High capacity purification of IgGs and Fc-tagged proteins by magnetic separation. Suitable for polyclonal IgG from rabbit, pig, dog and cat serum. >10 mg human IgG/mL resins ... Read more
MagINDEX™ rProtein A Magnetic Agarose is an affinity chromatography medium designed for rapid, easy, one-step purification of immunoglobulins, notably IgG isotypes, as well as Fc region-containing fusion proteins.
Proprietary MagIndex™ technology facilitates encapsulation of the superparamagnetic core in the mechanically stable Agarose matrix, which is the optimal absorbance for high yield protein purification with minimal background.
The superparamagnetic core has very fast magnetic response in the magnetic field, but no memory after the magnetic force is removed. The agarose-based encapsulation provides a strong shield well protecting the magnetic core in a wide range of reagents that are commonly used in protein purification. Moreover, as an ideal matrix for protein purification, the agarose matrix enables purification of antibodies and Ig fragments with high purity and high capacity, in high throughput screening and various preparative scales.
Protein A, a bacterial cell wall protein isolated from Staphylococcus aureus, binds to the Fc region of most immunoglobulins through interactions with the heavy chain. Native Protein A contains five high affinity IgG binding domains and many other domains with unknown functions.
The recombinant Protein A is a genetically engineered protein that contains only the five high affinity IgG binding domains. The non-essential domains were removed to minimize nonspecific binding. The recombinant Protein A is produced in E. coli, and it essentially binds to immunoglobulins with the same degree of affinity as the native Protein A. rProtein A is capable of binding with high affinity to the Fc region from IgG of several species including human and rabbit.
- High capacity, > 10 mg human IgG / mL of settled resins
- High selectivity for high purity
- Quick isolation of antibodies from sera and ascites fluids
- Purification of scFc fragment from cell cultures
- Depletion of unwanted antibodies and fragments from sample
Recombinant scFc protein was isolated from cell culture with MagIndex rProtein A Magnetic Agarose resins. Bound the scFc protein was eluted with 100 mM Glycine, pH 3.0, denoted by the blue arrow. Fractions were analyzed by SDS-PAGE.
Lanes: (L) Culture supernatant; (FT) Flow-through; (E) Elutions 1 and 2.
Sample: 10 mL of culture supernatant containing a scFc fragment (20 µg/mL)
Resin: 0.2 mL settle resins of MagIndex Protein A magnetic agarose
Binding: 45 min at room temperature
Washing: 4 mL binding buffer
Elution: Incubate in 100 mM Glycine, pH 3.0 for 10 min. Repeat 3 times
|Ligand||Recombinant protein A containing only Ig binding domains
|Binding capacity||>10 mg human IgG/mL medium|
|Matrix||Cross-linked agarose beaded particles containing the superparamagnetic core, supplied as a 20% slurry|
|Particle size||30 μm - 100 μm|
Long term: pH 2 - 9;
Short term: pH 2 - 11
||2 - 8°C|
||PBS containing 0.01% Tween-20 and 0.02% NaN3|
Product is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.
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