PROTEINDEX™ NHS-Activated Agarose 4 Fast Flow
Pre-activated agarose matrix for immobilizing amino-containing proteins and peptides. Effective preparation of customized affinity purification resin to isolate specific substances from complex mixtures. ... Read more
PROTEINDEX™ NHS-Activated Agarose 4 Fast Flow is a pre-activated agarose matrix that provides a versatile coupling chemistry for immobilizing amino-containing proteins and peptides.
The pre-activated functional N-hydroxysuccinimide (NHS) group forms a chemically stable amide bone with the ligand that contains a primary amino group. therefore NHS-Activated Agarose 4 Fast Flow can be used to prepare customized affinity purification resin for isolating specific substances from complex mixtures, often achieving very high purity in a single step.
The base matrix of the resin is highly cross-linked 4% agarose, and is ready for application on the automated system, spinning on the centrifuge, and operation under the vacuum.
|Ligand||N-hydroxysuccinimide (NHS) activated
|Coupling capacity||> 10 mg IgG/mL medium|
|Matrix||Highly cross-linked 4% agarose supplied as a 50% slurry|
|Particle size||45 μm - 165 μm, with an average of ~ 90 μm|
||0.3 MPa (3 bar)|
|Recommended flow rate||Gravity flow and medium pressure, 30 - 300 cm/hour|
|Chemical stability (after coupling)
All commonly used aqueous buffers and reagents in the process of antibody purification, including 6 M guanidine-HCl and 8 M urea (ligand dependent)
|pH stability (after coupling)||pH 2 – 13 (ligand dependent)|
|Cleaning-in-place (after coupling)||0.1 M ~ 0.5 M NaOH (ligand dependent)|
|Storage temperature||2 - 8°C|
|Storage buffer (after coupling)
||1x PBS with 20% ethanol|
Product is shipped at ambient temperature. Upon receipt store at 2 - 8°C.
Marvelgent Biosciences Inc.
116 Will Drive, Canton, MA 02021, USA
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