HiBond™ Ni-NTA resins for high yield His-tag purification
PROTEINDEX™ HiBond™ Ni-NTA resins offer reproducible high binding capacity, single-step chromatography for routine & scale up purification of polyhistidine tagged fusion proteins. ~40 mg his-tagged protein/mL of medium.
The resins are functionalized with the chelate ligand Nitilotriacetic Acid (NTA), which is a stable complex with nickel ions (Ni2+). The structure of Ni-NTA complex is compatible solutions that contain certain concentration of reducing agents, denaturing agents, detergents and other additives.
PROTEINDEX™ HiBond™ Ni-NTA resins can be used to purify 6xHis-tagged proteins expressed in various hosts, such as E.coli., yeast, insect cells and mammalian cells.
PROTEINDEX™ HiBond™ Ni-NTA Agarose
Catalog NO. 11-0224-010PROTEINDEX™ HiBond™ Ni-NTA Agarose utilizes 4% agarose as the matrix support, and is functionalized with the chelate ligand Nitilotriacetic Acid (NTA), which is charged with nickel ions (Ni2+) to form...