Ready-to-use Protein A column for purification of immunoglobulins by gravity flow. One column yields >40 mg human IgG. Economical prep for high purity. ... Read more
PROTEINDEX™ recombinant Protein A Agarose Gravity Flow Column is ready-to-use affinity chromatography column for purification of immunoglobulins, notably IgG isotypes, as well as Fc region-containing fusion proteins.
Protein A, a bacterial cell wall protein isolated from Staphylococcus aureus, binds to the Fc region of most immunoglobulins through interactions with the heavy chain. Native Protein A contains five high affinity IgG binding domains and many other domains with unknown functions.
The recombinant Protein A is a genetically engineered protein that contains only the five high affinity IgG binding domains. The non-essential domains were removed to minimize nonspecific binding. The recombinant Protein A is produced in E. coli, and it essentially binds to immunoglobulins with the same degree of affinity as the native Protein A. rProtein A is capable of binding with high affinity to the Fc region from IgG of several species including human and rabbit.
Enabled by proprietary ProteIndex™ technology, immobilization of recombinant Protein A on 4% agarose resins was very stable and was optimized for high yield in laboratory preparations and scale-up applications.
Each gravity column contains 1 mL of recombinant Protein A agarose resin, and is individually wrapped.
- High capacity, > 40 mg human IgG per column
- High selectivity for high purity
- Minimal ligand leakage, can be reused
High purity and high capacity of recombinant Protein A ligand. 2 mL mouse ascites fluid was loaded on the column containing 0.5 mL settled rProtein A 4 FF resin. Bound immunoglobulin species were eluted with 100 mM Glycine, pH 3.0. Fractions were analyzed by SDS-PAGE.
Lanes: (1) Raw ascites fluid; (2) Flow-through; (3) Elution. Note that, heavy and light chains of IgG were mostly absent from the flow-through, indicating that most immunoglobulins were bound to the column.
|Product package size||5 columns|
||1 mL settled Protein A resins|
|Ligand||Recombinant protein A produced in E.coli|
|Binding capacity||>40 mg human IgG|
|Particle size||45 μm - 165 μm|
|Maximum pressure||0.1 MPa (1 bar)|
|Chemical stability||All commonly used aqueous buffers, including 6 M guanidine-HCl and 8 M urea.|
|Recommended flow rate
||Gravity flow, 100 - 300 cm/hour|
|pH stability||Long term: pH 3 - 9;
Short term: pH 2 - 10
||2 - 8°C|
|Column material||polypropylene (body)
Low density polyethylene (top snap cap and bottom tip cap)
|Frit||Polyethylene, with an average pore size of 30 μm|
Product is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.
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