High binding capacity, single-step gravity chromatography resin for routine purification of His tagged fusion proteins. ~40 mg histidine-tagged protein/mL medium. ... Read more
PROTEINDEX™ HiBond™ Ni-NTA Agarose utilizes 4% agarose as the matrix support, and is functionalized with the chelate ligand Nitilotriacetic Acid (NTA), which is charged with nickel ions (Ni2+) to form a very stable octahedral structure with the nickel ion in the center. The structure of Ni-NTA complex is compatible solutions that contain certain concentration of reducing agents, denaturing agents, detergents and other additives.
PROTEINDEX™ HiBond™ Ni-NTA Agarose can be used to purify 6xHis-tagged proteins expressed in various hosts, such as E.coli., yeast, insect cells and mammalian cells.
- Suited for routine purification of histidine-tagged proteins
- High yield (typically > 40 mg protein /mL resins)
- Low leakage of nickel ions. Can be re-used for up to 8 preps*
*Note: No significant decrease in yield after 8 repetitive uses of resin when recommended buffers are used. Refer to Product Details for compatible reagents.
- Manual batch or gravity flow purification
- Routine His tag protein purification for structural biology and functional assays
- Protein-protein and protein-nucleic acid interaction studies
One-step purification using ProteIndex™ HiBond™ Ni-NTA Agarose 6 Fast Flow resins
|Binding capacity||>40 mg 6xHis-tagged protein/mL medium|
|Matrix||4% agarose supplied as 50% slurry|
|Particle size||45 μm - 165 μm|
||0.1 MPa (1 bar)|
|Recommended flow rate
||Gravity flow, <75 cm/hour|
||2 - 8°C|
||1x PBS containing 20% ethanol|
Product is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.
Marvelgent Biosciences Inc.
116 Will Drive, Canton, MA 02021, USA
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