MagINDEX™ Ni NTA Magnetic Agarose, Settled Resin
High-capacity agarose microspheres with less than 2s magnetic response for 6xHis-tagged proteins. ~40 mg his-tagged protein/mL medium. Ideal for automation, screening, and high-purity results in under an hour. ... Read more
Description
MagINDEX™ Ni-NTA Magnetic Agarose Beads are high-capacity affinity beads engineered for the rapid purification and screening of 6xHis-tagged recombinant proteins. Built on Marvelgent’s proprietary agarose microsphere and surface chemistry platform, these beads combine the high-performance binding of Ni-NTA resin with the speed and simplicity of magnetic separation.
Magnetic bead-based purification streamlines sample processing by eliminating column packing, centrifugation, and gravity-flow handling. Target proteins are captured directly from clarified lysates—whether from bacterial, insect, or mammalian sources—then rapidly separated, washed, and eluted. This format is ideal for:
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Rapid purification of His-tagged proteins (complete in under an hour).
- Parallel processing of multiple samples.
- Small sample volumes where loss must be minimized.
- Automation-compatible workflows where easy handling and reproducibility are essential.
Product Highlights
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Proprietary Agarose Coating
Our unique coating technology minimizes magnetic particle shedding and leaching. This ensures maximum bead integrity without compromising the rapid magnetic responsiveness required for efficient capture and elution. -
Optimized Surface Chemistry
Engineered for efficient ligand coupling and robust Ni-NTA presentation, our surface chemistry provides enhanced capacity for His-tagged proteins. -
Rapid Superparamagnetic Response
The beads respond to magnetic fields in < 2 seconds. This enables quick, clean separation, reduced hands-on time, and efficient processing of multiple samples. -
Superior Purity
By significantly reducing non-specific binding, the optimized MagINDEX™ surface ensures high-purity results in every eluate. -
Versatile Workflow Compatibility
Compatible with standard tubes, multi-well plates, and automated magnetic separation platforms. This flexibility supports everything from individual research to high-throughput screening. -
Broad Buffer Compatibility
Stable in a wide range of common purification buffers, including conditions for both native and denaturing His-tag purification. -
Stringent QC & Lot-to-Lot Consistency
Each lot is manufactured under highly controlled conditions and tested against rigorous quality standards to ensure reproducible performance across every experiment.
| The superparamagnetic core provides anexceptionally fast response to magnetic fields, ensuring rapid separation with no residual magnetism once the field is removed. Our proprietary agarose-based encapsulation provides a robust shield that protects the magnetic core across a wide range of common protein purification reagents. This specialized design ensures high chemical stability while minimizing non-specific binding for superior sample purity. |
Applications
- Rapid purification of His-tagged recombinant proteins.
- Small-scale expression screening to identify high-yield clones.
- High-throughput purification from bacterial, insect, or mammalian lysates.
- Pull-down assays using His-tagged bait proteins to study molecular interactions.
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Native or denaturing purification (refer to specifications for compatible agent ranges).
*Note: No significant decrease in yield after 8 repetitive uses of resin when recommended buffers are used. Refer to Product Details for compatible reagents.
Figure 1. Manual purification of an 80 kDa monomeric recombinant protein. An 80 kDa monomeric His-tagged protein was purified from 50 mL of eukaryotic cell culture spent media using 4 mL of MagINDEX™ Ni-NTA Magnetic Agarose Beads. The sample was incubated for 30 minutes at room temperature with manual magnetic separation. The purified product was eluted with 250 mM imidazole and analyzed by SDS-PAGE under both reducing (+DTT) and non-reducing conditions. The results confirm high-yield recovery and the expected monomeric state of the target protein. Lane 1: Spent medium; Lane 2: Flow-through; Lane 3: Eluate (+DTT); Lane 4: Eluate (non-reduced).
Figure 2. Automated high-throughput purification of His-tagged nanobodies. His6-tagged nanobody clones (1–10; ~15 kDa) were expressed in HEK293 cells. Spent media (2 mL) were incubated with 800 µL of MagINDEX™ Ni-NTA Magnetic Agarose Beads for 30 minutes at room temperature using an automated magnetic separation platform. Samples were eluted with 250 mM imidazole and analyzed by SDS-PAGE. The gel demonstrates consistent recovery and high purity across all ten clones.
Product Details
| Ligand | Nickel |
| Binding capacity | 40 mg 6xHis-tagged protein/mL medium |
| Matrix | Superparamagnetic agarose beads |
| Particle size | 30 μm - 100 μm |
| Concentration | 20% suspension |
| Chemical compatibility |
Reducing agents: Denaturing agents: Detergents: Other additives: Commonly used buffer: |
| Storage temperature |
2 - 8°C |
| Storage buffer |
1x PBS containing 20% ethanol |
Product is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.
Contact Us
Marvelgent Biosciences Inc.
Head Office
303 Wyman St, Waltham, MA 02451, USA
Telephone
1.888.330.6623
Fax
1.888.330.6623
Emails
Ordering support: order.support@marvelgent.com
Technical support: customer.support@marvelgent.com
You are welcome to leave a message using our Contact Form. We will get back to you within 24 hours.
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