PROTEINDEX™ Co-Penta™ Agarose 6 Fast Flow, Chemical Stable
Highly selective, chemical-stable Cobalt-immobilized affinity resin for direct capture of His-tagged proteins from samples containing EDTA, DTT, β-ME, and other chemicals. ... Read more
Description
PROTEINDEX™ Chemical-Tolerant Ni-Penta™ Agarose 6FF is a new immobilized metal ion affinity chromatography (IMAC) medium precharged with cobalt ions, designed mainly for purification of low abundant proteins or challenging proteins in conditions where EDTA, DTT, β-ME, and other stabilizing agents are necessary for maintaining protein stability and integrity.
The cobalt ion binds very strongly to the matrix support in complex with a proprietary pentadentate chelating ligand, which immobilizes the nickel ion through 5 sites to form a homogeneous, chemical-stable structure. This characteristic nickel ion bonding on the Co-Penta™ resin provides excellent resistance to EDTA, NaOH, β-ME, and other reagents that are restricted in the purification protocol using conventional NTA- and IDA- functionalized resins. In particular, Co-Penta™ Agarose 6FF is well suited for direct capture of secreted polyHis-tagged proteins from the cell culture supernatant of eukaryotic cells, including CHO cells, insect High Five™ cells* and Sf9 cells.
Highly cross-linked 6% agarose as the base matrix support offers excellent mechanical stability for automated, scale up purification of histidine-tagged proteins.
Product Highlights
- EDTA-resistant
- High selectivity for high purity
- Retained high 6xHis affinity in various buffers
- Recharge-free with minimal ion leakage
- Requires less imidazole
- NaOH-tolerant, allowing CIP with 1 M NaOH
- Versatile, ready-to-use with convenient formats
Product Details
| Ligand | Cobalt |
| Binding capacity | >10 mg 6x His-tagged protein/mL medium |
| Matrix | Highly cross-linked 6% agarose resin supplied as 50% slurry |
| Particle size | 45 μm - 165 μm |
| Maximum pressure |
0.3 MPa (3 bar) |
| Chemical compatibility |
|
| Storage temperature |
2 - 8°C |
| Storage buffer |
1x PBS containing 20% ethanol |
Product is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.
Contact Us
Marvelgent Biosciences Inc.
Head Office
303 Wyman St, Waltham, MA 02451, USA
Telephone
1.888.330.6623
Fax
1.888.330.6623
Emails
Ordering support: order.support@marvelgent.com
Technical support: cust.support@marvelgent.com
Careers: career@marvelgent.com
You are welcome to leave a message using our Contact Form. We will get back to you within 24 hours.
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