Immobilized-Metal Affinity Chromatography
At Marvelgent, we offer a range of IMAC resins for various purification needs, from fast research laboratory separation to preparative and process scale applications.
Ni-NTA Agarose and Ni-NTA Agarose 6FF resins provide efficient, high yield purification of His-tagged proteins. They are the most commonly used IMAC media.
Ni-Penta™ resins utilize a proprietary pentadentate ligand that contributes not only to high selective capture of His-tagged proteins but also to superior stability of His-tagged protein complex on the matrix surface. This high affinity between the His-tagged protein and the matrix remains uncompromised in a variety of chemical and agents, including EDTA, and DTT. Therefore, Ni-Penta™ resins enable direct capture of target His-tagged proteins in various buffer conditions, including mammalian culture supernatants. In addition, leaching of nickel ions is negligible, even after repet. This protects your precious protein from toxic nickel as well.
Tech Note: Chemical Tolerant Ni-Penta™ Resins for High Selective Purification of His-Tagged Proteins
Co-NTA resins are precharged with the cobalt ion (Co2+), which has higher affinity for the His-tag and therefore results in higher purity of product. The use of cobalt in the purification of His-tagged proteins can also avoid the toxicity resulted from the leakage of nickel as the heavy metal.
Featured Product Collections:
To select the right type of IMAC media for the purification of your precious protein, read our suggestions:
Tech Note: IMAC Resins Selection Guide
PROTEINDEX™ HiBond™ Ni-NTA Agarose 6FF
Catalog NO. 11-0225-010PROTEINDEX™ HiBond™ Ni-NTA Agarose 6FF has been optimized for automated purification applications. It has been widely used in scale-up and preparative purification of histidine-tagged proteins that are expressed in various...
PROTEINDEX™ HiBond™ Ni-NTA Agarose 6FF, Prepacked Cartridge
Catalog NO. 11-0230-5x1MLPROTEINDEX™ HiBond™ Ni-NTA Agarose 6FF has been optimized for automated purification applications. It has been widely used in scale-up and preparative purification of histidine-tagged proteins that are expressed in various...
PROTEINDEX™ PureBind™ Co-NTA Agarose 6 Fast Flow
Catalog NO. 11-0231-010PROTEINDEX™ Co-NTA Agarose 6FF is a cobalt-based immobilized metal ion affinity chromatography (IMAC) medium. It offers greater specificity and therefore enhanced selectivity for polyhistidine-tagged proteins under both native and denaturing...
PROTEINDEX™ PureBind™ Co-NTA Agarose 6 Fast Flow, Prepacked Cartridge
Catalog NO. 11-0253-5x1MLPROTEINDEX™ Co-NTA Agarose 6FF is a cobalt-based immobilized metal ion affinity chromatography (IMAC) medium. It offers greater specificity and therefore enhanced selectivity for polyhistidine-tagged proteins under both native and denaturing...
PROTEINDEX™ Ni-IDA Agarose 6FF
Catalog NO. 11-0222-025PROTEINDEX™ Ni-IDA Agarose 6FF is designed mainly for scaling up purification of histidine-tagged proteins that are expressed in various hosts, such as E.coli, yeast, insect cells, and mammalian cells. Highly...