PROTEINDEX™ Alkali-Tolerant Protein A Agarose 4 Fast Flow
Proprietary engineered alkali-stable rProtein A resin is optimized for purifying mAb w/ high capacity, low leakage. Stable after 200 CIP cycles with 0.1 M NaOH. ... Read more
Description
PROTEINDEX™ rProtein A Agarose 4 Fast Flow is a proprietary affinity chromatography medium designed for easy, one-step purification of immunoglobulins, notably monoclonal antibodies, from samples of larger volumes.
Alkali-tolerant protein A is engineered by gene recombination technology on the base of native protein A. Our Alkali-Tolerant Protein A Agarose 4FF retains ≥95% capacity after 200 times repeated clean-in-place (CIP) cycles with 0.1M NaOH, and approximately 80% of the initial capacity with 0.5M NaOH after 100 times repeated CIP cycles. Alkali-Tolerant Protein A is immobilized onto the cross-linked 4% agarose resin with an orientation that is optimal for immunoglobulin binding. The level of leakage of the ligand during elution is very low.
Product Details
| Ligand | Recombinant alkali-tolerant protein A produced in E.coli |
| Binding capacity | >40 mg human IgG/mL medium |
| Matrix | Cross-linked 4% agarose supplied as a 50% slurry |
| Particle size | 45 μm - 165 μm, with an average of ~ 90 μm |
| Maximum pressure |
0.3 MPa (3 bar) |
| Recommended flow rate | Gravity flow and medium pressure, 30 - 300 cm/hour |
| Chemical stability | All commonly used aqueous buffers and reagents in the process of antibody purification, including 6 M guanidine-HCl and 8 M urea. |
| Cleaning-in-place (CIP) |
0.1 M ~ 0.5 M NaOH |
| Storage temperature |
2 - 8°C |
| Storage buffer |
20% ethanol |
Product is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.
Documents
Technical Note
🗎 Comparison of Binding Characteristics of Immunoglobulin-Binding Proteins
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Marvelgent Biosciences Inc.
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