MagINDEX™ Ni NTA Magnetic Agarose, Settled Resin
High binding capacity, single-step chromatography for routine & scale up purification of polyhistidine tagged fusion proteins. ~40 mg his-tagged protein/mL medium. ... Read more
Description
MagIndex™ Ni-NTA Magnetic Agarose Resin is affinity chromatography media designed for one-step, small scale, high capacity purification of polyHistidine-tagged fusion proteins from various expression hosts, such as E.coli, yeast, insect cells and mammalian cells.
The resins are agarose based magnetic microspheres that are functionalized with Nitilotriacetic acid (NTA). The chelate ligand is charged with nickel ions (Ni2+), forming a very stable octahedral structure with the nickel ion in the center. The structure of Ni-NTA complex is compatible with solutions containing certain concentrations of reducing agents, denaturing agents, detergents and other additives.
Product Highlights
- Adaptable to high-throughput His tag purifications
- High yield (typically > 40 mg protein /mL resins)
- Low leakage of nickel ions. Can be re-used for up to 8 preps*
*Note: No significant decrease in yield after 8 repetitive uses of resin when recommended buffers are used. Refer to Product Details for compatible reagents.
Product Details
| Ligand | Nickel | ||||||||||
| Binding capacity | > 40 mg 6xHis-tagged protein/mL medium | ||||||||||
| Matrix | Superparamagnetic agarose beads | ||||||||||
| Particle size | 30 μm - 100 μm | ||||||||||
| Concentration | 20% suspension | ||||||||||
| Chemical compatibility |
|
||||||||||
| Storage temperature |
2 - 8°C | ||||||||||
| Storage buffer |
1x PBS containing 20% ethanol |
Product is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.
Contact Us
Marvelgent Biosciences Inc.
Head Office
303 Wyman St, Waltham, MA 02451, USA
Telephone
1.888.330.6623
Fax
1.888.330.6623
Emails
Ordering support: order.support@marvelgent.com
Technical support: cust.support@marvelgent.com
Careers: career@marvelgent.com
You are welcome to leave a message using our Contact Form. We will get back to you within 24 hours.
We Also Recommend
