Chromatography applications require the use of column that can retain the solid phase and allow the mobile liquid phase to pass. The columns made of glass are found to be hard to clean and may be a source of impurities, and besides, they are expensive and very easy to chip (or break!).
For many gravity purification needs, such as affinity purifications that involve just simply binding, washing and elution, plastic disposable columns perform perfectly so that they have become popular alternatives to glass columns.
Marvelgent Disposable Gravity Flow Columns provide cost-effective, convenient, versatile options for routine protein separation by gravity flow. The columns are suitable for affinity purification, desalting, isolation of antibody and fusion protein.
Each column set comes with one polypropylene column body, fritted with a matching frit, a top plug, a bottom cap, and an extra frit, which can be used to double fritting to provide extra support or to place on top of the settled resins to prevent drying.
|Click and enlarge video to view||
The frits are made of UHMW-PE, and are dedicatedly optimized for smooth aqueous flow driven by gravity while holding the samples bound on the particle matrix. The frits deliver a typical flow rate within the range of 1 ~ 4 mL/min by gravity.
In general, the frit that has larger pore size will allow faster flow through. However, if the pore size is too big for the absorbent particles that are being used in the application, the particles are likely to be trapped in the frit, subsequently resulting in blockage of the flow through. Therefore, depending on the particle size of absorbent, we recommend to choose the frit that has a pore size smaller than half of the average particle size of the absorbent. For example, if the resins have an average size of 100 μm, a 50 μm frit can be used in the purification; whereas if the resins have an average size of 50 μm, a 20 μm frit works better.
We provide a variety of columns to fit your application needs:
|Frit Pore Size|
|Column Size||I.D. (mm)||20 μm||50 μm||80 μm|
|3 mL||9.0||Cat# 11-0258|
|6 mL||13.0||Cat# 11-0259||
|12 mL||15.8||Cat# 11-0257||
|30 mL||22.5||Cat# 11-0260||Cat# 12-0278||
|60 mL||26.6||Cat# 11-0282||Cat# 12-0279||
|300 mL||49.5||Cat# 11-0261||Cat# 12-0280||
If you are looking for frits having a different pore size, or you want to a custom-made column with specific configurations, speak with our Customer Support Team to see how we can help!
For fast, general-purpose purification by gravity flow or low pressure:
- Affinity chromatography of protein or other macromolecules
- Immunodepletion of immune reactive complexes
- Size-exclusion chromatography
For research use only. Not intended for any human or animal therapeutic or diagnostic uses.