Ultra pure Endoproteinase Lys-C contains highly specific enzymatic activity. Suitable for protein structure research, sequencing analysis, and antibody quality control. ... Read more
Endoproteinase Lys-C is a serine protease specific for cleavage of amide and peptide ester bonds at the carboxylic side of lysine and S-aminoethylcysteine residues.
The recombinant Endoproteinase Lys-C is genetically engineered from the Achromobacter lyticus lysyl endoproteinase. It is expressed in E.coli and purified by high pressure liquid chromatography.
The recommended ratio to the sample protein is 1:1000 to 1:20000 (w/w), the optimum pH is pH 7 - 9.5. The enzymatic reaction should be carried out at 30 - 37°C for 2 - 24 hours.
The use of enzyme inhibitors (such as aprotinin, DFP, leupeptin, and TLCK) or protein denaturation with guanidine chloride (GuCl) is not recommended.
Recombinant Lys-C is compatible with the following agents: 0.2% SDS, 1.0% Triton, 6 M Urea, 0.5 M NaCl, and 20% Acetonitrile.
Storage and stability
Store recombinant carboxypeptidase B lyophilized powder in a sealed container at 2-8°C. It is stable for 12 months.
The lyophilized powder can be reconstituted with ddH2O to the working concentration; and the solution is stable at 2-8°C for 7 days.
It is also recommended to dissolve the lyophilized powder in 25 mM Tris-HCl (pH 8.5) and make a stock concentrate solution. Aliquots of the stock solution may be stored at -20°C for up to 6 months. Avoid freezing-and-thawing cycles.
|Enzyme Commission #||188.8.131.52|
|Molecular weight||31 kDa|
||≥ 3.0 AU/mg protein|
|Unit definition||One Amidase Unit (AU) is the amount of enzyme, which produces 1 micromole Ac-Lys-pNA per minute at 25 mM Tris-HCl (pH 9.0) at 37°C.|
||No trypsin, chymotrypsin, carboxypeptidase A, Lys-N, Arg-C or other protease contaminants.|
|Storage||Store in a sealed container at 2 - 8°C. Once reconstituted, it should be stored at -20°C for up to 6 months. Avoid freeze-and-thaw cycles.|
|Intended use||For research only. Not intended for any human or animal diagnostic or therapeutic use.|
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