ProteoSure™ Endoproteinase GluC, V8 Protease, Sequencing Grade
Highly purified V8 Endoproteinase GluC is suited for peptide sequencing/mapping and protein identification by peptide mass fingerprinting or MS/MS spectral matching. ... Read more
Description
Staphylococcus aureus Protease V8 (Endoproteinase GluC) is a serine proteinase that selectively cleaves peptide bonds C-terminal to glutamic acid residues. It also cleaves at aspartic acid residues. The Optimum pH is 8.0 ~ 8.5. Its enzyme inhibitors include Phosphoric acid diisopropyl ester fluoride (DFP), alpha-2-macroglobulin, and Nα-Tosyl-L-lysine chloromethyl ketone (TLCK).
Endoproteinase Glu-C is active in the presence of 0.5% SDS and retains 80% activity after 12 hours at 25°C. It also remains its 95% enzymatic activity after 2 hours in 2 M urea.
Endoproteinase Glu-C is suitable for peptide sequencing and mapping applications, and protein identification by peptide mass fingerprinting or MS/MS spectral matching.
Instruction to use
Recommended condition for digestion: pH 8.0~8.5.
Product Details
| CAS # | 66676-43-5 |
| Enzyme Commission # | 3.4.21.19 |
| Specific activity |
Approx. 20 U/mg at 25°C with Z-Phe-Leu-Glu-4-nitranilide as the substrate; Approx. 500 U/mg at 37°C with casein as the substrate. |
| Storage | -20°C |
| Intended use | For research only. Not intended for any human or animal diagnostic or therapeutic use. |
Documents
Product Information Sheet
🗎 Recombinant V8 Protease, Endoproteinase GluC
Technical Note
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