Immobilized-Metal Affinity Chromatography
At Marvelgent, we offer a range of IMAC resins for various purification needs, from fast research laboratory separation to preparative and process scale applications.
Ni-NTA Agarose and Ni-NTA Agarose 6FF resins provide efficient, high yield purification of His-tagged proteins. They are the most commonly used IMAC media.
Ni-Penta™ resins utilize a proprietary pentadentate ligand that contributes not only to high selective capture of His-tagged proteins but also to superior stability of His-tagged protein complex on the matrix surface. This high affinity between the His-tagged protein and the matrix remains uncompromised in a variety of chemical and agents, including EDTA, and DTT. Therefore, Ni-Penta™ resins enable direct capture of target His-tagged proteins in various buffer conditions, including mammalian culture supernatants. In addition, leaching of nickel ions is negligible, even after repet. This protects your precious protein from toxic nickel as well.
Tech Note: Chemical Tolerant Ni-Penta™ Resins for High Selective Purification of His-Tagged Proteins
Co-NTA resins are precharged with the cobalt ion (Co2+), which has higher affinity for the His-tag and therefore results in higher purity of product. The use of cobalt in the purification of His-tagged proteins can also avoid the toxicity resulted from the leakage of nickel as the heavy metal.
Featured Product Collections:
To select the right type of IMAC media for the purification of your precious protein, read our suggestions:
Tech Note: IMAC Resins Selection Guide
PROTEINDEX™ Ni-Penta™ Agarose, Chemical Stable
Catalog NO. 11-0227-010PROTEINDEX™ Chemical-Tolerant Ni-Penta™ Agarose is a new immobilized metal ion affinity chromatography (IMAC) medium precharged with nickel ions, designed mainly for purification of low abundant proteins or challenging proteins in...
PROTEINDEX™ HiBond™ Ni-NTA Agarose
Catalog NO. 11-0224-010PROTEINDEX™ HiBond™ Ni-NTA Agarose utilizes 4% agarose as the matrix support, and is functionalized with the chelate ligand Nitilotriacetic Acid (NTA), which is charged with nickel ions (Ni2+) to form...
PROTEINDEX™ PureBind™ Co-NTA Agarose
Catalog NO. 11-0252-010PROTEINDEX™ PureBind™ Co-NTA Agarose is a cobalt-based immobilized metal ion affinity chromatography (IMAC) medium. It offers greater specificity and therefore enhanced selectivity for polyhistidine-tagged proteins under both native and denaturing...