PROTEINDEX™ PureBind™ Co-NTA Agarose 6 Fast Flow, Prepacked Cartridge
Ready-to-use columns packed with cobalt-based IMAC medium for enhanced selective purification of His-tagged proteins under native and denaturing conditions. >20mg/mL medium ... Read more
Description
PROTEINDEX™ Co-NTA Agarose 6FF is a cobalt-based immobilized metal ion affinity chromatography (IMAC) medium. It offers greater specificity and therefore enhanced selectivity for polyhistidine-tagged proteins under both native and denaturing conditions, compared to Nickel-charged media. Co-NTA Agarose 6 Fast Flow resins can be used for small-scale and preparative large-scale purification of histidine-tagged recombinant proteins from prokaryotic and eukaryotic expression systems.
Product Highlights
- Ready-to-use, convenient formats: 1 mL and 5 mL
- Mechanical stable, suited for preparative and large scale purification of histidine-tagged proteins
- Higher specificity and selectivity for His-tagged proteins, compared with Nickel-based IMAC. Can be used to purify challenging proteins that are expressed in low levels or when the use of nickel is not desired.
- Suitable for purifying proteins from prokaryotic and eukaryotic expression systems
- Compatible with applications under native and denaturing conditions
- Low cobalt ion leakage.
Product Details
| Ligand | Cobalt ion | ||||||||
| Binding capacity | >20 mg 6XHis-tagged protein/mL medium | ||||||||
| Matrix | Highly cross-linked 6% agarose resins | ||||||||
| Particle size | 45 μm - 165 μm | ||||||||
| Maximum pressure |
0.3 MPa (3 bar) | ||||||||
| Chemical compatibility |
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| Storage temperature |
2 - 8°C | ||||||||
| Storage buffer |
1X PBS containing 20% ethanol |
Product is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.
Contact Us
Marvelgent Biosciences Inc.
Head Office
303 Wyman St, Waltham, MA 02451, USA
Telephone
1.888.330.6623
Fax
1.888.330.6623
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Technical support: cust.support@marvelgent.com
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