Highly purified V8 Endoproteinase GluC is suited for peptide sequencing/mapping and protein identification by peptide mass fingerprinting or MS/MS spectral matching. ... Read more
Staphylococcus aureus Protease V8 (Endoproteinase GluC) is a serine proteinase that selectively cleaves peptide bonds C-terminal to glutamic acid residues. It also cleaves at aspartic acid residues. The Optimum pH is 8.0 ~ 8.5. Its enzyme inhibitors include Phosphoric acid diisopropyl ester fluoride (DFP), alpha-2-macroglobulin, and Nα-Tosyl-L-lysine chloromethyl ketone (TLCK).
Endoproteinase Glu-C is active in the presence of 0.5% SDS and retains 80% activity after 12 hours at 25°C. It also remains its 95% enzymatic activity after 2 hours in 2 M urea.
Endoproteinase Glu-C is suitable for peptide sequencing and mapping applications, and protein identification by peptide mass fingerprinting or MS/MS spectral matching.
Instruction to use
Recommended condition for digestion: pH 8.0~8.5.
|Enzyme Commission #||126.96.36.199|
Approx. 20 U/mg at 25°C with Z-Phe-Leu-Glu-4-nitranilide as the substrate;
Approx. 500 U/mg at 37°C with casein as the substrate.
|Intended use||For research only. Not intended for any human or animal diagnostic or therapeutic use.|
Product Information Sheet
🗎 Recombinant V8 Protease, Endoproteinase GluC
Marvelgent Biosciences Inc.
116 Will Drive, Canton, MA 02021, USA
Ordering support: firstname.lastname@example.org
Technical support: email@example.com
You are welcome to leave a message using our Contact Form. We will get back to you within 24 hours.
We Also Recommend