ProteoSure™ Recombinant Carboxypeptidase B
Recombinant Carboxypeptidase B is 100% Animal Origin Free, >95% pure, suited for bioprocessing and bioproduction, manufactured in ISO9001 facility. ... Read more
Description
Carboxypeptidase B catalyzes the hydrolysis of basic amino acids lysine, arginine, and histidine at the C-terminal end of polypeptides. The molecular weight is 33.8 kDa, the optimum pH is 8.0, and pI is 6.0. Carboxypeptidase B is competitively inhibited by arginine and lysine. The enzyme is also inhibited by metal chelating agents, e.g., EDTA.
Marvelgent's recombinant Carboxypeptidase B is expressed in E.coli and is purified by high pressure liquid chromatography. No trace contamination with proteases, such as carboxypeptidase A or chymotrypsin, nor protease inhibitors such as PMSF.
Product Highlights
- 100% Animal Origin Free (AOF)
By using of Marvelgent’s recombinant carboxypeptidases B, it helps to avoid contamination with viruses and any other potential adventitious agents that can be found in animal-derived carboxypeptidase B.
- High purity
Recombinant carboxypeptidase B exhibits highly specific enzymatic activity with no contaminating proteases , such as chymotrypsin or carboxypeptidase A that are commonly found in animal-originated enzymes.
- Stability
Provided as sterile lyophilized powder, highly stable during transportation and storage.
Product Details
| CAS # | 9025-24-5 |
| Enzyme Commission # | 3.4.17.2 |
| Molecular weight | 34.5 kDa |
| Origin of species | Rat |
| Source | E.coli |
| Purified by | HPLC |
| Physical form | White to off-white lyophilized powder |
| Additives | Tris salts, NaCl and carbohydrates. |
| Protein content | 35%～60% |
| Specific activity |
≥170 Units/mg protein |
| Unit definition | One unit of carboxypeptidase B activity hydrolyzes one micromole of hippuryl-L-arginine per minute at 25℃, pH 7.65. |
| Purity | > 95% |
| Contaminant activity |
No chymotrypsin, carboxypeptidase A, or other protease contaminants. Less than 10 ppm of recombinant trypsin. |
| Storage | Store in a sealed container at 2 - 8°C. Once reconstituted, it should be stored at -20°C. The product is stable for 24 months. Greater than 90% enzymatic activity retains after 10 repeated freeze-and-thaw cycles. |
| Intended use |
For research or further processing only. Not intended for any human or animal diagnostic or therapeutic use. |
Documents
Product Information Sheet
🗎 Recombinant Carboxypeptidase B, 100% Animal Original Free
Technical Note
Contact Us
Marvelgent Biosciences Inc.
Head Office
303 Wyman St, Waltham, MA 02451, USA
Telephone
1.888.330.6623
Fax
1.888.330.6623
Emails
Ordering support: order.support@marvelgent.com
Technical support: cust.support@marvelgent.com
Careers: career@marvelgent.com
You are welcome to leave a message using our Contact Form. We will get back to you within 24 hours.
We Also Recommend
