Storage temperature |
2 - 8°C | ||||||||||
Storage buffer |
1x PBS containing 20% ethanol |
Product is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.
Contact Us
Marvelgent Biosciences Inc.
Head Office
116 Will Drive, Canton, MA 02021, USA
Telephone
1.888.330.6623
Fax
1.888.330.6623
Emails
Ordering support: order.support@marvelgent.com
Technical support: cust.support@marvelgent.com
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