Proprietary engineered alkali-stable rProtein A resin is optimized for purifying mAb w/ high capacity, low leakage. Stable after 200 CIP cycles with 0.1 M NaOH. ... Read more
PROTEINDEX™ rProtein A Agarose 4 Fast Flow is a proprietary affinity chromatography medium designed for easy, one-step purification of immunoglobulins, notably monoclonal antibodies, from samples of larger volumes.
Alkali-tolerant protein A is engineered by gene recombination technology on the base of native protein A. Our Alkali-Tolerant Protein A Agarose 4FF retains ≥95% capacity after 200 times repeated clean-in-place (CIP) cycles with 0.1M NaOH, and approximately 80% of the initial capacity with 0.5M NaOH after 100 times repeated CIP cycles. Alkali-Tolerant Protein A is immobilized onto the cross-linked 4% agarose resin with an orientation that is optimal for immunoglobulin binding. The level of leakage of the ligand during elution is very low.
|Ligand||Recombinant alkali-tolerant protein A produced in E.coli|
|Binding capacity||>40 mg human IgG/mL medium|
|Matrix||Cross-linked 4% agarose supplied as a 50% slurry|
|Particle size||45 μm - 165 μm, with an average of ~ 90 μm|
||0.3 MPa (3 bar)|
|Recommended flow rate||Gravity flow and medium pressure, 30 - 300 cm/hour|
|Chemical stability||All commonly used aqueous buffers and reagents in the process of antibody purification, including 6 M guanidine-HCl and 8 M urea.|
||0.1 M ~ 0.5 M NaOH|
||2 - 8°C|
Product is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.
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