PROTEINDEX™ rProtein L Agarose 4 Fast Flow
High capacity purification of IgGs, Fab fragments, single-chain Fv fragments and domain antibodies from humans. Suitable for mouse IgM purification. ... Read more
Description
PROTEINDEX™ rProtein L Agarose 4 Fast Flow is an affinity chromatography medium designed for easy, one-step purification of immunoglobulins, as well as Fab, scFv fragments of immunoglobulins from biological fluids and from cell culture media at both laboratory and process scale.
Protein L is first isolated from the surface of bacterial species Peptostreptoccus magnus and now recombinant Protein L is obtained by gene recombination technology. Unlike Protein A and Protein G, which bind primarily through Fc regions of immunoglobulins, Protein L binds the variable region of antibodies kappa light chain and binds a wider range of Ig classes and subclasses. Protein L is therefore particular suitable for capture of a wide range of different-sized antibody fragments such as Fab fragments, single-chain variable fragments (scFv) and domain antibodies (dAbs). It is also suitable for mouse IgM purification from cell.
PROTEINDEX™ rProtein L Agarose 4 Fast Flow utilizes a stable thioether linkage between rProtein L and the cross-linked agarose beads and is optimized for high binding capacity for immunoglobulins.
Product Details
| Ligand | Recombinant protein L produced in E.coli |
| Binding capacity | >15 mg human IgG/mL medium |
| Matrix | Cross-linked 4% agarose supplied as a 50% slurry |
| Particle size | 45 μm - 165 μm |
| Maximum pressure |
0.3 MPa (3 bar) |
| Chemical stability | All commonly used aqueous buffers, including 6 M guanidine-HCl and 8 M urea. |
| Recommended flow rate |
Gravity flow and medium pressure, 30 - 300 cm/hour |
| Storage temperature |
2 - 8°C |
| Storage buffer |
20% ethanol |
Product is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.
Documents
Technical Note
🗎 Comparison of Binding Characteristics of Immunoglobulin-Binding Proteins
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Marvelgent Biosciences Inc.
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