PROTEINDEX™ rProtein A/G Agarose 4 Fast Flow
Versatile purification tool for broad range of immunoglobulins. Less pH-dependent binding protocol. Compatible with FBS, serum albumin. ... Read more
Description
PROTEINDEX™ rProtein A/G Agarose 4FF is an affinity chromatography medium designed for easy, one-step purification of a boarder range of immunoglobulin classes and subclasses. It is versatile, adaptable tool for antibody purification and immunoprecipitation.
Recombinant fusion Protein A/G is a genetically engineered protein (MW ~ 50 kDa) that contains four Fc binding domains from Protein A and two from Protein G and therefore combines the IgG binding profiles of both Protein A and Protein G. The binding of rProtein A/G to immunoglobulins shows less pH-dependent than Protein A. In addition, unlike native Protein A and Protein G that contain albumin-recognition region and binding sites for other proteins, rProtein A/G has these non-immunoglobulin binding sites eliminated, showing super binding specificity for a variety of immunoglobulins.
PROTEINDEX rProtein A/G Agarose 4FF has rProtein A/G immobolized onto highly cross-linked 4% agarose beads via a stable thioether linkage. The coupling technique is optimized to give a high binding capacity for IgG.
Product Highlights
- 10 ~15 mg hIgG/mL resins
- Optimized for immunoprecipitation
- Binds to a broader range of Ig species
- Mechanically stable matrix support, can be used in spin (at up to 1000 g) and automated applications
- Minimal ligand leakage, can be reused
- Versatile, ready-to-use with convenient formats
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Purification of rabbit IgG using rProtein A/G 4FF resin. Lanes: (1) Raw rabbit serum; (2) Flow-through; (3) Elution. |
Product Details
| Ligand | Recombinant fusion protein A/G produced in E.coli |
| Binding capacity | 10 ~ 15 mg human IgG/mL medium |
| Matrix | Highly cross-linked 4% agarose supplied as 50% slurry |
| Particle size | 45 μm - 165 μm |
| Maximum pressure |
0.3 MPa (3 bar) |
| Chemical stability | All commonly used aqueous buffers, including 6 M guanidine-HCl and 8 M urea. |
| Recommended flow rate |
Gravity flow, 100 - 300 cm/hour |
| pH stability | pH3 - 10 |
| Storage temperature | 2 - 8°C |
| Storage buffer | 1x PBS containing 20% ethanol |
Product is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.
Documents
Technical Note
🗎 Comparison of Binding Characteristics of Immunoglobulin-Binding Proteins
Contact Us
Marvelgent Biosciences Inc.
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303 Wyman St, Waltham, MA 02451, USA
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