MagINDEX™ rProtein A/G Magnetic Agarose, Settled Resin
High capacity purification of IgGs and Fc-tagged proteins by magnetic separation. Suitable for polyclonal IgG from rabbit, pig, dog and cat serum. >10 mg human IgG/mL resins ... Read more
Description
MagINDEX™ rProtein A/G Magnetic Agarose is an affinity chromatography medium designed for rapid, easy, one-step purification of a wide range of immunoglobulins, as well as Fc region-containing fusion proteins.
Proprietary MagIndex™ technology facilitates encapsulation of the superparamagnetic core in the mechanically stable Agarose matrix, which is the optimal absorbance for high yield protein purification with minimal background.
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The superparamagnetic core has very fast magnetic response in the magnetic field, but no memory after the magnetic force is removed. The agarose-based encapsulation provides a strong shield well protecting the magnetic core in a wide range of reagents that are commonly used in protein purification. Moreover, as an ideal matrix for protein purification, the agarose matrix enables purification of antibodies and Ig fragments with high purity and high capacity, in high throughput screening and various preparative scales.
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Protein A, a bacterial cell wall protein isolated from Staphylococcus aureus, binds to the Fc region of most immunoglobulins through interactions with the heavy chain. Native Protein A contains five high affinity IgG binding domains and many other domains with unknown functions. While the native protein G has 3 IgG binding domains as well as sites for albumin and cell-surface binding, recombinant protein G has only Ig recognition domains but no other non-specific sequences.
Recombinant Protein A/G is a recombinant chimeric protein that is composed of the IgG binding domains of both Protein A and Protein G. rProtein A/G contains five binding domains from Protein A and two from Protein G. The binding of rProtein A/G is less pH-dependent than Protein A, but otherwise has the additive properties of Protein A and G. Therefore, rProtein A/G is ideal for binding the broadest range of IgG subclasses from rabbit, mouse, human and other mammalian samples.
Product Highlights
- High capacity, > 10 mg human IgG / mL of settled resins
- High selectivity for high purity
Applications
- Quick isolation of antibodies from sera and ascites fluids
- Purification of recombinant antibody or scFc fragment from cell cultures
- Depletion of unwanted antibodies and fragments from sample
Product Details
| Ligand | Recombinant chimeric protein A/G containing only Ig binding domains |
| Binding capacity | >10 mg human IgG/mL medium |
| Matrix | Cross-linked agarose beaded particles containing the superparamagnetic core, supplied as a 20% slurry |
| Particle size | 30 μm - 100 μm |
| pH stability |
Long term: pH 2 - 9; Short term: pH 2 - 11 |
| Storage temperature |
2 - 8°C |
| Storage buffer |
PBS containing 0.01% Tween-20 and 0.02% NaN3 |
Product is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.
Documents
Technical Note
🗎 Comparison of Binding Characteristics of Immunoglobulin-Binding Proteins
Contact Us
Marvelgent Biosciences Inc.
Head Office
303 Wyman St, Waltham, MA 02451, USA
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1.888.330.6623
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