Custom Chromatography Service

Custom chromatography resin service:

1.  Immobilization of user-defined ligand on the desired solid medium support

We can help you develop chromatography resins conjugated with the desired ligand. When you are looking for a specific ligand to purify your protein on a desired matrix support but nowhere it can be found from the market place, we can help you conjugate the ligand on the support.

Off-the-shelf matrices include:

• Highly-crosslinked 4% agarose (or 4 Fast Flow)
• Highly-crosslinked 6% agarose (or 6 Fast Flow)
• Highly-crosslinked agarose with dextran surface extender  NEW ! Find out more >
  
• Functionalizable agarose-base core encapsulated with molecular size-exclusion shell NEW ! Find out more >
• Magnetic agarose
• Crosslinked dextran
• Magnetic polymer microsphere

We currently support scale-up and production of the customized resin to lot sizes of 300 L.

2.  Optimization of matrix support

When the matrix needs to be optimized for specific application, we can work with you to tailor a solution right for your needs. We help optimize the matrix by controlling the following properties:

a)  Agarose-based resins

• Particle size (with average size of 34 µm, 40 µm, or 90 µm)
• Degree of crosslinking to achieve variable mechanical stability and flow rate limits
  
• Pore size of the matrix
• Option of magnetic property

b)  Polymeric particles

• Polymer material
• Particle size
• Magnetic property

3.  Optimization and immobilization of ligand

The efficiency of chromatographic purification of target proteins also depends on the density and orientation of the ligand, as well as the chemistry being used to immobilize it on the matrix support. We can help you to improve the purification effectiveness and eventually help save your budget.

• Spacer arm length
• Density of ligand
• Orientation of ligand on support

Contact us now to speak with our scientists and see how we can help!

Column packing service