ProteoSure™ Recombinant Porcine Trypsin
Recombinant porcine Trypsin is 100% Animal-Origin Free. >99% pure. Activity ≥3800 U/mg. Suitable for bioprocessing. Manufactured in ISO9001 facility. ... Read more
Description
Trypsin is a member of the serine protease family. Trypsin cleaves peptides on the C-terminal end of lysine and arginine amino acid residues. The optimum pH of trypsin lies between 7 - 9. The enzyme is inhibited by serine protease inhibitors (e.g. PMSF) and metal chelating agents (e.g. EDTA).
Recombinant Porcine Trypsin is a genetically engineered protein that is expressed in E. coli and purified by high pressure liquid chromatography. There is no contaminating enzyme activity from carboxypeptidase A or chymotrypsin. No protease inhibitors such as PMSF are used during the manufacturing process.Product Highlights
- Animal origin free
The use of recombinant porcine Trypsin eliminates the potential risk of contamination with virus and any other potential adventitious agents found in animal-derived trypsin. Recombinant Porcine Trypsin 100% animal origin free, and is manufactured in ISO9001 facility.
- Stable
Recombinant porcine trypsin is supplied as sterile lyophilized powder. It minimizes the risk of contamination and activity loss during the process of transport and storage.
- High purity
- Recombinant porcine trypsin provides super purity and specificity.
- No other contaminating proteases such as chymotrypsin or carboxypeptidase A.
Product Details
| CAS # | 9002-07-07 |
| Enzyme Commission # | 3.4.21.4 |
| Molecular weight | 23.8 kDa |
| Origin of species | Porcine |
| Source | E. coli |
| Purified by | HPLC |
| Physical form |
White lyophilized powder |
| Additives | Carbohydrates |
| Protein content | ≥70% β-trypsin, and ≤ 20% α-trypsin |
| Specific activity |
≥ 3800 USP unit/mg protein |
| Unit definition | One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a reaction volume of 3.0 mL at pH7.6 and 25℃, with BAEE as a substrate (1 cm light path). |
| Purity | ≥ 99% |
| Contaminant activity |
No chymotrypsin, carboxypeptidase A, or other protease contaminants. |
| Storage | Store in a sealed container at 2 - 8°C. Once reconstituted, it should be stored at -20°C. The product is stable for 24 months. Greater than 90% enzymatic activity retains after 10 repeated freeze-and-thaw cycles. |
| Intended use | For research or processing only. Not intended for any human or animal diagnostic or therapeutic use. |
Documents
Product Information Sheet
🗎 Recombinant Porcine Trypsin, 100% Animal Original Free
Technical Note
Contact Us
Marvelgent Biosciences Inc.
Head Office
303 Wyman St, Waltham, MA 02451, USA
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1.888.330.6623
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