Efficient, single-step chromatography purification of Glutathione S-Transferase (GST) tagged fusion proteins with high yield and purity. Suitable for other glutathione transferases and glutathione binding proteins. ... Read more
PROTEINDEX™ Glutathione Agarose 4FF is an affinity chromatography medium developed for easy, one-step purification of recombinant glutathione S-transferase (GST) fusion proteins and other glutathione binding proteins expressed in E. coli, insect and mammalian cells. The rigid, highly cross-linked support is suitable for purification scale-up applications.
Our proprietary ProteIndex™ coupling technology ensures stable immobilization of active ligand and minimal ligand leakage in a wide range of purification buffer conditions. The resins can be reused multiple times to purify the same target protein.
- High selectivity for high purity
- > 10 mg GST/mL settled resins
- Stable coupling of ligand, low leakage; can be re-used to purify the same protein
- Versatile, ready-to-use with convenient formats
Purification performance of Glutathione 4FF resins.
50 mL of E.coli lysate containing a GST-tagged fusion protein was loaded on 0.5 mL packed Glutathione 4FF resins at 0.5 mL/mL. The column was then washed with 10 mL of PBS. The target GST-tagged protein was eluted with 7.5 mL of elution buffer (50 mM Tris-HCl, 10 mM GSH, pH 8.0). Fractions of lysate, flow-through, and eluate were analysed by SDS-PAGE. Lanes: (1) Raw cell lysate; (2) Flow-through; (3) Eluate.
|Ligand||Glutathione coupled with a 12-atom linker|
||120 - 320 μmol Glutathione/mL medium|
|Binding capacity||>10 mg GST (26 kDa)/mL medium|
|Matrix||4% highly cross-linked agarose supplied as 50% slurry|
|Particle size||45 μm - 165 μm|
||0.3 MPa (3 bar, 43 psi)|
|Recommended flow rate
||300 - 500 cm/hour|
||2 - 8°C|
||20% ethanol as preservative|
Product is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.
Marvelgent Biosciences Inc.
116 Will Drive, Canton, MA 02021, USA
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