PROTEINDEX™ rProtein A/G Agarose 4 Fast Flow
Versatile purification tool for broad range of immunoglobulins. Less pH-dependent binding protocol. Compatible with FBS, serum albumin. ... Read more
PROTEINDEX™ rProtein A/G Agarose 4FF is an affinity chromatography medium designed for easy, one-step purification of a boarder range of immunoglobulin classes and subclasses. It is versatile, adaptable tool for antibody purification and immunoprecipitation.
Recombinant fusion Protein A/G is a genetically engineered protein (MW ~ 50 kDa) that contains four Fc binding domains from Protein A and two from Protein G and therefore combines the IgG binding profiles of both Protein A and Protein G. The binding of rProtein A/G to immunoglobulins shows less pH-dependent than Protein A. In addition, unlike native Protein A and Protein G that contain albumin-recognition region and binding sites for other proteins, rProtein A/G has these non-immunoglobulin binding sites eliminated, showing super binding specificity for a variety of immunoglobulins.
PROTEINDEX rProtein A/G Agarose 4FF has rProtein A/G immobolized onto highly cross-linked 4% agarose beads via a stable thioether linkage. The coupling technique is optimized to give a high binding capacity for IgG.
- 10 ~15 mg hIgG/mL resins
- Optimized for immunoprecipitation
- Binds to a broader range of Ig species
- Mechanically stable matrix support, can be used in spin (at up to 1000 g) and automated applications
- Minimal ligand leakage, can be reused
- Versatile, ready-to-use with convenient formats
Purification of rabbit IgG using rProtein A/G 4FF resin.
Lanes: (1) Raw rabbit serum; (2) Flow-through; (3) Elution.
|Ligand||Recombinant fusion protein A/G produced in E.coli|
|Binding capacity||10 ~ 15 mg human IgG/mL medium|
|Matrix||Highly cross-linked 4% agarose supplied as 50% slurry|
|Particle size||45 μm - 165 μm|
||0.3 MPa (3 bar)|
|Chemical stability||All commonly used aqueous buffers, including 6 M guanidine-HCl and 8 M urea.|
|Recommended flow rate
||Gravity flow, 100 - 300 cm/hour|
|pH stability||pH3 - 10|
|Storage temperature||2 - 8°C|
|Storage buffer||1x PBS containing 20% ethanol|
Product is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.
🗎 Comparison of Binding Characteristics of Immunoglobulin-Binding Proteins
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