PROTEINDEX™ Alkali-Tolerant Protein A Agarose 4FF, Prepacked Cartridge
Proprietary engineered, high capacity, low leakage, alkali-stable rProtein A resin optimized for mAb purification. Stable after 200 CIP cycles with 0.1 M NaOH. ... Read more
Description
PROTEINDEX™ rProtein A Agarose 4 Fast Flow is a proprietary affinity chromatography medium designed for easy, one-step purification of immunoglobulins, notably monoclonal antibodies, from samples of larger volumes.
Alkali-tolerant protein A is engineered by gene recombination technology on the base of native protein A. Our Alkali-Tolerant Protein A Agarose 4FF retains ≥95% capacity after 200 times repeated clean-in-place (CIP) cycles with 0.1M NaOH, and approximately 80% of the initial capacity with 0.5M NaOH after 100 times repeated CIP cycles. Alkali-Tolerant Protein A is immobilized onto the cross-linked 4% agarose resin with an orientation that is optimal for immunoglobulin binding. The level of leakage of the ligand during elution is very low.
The Prepacked Cartridge has standard interface and can be adapted to most commonly used chromatography systems, such as ÄKTA systems.
Value bundle with a Luer Female/10-32 Male Connector in PEEK (Cat# 20-0315) is now available for all pack sizes, providing convenient operation of the pre-packed column with a syringe at an excellent value.
Product Details
| Ligand | Recombinant alkali-tolerant protein A produced in E.coli |
| Binding capacity | >40 mg human IgG/mL medium |
| Matrix | Cross-linked 4% agarose |
| Particle size | 45 μm - 165 μm, with an average size of ~ 90 μm |
| Maximum pressure |
0.3 MPa (3 bar) |
| Recommended flow rate | Gravity flow and medium pressure, 30 - 300 cm/hour |
| Chemical stability | All commonly used aqueous buffers and reagents in the process of antibody purification, including 6 M guanidine-HCl and 8 M urea. |
| Cleaning-in-place (CIP) |
0.1 M ~ 0.5 M NaOH |
| Storage temperature |
2 - 8°C |
| Storage buffer |
20% ethanol |
Product is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.
Documents
Technical Note
🗎 Comparison of Binding Characteristics of Immunoglobulin-Binding Proteins
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Marvelgent Biosciences Inc.
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